病原菌分子生物学诊断技术及其在奶牛子宫内膜炎诊断中的应用进展
|
摘要
子宫内膜炎(Endometritis)是奶牛常见的生殖系统疾病,影响奶牛的生产性能,给奶牛养殖业带来了巨大的经济损失。奶牛子宫内膜炎多由病原菌混合感染,若能及时、准确地检测出致病菌,对该病的综合防控至关重要。目前,奶牛子宫内膜炎病原菌诊断技术的相关报道甚少,为进一步研究开发奶牛子宫内膜炎病原菌诊断技术,论文对目前应用于其诊断的PCR技术、环介导等温扩增(LAMP)技术、基因芯片技术等的研究进展进行了综述,并对其应用情况和优缺点进行了分析与总结,以期为奶牛子宫内膜炎病原菌诊断技术研究提供参考。
Endometritis is a common reproductive disease in dairy cows,affecting the reproductive performance of dairy cows and causing great economic losses to the dairy farming industry.Cow endometritis is usually caused by infections of mixed pathogens.If it can detect the pathogenic bacteria in time and accurately,it is crucial for the comprehensive prevention and control of the disease.At present,there are few reports on the diagnosis of pathogenic bacteria in cow endometritis.The author summarized the current research progress on the polymerase chain reaction(PCR),genechip and loop-mediated isothermal amplification(LAMP),and introduced the application and advantages or disadvantages of rapid detection technology,which aimed to provide some references for research on cow endometritis pathogens.
Endometritis is a common reproductive disease in dairy cows,affecting the reproductive performance of dairy cows and causing great economic losses to the dairy farming industry.Cow endometritis is usually caused by infections of mixed pathogens.If it can detect the pathogenic bacteria in time and accurately,it is crucial for the comprehensive prevention and control of the disease.At present,there are few reports on the diagnosis of pathogenic bacteria in cow endometritis.The author summarized the current research progress on the polymerase chain reaction(PCR),genechip and loop-mediated isothermal amplification(LAMP),and introduced the application and advantages or disadvantages of rapid detection technology,which aimed to provide some references for research on cow endometritis pathogens.
引文
[1] Sheldon I M,Lewisg S,Leblancee S,et al.Defining postpartum uterine disease in cattle[J].Theriogenology,2006,65(8):1516-1530.
[2] Dohmen M J W,Joop K,Sturk A,et al.Relationship between intra-uterine bacterial contamination,endotoxin levels and the development of endometritis in postpartum cows with dystocia or retained placenta[J].Theriogenology,2000,54(7):1019-1032.
[3] Azawi O I.Postpartum uterine infection in cattle[J].Anim Reprod Sci,2008,105(3/4):187-208.
[4] Leblanc S.Economics of improving reproductive performance in dairy herds[J].Adv Dairy Technol,2007,19:201-214.
[5]龚团莲.奶牛子宫内膜炎致病菌的分离鉴定、药敏试验及致病力研究[J].2017,38(6):104-106.
[6] Aghamiri S M,Haghkhah M,Ahmadi M R,et al.Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows[J].Reprod Dom Anim,2014,49(2):233-238.
[7] Bicalho M L,Machado V S,Oikonomou G,et al.Association between virulence factors of Escherichia coli,Fusobacterium necrophorum,and Arcanobacterium pyogenes and uterine diseases of dairy cows[J].Vet Microbiol,2012,157(1/2):125-131.
[8]马建玲.奶牛子宫内膜炎病原菌的分离鉴定及药敏试验[J].安徽农业科学,2014(14):4371-4373.
[9] Santos T M,Gilbert R O,Bicalho R C.Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows[J].J Dairy Sci,2011,94(1):291-302.
[10] Guo M Y,Wang G Q,Shao G,et al.Identification of intrauterine Mycoplasmain endometritis of dairy cow isolates by 16S rDNA nested PCR-DGGE[J].J Anim Vet Adv,2012,11(10):1745-1752.
[11]管远志,王艾琳,李座.医学微生物实验技术[M].北京:化学工业出版社,2006:249-255.
[12] Liu M C,Wu C M,Liu Y C,et al.Identification,susceptibility,and detection of integron-gene cassettes of Arcanobacterium pyogenes in bovine endometritis[J].J Dairy Sci,2009,92(8):3659-3666.
[13]王爽,奶牛子宫内膜炎主要病原菌分离鉴定及多重PCR诊断方法的建立[D].黑龙江大庆:黑龙江八一农垦大学,2008.
[14]刘明春,杨蕴力,吴聪明,等.奶牛子宫内膜炎化脓隐秘杆菌的分离与PCR鉴定[J].畜牧与兽医,2008,40(11):76-77.
[15] Yan Y,Wang X,Guo Y,et al.Study on detection of Salmonellain poultry samples by real-time PCR with Taqman probes[J].Zhonghua Yu Fang Yi Xue Za Zhi,2014,48(8):731-735.
[16]张明富.牛源化脓隐秘杆菌的分离鉴定及其PCR检测方法的建立[D].黑龙江哈尔滨:东北农业大学,2012.
[17] Aghamiri S M,Haghkhah M,Ahmadi M R,et al.Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows[J].Rep Dom Anim,2014,49:233-238.
[18] Tramuta C,Lacerenza D,Zoppi S,et al.Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples[J].J Vet Diag Invest,2011,23(4):657-664.
[19] Gillespie B E,Oliver S P.Simultaneous detection of mastitis pathogens,Staphylococcus aureus,Streptococcus uberis,and Streptococcus agalactiae by multiplex real-time polymerase chain reaction[J].J Dairy Sci,2005,88(10):3510-3518.
[20]王丹,杨峰,李宏胜,等.奶牛乳房炎病原菌诊断技术研究进展[J].中国畜牧兽医,2017,44(6):1811-1817.
[21] Sun D,Wu R,He X,et al.Development of a multiplex PCR for diagnosis of Staphylococcus aureus,Escherichia coli and Bacillus cereus from cows with endometritis[J].Agri Sci China,2011,10(10):1624-1629.
[22]萨姆布鲁克J,拉塞尔著DW.分子克隆实验指南[M].3版.黄培堂,等.译.北京:科学出版社,2003.
[23] Guion C E,Ochoa T J,Walker C M,et al.Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR[J].J Clin Microbiol,2008,46(5):1752-1757.
[24]Wang J,Sun C G,Liu C,et al.Comparison of vaginal microbial community structure in healthy and endometritis dairy cows by PCR-DGGE and real-time PCR[J].Anaerobe,2016,38:1-6.
[25]唐吉思,布日额,吴金花,等.荧光定量PCR检测牛乳中金黄色葡萄球菌肠毒素A基因方法的建立[J].中国预防兽医学报,2012,34(1):56-59.
[26] Shao Y,Zhu S,Jin C,et al.Development of multiplex loop-mediated isothermal amplification-RFLP(mLAMP-RFLP)to detect Salmonella spp.and Shigella spp.in milk[J].Int J Food Microbiol,2011,148(2):75-79.
[27] Kokkinos P A,Ziros P G,Bellou M,et al.Loop-mediated isothermal amplification(LAMP)for the detection of Salmonellain food[J].Food Anal Meth,2014,7(2):512-526.
[28] Cho A R,Dong H J,Seo K H,et al.Development of a loopmediated isothermal amplification assay for detecting Listeria monocytogenes prfA in Milk[J].Food Sci Biotechnol,2014,23(2):467-474.
[29] Sheet O H,Grabowski N T,Abdulmawjood A,et al.Development and validation of a loop mediated isothermal amplification(LAMP)assay for the detection of Staphylococcus aureus in bovine mastitis milk samples[J].Mol Cell Probes,2016(30):320e325.
[30]李永刚,王德国,武建刚,等.环介导恒温扩增法(LAMP)检测金黄色葡萄球菌[J].食品工业科技,2010,31(1):388-390.
[31] Miller S M,Tourlousse D M,Stedtfeld R D,et al.In situsynthesized virulence and marker gene biochip for detection of bacterial pathogens in water[J].Appl Environ Microbiol,2008,74(7):2200-2209.
[32]饶宝,唐桂芬,刘玲玲,等.沙门氏菌,金黄色葡萄球菌和大肠杆菌的基因芯片检测技术研究[J].郑州牧业工程高等专科学校学报,2012,32(3):3-5.
[33] Lee K H,Lee J W,Wang S W,et al.Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples[J].J Vet Diagn Invest,2008,20(4):463-471.
[2] Dohmen M J W,Joop K,Sturk A,et al.Relationship between intra-uterine bacterial contamination,endotoxin levels and the development of endometritis in postpartum cows with dystocia or retained placenta[J].Theriogenology,2000,54(7):1019-1032.
[3] Azawi O I.Postpartum uterine infection in cattle[J].Anim Reprod Sci,2008,105(3/4):187-208.
[4] Leblanc S.Economics of improving reproductive performance in dairy herds[J].Adv Dairy Technol,2007,19:201-214.
[5]龚团莲.奶牛子宫内膜炎致病菌的分离鉴定、药敏试验及致病力研究[J].2017,38(6):104-106.
[6] Aghamiri S M,Haghkhah M,Ahmadi M R,et al.Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows[J].Reprod Dom Anim,2014,49(2):233-238.
[7] Bicalho M L,Machado V S,Oikonomou G,et al.Association between virulence factors of Escherichia coli,Fusobacterium necrophorum,and Arcanobacterium pyogenes and uterine diseases of dairy cows[J].Vet Microbiol,2012,157(1/2):125-131.
[8]马建玲.奶牛子宫内膜炎病原菌的分离鉴定及药敏试验[J].安徽农业科学,2014(14):4371-4373.
[9] Santos T M,Gilbert R O,Bicalho R C.Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows[J].J Dairy Sci,2011,94(1):291-302.
[10] Guo M Y,Wang G Q,Shao G,et al.Identification of intrauterine Mycoplasmain endometritis of dairy cow isolates by 16S rDNA nested PCR-DGGE[J].J Anim Vet Adv,2012,11(10):1745-1752.
[11]管远志,王艾琳,李座.医学微生物实验技术[M].北京:化学工业出版社,2006:249-255.
[12] Liu M C,Wu C M,Liu Y C,et al.Identification,susceptibility,and detection of integron-gene cassettes of Arcanobacterium pyogenes in bovine endometritis[J].J Dairy Sci,2009,92(8):3659-3666.
[13]王爽,奶牛子宫内膜炎主要病原菌分离鉴定及多重PCR诊断方法的建立[D].黑龙江大庆:黑龙江八一农垦大学,2008.
[14]刘明春,杨蕴力,吴聪明,等.奶牛子宫内膜炎化脓隐秘杆菌的分离与PCR鉴定[J].畜牧与兽医,2008,40(11):76-77.
[15] Yan Y,Wang X,Guo Y,et al.Study on detection of Salmonellain poultry samples by real-time PCR with Taqman probes[J].Zhonghua Yu Fang Yi Xue Za Zhi,2014,48(8):731-735.
[16]张明富.牛源化脓隐秘杆菌的分离鉴定及其PCR检测方法的建立[D].黑龙江哈尔滨:东北农业大学,2012.
[17] Aghamiri S M,Haghkhah M,Ahmadi M R,et al.Development of a multiplex PCR for the identification of major pathogenic bacteria of post-partum endometritis in dairy cows[J].Rep Dom Anim,2014,49:233-238.
[18] Tramuta C,Lacerenza D,Zoppi S,et al.Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples[J].J Vet Diag Invest,2011,23(4):657-664.
[19] Gillespie B E,Oliver S P.Simultaneous detection of mastitis pathogens,Staphylococcus aureus,Streptococcus uberis,and Streptococcus agalactiae by multiplex real-time polymerase chain reaction[J].J Dairy Sci,2005,88(10):3510-3518.
[20]王丹,杨峰,李宏胜,等.奶牛乳房炎病原菌诊断技术研究进展[J].中国畜牧兽医,2017,44(6):1811-1817.
[21] Sun D,Wu R,He X,et al.Development of a multiplex PCR for diagnosis of Staphylococcus aureus,Escherichia coli and Bacillus cereus from cows with endometritis[J].Agri Sci China,2011,10(10):1624-1629.
[22]萨姆布鲁克J,拉塞尔著DW.分子克隆实验指南[M].3版.黄培堂,等.译.北京:科学出版社,2003.
[23] Guion C E,Ochoa T J,Walker C M,et al.Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR[J].J Clin Microbiol,2008,46(5):1752-1757.
[24]Wang J,Sun C G,Liu C,et al.Comparison of vaginal microbial community structure in healthy and endometritis dairy cows by PCR-DGGE and real-time PCR[J].Anaerobe,2016,38:1-6.
[25]唐吉思,布日额,吴金花,等.荧光定量PCR检测牛乳中金黄色葡萄球菌肠毒素A基因方法的建立[J].中国预防兽医学报,2012,34(1):56-59.
[26] Shao Y,Zhu S,Jin C,et al.Development of multiplex loop-mediated isothermal amplification-RFLP(mLAMP-RFLP)to detect Salmonella spp.and Shigella spp.in milk[J].Int J Food Microbiol,2011,148(2):75-79.
[27] Kokkinos P A,Ziros P G,Bellou M,et al.Loop-mediated isothermal amplification(LAMP)for the detection of Salmonellain food[J].Food Anal Meth,2014,7(2):512-526.
[28] Cho A R,Dong H J,Seo K H,et al.Development of a loopmediated isothermal amplification assay for detecting Listeria monocytogenes prfA in Milk[J].Food Sci Biotechnol,2014,23(2):467-474.
[29] Sheet O H,Grabowski N T,Abdulmawjood A,et al.Development and validation of a loop mediated isothermal amplification(LAMP)assay for the detection of Staphylococcus aureus in bovine mastitis milk samples[J].Mol Cell Probes,2016(30):320e325.
[30]李永刚,王德国,武建刚,等.环介导恒温扩增法(LAMP)检测金黄色葡萄球菌[J].食品工业科技,2010,31(1):388-390.
[31] Miller S M,Tourlousse D M,Stedtfeld R D,et al.In situsynthesized virulence and marker gene biochip for detection of bacterial pathogens in water[J].Appl Environ Microbiol,2008,74(7):2200-2209.
[32]饶宝,唐桂芬,刘玲玲,等.沙门氏菌,金黄色葡萄球菌和大肠杆菌的基因芯片检测技术研究[J].郑州牧业工程高等专科学校学报,2012,32(3):3-5.
[33] Lee K H,Lee J W,Wang S W,et al.Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples[J].J Vet Diagn Invest,2008,20(4):463-471.