多枝雾水葛化学成分研究
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摘要
目的研究多枝雾水葛Pouzolzia zeylanica var. microphylla醋酸乙酯部位的化学成分。方法采用95%乙醇回流提取,系统溶剂法对醇提物进行萃取,醋酸乙酯萃取部位通过正相、反相柱色谱,半制备高效液相色谱等方法进行分离,化合物通过波谱解析法确定结构。结果从多枝雾水葛醋酸乙酯部位分离鉴定了10个化合物,分别为(2R,3S,4R,5S)-2,5-二-(4-羟基-3-甲氧基苯基)-3,4-二-(3,5-二羟基苯基)四氢呋喃(1)、pouzolignan K(2)、saropeptide(3)、去氢吐叶醇(4)、对羟基苯甲酸(5)、异香兰酸(6)、原儿茶酸(7)、丁香酸(8)、原儿茶醛(9)、原儿茶酸乙酯(10)。结论化合物1为新化合物,命名为异雾水葛木脂素K,化合物3～10均为首次从该属植物中分离得到。
Objective To study the chemical constituents of Pouzolzia zeylanica var. microphylla. Methods 95% ethanol was used for reflux extraction. The solvent extraction method was used to extract the alcohol extracts. The ethyl acetate extracts were separated by normal phase and reversed phase column chromatography and semi-preparative high performance liquid chromatography. The compounds were determined by spectral analysis. Results Ten compounds were isolated and identified from the ethyl acetate part of P. zeylanicaas, which were(2 R,3 S,4 R,5 S)-2,5-bis(4-hydroxy-3-methoxyphenyl)-3,4-bis(3,5-dihydroxybenzene) terahydrofyran(1),pouzolignan K(2), saropeptide(3), dehydrocuciferol(4), p-hydroxybenzoic acid(5), isovanetic acid(6), protocatechuic acid(7),syringic acid(8), protocatechuic aldehyde(9), and ethyl protocatechuate(10), respectively. Conclusion Compound 1 is a new compound named isopouzolignan K, and compounds 3—10 are isolated from this genus for the first time.
Objective To study the chemical constituents of Pouzolzia zeylanica var. microphylla. Methods 95% ethanol was used for reflux extraction. The solvent extraction method was used to extract the alcohol extracts. The ethyl acetate extracts were separated by normal phase and reversed phase column chromatography and semi-preparative high performance liquid chromatography. The compounds were determined by spectral analysis. Results Ten compounds were isolated and identified from the ethyl acetate part of P. zeylanicaas, which were(2 R,3 S,4 R,5 S)-2,5-bis(4-hydroxy-3-methoxyphenyl)-3,4-bis(3,5-dihydroxybenzene) terahydrofyran(1),pouzolignan K(2), saropeptide(3), dehydrocuciferol(4), p-hydroxybenzoic acid(5), isovanetic acid(6), protocatechuic acid(7),syringic acid(8), protocatechuic aldehyde(9), and ethyl protocatechuate(10), respectively. Conclusion Compound 1 is a new compound named isopouzolignan K, and compounds 3—10 are isolated from this genus for the first time.
引文
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[2]Li P,Huo L,Su W,et al.Free radical-scavenging capacity,antioxidant activity and phenolic content of Pouzolzia zeylanica[J].J Serb Chem Soc,2011,76(5):709-717.
[3]陈艳芬,李开莹,邓琪,等.多枝雾水葛对大鼠皮肤溃疡的作用及机制研究[J].中药新药与临床药理,2013,24(3):247-251.
[4]李开莹,陆海玲,陆幸妍,等.多枝雾水葛木脂素类化合物体外抗炎作用及其机制[J].中国药理学与毒理学杂志,2014,28(5):731-736.
[5]刘旭阳,谢郁峰,张慧,等.多枝雾水葛化学成分(I)[J].中国实验方剂学杂志,2014,20(6):43-47.
[6]罗钊,钟楚倩,刘旭阳,等.多枝雾水葛化学成分研究(II)[J].广东药学院报,2014,12(4):430-434.
[7]钟楚倩.多枝雾水葛化学成分研究[D].广州:广东药科大学,2016.
[8]Chen Z H,Zhang H,Tao S H.Norlignans from Pouzolzia zeylanica var.microphylla and their nitric oxide inhibitory activity[J].J Asian Nat Prods Res,2015,17(10):959-966.
[9]李志慧,陈先枚,陶曙红,等.多枝雾水葛化学成分研究(III)[J].广东药科大学学报,2017,33(2):177-180.
[10]Hong F J,Low Y Y,Chong K W,et al.Biomimetic oxidative dimerization of anodically generated stilbene radical cations:effect of aromatic substitution on product distribution and reaction pathways[J].J Org Chem,2014,79(10):4528-4543.
[11]Zhong C Q,Tao S H,Yi Z B.Four new compounds from Pouzol ziazeylanica(L.)Benn.var.microphylla[J].Heterocycles,2015,91(10):1926-1936.
[12]Nwodo N J,Okoye F B C,Lai D W,et al.Two trypanocidal dipeptides from the roots of Zapoteca portoricensis(Fabaceae)[J].Molecules,2014,19:5470-5477.
[13]Kai H,Baba M,Okuyama T.Two new megastigmanes from the leaves of Cucumis sativus[J].Chem Pharm Bull,2007,55(1):133-136.
[14]林建斌,赵立春,郭建忠,等.金荞麦地上部分化学成分的研究[J].中草药,2016,47(11):1841-1844.
[15]岳苏,马齿苋酚性成分的分离及马齿苋酰胺E的药代动力学研究[D].济南:山东大学,2016.
[16]王暐,杨崇仁,张颖君,等.草果果实中的酚类成分[J].云南植物研究,2009,31(3):284-288.
[17]左月明,张忠立,杨雅琴,等.栀子化学成分研究[J].中药材,2013,36(2):225-227.
[18]阎新佳,项峥,温静,等.中药连翘的酚酸类化学成分研究[J].中国药学杂志,2017,52(2):105-108.
[19]张翠,刘占云,於洪建,等.黑豆种皮的酚酸类成分研究[J].中草药,2013,44(24):3440-3443.