微RNA与急性心肌梗死关系的研究进展
|
摘要
微RNA(miRNA)可调节转录后基因的表达。血浆微RNA的表达在急性心肌梗死患者中显著升高,标志着miRNA在心肌梗死的病理生理机制中发挥重要作用,其中miR-1的过表达可促进心肌梗死后心肌细胞凋亡;miR-126的表达对心肌细胞凋亡有抑制作用; miR-214的高表达对过氧化氢诱导的心肌细胞凋亡具有保护作用。这些研究为治疗心血管疾病提供了新的思路。然而,miRNA作为急性心肌梗死的诊断和预后的生物标志物在应用于临床之前还有诸多问题未能解决,仍需进行大规模深入和系统的研究。
MicroRNAs( miRNAs) regulate the expression of post-transcriptional genes. The expression of plasma microRNA is significantly increased in patients with acute myocardial infarction,which indicates that miRNA plays an important role in the pathophysiological mechanism of myocardial infarction. The overexpression of miR-1 may promote cardiomyocyte apoptosis after myocardial infarction; miR-126 expression has an inhibitory effect on cardiomyocyte apoptosis;high expression of miR-214 has protective effect on hydrogen peroxide-induced cardiomyocyte apoptosis. These studies provide new ideas for the treatment of cardiovascular diseases. However,there are still many problems not solved for miRNAs to be used as biomarkers for the diagnosis and prognosis of acute myocardial infarction,and large-scale in-depth and systematic research is still needed.
MicroRNAs( miRNAs) regulate the expression of post-transcriptional genes. The expression of plasma microRNA is significantly increased in patients with acute myocardial infarction,which indicates that miRNA plays an important role in the pathophysiological mechanism of myocardial infarction. The overexpression of miR-1 may promote cardiomyocyte apoptosis after myocardial infarction; miR-126 expression has an inhibitory effect on cardiomyocyte apoptosis;high expression of miR-214 has protective effect on hydrogen peroxide-induced cardiomyocyte apoptosis. These studies provide new ideas for the treatment of cardiovascular diseases. However,there are still many problems not solved for miRNAs to be used as biomarkers for the diagnosis and prognosis of acute myocardial infarction,and large-scale in-depth and systematic research is still needed.
引文
[1]Nabiaek E,Wańha W,Kula D,et al.Circulating microRNAs(miR-423-5p,miR-208a and miR-1)in acute myocardial infarction and stable coronary heart disease[J].Minerva Cardioangiol,2013,61(6):627-637.
[2]Szabo L,Morey R,Palpant NJ,et al.Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development[J].Genome Biol,2015,16:126.
[3]Reid G,Kirschner MB,Van zandwijk N.Circulating microRNAs:Association with disease and potential use as biomarkers[J].Crit Rev Oncol Hematol,2011,80(2):193-208.
[4]Cheng Y,Wang X,Yang J,et al.A translational study of urine miRNAs in acute myocardial infarction[J].J Mol Cell Cardiol,2012,53(5):668-676.
[5]Ji X,Takahashi R,Hiura Y,et al.Plasma miR-208 as a biomarker of myocardial injury[J].Clin Chem,2009,55(11):1944-1949.
[6]Zhu J,Yao K,Wang Q,et al.Ischemic Postconditioning-Regulated miR-499 Protects the Rat Heart Against Ischemia/Reperfusion Injury by Inhibiting Apoptosis through PDCD4[J].Cell Physiol Biochem,2016,39(6):2364-2380.
[7]Duan L,Xiong X,Liu Y,et al.miRNA-1:Functional roles and dysregulation in heart disease[J].Mol Biosyst,2014,10(11):2775-2782.
[8]Shan ZX,Lin QX,Deng CY,et al.miR-1/miR-206 regulate Hsp60 expression contributing to glucose-mediated apoptosis in cardiomyocytes[J].FEBS Lett,2010,584(16):3592-3600.
[9]Ai J,Zhang R,Li Y,et al.Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction[J].Biochem Biophys Res Commun,2010,391(1):73-77.
[10]段晓霞,周贤,王晓斌,等.心肌组织特异性微小RNA-1在ST段抬高型急性心肌梗死患者尿中含量的变化研究[J].中国危重病急救医学,2012,24(12):709-712.
[11]肖祥,胡建华.miRNA-1和急性心肌梗死后心肌缺血程度的相关性研究[J].中外医疗,2015,34(18):3-5.
[12]Mondadori dos santos A,Metzinger L,Haddad O,et al.miR-126is involved in vascular remodeling under laminar shear stress[J].Biomed Res Int,2015,2015:497280.
[13]Gabriele T,Trombetta A,Patrizia D,et al.Unacylated ghrelin induces oxidative stress resistance in a glucose intolerance and peripheral artery disease mouse model by restoring endothelial cell miR-126 expression[J].Diabetes,2015,64(4):1370-1382.
[14]Wang JN,Yan YY,Guo ZY,et al.Negative association of circulating microRNA-126 with high-sensitive C-reactive protein and vascular cell adhesion molecule-1 in patients with coronary artery disease following percutaneous coronary intervention[J].Chin Med J(Engl),2016,129(23):2786-2791.
[15]Ye PP,Liu J,He FY,et al.Hypoxia-induced deregulation of miR-126and its regulative effect on VEGF and MMP-9 expression[J].Int J Med Sci,2014,11(1):17-23.
[16]高威,钟锋.miR-126对急性心肌梗死大鼠心肌细胞凋亡的影响[J].中国比较医学杂志,2016,26(7):57-63.
[17]吴雪纯,贾永平.冠状动脉粥样硬化性心脏病血浆微小RNA-126的表达水平及其临床意义[J].国际心血管病杂志,2017(6):365-368.
[18]江哲龙,王东升,何爱文,等.心肺复苏后大鼠血浆中微小核糖核酸在心肌损伤中的变化及其意义[J/CD].中华危重症医学杂志(电子版),2017,44(5):316-321.
[19]王晶,韩雪晶,贾克刚,等.循环microRNA-126作为急性心肌梗死早期诊断和预后生物标志物的研究[J/CD].中华临床医师杂志(电子版),2015,9(3):409-414.
[20]Widera C,Gupta SK,Lorenzen JM,et al.Diagnostic and prognostic impact of six circulating microRNAs in acute coronary syndrome[J].J Mol Cell Cardiol,2011,51(5):872-875.
[21]Deddens JC,Colijn JM,Oerlemans MI,et al.Circulating microRNAs as novel biomarkers for the early diagnosis of acute coronary syndrome[J].J Cardiovasc Transl Res,2013,6(6):884-898.
[22]卢章,朱继荣,余小平.微小核糖核苷酸对急性心肌梗死的早期诊断价值研究[J].岭南急诊医学杂志,2015,20(4):271-272.
[23]Duan Q,Yang L,Gong W,et al.MicroRNA-214 Is Upregulated in Heart Failure Patients and Suppresses XBP1-Mediated Endothelial Cells Angiogenesis[J].J Cell Physiol,2015,230(8):1964-1973.
[24]肖亚利,赵敏,吕平,等.血浆microRNA-214在AMI患者中的表达及其与左室重构的关系[J].天津医药,2017,45(5):501-505.
[25]Yang X,Qin Y,Shao S,et al.MicroRNA-214 inhibits left ventricular remodeling in an acute myocardial infarction rat model by suppressing cellular apoptosis via the phosphatase and tensin homolog(PTEN)[J].Int Heart J,2016,57(2):247-250.
[26]符雅菁,金艳.血浆微小RNA-214在冠状动脉疾病患者中的表达及临床意义[J].临床与病理杂志,2018,38(1):28-34.
[27]Wang JX,Jiao JQ,Li Q,et al.miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1[J].Nat Med,2011,17(1):71-78.
[28]Hosoda T,Zheng H,Cabral-da-silva M,et al.Human cardiac stem cell differentiation is regulated by a mircrine mechanism[J].Circulation,2011,123(12):1287-1296.
[29]Wang J,Jia Z,Zhang C,et al.miR-499 protects cardiomyocytes from H2O2-induced apoptosis via its effects on Pdcd4 and Pacs2[J].RNABiol,2014,11(4):339-350.
[30]肖蕾,沈飞燕,洪斌,等.血液微小RNA-499对早期诊断急性心肌梗死价值的Meta分析[J].安徽医药,2018,22(1):50-54.
[31]许丽霞,张丽科,杨云山,等.微小RNA-499对STEMI的早期诊断价值[J].检验医学与临床,2017,14(21):3191-3192.
[2]Szabo L,Morey R,Palpant NJ,et al.Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development[J].Genome Biol,2015,16:126.
[3]Reid G,Kirschner MB,Van zandwijk N.Circulating microRNAs:Association with disease and potential use as biomarkers[J].Crit Rev Oncol Hematol,2011,80(2):193-208.
[4]Cheng Y,Wang X,Yang J,et al.A translational study of urine miRNAs in acute myocardial infarction[J].J Mol Cell Cardiol,2012,53(5):668-676.
[5]Ji X,Takahashi R,Hiura Y,et al.Plasma miR-208 as a biomarker of myocardial injury[J].Clin Chem,2009,55(11):1944-1949.
[6]Zhu J,Yao K,Wang Q,et al.Ischemic Postconditioning-Regulated miR-499 Protects the Rat Heart Against Ischemia/Reperfusion Injury by Inhibiting Apoptosis through PDCD4[J].Cell Physiol Biochem,2016,39(6):2364-2380.
[7]Duan L,Xiong X,Liu Y,et al.miRNA-1:Functional roles and dysregulation in heart disease[J].Mol Biosyst,2014,10(11):2775-2782.
[8]Shan ZX,Lin QX,Deng CY,et al.miR-1/miR-206 regulate Hsp60 expression contributing to glucose-mediated apoptosis in cardiomyocytes[J].FEBS Lett,2010,584(16):3592-3600.
[9]Ai J,Zhang R,Li Y,et al.Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction[J].Biochem Biophys Res Commun,2010,391(1):73-77.
[10]段晓霞,周贤,王晓斌,等.心肌组织特异性微小RNA-1在ST段抬高型急性心肌梗死患者尿中含量的变化研究[J].中国危重病急救医学,2012,24(12):709-712.
[11]肖祥,胡建华.miRNA-1和急性心肌梗死后心肌缺血程度的相关性研究[J].中外医疗,2015,34(18):3-5.
[12]Mondadori dos santos A,Metzinger L,Haddad O,et al.miR-126is involved in vascular remodeling under laminar shear stress[J].Biomed Res Int,2015,2015:497280.
[13]Gabriele T,Trombetta A,Patrizia D,et al.Unacylated ghrelin induces oxidative stress resistance in a glucose intolerance and peripheral artery disease mouse model by restoring endothelial cell miR-126 expression[J].Diabetes,2015,64(4):1370-1382.
[14]Wang JN,Yan YY,Guo ZY,et al.Negative association of circulating microRNA-126 with high-sensitive C-reactive protein and vascular cell adhesion molecule-1 in patients with coronary artery disease following percutaneous coronary intervention[J].Chin Med J(Engl),2016,129(23):2786-2791.
[15]Ye PP,Liu J,He FY,et al.Hypoxia-induced deregulation of miR-126and its regulative effect on VEGF and MMP-9 expression[J].Int J Med Sci,2014,11(1):17-23.
[16]高威,钟锋.miR-126对急性心肌梗死大鼠心肌细胞凋亡的影响[J].中国比较医学杂志,2016,26(7):57-63.
[17]吴雪纯,贾永平.冠状动脉粥样硬化性心脏病血浆微小RNA-126的表达水平及其临床意义[J].国际心血管病杂志,2017(6):365-368.
[18]江哲龙,王东升,何爱文,等.心肺复苏后大鼠血浆中微小核糖核酸在心肌损伤中的变化及其意义[J/CD].中华危重症医学杂志(电子版),2017,44(5):316-321.
[19]王晶,韩雪晶,贾克刚,等.循环microRNA-126作为急性心肌梗死早期诊断和预后生物标志物的研究[J/CD].中华临床医师杂志(电子版),2015,9(3):409-414.
[20]Widera C,Gupta SK,Lorenzen JM,et al.Diagnostic and prognostic impact of six circulating microRNAs in acute coronary syndrome[J].J Mol Cell Cardiol,2011,51(5):872-875.
[21]Deddens JC,Colijn JM,Oerlemans MI,et al.Circulating microRNAs as novel biomarkers for the early diagnosis of acute coronary syndrome[J].J Cardiovasc Transl Res,2013,6(6):884-898.
[22]卢章,朱继荣,余小平.微小核糖核苷酸对急性心肌梗死的早期诊断价值研究[J].岭南急诊医学杂志,2015,20(4):271-272.
[23]Duan Q,Yang L,Gong W,et al.MicroRNA-214 Is Upregulated in Heart Failure Patients and Suppresses XBP1-Mediated Endothelial Cells Angiogenesis[J].J Cell Physiol,2015,230(8):1964-1973.
[24]肖亚利,赵敏,吕平,等.血浆microRNA-214在AMI患者中的表达及其与左室重构的关系[J].天津医药,2017,45(5):501-505.
[25]Yang X,Qin Y,Shao S,et al.MicroRNA-214 inhibits left ventricular remodeling in an acute myocardial infarction rat model by suppressing cellular apoptosis via the phosphatase and tensin homolog(PTEN)[J].Int Heart J,2016,57(2):247-250.
[26]符雅菁,金艳.血浆微小RNA-214在冠状动脉疾病患者中的表达及临床意义[J].临床与病理杂志,2018,38(1):28-34.
[27]Wang JX,Jiao JQ,Li Q,et al.miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1[J].Nat Med,2011,17(1):71-78.
[28]Hosoda T,Zheng H,Cabral-da-silva M,et al.Human cardiac stem cell differentiation is regulated by a mircrine mechanism[J].Circulation,2011,123(12):1287-1296.
[29]Wang J,Jia Z,Zhang C,et al.miR-499 protects cardiomyocytes from H2O2-induced apoptosis via its effects on Pdcd4 and Pacs2[J].RNABiol,2014,11(4):339-350.
[30]肖蕾,沈飞燕,洪斌,等.血液微小RNA-499对早期诊断急性心肌梗死价值的Meta分析[J].安徽医药,2018,22(1):50-54.
[31]许丽霞,张丽科,杨云山,等.微小RNA-499对STEMI的早期诊断价值[J].检验医学与临床,2017,14(21):3191-3192.