不同干燥方式对桑枝提取物物理指纹图谱及其总黄酮含量的影响
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摘要
目的:考察不同干燥方式对桑枝提取物物理指纹图谱及其总黄酮含量的影响。方法:分别采用常压干燥、减压干燥及冷冻干燥对桑枝浸膏进行干燥,并测定其总黄酮含量,构建以雷达图直观展现的桑枝提取物物理指纹图谱,对3种不同干燥方式所得桑枝提取物物理指纹图谱进行比较,通过可压参数比较不同干燥方式所得桑枝提取物的压缩特性。结果:不同干燥方式所得桑枝提取物对照物理指纹谱相似度77. 8%~87. 3%,一级指标平均值及可压参数的RSD处于0~16. 6%,除均一性及稳定性外,其余RSD均<10%。不同干燥方式对桑枝提取物稳定性及均一性影响较大,对其他因素影响较小。结论:不同干燥方式对桑枝提取物物理指纹图谱有一定的影响,而对桑枝总黄酮含量影响较小。
Objective: To investigate the influence of different drying methods on physical fingerprint and total flavonoids content of Mori Ramulus extract. Method: The Mori Ramulus extract was dried by freeze drying,vacuum drying,ambient pressure drying,respectively. The content of total flavonoids in samples with different drying methods were determined and compared with each other. Physical fingerprint of Mori Ramulus extract was established by radar map and the physical fingerprint of samples with different drying methods were compared with each other. In the meantime,compressible parameters were employed to analyze the compressibility characteristics of Mori Ramulus extract treated by different drying methods. Result: Similarity of control physical fingerprint of Mori Ramulus extract treated by different drying methods was 77. 8%-87. 3%,relative standard deviation( RSD)of average value of the first level indexes and the compressible parameters was 0-16. 6%. Except for homogeneity and stability,the remaining RSDs were less than 10%. Effects of different drying methods on homogeneity and stability of Mori Ramulus extract were greater than that of other factors. Conclusion: Different drying methods have certain effects on physical fingerprint of Mori Ramulus extract,but they have little influence on total flavonoids content in Mori Ramulus extract.
Objective: To investigate the influence of different drying methods on physical fingerprint and total flavonoids content of Mori Ramulus extract. Method: The Mori Ramulus extract was dried by freeze drying,vacuum drying,ambient pressure drying,respectively. The content of total flavonoids in samples with different drying methods were determined and compared with each other. Physical fingerprint of Mori Ramulus extract was established by radar map and the physical fingerprint of samples with different drying methods were compared with each other. In the meantime,compressible parameters were employed to analyze the compressibility characteristics of Mori Ramulus extract treated by different drying methods. Result: Similarity of control physical fingerprint of Mori Ramulus extract treated by different drying methods was 77. 8%-87. 3%,relative standard deviation( RSD)of average value of the first level indexes and the compressible parameters was 0-16. 6%. Except for homogeneity and stability,the remaining RSDs were less than 10%. Effects of different drying methods on homogeneity and stability of Mori Ramulus extract were greater than that of other factors. Conclusion: Different drying methods have certain effects on physical fingerprint of Mori Ramulus extract,but they have little influence on total flavonoids content in Mori Ramulus extract.
引文
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[16]伍蕊嗣,刘涛,覃盼盼,等.热毒宁注射液物理指纹谱研究及应用[J].中国中药杂志,2017,42(3):505-509.
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[18]陈绪龙,赵国巍,廖正根,等.当归超微粉体和普通粉体的粉体学性质比较[J].中国实验方剂学杂志,2010,16(18):1-5.
[2]刘刚,佟万红,黄盖群,等.桑枝的营养功能性成分及我国桑枝综合利用研究[J].陕西农业科学,2008,54(6):95-98.
[3]郭福团,许雄伟,潘建峰,等.桑枝多糖对糖尿病肾病小鼠肾皮质氧化应激作用的影响[J].中国药理学通报,2016,32(8):1148-1152.
[4]张作,金洁,时连,等.反相高效液相色谱法测定桑枝中1-脱氧野尻霉素的含量[J].中国药学杂志,2007,42(7):535-538.
[5]黄勇,张林,刘利,等.桑枝资源中总黄酮类化合物的研究进展[J].蚕桑通报,2006,37(4):56-57.
[6]杨胤,冯怡,徐德生,等.干燥工艺与中药提取物物理性质的相关性研究[J].中国药学杂志,2008,43(17):1295-1299.
[7]李会伟,刘培,钱大玮,等.不同干燥方法及其影响因子对玄参药材初加工过程品质形成的影响[J].中国中药杂志,2015,40(22):4417-4423.
[8]冯怡,洪燕龙,鲜洁晨,等.基于Qb D理念的中药新药成型工艺研发模式的探讨[J].中国中药杂志,2014,39(17):3404-3408.
[9]张萍,付建华,梁新丽,等.不同干燥方式对中药提取物粉体学性质的影响[J].江西医药,2014,49(12):1394-1396.
[10]梁新丽,张萍,廖正根,等.干燥方式对葛根提取物药剂学性质的影响[J].中国药学杂志,2010,45(10):756-760.
[11]徐玉玲,谢敏,梁悦,等.基于Qb D理念优选川明参口含片的制备工艺[J].中国实验方剂学杂志,2018,24(17):14-19.
[12]张毅,徐冰,孙飞,等.中药提取物粉末物理指纹谱研究及应用[J].中国中药杂志,2016,41(12):2221-2227.
[13]徐芳琴,张新睿,郭凤伟,等.中成药质量控制研究进展[J].现代生物医学进展,2014,14(31):6159-6163.
[14]梁小银,陈少旭,吴垠.中药制剂质量控制研究的发展趋势[J].中国药房,2014,25(3):280-283.
[15]刘莹,封亮,贾晓斌.中药制剂质量的影响因素探析[J].中国中药杂志,2017,42(9):1808-1813.
[16]伍蕊嗣,刘涛,覃盼盼,等.热毒宁注射液物理指纹谱研究及应用[J].中国中药杂志,2017,42(3):505-509.
[17]刘珈羽,郭换,肖佳雯,等.不同粒径白及粉的粉体学性质及体外溶出度比较[J].中国实验方剂学杂志,2018,24(3):25-29.
[18]陈绪龙,赵国巍,廖正根,等.当归超微粉体和普通粉体的粉体学性质比较[J].中国实验方剂学杂志,2010,16(18):1-5.