非典型皮损及无皮损的生殖道分泌物单纯疱疹病毒DNA检测分析
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摘要
目的探讨非典型皮损及无皮损单纯疱疹病毒(HSV)疑似感染者生殖道分泌物DNA检测的临床意义。方法选取150例疑似HSV感染但无典型症状患者为观察组,以同期150例生殖器典型病变者为对照组,比较2组HSV-Ⅱ型及HSV-Ⅰ型的DNA检测情况。结果观察组HSV检出率20. 00%,显著低于对照组的64. 00%(P <0. 01)。疑似HSV感染男性患者HSV检出率34. 10%,显著低于女性52. 76%(P <0. 05)。结论对非典型皮损或无皮损HSV疑似感染患者,生殖道分泌物DNA检测对HSV感染的诊治及传播的防范具有重要的临床意义。
Objective To explore the clinical significance of DNA detection in genital secretion of suspected herpes simplex virus( HSV) patients with atypical and non-skin lesions. Methods A total of 150 suspected HSV patients with atypical symptoms were selected as observation group,and 150 patients with typical genital lesions in the same period were selected as control group.The DNA detection results of HSV-Ⅱand HSV-Ⅰwere compared between two groups. Results The detection rate of HSV in the observation group was 20. 00%,which was significantly lower than64. 00% in the control group( P < 0. 01). The detection rate of HSV in male patients with suspected HSV infection was 34. 10%,which was significantly lower than 52. 76% in female patients( P <0. 05). Conclusion DNA For suspected HSV patients with atypical and non-skin lesions,detection of DNA in genital secretion has important significance in diagnosis,treatment and prevention of HSV infection.
Objective To explore the clinical significance of DNA detection in genital secretion of suspected herpes simplex virus( HSV) patients with atypical and non-skin lesions. Methods A total of 150 suspected HSV patients with atypical symptoms were selected as observation group,and 150 patients with typical genital lesions in the same period were selected as control group.The DNA detection results of HSV-Ⅱand HSV-Ⅰwere compared between two groups. Results The detection rate of HSV in the observation group was 20. 00%,which was significantly lower than64. 00% in the control group( P < 0. 01). The detection rate of HSV in male patients with suspected HSV infection was 34. 10%,which was significantly lower than 52. 76% in female patients( P <0. 05). Conclusion DNA For suspected HSV patients with atypical and non-skin lesions,detection of DNA in genital secretion has important significance in diagnosis,treatment and prevention of HSV infection.
引文
[1]付玉梅,张晓坤,杨晓仪,等.单纯疱疹病毒抗体检测在生殖器疱疹断中的应用[J].广东医学,2011,32(22):2970-2973.
[2] Lafaille F G,Pessach I M,Zhang S Y,et al. Impaired intrinsic immunity to HSV-1 in human i PSC-derived TLR3deficient CNS cells[J]. Nature,2012,491(7426):769-773.
[3]杨今言,陈赵慧,艾克帕尔·阿布拉江,等.生殖器疱疹病毒感染对免疫水平和性生活的影响研究[J].中华医院感染学杂志,2017,27(20):4607-4609,4613.
[4]杨今言,陈赵慧,艾克帕尔·阿布拉江,等.生殖器疱疹病毒感染对免疫水平和性生活的影响研究[J].中华医院感染学杂志,2017(20):4607-4609.
[5]杨爱琴,卞坤鹏,翟伟,等.生殖器疱疹患者单纯疱疹病毒抗体分布与人乳头瘤病毒及沙眼衣原体感染的相关性研究[J].中华医院感染学杂志,2017(8):1837-1840.
[6] Leroux R G,Clément F,Vandepapelière P,et al. Immunogenicity and safety of different formulations of an adjuvanted glycoprotein D genital herpes vaccine in healthy a-dults:A double-blind randomized trial[J]. Hum Vaccin Immunother,2013,9(6):1254-1262.
[7]曹丽娟,杨斐,李艳,等.生殖器疱疹临床流行病学研究[J].皮肤病与性病,2017,39(4):245-247.
[8]刘振强,戚建明,施林林.膦甲酸钠对复发性生殖器疱疹患者血液Th1/Th2平衡的调节作用[J].实用临床医药杂志,2015,19(17):136-137.
[9]葛帮友,刘影,孙大鹏.探究生殖器疱疹病毒感染对细胞免疫及性功能的影响[J].中国性科学,2016,25(8):71-73.
[10] Elfriede,Agyemang,Quynh A,et al. Performance of Commercial Enzyme-Linked Immunoassays for Diagnosis of Herpes Simplex Virus-1 and Herpes Simplex Virus-2 Infection in a Clinical Setting[J]. Sexually transmitted diseases,2017,44(12):763-767.
[11]唐沛,邹敏,李超,等.胸腺肽与伐昔洛韦治疗生殖器疱疹的临床随机对照研究[J].中国性科学,2016,25(7):84-86.
[12]李雪.生殖器疱疹患者HSV-1和HSV-2感染情况检测及其流行特点分析[J].中国实用医药,2016(2):103-104.
[13]罗辉,陈丽.以单发水疱为表现的复发性生殖器疱疹[J].皮肤病与性病,2017,39(3):190-191.
[14]焦凤萍,刘莉,于爱莲,等. HSV-2NP6与IL-18真核表达载体的构建与表达[J].中国病原生物学杂志,2011,6(6):404-407.
[15]刘雪,邢益平,严玉娟,等.随机扩增多态性技术联合荧光定量高敏检测三种疱疹病毒方法的建立[J].现代生物医学进展,2017,17(21):4032-4037.
[16]宋晓菲,翁伟,甘霖,等.荧光定量PCR检测献血人员外周血白细胞中人疱疹病毒DNA[J].安徽医科大学学报,2017,52(9):1275-1279.
[17]刘微,周艳,赵东海,等.实时荧光定量PCR检测HSV-2方法的建立[J].中国病原生物学杂志,2016(5):397-400.
[18]张传灿.广州市荔湾区2011—2015年性病流行病学分析与防控策略探讨[J].现代诊断与治疗,2017,28(10):1918-1920.
[19]燕伟,自蓉.伐昔洛韦不同药物使用方案在女性复发性生殖疱疹中的疗效对比及安全性研究[J].中国性科学,2017,26(2):57-59.
[20]符文好,刘军麟,陈启红,等.玉屏风颗粒对复发性生殖器疱疹患者血清IFN-γ、IL-4水平的影响研究[J].中国性科学,2017,26(4):102-105.
[21] Matthew R,Pennington,Eric C,et al. New Paradigms for the Study of Ocular Alpha herpesvirus Infections:Insights into the Use of Non-Traditional Host Model Systems[J]. Viruses,2017,9(11):349-350.
[22] Johnston C,Koelle D M,Wald A. HSV-2:in pursuit of avaccine[J]. J Clin Invest,2011,121(12):4600-4609.
[23]汪玉霞,栗敏,白桦.膦甲酸钠联合阿昔洛韦对复发性生殖器疱疹患者免疫功能的影响[J].中国计划生育学杂志,2017,25(7):467-470.
[2] Lafaille F G,Pessach I M,Zhang S Y,et al. Impaired intrinsic immunity to HSV-1 in human i PSC-derived TLR3deficient CNS cells[J]. Nature,2012,491(7426):769-773.
[3]杨今言,陈赵慧,艾克帕尔·阿布拉江,等.生殖器疱疹病毒感染对免疫水平和性生活的影响研究[J].中华医院感染学杂志,2017,27(20):4607-4609,4613.
[4]杨今言,陈赵慧,艾克帕尔·阿布拉江,等.生殖器疱疹病毒感染对免疫水平和性生活的影响研究[J].中华医院感染学杂志,2017(20):4607-4609.
[5]杨爱琴,卞坤鹏,翟伟,等.生殖器疱疹患者单纯疱疹病毒抗体分布与人乳头瘤病毒及沙眼衣原体感染的相关性研究[J].中华医院感染学杂志,2017(8):1837-1840.
[6] Leroux R G,Clément F,Vandepapelière P,et al. Immunogenicity and safety of different formulations of an adjuvanted glycoprotein D genital herpes vaccine in healthy a-dults:A double-blind randomized trial[J]. Hum Vaccin Immunother,2013,9(6):1254-1262.
[7]曹丽娟,杨斐,李艳,等.生殖器疱疹临床流行病学研究[J].皮肤病与性病,2017,39(4):245-247.
[8]刘振强,戚建明,施林林.膦甲酸钠对复发性生殖器疱疹患者血液Th1/Th2平衡的调节作用[J].实用临床医药杂志,2015,19(17):136-137.
[9]葛帮友,刘影,孙大鹏.探究生殖器疱疹病毒感染对细胞免疫及性功能的影响[J].中国性科学,2016,25(8):71-73.
[10] Elfriede,Agyemang,Quynh A,et al. Performance of Commercial Enzyme-Linked Immunoassays for Diagnosis of Herpes Simplex Virus-1 and Herpes Simplex Virus-2 Infection in a Clinical Setting[J]. Sexually transmitted diseases,2017,44(12):763-767.
[11]唐沛,邹敏,李超,等.胸腺肽与伐昔洛韦治疗生殖器疱疹的临床随机对照研究[J].中国性科学,2016,25(7):84-86.
[12]李雪.生殖器疱疹患者HSV-1和HSV-2感染情况检测及其流行特点分析[J].中国实用医药,2016(2):103-104.
[13]罗辉,陈丽.以单发水疱为表现的复发性生殖器疱疹[J].皮肤病与性病,2017,39(3):190-191.
[14]焦凤萍,刘莉,于爱莲,等. HSV-2NP6与IL-18真核表达载体的构建与表达[J].中国病原生物学杂志,2011,6(6):404-407.
[15]刘雪,邢益平,严玉娟,等.随机扩增多态性技术联合荧光定量高敏检测三种疱疹病毒方法的建立[J].现代生物医学进展,2017,17(21):4032-4037.
[16]宋晓菲,翁伟,甘霖,等.荧光定量PCR检测献血人员外周血白细胞中人疱疹病毒DNA[J].安徽医科大学学报,2017,52(9):1275-1279.
[17]刘微,周艳,赵东海,等.实时荧光定量PCR检测HSV-2方法的建立[J].中国病原生物学杂志,2016(5):397-400.
[18]张传灿.广州市荔湾区2011—2015年性病流行病学分析与防控策略探讨[J].现代诊断与治疗,2017,28(10):1918-1920.
[19]燕伟,自蓉.伐昔洛韦不同药物使用方案在女性复发性生殖疱疹中的疗效对比及安全性研究[J].中国性科学,2017,26(2):57-59.
[20]符文好,刘军麟,陈启红,等.玉屏风颗粒对复发性生殖器疱疹患者血清IFN-γ、IL-4水平的影响研究[J].中国性科学,2017,26(4):102-105.
[21] Matthew R,Pennington,Eric C,et al. New Paradigms for the Study of Ocular Alpha herpesvirus Infections:Insights into the Use of Non-Traditional Host Model Systems[J]. Viruses,2017,9(11):349-350.
[22] Johnston C,Koelle D M,Wald A. HSV-2:in pursuit of avaccine[J]. J Clin Invest,2011,121(12):4600-4609.
[23]汪玉霞,栗敏,白桦.膦甲酸钠联合阿昔洛韦对复发性生殖器疱疹患者免疫功能的影响[J].中国计划生育学杂志,2017,25(7):467-470.