渔用疫苗灭活剂研究进展
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摘要
渔用疫苗是防治鱼类养殖疾病最有效的方法之一。其中,灭活疫苗是最主要的商品化疫苗,其制备的关键为病原的有效灭活。正确的灭活方法不仅可使病原完全失去活性,还能不破坏抗原,从而制备高效疫苗。化学试剂灭活是最常用的灭活方法。本文介绍了当前渔用疫苗中常见的灭活剂种类、特点、灭活机制、研究应用现状等,并对灭活剂的未来研究方向进行了展望。
Fish vaccine is one of the most effective ways to prevent fish from diseases. Inactivated vaccine is the most important kind of the commercial fish vaccines,of which the effective pathogen inactivation is the key to preparation. A correct inactivation method may inactivate the pathogens completely while does not destroy the antigens so as to prepare highly effective vaccines. Inactivation with chemical reagents is the most popular inactivation method. In this paper,the type,characteristics,inactivation mechanism and application status of inactivated agents are reviewed,and the research directions of inactivating agents in the future are prospected.
Fish vaccine is one of the most effective ways to prevent fish from diseases. Inactivated vaccine is the most important kind of the commercial fish vaccines,of which the effective pathogen inactivation is the key to preparation. A correct inactivation method may inactivate the pathogens completely while does not destroy the antigens so as to prepare highly effective vaccines. Inactivation with chemical reagents is the most popular inactivation method. In this paper,the type,characteristics,inactivation mechanism and application status of inactivated agents are reviewed,and the research directions of inactivating agents in the future are prospected.
引文
[1] Food and Agriculture of the United Nations. The state of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all[R/OL].(2016-07-15)[2018-01-20]. http://www.fao.org/3/a-i5555e.pdf.联合国粮食及农业组织. 2016年世界渔业和水产养殖状况:为全面实现粮食和营养安全做贡献[R/OL]. http://www.fao.org/fsnforum/zh/resources/fsn.resources/%E3%80%8A2016%E5%B9%B4%E4%B8%96%E7%95%8C%E6%B8%94%E4%B8%9A%E5%92%8C%E6%B0%B4%E4%BA%A7%E5%85%BB%E6%AE%96%E7%8A%B6%E5%86%B5%E3%80%8B.
[2]农业部渔业渔政管理局. 2016年全国渔业经济统计公报[R/OL].(2017-07-25)[2018-01-20]. http://www.cnfm.gov.cn/yyywyzj/201707/t20170725_5759859.htm.
[3] OU-YANG Z,WANG P,HUANG X,et al. Immunogenicity and protective effects of inactivated Singapore grouper iridovirus(SGIV)vaccines in orange-spotted grouper,Epinephelus coioides[J]. Dev Comp Immunol,2012,38(2):254-261.
[4] WU S Q,TAO J F,GONG H,et al. Situation and tendency of the fishery vaccine development[J]. Chin Fish Qual Stand,2014,4(1):1-13.(in Chinese)吴淑勤,陶家发,巩华,等.渔用疫苗发展现状及趋势[J].中国渔业质量与标准,2014,4(1):1-13.
[5]李超,蒋梦娜,丁兆钧,等.渔用疫苗的研究进展[J].农业开发与装备,2015,11(7):72-72,110.
[6] ZHANG Y,ZHOU L,ZHANG Y. Investigation of UV. TiO2 photocatalysis and its mechanism in Bacillus subtilis spore inactivation[J]. J Environ Sci(China),2014,26(9):1943-1948.
[7] HUSSAIN I,PRICE G W,FARID A H. Inactivation of Aleutian mink disease virus through high temperature exposure in vitro and under field-based composting conditions[J]. Vet Microbiol,2014,173(1-2):50-58.
[8] LI J,SUO Y,LIAO X,et al. Analysis of Staphylococcus aureus cell viability,sublethal injury and death induced by synergistic combination of ultrasound and mild heat[J]. Ultrason Sonochem,2017,39(11):101-110.
[9] BOUDARKOV V A,SEREDA A D,CARPOV O N,et al.Using gamma rays to inactivate African swine fever virus[J].Russian Agri Sci,2016,42(5):375-377.
[10] GERALD O. Some bacteria are not inactivated by ultrasound:here's why[J]. Chem Engi,2013,120(9):16.
[11] HABIB M,HUSSAIN I,FANG W H,et al. Inactivation of infectious bursal disease virus by binary ethylenimine and formalin[J]. J Zhejiang Univ Sci B,2006,7(4):320-323.
[12] MORROW W J W,SHEIKH N A,SCHMIDT C S,et al. Vaccinology:principles and practice[M]. New Jersey:Wiley-Blackwell,2012,44(1. 3):224-236.
[13] JEONG Y U,SUBRAMANIAN D,YEOUNGHWAN J,et al.Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder,Paralichthys olivaceus[J]. Arch Polish Fisher,2016,24(1):23-32.
[14] AMANNA I J,RAUéH P,SLIFKA M K. Development of a new hydrogen peroxide-based vaccine platform[J]. Nat Med,2012,18(6):974-979.
[15] SEO H S. Application of radiation technology in vaccines development[J]. Clin Exp Vac Res,2015,4(2):145-158.
[16] METZ B,KERSTEN G P,HOOGERHOUT P,et al. Identification of formaldehyde-induced modifications in proteins:reactions with model peptides[J]. J Biol Chem,2004,279(8):6235-6243.
[17] SRINIVASA S,DING X,KAST J. Formaldehyde cross-linking and structural proteomics:Bridging the gap[J]. Methods,2015,89(1):91-98.
[18] PAWAR S D,MURTADAK V B,KALE S D,et al. Evaluation of different inactivation methods for high and low pathogenic avian influenza viruses in egg-fluids for antigen preparation[J]. J Virol Methods,2015,222(1):28-33.
[19] GONG W B,HAO W W,LIAO W P,et al. Inactivation effect of genotypeⅦNewcastle virus with two different inactivants[J]. Sichuan Anim Vet Sci,2017,44(10):21-23.(in Chinese)龚文波,郝伟伟,廖韦萍,等.两种不同灭活剂对基因Ⅶ型新城疫病毒灭活效果的影响研究[J].四川畜牧兽医,2017,44(10):21-23.
[20] MA Q R,ZHANG L,YANG X,et al. Progress in research on determination methods for residual formaldehyde content in vaccines[J]. Chin J Biologicals,2017,30(12):1351-1353,1358.(in Chinese)马秋冉,张璐,杨星,等.疫苗中甲醛残留量检测方法的研究进展[J].中国生物制品学杂志,2017,30(12):1351-1353,1358.
[21] H魧NNINE O,KNOL A B,JANTUNEN M,et al. Environmental burden of disease in Europe:assessing nine risk factors in six countries[J]. Environ Health Perspect,2014,122(5):439-446.
[22] NIELSEN G D,LARSEN S T,WOLKOFF P. Re-evaluation of the WHO(2010)formaldehyde indoor air quality guideline for cancer risk assessment[J]. Arch Toxicol,2017,91(1):35-61.
[23] NILSEM H,LILLEHAUG A,TAKSDAL T,et al. Toxicity of intraperitoneally injected formalin in Atlantic salmon,Salmo salar L[J]. J Fish Dis,2010,15(4):323-329.
[24] XU S Z,YIN Y B,WANG X. Research progress on routine inactivants in veterinary vaccines[J]. China Anim Husb Vet Med,2010,37(9):162-167.(in Chinese)徐守振,尹燕博,王新.动物疫苗中常用抗原灭活剂的研究进展[J].中国畜牧兽医,2010,37(9):162-167.
[25] PERRIN P,MORGEAUX S. Inactivation of DNA byβ-propiolactone[J]. Biologicals,1995,23(3):207-211.
[26] DELRUE I,VERZELE D,MADDER A,et al. Inactivated virus vaccines from chemistry to prophylaxis:merits,risks and challenges[J]. Expert Rev Vaccines,2012,11(6):695-719.
[27] JIANG L M,LIN X B,GAO L,et al. Inactivated of rabies virus withβ-propiolactone[J]. Int J Epidemiol Infect Dis,2014,41(2):137-139.(in Chinese)姜立民,林晓波,高磊,等.β.丙内酯对狂犬病病毒的灭活效果[J].国际流行病学传染病学杂志,2014,41(2):137-139.
[28] LIU Z W,LI F A,QIAN H. Study for application ofβ-propiolactone on preparation of human rabies vaccine of purified hamster kidney cell[J]. Chin J Zoon,1999,15(6):71-72.(in Chinese)刘兆文,李福安,钱浩.β-丙内酯在人用纯化地鼠肾细胞狂犬疫苗制备中的应用研究[J].中国人兽共患病学报,1999,15(6):71-72.
[29] YANG D K,HAHYUN K,JINJU N,et al. Binary ethylenimine inactivated Japanese encephalitis virus antigen reveals hemagglutination[J]. Open J Vet Med,2012,2(3):120-123.
[30] TANG L,KANG H,DUAN K,et al. Effects of three types of inactivation agents on the antibody response and immune protection of inactivated IHNV vaccine in rainbow trout[J]. Viral Immunol,2016,29(7):430-435.
[31] TANG W H,JIA S R. Effect of cell membrane permeability on yield of bacteria cellulose produced by acetobacter xylinum[J].Food Engi,2017,42(1):13-16.(in Chinese)汤卫华,贾士儒.细胞膜通透性的调节对木醋杆菌产细菌纤维素的影响[J].食品工程,2017,42(1):13-16.
[32]王庆利,何丹,郑晓冬,等.有机溶剂对细胞的毒害及细胞的耐受性机制[J].微生物学通报,2002,29(4):81-85.
[33] KNIGHT C A,STANLEY W M. The effect of some chemicals on purified influenza virus[J]. J Exp Med,1944,79(3):291-300.
[34]马悦,张元兴.国外鱼类疫苗之路[J].海洋与渔业·水产前沿,2013,9(1):80-83.
[35] LIU X,ZHANG H,GAO Y,et al. Efficacy of chitosan oligosaccharide as aquatic adjuvant administrated with a formalininactivated Vibrio anguillarum,vaccine[J]. Fish Shellfish Immunol,2015,47(2):855-860.
[36]朱洁莲,高婷婷,钟孝俊,等.罗非鱼源无乳链球菌灭活疫苗免疫效果的研究[J].中国兽医科学,2017,47(5):636-643.
[37] NGUYEN H T,THU NGUYEN T T,TSAI M A,et al. A formalin-inactivated vaccine provides good protection against Vibrio harveyi,infection in orange-spotted grouper(Epinephelus coioides)[J]. Fish Shellfish Immunol,2017,65(6):118-126.
[38] ZHANG L,LI Z,ZHANG Q,et al. Efficacy assessment of an inactivated Tembusu virus vaccine candidate in ducks[J]. Res Vet Sci,2017,110(2):72-78.
[39] STAUFFER F,EL. BACHA T,DA POIAN A T. Advances in the development of inactivated virus vaccines[J]. Recent Pat Antiinfect Drug Discov,2006,1(3):291-296.
[40] SUN J B,ZENG L B,ZHANG H,et al. Studies on the method of inactivating giant salamander iridovirus withβ-propiolactone[J]. Freshwater Fisher,2013,43(3):66-71.(in Chinese)孙建滨,曾令兵,张辉,等.大鲵虹彩病毒β-丙内酯灭活方法的研究[J].淡水渔业,2013,43(3):66-71.
[41] ZENG W,WANG Q,WANG Y,et al. Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of grass carp reovirus[J]. Fish Shellfish Immunol,2016,54(7):473-480.
[42] ZHANG L,MA J,FAN Y,et al. Immune response and protection in gibel carp,Carassius gibelio,after vaccination withβ-propiolactone inactivated cyprinid herpesvirus 2[J]. Fish Shellfish Immunol,2016,49(2):344-350.
[43] SARKAR A,TAMIL SELVAN R P,KISHORE S,et al. Comparison of different inactivation methods on the stability of Indian vaccine strains of foot and mouth disease virus[J]. Biologicals,2017,48(1):10-23.
[44] REDDY G S,SRINIVASAN V A. Comparison of two experimental binary ethylenimine(BEI)inactivated Newcastle disease oil. emulsion vaccines[J]. Acta Virol,1991,35(2):196-199.
[45] BITEW M,NANDI S,RAVISHANKAR C,et al. Humoral immune response and protective efficacy of binary ethylenimine(BEI)inactivated pentavalent bluetongue vaccine after challenge with homologous virus in sheep[J]. Int J Virol,2017,13(1):43-52.
[46] YEOM M,LYOO K S,KANG B K,et al. Efficacy of a combined inactivated porcine reproductive and respiratory syndrome virus vaccine using North American and European strains in specific pathogen free pigs[J]. Vet J,2015,204(2):157-161.
[47] GONZ魣LEZ M C,P魪REZ N,BERMUDEZ V. Oil inactivated vaccine development with venezuelan strain of Eastern equine encephalitis virus[J]. Rev Fac Cs Vet,2014,55(2):78-87.
[48] ANDERSON E,CLOUTHIER S,SHEWMAKER W,et al. Inactivated infectious haematopoietic necrosis virus,(IHNV)vaccines[J]. J Fish Dis,2008,31(10):729-745.
[49] KAI Y H,WU Y C,CHI S C. Immune gene expressions in grouper larvae(Epinephelus coioides)induced by bath and oral vaccinations with inactivated betanodavirus[J]. Fish Shellfish Immunol,2014,40(2):563-569.
[50] HUO J Q,ZHAO L M,TAN Y X,et al. Studies on inactivated vaccine of Pseudomonas plecoglossicida[J]. J Jimei Univ(Nat Sci),2017,22(4):12-19.(in Chinese)霍建强,赵玲敏,覃映雪,等.变形假单胞菌灭活疫苗研究[J].集美大学学报(自然科学版),2017,22(4):12-19.
[51] XIAO H,LI J,WANG X H,et al. Preparation of vaccine against vibriosis of sea perch late olabrax japonicas and its immune efficiency evaluation[J]. J Ocean Univ of Qingdao(Nat Sci),2003,33(2):226-232.(in Chinese)肖慧,李军,王祥红,等.鲈鱼鳗弧菌病疫苗的制备及免疫防治效果[J].青岛海洋大学学报(自然科学版),2003,33(2):226-232.
[52]刘堂水,陈昌福,樊海平.不同方法灭活的痢疾志贺氏杆菌菌苗对罗非鱼免疫原性的影响[J].北京水产,2007(2):45-48.
[53] LIU G Y,MAO Z J,CHEN C R,et al. Study on the immunogenicity of Vibrio harveyi bacterins made by different inactivated methods to silver crucian carp Carassius auratus gibelio[J]. J Huazhong Agr Univ,2002,21(2):139-142.(in Chinese)刘国勇,毛芝娟,陈超然,等.不同方法灭活的哈维氏弧菌菌苗对异育银鲫的免疫原性比较[J].华中农业大学学报,2002,21(2):139-142.
[54] KAI Y H,CHI S C. Efficacies of inactivated vaccines against betanodavirus in grouper larvae(Epinephelus coioides)by bath immunization[J]. Vaccine,2008,26(11):1450-1457.
[55] SHI W Z,CHEN C F,JI G L,et al. Immune response of grass carp(Ctenopharyngodon Idellus C. ET V.)to antigens of aeromonas punctata vaccine[J]. J Huazhong Agr Univ,1994,13(5):497-501.(in Chinese)史维舟,陈昌福,纪国良,等.草鱼对点状气单胞菌菌苗的免疫反应[J].华中农业大学学报,1994,13(5):497-501.
[56] LAI Y T. The study of optimal culture conditions and the methods of inactivating infectious spleen and kidney necrosis virus in Siniperca chuatsi[D]. Guangzhou:South China Agricultural University,2016.(in Chinese)赖迎迢.鳜脑细胞系培养条件及传染性脾肾坏死病毒灭活工艺的研究[D].广东广州:华南农业大学,2016.
[57] ZHANG L X. Research on the key techniques of vaccine testing for infectious spleen and kidney necrosis virus of Siniperca chuatsi[J]. Wuhan:Huazhong Agricultural University,2017.(in Chinese)张醴溪.鳜传染性脾肾坏死病毒灭活疫苗检验关键技术研究[D].湖北武汉:华中农业大学,2017.
[58] WILTON T,DUNN G,EASTWOOD D,et al. Effect of formaldehyde inactivation on poliovirus[J]. J Virol,2014,88(20):11955-11964.
[2]农业部渔业渔政管理局. 2016年全国渔业经济统计公报[R/OL].(2017-07-25)[2018-01-20]. http://www.cnfm.gov.cn/yyywyzj/201707/t20170725_5759859.htm.
[3] OU-YANG Z,WANG P,HUANG X,et al. Immunogenicity and protective effects of inactivated Singapore grouper iridovirus(SGIV)vaccines in orange-spotted grouper,Epinephelus coioides[J]. Dev Comp Immunol,2012,38(2):254-261.
[4] WU S Q,TAO J F,GONG H,et al. Situation and tendency of the fishery vaccine development[J]. Chin Fish Qual Stand,2014,4(1):1-13.(in Chinese)吴淑勤,陶家发,巩华,等.渔用疫苗发展现状及趋势[J].中国渔业质量与标准,2014,4(1):1-13.
[5]李超,蒋梦娜,丁兆钧,等.渔用疫苗的研究进展[J].农业开发与装备,2015,11(7):72-72,110.
[6] ZHANG Y,ZHOU L,ZHANG Y. Investigation of UV. TiO2 photocatalysis and its mechanism in Bacillus subtilis spore inactivation[J]. J Environ Sci(China),2014,26(9):1943-1948.
[7] HUSSAIN I,PRICE G W,FARID A H. Inactivation of Aleutian mink disease virus through high temperature exposure in vitro and under field-based composting conditions[J]. Vet Microbiol,2014,173(1-2):50-58.
[8] LI J,SUO Y,LIAO X,et al. Analysis of Staphylococcus aureus cell viability,sublethal injury and death induced by synergistic combination of ultrasound and mild heat[J]. Ultrason Sonochem,2017,39(11):101-110.
[9] BOUDARKOV V A,SEREDA A D,CARPOV O N,et al.Using gamma rays to inactivate African swine fever virus[J].Russian Agri Sci,2016,42(5):375-377.
[10] GERALD O. Some bacteria are not inactivated by ultrasound:here's why[J]. Chem Engi,2013,120(9):16.
[11] HABIB M,HUSSAIN I,FANG W H,et al. Inactivation of infectious bursal disease virus by binary ethylenimine and formalin[J]. J Zhejiang Univ Sci B,2006,7(4):320-323.
[12] MORROW W J W,SHEIKH N A,SCHMIDT C S,et al. Vaccinology:principles and practice[M]. New Jersey:Wiley-Blackwell,2012,44(1. 3):224-236.
[13] JEONG Y U,SUBRAMANIAN D,YEOUNGHWAN J,et al.Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder,Paralichthys olivaceus[J]. Arch Polish Fisher,2016,24(1):23-32.
[14] AMANNA I J,RAUéH P,SLIFKA M K. Development of a new hydrogen peroxide-based vaccine platform[J]. Nat Med,2012,18(6):974-979.
[15] SEO H S. Application of radiation technology in vaccines development[J]. Clin Exp Vac Res,2015,4(2):145-158.
[16] METZ B,KERSTEN G P,HOOGERHOUT P,et al. Identification of formaldehyde-induced modifications in proteins:reactions with model peptides[J]. J Biol Chem,2004,279(8):6235-6243.
[17] SRINIVASA S,DING X,KAST J. Formaldehyde cross-linking and structural proteomics:Bridging the gap[J]. Methods,2015,89(1):91-98.
[18] PAWAR S D,MURTADAK V B,KALE S D,et al. Evaluation of different inactivation methods for high and low pathogenic avian influenza viruses in egg-fluids for antigen preparation[J]. J Virol Methods,2015,222(1):28-33.
[19] GONG W B,HAO W W,LIAO W P,et al. Inactivation effect of genotypeⅦNewcastle virus with two different inactivants[J]. Sichuan Anim Vet Sci,2017,44(10):21-23.(in Chinese)龚文波,郝伟伟,廖韦萍,等.两种不同灭活剂对基因Ⅶ型新城疫病毒灭活效果的影响研究[J].四川畜牧兽医,2017,44(10):21-23.
[20] MA Q R,ZHANG L,YANG X,et al. Progress in research on determination methods for residual formaldehyde content in vaccines[J]. Chin J Biologicals,2017,30(12):1351-1353,1358.(in Chinese)马秋冉,张璐,杨星,等.疫苗中甲醛残留量检测方法的研究进展[J].中国生物制品学杂志,2017,30(12):1351-1353,1358.
[21] H魧NNINE O,KNOL A B,JANTUNEN M,et al. Environmental burden of disease in Europe:assessing nine risk factors in six countries[J]. Environ Health Perspect,2014,122(5):439-446.
[22] NIELSEN G D,LARSEN S T,WOLKOFF P. Re-evaluation of the WHO(2010)formaldehyde indoor air quality guideline for cancer risk assessment[J]. Arch Toxicol,2017,91(1):35-61.
[23] NILSEM H,LILLEHAUG A,TAKSDAL T,et al. Toxicity of intraperitoneally injected formalin in Atlantic salmon,Salmo salar L[J]. J Fish Dis,2010,15(4):323-329.
[24] XU S Z,YIN Y B,WANG X. Research progress on routine inactivants in veterinary vaccines[J]. China Anim Husb Vet Med,2010,37(9):162-167.(in Chinese)徐守振,尹燕博,王新.动物疫苗中常用抗原灭活剂的研究进展[J].中国畜牧兽医,2010,37(9):162-167.
[25] PERRIN P,MORGEAUX S. Inactivation of DNA byβ-propiolactone[J]. Biologicals,1995,23(3):207-211.
[26] DELRUE I,VERZELE D,MADDER A,et al. Inactivated virus vaccines from chemistry to prophylaxis:merits,risks and challenges[J]. Expert Rev Vaccines,2012,11(6):695-719.
[27] JIANG L M,LIN X B,GAO L,et al. Inactivated of rabies virus withβ-propiolactone[J]. Int J Epidemiol Infect Dis,2014,41(2):137-139.(in Chinese)姜立民,林晓波,高磊,等.β.丙内酯对狂犬病病毒的灭活效果[J].国际流行病学传染病学杂志,2014,41(2):137-139.
[28] LIU Z W,LI F A,QIAN H. Study for application ofβ-propiolactone on preparation of human rabies vaccine of purified hamster kidney cell[J]. Chin J Zoon,1999,15(6):71-72.(in Chinese)刘兆文,李福安,钱浩.β-丙内酯在人用纯化地鼠肾细胞狂犬疫苗制备中的应用研究[J].中国人兽共患病学报,1999,15(6):71-72.
[29] YANG D K,HAHYUN K,JINJU N,et al. Binary ethylenimine inactivated Japanese encephalitis virus antigen reveals hemagglutination[J]. Open J Vet Med,2012,2(3):120-123.
[30] TANG L,KANG H,DUAN K,et al. Effects of three types of inactivation agents on the antibody response and immune protection of inactivated IHNV vaccine in rainbow trout[J]. Viral Immunol,2016,29(7):430-435.
[31] TANG W H,JIA S R. Effect of cell membrane permeability on yield of bacteria cellulose produced by acetobacter xylinum[J].Food Engi,2017,42(1):13-16.(in Chinese)汤卫华,贾士儒.细胞膜通透性的调节对木醋杆菌产细菌纤维素的影响[J].食品工程,2017,42(1):13-16.
[32]王庆利,何丹,郑晓冬,等.有机溶剂对细胞的毒害及细胞的耐受性机制[J].微生物学通报,2002,29(4):81-85.
[33] KNIGHT C A,STANLEY W M. The effect of some chemicals on purified influenza virus[J]. J Exp Med,1944,79(3):291-300.
[34]马悦,张元兴.国外鱼类疫苗之路[J].海洋与渔业·水产前沿,2013,9(1):80-83.
[35] LIU X,ZHANG H,GAO Y,et al. Efficacy of chitosan oligosaccharide as aquatic adjuvant administrated with a formalininactivated Vibrio anguillarum,vaccine[J]. Fish Shellfish Immunol,2015,47(2):855-860.
[36]朱洁莲,高婷婷,钟孝俊,等.罗非鱼源无乳链球菌灭活疫苗免疫效果的研究[J].中国兽医科学,2017,47(5):636-643.
[37] NGUYEN H T,THU NGUYEN T T,TSAI M A,et al. A formalin-inactivated vaccine provides good protection against Vibrio harveyi,infection in orange-spotted grouper(Epinephelus coioides)[J]. Fish Shellfish Immunol,2017,65(6):118-126.
[38] ZHANG L,LI Z,ZHANG Q,et al. Efficacy assessment of an inactivated Tembusu virus vaccine candidate in ducks[J]. Res Vet Sci,2017,110(2):72-78.
[39] STAUFFER F,EL. BACHA T,DA POIAN A T. Advances in the development of inactivated virus vaccines[J]. Recent Pat Antiinfect Drug Discov,2006,1(3):291-296.
[40] SUN J B,ZENG L B,ZHANG H,et al. Studies on the method of inactivating giant salamander iridovirus withβ-propiolactone[J]. Freshwater Fisher,2013,43(3):66-71.(in Chinese)孙建滨,曾令兵,张辉,等.大鲵虹彩病毒β-丙内酯灭活方法的研究[J].淡水渔业,2013,43(3):66-71.
[41] ZENG W,WANG Q,WANG Y,et al. Immunogenicity of a cell culture-derived inactivated vaccine against a common virulent isolate of grass carp reovirus[J]. Fish Shellfish Immunol,2016,54(7):473-480.
[42] ZHANG L,MA J,FAN Y,et al. Immune response and protection in gibel carp,Carassius gibelio,after vaccination withβ-propiolactone inactivated cyprinid herpesvirus 2[J]. Fish Shellfish Immunol,2016,49(2):344-350.
[43] SARKAR A,TAMIL SELVAN R P,KISHORE S,et al. Comparison of different inactivation methods on the stability of Indian vaccine strains of foot and mouth disease virus[J]. Biologicals,2017,48(1):10-23.
[44] REDDY G S,SRINIVASAN V A. Comparison of two experimental binary ethylenimine(BEI)inactivated Newcastle disease oil. emulsion vaccines[J]. Acta Virol,1991,35(2):196-199.
[45] BITEW M,NANDI S,RAVISHANKAR C,et al. Humoral immune response and protective efficacy of binary ethylenimine(BEI)inactivated pentavalent bluetongue vaccine after challenge with homologous virus in sheep[J]. Int J Virol,2017,13(1):43-52.
[46] YEOM M,LYOO K S,KANG B K,et al. Efficacy of a combined inactivated porcine reproductive and respiratory syndrome virus vaccine using North American and European strains in specific pathogen free pigs[J]. Vet J,2015,204(2):157-161.
[47] GONZ魣LEZ M C,P魪REZ N,BERMUDEZ V. Oil inactivated vaccine development with venezuelan strain of Eastern equine encephalitis virus[J]. Rev Fac Cs Vet,2014,55(2):78-87.
[48] ANDERSON E,CLOUTHIER S,SHEWMAKER W,et al. Inactivated infectious haematopoietic necrosis virus,(IHNV)vaccines[J]. J Fish Dis,2008,31(10):729-745.
[49] KAI Y H,WU Y C,CHI S C. Immune gene expressions in grouper larvae(Epinephelus coioides)induced by bath and oral vaccinations with inactivated betanodavirus[J]. Fish Shellfish Immunol,2014,40(2):563-569.
[50] HUO J Q,ZHAO L M,TAN Y X,et al. Studies on inactivated vaccine of Pseudomonas plecoglossicida[J]. J Jimei Univ(Nat Sci),2017,22(4):12-19.(in Chinese)霍建强,赵玲敏,覃映雪,等.变形假单胞菌灭活疫苗研究[J].集美大学学报(自然科学版),2017,22(4):12-19.
[51] XIAO H,LI J,WANG X H,et al. Preparation of vaccine against vibriosis of sea perch late olabrax japonicas and its immune efficiency evaluation[J]. J Ocean Univ of Qingdao(Nat Sci),2003,33(2):226-232.(in Chinese)肖慧,李军,王祥红,等.鲈鱼鳗弧菌病疫苗的制备及免疫防治效果[J].青岛海洋大学学报(自然科学版),2003,33(2):226-232.
[52]刘堂水,陈昌福,樊海平.不同方法灭活的痢疾志贺氏杆菌菌苗对罗非鱼免疫原性的影响[J].北京水产,2007(2):45-48.
[53] LIU G Y,MAO Z J,CHEN C R,et al. Study on the immunogenicity of Vibrio harveyi bacterins made by different inactivated methods to silver crucian carp Carassius auratus gibelio[J]. J Huazhong Agr Univ,2002,21(2):139-142.(in Chinese)刘国勇,毛芝娟,陈超然,等.不同方法灭活的哈维氏弧菌菌苗对异育银鲫的免疫原性比较[J].华中农业大学学报,2002,21(2):139-142.
[54] KAI Y H,CHI S C. Efficacies of inactivated vaccines against betanodavirus in grouper larvae(Epinephelus coioides)by bath immunization[J]. Vaccine,2008,26(11):1450-1457.
[55] SHI W Z,CHEN C F,JI G L,et al. Immune response of grass carp(Ctenopharyngodon Idellus C. ET V.)to antigens of aeromonas punctata vaccine[J]. J Huazhong Agr Univ,1994,13(5):497-501.(in Chinese)史维舟,陈昌福,纪国良,等.草鱼对点状气单胞菌菌苗的免疫反应[J].华中农业大学学报,1994,13(5):497-501.
[56] LAI Y T. The study of optimal culture conditions and the methods of inactivating infectious spleen and kidney necrosis virus in Siniperca chuatsi[D]. Guangzhou:South China Agricultural University,2016.(in Chinese)赖迎迢.鳜脑细胞系培养条件及传染性脾肾坏死病毒灭活工艺的研究[D].广东广州:华南农业大学,2016.
[57] ZHANG L X. Research on the key techniques of vaccine testing for infectious spleen and kidney necrosis virus of Siniperca chuatsi[J]. Wuhan:Huazhong Agricultural University,2017.(in Chinese)张醴溪.鳜传染性脾肾坏死病毒灭活疫苗检验关键技术研究[D].湖北武汉:华中农业大学,2017.
[58] WILTON T,DUNN G,EASTWOOD D,et al. Effect of formaldehyde inactivation on poliovirus[J]. J Virol,2014,88(20):11955-11964.