辣蓼黄酮分离提取及3种黄酮类组分定量分析
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摘要
目的:优化辣蓼黄酮的分离提取工艺以及定量分析其中3种黄酮类组分。方法:采用水煎煮、醇提、醇提后有机溶剂萃取、醇提及有机溶剂萃取后大孔树脂吸附共4种提取方法分离提取辣蓼黄酮,以紫外分光光度法测定总黄酮含量,通过高效液相色谱法测定辣蓼黄酮中3种黄酮类组分(芦丁、金丝桃苷和槲皮苷)的含量。结果:上述4种分离提取方法所得辣蓼总黄酮含量在1.33%～4.92%,所得总黄酮提取物中芦丁含量在0.1155～0.3648 mg/mL,金丝桃苷含量在0.0055～0.0140 mg/mL,槲皮苷含量在0.0141～0.6613 mg/mL。结论:不同提取工艺对总黄酮得率不同,对3种黄酮类组分的提取效果也有差异。比较试验中所用4种方法,醇提法对辣蓼总黄酮以及辣蓼黄酮中的芦丁、金丝桃苷分离提取效果最理想,而对槲皮苷分离提取效果最优的方法则为水煎煮法。
Objective:To optimize the separation and extraction process of total flavonoids from Polygonum hydropiper L. and quantitative analysis of three flavonoids. Methods:Separation and extraction of PoLygonum hydropiper L. by water decoction,alcohol extraction,organic solvent extraction after alcohol extraction,extraction with macroporous resin after extraction with alcohol mentioned organic solvent. The content of total flavonoids was determined by ultraviolet spectrophotometry. The content of three flavonoids(rutin,hyperoside and quercetin)was determined by HPLC. Results:The content of total flavonoids of PoLygonum hydropiper L. was 1.33%-4.92% in above four extraction methods. The content range of rutin was 0.1155-0.3648 mg/mL,the content range of hyperoside was 0.0055-0.0140 mg/mL,and the content range of quercitrin was 0.0141-0.6613 mg/mL. Conclusion:The yield of total flavonoids is different with different methods,and the extraction effects of rutin,quercitrin and hyperin are also different. Compared with the four methods used in the experiment,the ethanolic extraction is the most ideal method for the extraction of total flavonoids,rutin and hyperin,while the best method for extracting quercetin is the method of water extraction.
Objective:To optimize the separation and extraction process of total flavonoids from Polygonum hydropiper L. and quantitative analysis of three flavonoids. Methods:Separation and extraction of PoLygonum hydropiper L. by water decoction,alcohol extraction,organic solvent extraction after alcohol extraction,extraction with macroporous resin after extraction with alcohol mentioned organic solvent. The content of total flavonoids was determined by ultraviolet spectrophotometry. The content of three flavonoids(rutin,hyperoside and quercetin)was determined by HPLC. Results:The content of total flavonoids of PoLygonum hydropiper L. was 1.33%-4.92% in above four extraction methods. The content range of rutin was 0.1155-0.3648 mg/mL,the content range of hyperoside was 0.0055-0.0140 mg/mL,and the content range of quercitrin was 0.0141-0.6613 mg/mL. Conclusion:The yield of total flavonoids is different with different methods,and the extraction effects of rutin,quercitrin and hyperin are also different. Compared with the four methods used in the experiment,the ethanolic extraction is the most ideal method for the extraction of total flavonoids,rutin and hyperin,while the best method for extracting quercetin is the method of water extraction.
引文
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[18]黄新球,杨有仙,梁铖,等.十一种蜂花粉中总黄酮和总多酚含量分析[J].蜜蜂杂志,2017,37(11):3.
[19]吴梅青,李俊雅,陈丹.柑橘皮中总黄酮提取工艺及降血糖活性的试验研究[J].食品研究与开发,2018(5):56.
[20]刘爱朋,焦倩,郑玉光,等.皂角刺总黄酮提取工艺的优化及其抗氧化活性[J].中成药,2018(3):722.
[21]郑敏.正交试验设计优化蓝刺头总黄酮提取工艺[J].中国药业,2018(3):24.
[2]何立美.辣蓼与牛耳枫中黄酮类成分的提取及发酵工艺优化研究[D].广州:广东药学院,2015.
[3]张国英,曾韬.辣蓼主要化学成分的研究[J].林产化学与工业,2005,25(3):21.
[4]YANGX,WANGBC,ZHANGX,etal.Simultaneous determination of nine flavonoids in Polygonum hydropiper L.samples using nanomagnetic powder three-phase hollow fibrebased liquid-phase microextraction combined with ultrahigh performance liquid chromatography-mass spectrometry[J].Journal of Pharmaceutical&Biomedical Analysis,2011,54(2):311.
[5]王峥,曾凡荣,舒俊翔,等.响应面分析法优化金槐总黄酮的提取工艺[J].广州化工,2017,45(20):82.
[6]山光强,陈志刚,许广人,等.金樱子总黄酮的含量测定及酶提工艺优化[J].中国畜牧兽医,2016,43(12):3156.
[7]侯巧芝,信建豪,刘子敬.菠萝皮总黄酮和多糖双水相同时提取的研究[J].食品研究与开发,2018(2):72.
[8]秦凯,刘永震.不同溶剂艾蒿提取液对黄瓜枯萎病菌的抑菌活性研究[J].现代农业科技,2018(1):107.
[9]郝倩.菠萝蜜叶黄酮类化合物的提取及其浸膏的应用[D].北京:北京林业大学,2014.
[10]冯小强,李小芳,杨声,等.桔皮中黄酮类物质提取工艺优化及抗氧化活性的研究[J].湖南农业科学,2012(21):86.
[11]列西·马那甫,力吾汗·阿米汗,景伟文.甘草籽黄酮、多糖复合提取工艺参数优化及抗氧化性研究[J].药物分析杂志,2014(4):686.
[12]张晶,黄士文,徐绍清,等.辣蓼总黄酮超声波辅助提取条件优化研究[J].中国野生植物资源,2016,35(5):25.
[13]叶荣科,张德志,周宏兵,等.小叶榕树叶总黄酮水提醇沉工艺研究[J].广东药学院学报,2003,19(4):330.
[14]王卓慧,张沐新,杨晓虹,等.洋葱中总黄酮乙醇提取工艺的研究[J].中草药,2006,37(3):387.
[15]贾冬英,乔云兰.用正交法探讨苦荞麦茎及籽壳中总黄酮(芦丁)的乙醇提取工艺[J].食品科学,1997,18(6):22-25.
[16]曹艳萍.苦荞麦麸皮中总黄酮的乙醇提取工艺研究[J].食品科学,2005,26(3):98.
[17]毕洁,杨庆利,于丽娜,等.花生壳黄酮乙醇提取工艺探讨[J].现代化工,2008,28(s2):316.
[18]黄新球,杨有仙,梁铖,等.十一种蜂花粉中总黄酮和总多酚含量分析[J].蜜蜂杂志,2017,37(11):3.
[19]吴梅青,李俊雅,陈丹.柑橘皮中总黄酮提取工艺及降血糖活性的试验研究[J].食品研究与开发,2018(5):56.
[20]刘爱朋,焦倩,郑玉光,等.皂角刺总黄酮提取工艺的优化及其抗氧化活性[J].中成药,2018(3):722.
[21]郑敏.正交试验设计优化蓝刺头总黄酮提取工艺[J].中国药业,2018(3):24.