梧桐籽抗氧化活性成分提取工艺的优化
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摘要
以梧桐籽为研究对象,经过挑选、清洗、去壳、干燥、粉碎等工序制成初级样品,以梧桐子提取液的二苯基苦酰肼基自由基(diphenyl picryl hydrazinyl radical,DPPH)清除率为指标,对提取溶剂、提取时间、料液比、提取温度等因素进行了单因素试验,并利用响应面法对试验设计进行了优化。单因素试验表明,梧桐籽抗氧化活性成分的最佳提取试剂为乙酸乙酯、最佳提取时间为50min、最优料液比为1∶6、最佳提取温度为60℃;响应面优化试验表明,梧桐籽抗氧化活性成分提取液最佳提取工艺为:提取溶剂乙酸乙酯、提取时间45min、料液比1∶5、提取温度55℃,与单因素试验结果基本吻合;最佳提取工艺条件下,梧桐籽乙酸乙酯提取液(200mg/mL)对DPPH的清除率为94.25%,最佳提取工艺下梧桐籽提取物的DPPH清除率相当于147.39mg/L的维生素E(VE)的DPPH清除效果,这说明梧桐籽乙酸乙酯提取物具有很强的抗氧化能力。
The phoenix tree seeds were taken as the research object.In the first place,the primary samples of phoenix tree seeds were prepared following the processing of selection,cleaning,shelling,drying and grinding,etc.In the next place,single factor experiments were conducted to test the premium extraction factors containing extraction solvent,extraction time,liquor ratio and extracting temperature using the scavenging activity of(diphenyl picryl hydrazinyl radical,DPPH)as indexes.In the end,the experiment design was optimized through Response Surface Methodology.The results of single factor experiment determining the antioxidative ingredients contained within phoenix tree seed showed that the optimum extraction solvent was ethyl acetate,the optimum extraction time was 50 min.,the optimum solid to liquor ratio was 1∶6,the best extracting temperature was 60℃.The results,which were consistent with the results of single factor experiments,tested in the optimum extraction process of antioxidative ingredients contained within phoenix tree seeds indicated that the extraction solvent was ethyl acetate,extraction time was 45 min.,solid to liquor ratio was 1∶5 and extraction temperature was 55℃.Under the optimum extraction process,the scavenging rate belonging to phoenix tree seeds extract liquid(200 mg/mL)of free radical DPPH was 94.25%.The DPPH scavenging effect of phoenix tree seeds extraction under the optimum extraction process was equivalent to that of 147.39 mg/L vitamin E(VE),which indicated that the extraction of phoenix seeds possessed a strong antioxidant activity.
The phoenix tree seeds were taken as the research object.In the first place,the primary samples of phoenix tree seeds were prepared following the processing of selection,cleaning,shelling,drying and grinding,etc.In the next place,single factor experiments were conducted to test the premium extraction factors containing extraction solvent,extraction time,liquor ratio and extracting temperature using the scavenging activity of(diphenyl picryl hydrazinyl radical,DPPH)as indexes.In the end,the experiment design was optimized through Response Surface Methodology.The results of single factor experiment determining the antioxidative ingredients contained within phoenix tree seed showed that the optimum extraction solvent was ethyl acetate,the optimum extraction time was 50 min.,the optimum solid to liquor ratio was 1∶6,the best extracting temperature was 60℃.The results,which were consistent with the results of single factor experiments,tested in the optimum extraction process of antioxidative ingredients contained within phoenix tree seeds indicated that the extraction solvent was ethyl acetate,extraction time was 45 min.,solid to liquor ratio was 1∶5 and extraction temperature was 55℃.Under the optimum extraction process,the scavenging rate belonging to phoenix tree seeds extract liquid(200 mg/mL)of free radical DPPH was 94.25%.The DPPH scavenging effect of phoenix tree seeds extraction under the optimum extraction process was equivalent to that of 147.39 mg/L vitamin E(VE),which indicated that the extraction of phoenix seeds possessed a strong antioxidant activity.
引文
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[34]华艳宏,庞春花,张永清,等.藜麦种子不同溶剂提取物及其抗氧化活性[J].江苏农业科学,2018,46(20):225-228
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[36]吴双,印敏,单宇,等.海滨锦葵提取物体外抗氧化作用研究[J].食品科技,2014,39(05):193-197
[2]李时珍.本草纲目[M].上海:上海辞书出版社,2006:536-537
[3]刘东.治疗鼻出血新药梧桐籽冲剂[J].西北药学杂志,1986,1(1):64-64
[4]杨亚惠,李宝棣,王玉琦,等.梧桐籽治疗鼻衄有效成分的分析[J].陕西新医药,1983,12(10):59-60
[5]姚鸿萍,陈丽梅,曹永孝,等.梧桐籽颗粒剂的促凝血作用[J].中成药,2006,28(2):267-268
[6]李振富,杨亚惠,李宝棣,等.梧桐籽冲剂治疗鼻出血(320例临床疗效观察及动物试验研究)[J].陕西新医药,1986,15(11):6-8
[7]车锡平,王秉文.梧桐籽的止血作用[J].西安交通大学学报(医学版),1983,4(2):142-143
[8]邱培伦,王美纳,邓秀玲,等.梧桐籽的降压作用[J].西安交通大学学报(医学版),1984,5(3):263-267
[9]栗亚琼.梧桐籽油提取工艺研究及其脂肪酸的气相色谱分析[D].郑州:河南工业大学,2015
[10]原志德,贾洪东.梧桐籽治愈前列腺肥大所致尿潴留一例[J].赤脚医生杂志,1978,154(4):4-5
[11]刘海春.一种具有促消化、保胃、提高智力及免疫力的梧桐籽食用油[P].中国专利:CN106906042A,2017-06-30
[12]王锦前.抗衰老药物及其制备方法[P].中国专利:CN1483438,2004-03-24
[13]王振宇,赵海田.生物活性成分分离技术[M].哈尔滨:哈尔滨工业大学出版社,2015:119-349
[14]董明芝,袁鹰,欧阳卉.梧桐籽总黄酮提取工艺研究[J].陕西中医,2015,36(6):737-738
[15]CHEN Y H,WANG Z,XU S Y.Antioxidant Properties of Wheat Germ Protein Hydrolysates Evaluated in vitro[J].Journal of Central South University of Technology(English E-dition),2006,13(2):160-165
[16]MARLA Luther,JOHN Parry,JEFFREY Moore,et al.Inhibitory Effect of Chardonnay and Black Raspberry Seed Extracts on Lipid Oxidation in Fish Oil and their Radical Scavenging and Antimicrobial Properties[J].Food Chemistry,2007,104(3):1065-1073
[17]CHOI J S,LEE H J,KANGS.Alaternin,Cassiaside and Rubrofusarin Gentiobioside,Radical Scavenging Principles from the Seeds of Cassia Tora on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical.[J].Archives of Pharmacal Research,1994,17(6):462-466
[18]ALJuhaimi,ADIAMO,GHAFOOR,et al.Optimization of Ultrasonic-assisted Extraction of Phenolic Compounds from Fenugreek(Trigonella foenum-graecum L.)seed[J].CYTA-Journal of Food,2016,14(3):369-374
[19]ALI BAS Wani,DEVINDER Kaur,IDREES Ahmed,et al.Extraction Optimization of Watermelon Seed Protein Using Response Surface Methodology[J].LWT-Food Science and Technology,2008,41(8):1514-1520
[20]M.M.K.Bhuiya,M.G.Rasul,M.M.K.Khan,et al.Optimization of Oil Extraction Process from Australian Native Beauty Leaf Seed(Calophyllum Inophyllum)[J].Energy Procedia,2015,75(1):56-61
[21]ZHANG B,CHEN Y Z,WEI X F,et al.Optimization of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp(Ctenopharyngodon idella)by Response Surface Methodology[J].International Journal of Food Engineering,2011,6(3):1-18
[22]MIROSLAY Ondrejoviˇc,DANIELA Chmelová,TIBOR Maliar.Optimization of the Antioxidant Extraction from Eleutherococcus Senticosus Roots by Response Surface Methodology[J].Nova Biotechnologica et Chimica,2013,12(1):30-38
[23]NDER Aybastler,ESRA I爧lk,SALIHA爦ahin,et al.Optimization of Ultrasonic-assisted Extraction of Antioxidant Compounds from Blackberry Leaves Using Response Surface Methodology[J].Industrial Crops&Amp Products,2013,44(1):558-565
[24]杨芙莲,翟小艳.荞麦种子发芽期间总黄酮含量的测定[J].粮食与油脂,2014,27(12):41-43
[25]ZGAI X,JIN Q,XIANG H Y,et al.Orthogonal Test Optimization of Extraction technology of Total Flavonoids from Fruits and Leaves of Prunus Mume Sieb.and Its in Vitro Anti-oxidation Activity[J].Medicinal Plant,2017,8(02):25-31+36
[26]ANTHIA Matsakidou,FANI Th.Mantzouridou,VASSILIOSKiosseoglou.Optimization of Water Extraction of Naturally E-mulsified Oil from Maize Germ[J].LWT-Food Science and Technology,2015,63(1):206-213
[27]郭宏垚,李冬,雷雄,等.花椒多酚提取工艺响应面优化及动力学分析[J].食品科学,2018,39(2):247-253
[28]王升匀,杨政敏.桂花种子多糖和多酚含量测定及体外清除自由基和亚硝基活性研究[J].亚太传统医药,2018,14(08):65-68
[29]张永芳,张琪,王润梅,张弘弛.茚三酮呈色法测定谷子种子中的游离氨基酸含量[J].种子,2014,33(01):111-113+126
[30]唐开永,周鸿翔,田娅玲,邹聪丽.低浓度小分子多肽含量测定方法的比较研究[J].食品研究与开发,2018,39(16):144-148
[32]邱德文,吴家荣,夏同珩.本草纲目彩色药图[M].贵州:贵州科技出版社,1998:729
[33]李现日,张杰,张英美,等.金花葵花黄酮提取物不同溶剂萃取物的抗氧化活性研究[J/OL].食品工业科技:1-11[2019-05-22]http://kns.cnki.net/kcms/detail/11.1759.TS.20190124.1558.002.html
[34]华艳宏,庞春花,张永清,等.藜麦种子不同溶剂提取物及其抗氧化活性[J].江苏农业科学,2018,46(20):225-228
[35]李治明,高银环,梁振益.皇后葵果实提取物的抗氧化活性研究[J].广州化工,2015,43(24):79-80+89
[36]吴双,印敏,单宇,等.海滨锦葵提取物体外抗氧化作用研究[J].食品科技,2014,39(05):193-197