抑制Fe~(2+)-H_2O_2-孔雀石绿褪色光度法测定挂面中的丙二醛
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摘要
Fe~(2+)-H_2O_2在酸性介质中氧化孔雀石绿反应褪色,但丙二醛加入Fe~(2+)-H_2O_2-孔雀石绿组成体系抑制褪色反应,且体系的吸光值随着丙二醛的含量增加呈线性增长。据此原理建立可见光分光光度法测定溶液中丙二醛的含量。在最优实验条件下,波长λ=620 nm,175μL硫酸、40μL硫酸亚铁、400μL孔雀石绿,140μL过氧化氢,反应时间为15 min、反应温度35℃时,线性回归方程为:ΔA620 nm=0.197 1ρ-0.014 3(ρ:μg/mL),线性范围为0.125~3.50μg/mL,检出限(3S/K)为0.068μg/mL,该方法分析挂面中的丙二醛的含量,回收率在95.7%~107.1%之间,分析方法可行。
The inhibition effect of malondialdehyde on the fading of malachite green oxalate solution by Fe~(2+)-H_2O_2 oxidation was investigated in sulfuric acid medium.The absorbance value of solution increases with the concentration of malondialdehyde in solution.A method for the determination of malondialdehyde in the liquid by spectrophotometry was established. The optimum experimental conditions were as follows: the maximum fading wavelength was located at 620 nm; the volume of sulfuric was 175 μL; the volume of ferrous sulfate was 40 μL; the volume of Malachite Green was 400 μL; the volume of perhydrol was 140 μL; the reaction time was 15 min; the reaction temperature was 35 ℃. The linear regression equation was ΔA620 nm= 0. 197 1ρ-0. 014 3(ρ: μg/mL),the linear range of 0. 125 to 3. 50 μg/mL,and the detection of limit was 0. 068 μg/mL.The recovery of MDA was 95. 7% ~107. 1% in the analysis noodles via this method,and this method is feasible.
The inhibition effect of malondialdehyde on the fading of malachite green oxalate solution by Fe~(2+)-H_2O_2 oxidation was investigated in sulfuric acid medium.The absorbance value of solution increases with the concentration of malondialdehyde in solution.A method for the determination of malondialdehyde in the liquid by spectrophotometry was established. The optimum experimental conditions were as follows: the maximum fading wavelength was located at 620 nm; the volume of sulfuric was 175 μL; the volume of ferrous sulfate was 40 μL; the volume of Malachite Green was 400 μL; the volume of perhydrol was 140 μL; the reaction time was 15 min; the reaction temperature was 35 ℃. The linear regression equation was ΔA620 nm= 0. 197 1ρ-0. 014 3(ρ: μg/mL),the linear range of 0. 125 to 3. 50 μg/mL,and the detection of limit was 0. 068 μg/mL.The recovery of MDA was 95. 7% ~107. 1% in the analysis noodles via this method,and this method is feasible.
引文
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[8]蒋小华,谢运昌,李娟,等.气相色谱-质谱法测定大鼠血浆和肝匀浆中丙二醛含量[J].理化检验-化学分册,2013,49(5):573-576.
[9]凌绍明,欧阳辉祥,黄宝敬.基于固绿褪色反应的分光光度法测定双酚A的含量[J].理化检验-化学分册,2016,52(6):625-628.
[10]雷禄,凌绍明,刘惠斌.固绿分光光度法测定土壤中的痕量四溴双酚A的含量[J].化学试剂,2017,39(2):179-182.
[2]任雪梅,王文特,田洪芸,等.比色法测定鸭油中的丙二醛[J].山东农业科学,2014,46(1):117-119.
[3]高俊,陈季旺,周琦,等.挂面中丙二醛含量测定方法的研究[J].中国粮油学报,2012,27(3):115-120.
[4]杨秀军,马璐,凌绍明.固绿褪色反应体系分光光度法测定食品中丙二醛[J].粮油食品科技,2017,25(3):61-64.
[5]刘彬,齐云,李蒙,等.分光光度法与荧光法测定肝组织丙二醛含量的比较研究[J].中国药理学通报,2010,26(12):1 674-1 677.
[6]余巍,赵永锋,吴玉杰,等.高效液相色谱法测定食品中丙二醛[J].分析试验室,2014,33(4):445-447.
[7]杨海锋,刘丹,杨俊花,等.高效液相色谱法测定饲料中的丙二醛含量[J].上海农业学报,2013,29(4):14-17.
[8]蒋小华,谢运昌,李娟,等.气相色谱-质谱法测定大鼠血浆和肝匀浆中丙二醛含量[J].理化检验-化学分册,2013,49(5):573-576.
[9]凌绍明,欧阳辉祥,黄宝敬.基于固绿褪色反应的分光光度法测定双酚A的含量[J].理化检验-化学分册,2016,52(6):625-628.
[10]雷禄,凌绍明,刘惠斌.固绿分光光度法测定土壤中的痕量四溴双酚A的含量[J].化学试剂,2017,39(2):179-182.