高效液相色谱法同时测定大气PM_(2.5)中15种欧盟优控多环芳烃
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摘要
目的采用高效液相色谱-荧光检测技术,建立大气细颗粒物(PM_(2.5))中15种欧盟优控多环芳烃(EU-PAHs)的同时测定方法。方法利用超声波辅助乙腈提取PM_(2.5)样品中的EU-PAHs,经0.45μm滤膜过滤,用Waters PAH色谱柱分离,用乙腈-水作流动相梯度洗脱,荧光检测器检测,峰面积标准曲线法定量。结果方法线性范围为0.30～84ng/m~3,相关系数均大于0.999,方法精密度为4.6%～10.2%,回收率83.2%～95.6%。结论该方法灵敏度高,准确度高,线性范围宽,适用于大气PM_(2.5)中15种欧盟优控多环芳烃的同时检测。
Objective To establish a simultaneous determination method of 15 EU controlled polycyclic aromatic hydrocarbons(EU-PAHs) in fine particulate matter(PM_(2.5)) by high performance liquid chromatography with fluorescence detector was developed. Methods The EU-PAHs were ultrasound extraction with acetonitrile, then filtered with 0.45μm millipore filter. The separation was accomplished on a Waters PAH column, under gradient elution with the mobile phase composed of acetonitrile and water. Fluorescence detector was used for detection and the quantification was based on peak area standard curves.Results The linear ranges of the 15 EU-PAHs were 0.30-84 ng/m~3 with the correlation coefficients greater than 0.999. The relative standard deviations of the method were 4.6%-10.2%, and the recovery rates were 83.2%-95.6%. Conclusion The method is sensitive, accurate, with wide linear ranges and it is suitable for the simultaneous detection of 15 EU-PAHs in PM_(2.5).
Objective To establish a simultaneous determination method of 15 EU controlled polycyclic aromatic hydrocarbons(EU-PAHs) in fine particulate matter(PM_(2.5)) by high performance liquid chromatography with fluorescence detector was developed. Methods The EU-PAHs were ultrasound extraction with acetonitrile, then filtered with 0.45μm millipore filter. The separation was accomplished on a Waters PAH column, under gradient elution with the mobile phase composed of acetonitrile and water. Fluorescence detector was used for detection and the quantification was based on peak area standard curves.Results The linear ranges of the 15 EU-PAHs were 0.30-84 ng/m~3 with the correlation coefficients greater than 0.999. The relative standard deviations of the method were 4.6%-10.2%, and the recovery rates were 83.2%-95.6%. Conclusion The method is sensitive, accurate, with wide linear ranges and it is suitable for the simultaneous detection of 15 EU-PAHs in PM_(2.5).
引文
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[2]刘大锰,李运勇,蒋佰坤,等.北京首钢地区大气颗粒物中有机污染物的初步研究[J].地球科学-中国地质大学学报,2003,28(3):327-332.
[3]余辉菊,周琛.饮用水中16种多环芳烃的高效液相色谱-荧光-二极管阵列同时测定法[J].环境与健康杂志,2016,33(2):149-152.
[4]王迪,马晶,李灏,等.GC-MS/MS测定土壤中16种多环芳烃[J].环境化学,2018,37(3):617-620.
[5]李荫,柳叶,孙晓伟,等.多环芳烃的样品前处理技术研究进展[J].环境化学,2015,34(8):1460-1469.
[6]何立芳,项小燕,章汝平.超声波萃取-同步荧光法测定土壤中的多环芳烃研究[J].安全与环境学报,2011,11(5):103-108.
[7]刘菲,刘永刚.固相萃取-气相色谱测定地下水中多环芳烃的质量控制研究[J].有色矿冶,2004,20(1):51-55.
[8]李晓敏,张庆华,王璞,等.搅拌子固相吸附-热脱附-气相色谱/质谱/质谱法快速测定空气中多环芳烃[J].分析化学,2011,39(11):1641-1646.
[9]郑红燕,汪利民,谭皓,等.大气中16种多环芳烃化合物高效液相色谱法测定[J].中华劳动卫生职业病杂志,2006,24(9):564-566.
[10] Sikalos TI, Paleologos EK, Karayannis MI. Monitoring of time variation and effect of some meteorological parameters in polynuclear aromatic hydrocarbons in Ioannina, Greece with the aid of HPLC-fluorescence analysis[J]. Talanta, 2002, 58(3):497-510.