基于双层分子印迹及多壁碳纳米管的塞克硝唑碳糊电极的制备及分析应用
|
摘要
为了快速检测药物分子塞克硝唑(Secnidazole,SCZ)的含量,构建了基于多壁碳纳米管和硼嵌入双层分子印迹复合膜的碳糊电极电化学传感器。对影响该传感器性能的因素逐项进行了优化,并对电极进行了相关表征。实验研究表明,在最佳优化条件下,所构建的传感器,具有良好的稳定性和再现性,同时还能显著提高识别模板分子SCZ的能力,并放大了对SCZ的电流响应。经差分脉冲伏安法测得SCZ的还原峰电流随SCZ浓度线性增加,在1. 0×1. 0~(-6)～3. 0×10~(-4)mol/L和1. 91×10~(-8)～1. 0×1. 0~(-6)mol/L范围均呈良好线性关系,检出限(DL)为1. 72×10~(-8)mol/L。此外,该传感器还检测了药物片剂及生物样品中SCZ,具有良好的精密度和回收率。
In order to rapidly detect the content of drug molecule Secnidazole( SCZ),a carbon paste electrode electrochemical sensor based on multi-walled carbon nanotubes( MWCNTs) and boron embedded double-layer molecularly imprinted composite membrane( B-MICMs) was constructed. The factors affecting the performance of the sensor were optimized item by item,and the electrodes were characterized. The experimental results show that under the optimal optimization conditions,the constructed sensor has good stability and reproducibility,and can significantly improve the ability to identify the template molecule SCZ and amplify the current response to the SCZ. The reduction peak current of SCZ measured linearly with SCZ concentration by differential pulse voltammetry,in the range of 1. 0×1. 0~(-6)～ 3. 0×10~(-4) mol/L and 1. 91×10~(-8)～ 1. 0×1. 0~(-6) mol/L. All showed a good linear relationship with a detection limit( DL) of 1. 72×10~(-8) mol/L. In addition,the sensor also detects SCZ in drug tablets and biological samples with good precision and recovery.
In order to rapidly detect the content of drug molecule Secnidazole( SCZ),a carbon paste electrode electrochemical sensor based on multi-walled carbon nanotubes( MWCNTs) and boron embedded double-layer molecularly imprinted composite membrane( B-MICMs) was constructed. The factors affecting the performance of the sensor were optimized item by item,and the electrodes were characterized. The experimental results show that under the optimal optimization conditions,the constructed sensor has good stability and reproducibility,and can significantly improve the ability to identify the template molecule SCZ and amplify the current response to the SCZ. The reduction peak current of SCZ measured linearly with SCZ concentration by differential pulse voltammetry,in the range of 1. 0×1. 0~(-6)～ 3. 0×10~(-4) mol/L and 1. 91×10~(-8)～ 1. 0×1. 0~(-6) mol/L. All showed a good linear relationship with a detection limit( DL) of 1. 72×10~(-8) mol/L. In addition,the sensor also detects SCZ in drug tablets and biological samples with good precision and recovery.
引文
[1]NYIRJESY P,SCHWEBKE J R.Secnidazole:next-generation antimicrobial agent for bacterial vaginosis treatment[J].Future microbiology,2018,13(5):507.
[2] HILLIER S L,NYIRJESY P,WALDBAUM A S,et al.Secnidazole treatment of bacterial vaginosis:a randomized controlled trial[J]. Obstetrics and gynecology,2017,130(2):379-386.
[3]VIDEAU D,NIEL G,SIBOULET A,et al.Secnidazole,a5-nitroimidazole derivative with a long half-life[J].British journal of venereal diseases,1978,54(2):77-80.
[4]EDWARDS D I.Nitroimidazole drugs-action and resistance mechanisms I. mechanisms of action[J]. The journal of antimicrobial chemotherapy,1993,31(1):9-20.
[5]MARCIBIO M R,RAISER A L,FUMAGALLI L P,et al.Determinnation and validation of secnidazole in tablets by UV spectrophotometric[J]. Bioscience journal,2017,33(5):1351-1361.
[6] SUN H W,WANG F C,AI L F. Simultaneous determination of seven nitroimidazole residues in meat by using HPLC-UV detection with solid-phase extraction[J].Journal of chromatography B:analytical Technologies in the biomedical and life sciences,2007,857(2):296-300.
[7] BHATIA S C,SHANBHAG V D. Electron-capture gas chromatographic assays of 5-nitroimidazole class of antimicrobials in blood[J].Journal of chromatography biomedical sciences and applications,1984,305(2):325-334.
[8]SAHOO D K,SAHU P K.Chemometric approach for RPHPLC determination of azithromycin,secnidazole,and fluconazole using response surface methodology[J].Journal of liquid chromatography and related technologies,2015,38(6):750-758.
[9]RUBIES A,SANS G,KUMAR P.High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry[J]. Analytical and bioanalytical chemistry,2015,407(15):4411-4421.
[10]LIAO S,WENG Q.Rapid separation and determination of metronidazole benzoate and other antiprotozoal drugs by pressurized capillary electrochromatography[J].Journal of chemistry,2018,2018:1-7.
[11]EL-SAYED G O,YASIN S A,Badawy A A E.Determination of secnidazole in tablets and human serum by cathodic adsorptive stripping voltammetry[J].Arabian journal of chemistry,2010,3:167-172.
[12]LU B,XIA J F,WANG Z H,et al.Molecularly imprinted electrochemical sensor based on an electrode modified with imprinted pyrrole film immobilized onβ-cyclodextrin/gold nanoparticles/graphene layer[J].Rsc advances,2015,5:82930-82935.
[13] LEITE F R F,SANTOS W D J R,KUBOTA L T.Selective determination of caffeic acid in wines with electrochemical sensor based on molecularly imprinted siloxanes[J].Sensors and actuators B:chemical,2014,193:238-246.
[14]文艺,袁亚莉,李乐,等.介孔硅材料组装DNA电化学生物膜电极的制备[J].南华大学学报(自然科学版),2017,31(1):85-90.
[15]ATAR N,YOLA M L,EREN T.Sensitive determination of citrinin based on molecular imprinted electrochemical sensor[J].Applied surface science,2016,362:315-322.
[16]XIAO N,DENG J,CHENG J,et al.Carbon paste electrode modified with duplex molecularly imprinted polymer hybrid film for metronidazole detection[J].Biosensors and bioelectronics,2016,81:54-60.
[17]DENG J,JU S,LIU Y,et al.Highly sensitive and selective determination of melamine in milk using glassy carbon electrode modified with molecularly imprinted copolymer[J]. Food analytical methods,2015,8(10):2437-2446.
[2] HILLIER S L,NYIRJESY P,WALDBAUM A S,et al.Secnidazole treatment of bacterial vaginosis:a randomized controlled trial[J]. Obstetrics and gynecology,2017,130(2):379-386.
[3]VIDEAU D,NIEL G,SIBOULET A,et al.Secnidazole,a5-nitroimidazole derivative with a long half-life[J].British journal of venereal diseases,1978,54(2):77-80.
[4]EDWARDS D I.Nitroimidazole drugs-action and resistance mechanisms I. mechanisms of action[J]. The journal of antimicrobial chemotherapy,1993,31(1):9-20.
[5]MARCIBIO M R,RAISER A L,FUMAGALLI L P,et al.Determinnation and validation of secnidazole in tablets by UV spectrophotometric[J]. Bioscience journal,2017,33(5):1351-1361.
[6] SUN H W,WANG F C,AI L F. Simultaneous determination of seven nitroimidazole residues in meat by using HPLC-UV detection with solid-phase extraction[J].Journal of chromatography B:analytical Technologies in the biomedical and life sciences,2007,857(2):296-300.
[7] BHATIA S C,SHANBHAG V D. Electron-capture gas chromatographic assays of 5-nitroimidazole class of antimicrobials in blood[J].Journal of chromatography biomedical sciences and applications,1984,305(2):325-334.
[8]SAHOO D K,SAHU P K.Chemometric approach for RPHPLC determination of azithromycin,secnidazole,and fluconazole using response surface methodology[J].Journal of liquid chromatography and related technologies,2015,38(6):750-758.
[9]RUBIES A,SANS G,KUMAR P.High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry[J]. Analytical and bioanalytical chemistry,2015,407(15):4411-4421.
[10]LIAO S,WENG Q.Rapid separation and determination of metronidazole benzoate and other antiprotozoal drugs by pressurized capillary electrochromatography[J].Journal of chemistry,2018,2018:1-7.
[11]EL-SAYED G O,YASIN S A,Badawy A A E.Determination of secnidazole in tablets and human serum by cathodic adsorptive stripping voltammetry[J].Arabian journal of chemistry,2010,3:167-172.
[12]LU B,XIA J F,WANG Z H,et al.Molecularly imprinted electrochemical sensor based on an electrode modified with imprinted pyrrole film immobilized onβ-cyclodextrin/gold nanoparticles/graphene layer[J].Rsc advances,2015,5:82930-82935.
[13] LEITE F R F,SANTOS W D J R,KUBOTA L T.Selective determination of caffeic acid in wines with electrochemical sensor based on molecularly imprinted siloxanes[J].Sensors and actuators B:chemical,2014,193:238-246.
[14]文艺,袁亚莉,李乐,等.介孔硅材料组装DNA电化学生物膜电极的制备[J].南华大学学报(自然科学版),2017,31(1):85-90.
[15]ATAR N,YOLA M L,EREN T.Sensitive determination of citrinin based on molecular imprinted electrochemical sensor[J].Applied surface science,2016,362:315-322.
[16]XIAO N,DENG J,CHENG J,et al.Carbon paste electrode modified with duplex molecularly imprinted polymer hybrid film for metronidazole detection[J].Biosensors and bioelectronics,2016,81:54-60.
[17]DENG J,JU S,LIU Y,et al.Highly sensitive and selective determination of melamine in milk using glassy carbon electrode modified with molecularly imprinted copolymer[J]. Food analytical methods,2015,8(10):2437-2446.