不同浓度间歇性低氧时Nrf2的表达及其抗氧化能力
摘要
探讨不同浓度间歇性低氧时Nrf2的表达,研究其低氧应激下的抗氧化能力,利用分光光度计测定血清中低氧应激前、后超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽还原酶(GR)活性及丙二醛(MDA)含量。结果显示肺组织Nrf2阳性颗粒表达,低氧组Nrf2染色阳性颗粒显著多于常氧组。在常氧和5%低氧组,雌雄间超氧化物歧化酶(SOD)活性无显著性差异,10%低氧组,雌性超氧化物歧化酶(SOD)活性显著高于雄性(0.01
引文
[1]吴冒竹,孙树岩,于博,等.鼻内镜手术并发症及相关因素分析.临床耳鼻喉科杂志.2002,16(8):395-396.
[2]周其全,王静,汪云利.氧自由基在高原暴露下血脑屏障通透性改变中的作用及其与高原脑水肿的关系[J].中国微循环,2007,51(3):92-95.
[3]Liu Z,xiang Y,Sun G.The KCTD family of proteins structure, function,and disease rele vance[J].cell Biosci,2013,3(1):45-49.
[4]Lo S C,Li X,Henzl M T,et al.Structure of the keapl Nrf2interface provides mechanistic insight into Nrf2 signaling[J].EMBOJ,2006,25(15):3605-36017.
[5]Miyata t,Takizawa S,van Ypersele de Strihou C.Hypoxia 1Intracellular Sensors for oxygen and oxidative stress novel therapeutic targets.Am J Physiol cell Physiol,2011,300:C226-C231.
[6]Kobayashi A,Kang MI,Watai Y,et al.Oxidative and electrophilic stresses activate Nrf2 through inhibition of ubiquitination activity of keap1[J].Mol cell Biol,2006,26(1):221-229.
[7]李培,方允中.活性氧对蛋白质的损伤作用.《生命的化学》,1994,14(6).
[8]黄丽英,翁锡全,林文,潘海燕,黄利军.间歇低氧训练对抗氧化酶系统及其适应能力的影响.广州体育学院学报,2007,27(2).
[2]周其全,王静,汪云利.氧自由基在高原暴露下血脑屏障通透性改变中的作用及其与高原脑水肿的关系[J].中国微循环,2007,51(3):92-95.
[3]Liu Z,xiang Y,Sun G.The KCTD family of proteins structure, function,and disease rele vance[J].cell Biosci,2013,3(1):45-49.
[4]Lo S C,Li X,Henzl M T,et al.Structure of the keapl Nrf2interface provides mechanistic insight into Nrf2 signaling[J].EMBOJ,2006,25(15):3605-36017.
[5]Miyata t,Takizawa S,van Ypersele de Strihou C.Hypoxia 1Intracellular Sensors for oxygen and oxidative stress novel therapeutic targets.Am J Physiol cell Physiol,2011,300:C226-C231.
[6]Kobayashi A,Kang MI,Watai Y,et al.Oxidative and electrophilic stresses activate Nrf2 through inhibition of ubiquitination activity of keap1[J].Mol cell Biol,2006,26(1):221-229.
[7]李培,方允中.活性氧对蛋白质的损伤作用.《生命的化学》,1994,14(6).
[8]黄丽英,翁锡全,林文,潘海燕,黄利军.间歇低氧训练对抗氧化酶系统及其适应能力的影响.广州体育学院学报,2007,27(2).