分子诊断技术在结核及耐药检测中的研究进展
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摘要
结核病是由结核分支杆菌感染引起的呼吸道传染病,具有患病率高、死亡率高、感染率高、病例发现率低等的特点,目前的实验室诊断方法多样,但传统方法存在特异性差、耗时长、灵敏度较低等缺点,于是高灵敏度和高特异性兼具的分子诊断技术发展迅速,本文就分子诊断技术在结核及耐药检测中的应用情况作一综述。
Tuberculosis(TB) is a respiratory infectious disease caused by Mycobacterium tuberculosis, with high prevalence rate, high mortality and high infection rate and low case detection rate. The current laboratory diagnostic methods of TB were diverse. Because the traditional method has poor specificity, low sensitivity and time-consuming, molecular diagnosis technology with high sensitivity and high specificity is developing rapidly. This review describes application of the molecular diagnostic technology in the diagnosis of TB and drug-resistant TB detection.
Tuberculosis(TB) is a respiratory infectious disease caused by Mycobacterium tuberculosis, with high prevalence rate, high mortality and high infection rate and low case detection rate. The current laboratory diagnostic methods of TB were diverse. Because the traditional method has poor specificity, low sensitivity and time-consuming, molecular diagnosis technology with high sensitivity and high specificity is developing rapidly. This review describes application of the molecular diagnostic technology in the diagnosis of TB and drug-resistant TB detection.
引文
[1]毛五一.分子信标荧光定量PCR技术检测结核分枝杆菌方法建立及其临床应用[J].心理医生,2015,21(16):118-119.
[2]来汉江,佘军,杨莉俊,等.TaqMan-MGB探针实时荧光PCR检测结核分枝杆菌北京基因型的研究[J].中国卫生检验杂志,2017,27(7):965-967.
[3]侯杏芳,孟娟,张国良,等.肺结核患者外周血单个核细胞中miRNA-150的表达及意义[J].临床肺科杂志,2016,21(3):501-504.
[4]李晓非,梁桂亮,普冬,等.基因芯片技术在分枝杆菌菌种鉴定和结核耐药性检测中的应用及评价[J].中国实验诊断学,2015,19(2):204-207.
[5]胡晓红,向启云,庄倩,等.基因芯片法检测结核分枝杆菌耐药性的可行性分析[J].国际检验医学杂志,2015,36(23):3421-3422,3425.
[6]刘旻雁,白冰,陈晋.基于核酸扩增技术的分枝杆菌检测及耐药分析技术的发展[J].检验医学,2016,31(5):426-432.
[7]Maurya AK,Singh AK,Kant S,et al.Use of GenoType MTBDRplus assay to assess drug resistance and mutation patterns of multidrug-resistant tuberculosis isolates in northern India[J].Indian J Med Microbiol,2013,31(3):230-236.
[8]张国钦,巨韩芳,钟达,等.Xpert MTB/RIF应用于可疑肺结核患者诊断效果分析[J].中国热带医学,2017,17(3):266-269.
[9]王德翠,陈子芳,陈琦,等.GeneXpert MTB/RIF技术在结核性胸膜炎诊断及利福平耐药检测中的价值[J].国际呼吸杂志,2015,35(14):1071-1074.
[10]杨辉,汪文斐,张国良,等.高分辨率熔解曲线分析技术检测结核分枝杆菌的耐药性[J].中国热带医学,2014,14(12):1435-1439.
[11]Sharma K,Modi M,Kaur H,et al.rpo B gene high-resolution melt curve analysis:a rapid approach for diagnosis and screening of drug resistance in tuberculous meningitis[J].Diagn Microbiol Infect Dis,2015,83(2):144-149.
[12]杨振兴,陈威,许敏,等.五种检测方法在结核病诊断中的应用评价[J].中国热带医学,2016,16(10):1024-1026.
[13]王静,刘立宾,岳永宁,等.RNA恒温扩增实时检测技术与荧光定量PCR联合检测肺泡灌洗液对痰涂阴性肺结核的快速诊断价值[J].中华医院感染学杂志,2017,27(2):300-304.
[14]Lin SY,Rodwell TC,Victor TC,et al.Pyrosequencing for rapid detection of extensively drug-Resistant Mycobacterium tuberculosis in clinical isolates and clinical specimens[J].J Clin Microbiol,2014,52(2):475-482.
[15]杨彩虹,曹旭东,史静雪,等.结核分枝杆菌临床分离株链霉素耐药性的检测及耐药基因突变位点分析[J].石河子大学学报:自科版,2017,35(2):169-175.
[16]Zhang H,Li D,Zhao L,et al.Genome sequencing of 161Mycobacterium tuberculosis isolates from China identifies genes and intergenic regions associated with drug resistance[J].Na Genet,2013,45(10):1255-1260.
[17]Safi H,Lingaraju S,Amin A,et al.Evolution of high-leve ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-[beta]-D-arabinose biosynthetic and utilization pathway genes[J].Nat Genet,2013,45(10):1190-1197.
[18]Tahlan K,Wilson R,Kastrinsky DB,et al.SQ109 targets mmpL3,a membrane transporter of trehalosemonomycolate involved in mycolic Acid Donation to the Cell wall core of mycobacterium tuberculosis[J].Antimicrob Agents Chemother,2012,56(4):1797-1809.
[19]Sharma K,Verma R,Advani J,et al.Whole Genome Sequencing of Mycobacterium tuberculosis Isolates From Extrapulmonary Sites[J].OMICS,2017,21(7):413-425.
[20]黄雪静,李翠萍,李洪涛,等.蛋白组技术发现和验证耐多药肺结核潜在血清标志物FCN3[J].中国卫生检验杂志,2016,26(18):2596-2599.
[21]王琳,翁丽珍,陈晓红,等.应用蛋白质指纹图谱技术快速鉴别诊断菌阴肺结核[J].中国人兽共患病学报,2014,30(7):688-691.
[22]Hussain MM,Samir TM,Azzazy HM.Unmodified gold nanoparticles for direct and rapid detection of Mycobacterium tuberculosis complex[J].Clin Biochem,2013,46(7-8):633-637.
[23]Lara-Oya A,Mendoza-Lopez P,Rodriguez-Granger J,et al.Evaluation of the speed-oligo direct Mycobacterium tuberculosis assay for molecular detection of mycobacteria in clinical respiratory specimens[J].J Clin Microbiol,2013,51(1):77-82.
[2]来汉江,佘军,杨莉俊,等.TaqMan-MGB探针实时荧光PCR检测结核分枝杆菌北京基因型的研究[J].中国卫生检验杂志,2017,27(7):965-967.
[3]侯杏芳,孟娟,张国良,等.肺结核患者外周血单个核细胞中miRNA-150的表达及意义[J].临床肺科杂志,2016,21(3):501-504.
[4]李晓非,梁桂亮,普冬,等.基因芯片技术在分枝杆菌菌种鉴定和结核耐药性检测中的应用及评价[J].中国实验诊断学,2015,19(2):204-207.
[5]胡晓红,向启云,庄倩,等.基因芯片法检测结核分枝杆菌耐药性的可行性分析[J].国际检验医学杂志,2015,36(23):3421-3422,3425.
[6]刘旻雁,白冰,陈晋.基于核酸扩增技术的分枝杆菌检测及耐药分析技术的发展[J].检验医学,2016,31(5):426-432.
[7]Maurya AK,Singh AK,Kant S,et al.Use of GenoType MTBDRplus assay to assess drug resistance and mutation patterns of multidrug-resistant tuberculosis isolates in northern India[J].Indian J Med Microbiol,2013,31(3):230-236.
[8]张国钦,巨韩芳,钟达,等.Xpert MTB/RIF应用于可疑肺结核患者诊断效果分析[J].中国热带医学,2017,17(3):266-269.
[9]王德翠,陈子芳,陈琦,等.GeneXpert MTB/RIF技术在结核性胸膜炎诊断及利福平耐药检测中的价值[J].国际呼吸杂志,2015,35(14):1071-1074.
[10]杨辉,汪文斐,张国良,等.高分辨率熔解曲线分析技术检测结核分枝杆菌的耐药性[J].中国热带医学,2014,14(12):1435-1439.
[11]Sharma K,Modi M,Kaur H,et al.rpo B gene high-resolution melt curve analysis:a rapid approach for diagnosis and screening of drug resistance in tuberculous meningitis[J].Diagn Microbiol Infect Dis,2015,83(2):144-149.
[12]杨振兴,陈威,许敏,等.五种检测方法在结核病诊断中的应用评价[J].中国热带医学,2016,16(10):1024-1026.
[13]王静,刘立宾,岳永宁,等.RNA恒温扩增实时检测技术与荧光定量PCR联合检测肺泡灌洗液对痰涂阴性肺结核的快速诊断价值[J].中华医院感染学杂志,2017,27(2):300-304.
[14]Lin SY,Rodwell TC,Victor TC,et al.Pyrosequencing for rapid detection of extensively drug-Resistant Mycobacterium tuberculosis in clinical isolates and clinical specimens[J].J Clin Microbiol,2014,52(2):475-482.
[15]杨彩虹,曹旭东,史静雪,等.结核分枝杆菌临床分离株链霉素耐药性的检测及耐药基因突变位点分析[J].石河子大学学报:自科版,2017,35(2):169-175.
[16]Zhang H,Li D,Zhao L,et al.Genome sequencing of 161Mycobacterium tuberculosis isolates from China identifies genes and intergenic regions associated with drug resistance[J].Na Genet,2013,45(10):1255-1260.
[17]Safi H,Lingaraju S,Amin A,et al.Evolution of high-leve ethambutol-resistant tuberculosis through interacting mutations in decaprenylphosphoryl-[beta]-D-arabinose biosynthetic and utilization pathway genes[J].Nat Genet,2013,45(10):1190-1197.
[18]Tahlan K,Wilson R,Kastrinsky DB,et al.SQ109 targets mmpL3,a membrane transporter of trehalosemonomycolate involved in mycolic Acid Donation to the Cell wall core of mycobacterium tuberculosis[J].Antimicrob Agents Chemother,2012,56(4):1797-1809.
[19]Sharma K,Verma R,Advani J,et al.Whole Genome Sequencing of Mycobacterium tuberculosis Isolates From Extrapulmonary Sites[J].OMICS,2017,21(7):413-425.
[20]黄雪静,李翠萍,李洪涛,等.蛋白组技术发现和验证耐多药肺结核潜在血清标志物FCN3[J].中国卫生检验杂志,2016,26(18):2596-2599.
[21]王琳,翁丽珍,陈晓红,等.应用蛋白质指纹图谱技术快速鉴别诊断菌阴肺结核[J].中国人兽共患病学报,2014,30(7):688-691.
[22]Hussain MM,Samir TM,Azzazy HM.Unmodified gold nanoparticles for direct and rapid detection of Mycobacterium tuberculosis complex[J].Clin Biochem,2013,46(7-8):633-637.
[23]Lara-Oya A,Mendoza-Lopez P,Rodriguez-Granger J,et al.Evaluation of the speed-oligo direct Mycobacterium tuberculosis assay for molecular detection of mycobacteria in clinical respiratory specimens[J].J Clin Microbiol,2013,51(1):77-82.