基于PCA及CA的唐古特大黄蒽醌类、二蒽酮类及鞣质类含量研究
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摘要
大黄药材中蒽醌类、二蒽酮类及鞣质类是其主要有效成分。该文以唐古特大黄主产地药材为研究对象,采用HPLC测定游离和结合蒽醌类、二蒽酮类及鞣质类成分的含量,用多重比较、主成分分析PCA及对应分析CA探究3类成分积累规律。结果显示,不同产地、不同生长方式(野生和家种)的大黄化学成分含量差异达到显著性水平(P<0. 05)。产于白玉县、小金县及若尔盖县的分别在儿茶素、大黄酸及番泻苷A的积累上具有明显的优势;唐古特大黄野生品与栽培品主成分构成不同,野生条件下,有利于结合型蒽醌组分的积累;规范化种植大黄的3类活性成分积累量优于散户零星种植。因此,唐古特大黄栽培基地选择应充分考虑产地对大黄化学成分积累的影响。规范化种植方式较优,但即使是在此条件下,唐古特大黄栽培品也不及野生品。
Anthraquinones,dianthrones and tannins are the main active ingredients of Rheum tanguticum. In this study the three components were determined by HPLC,and the results were analyzed by multiple comparisons,principal components analysis(PCA)and correspondence analysis(CA). The results showed that the contents of components in different growing areas and types(wild and cultivated) reached a significant level(P<0. 05). Baiyu county,Xiaojin county and Ruoergai county had obvious advantages in the accumulation of catechin hydrate,rhien and sensenoside A respectively. The principal component was different in two growing type and the wild environment was conducive to combined anthraquinones accumulation. For active components,normalized planting was better than retail cultivating. Therefore,the effect on the accumulation of chemical components in Rh. tangusticum,should be taken into full account in the selection of the cultural base of Rh. tanguticum. The standardized cultivating is superior to retail cultivating in terms of the accumulation of active ingredients,and standardized planting is inferior to the wild.
Anthraquinones,dianthrones and tannins are the main active ingredients of Rheum tanguticum. In this study the three components were determined by HPLC,and the results were analyzed by multiple comparisons,principal components analysis(PCA)and correspondence analysis(CA). The results showed that the contents of components in different growing areas and types(wild and cultivated) reached a significant level(P<0. 05). Baiyu county,Xiaojin county and Ruoergai county had obvious advantages in the accumulation of catechin hydrate,rhien and sensenoside A respectively. The principal component was different in two growing type and the wild environment was conducive to combined anthraquinones accumulation. For active components,normalized planting was better than retail cultivating. Therefore,the effect on the accumulation of chemical components in Rh. tangusticum,should be taken into full account in the selection of the cultural base of Rh. tanguticum. The standardized cultivating is superior to retail cultivating in terms of the accumulation of active ingredients,and standardized planting is inferior to the wild.
引文
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[2]王亦君,冯舒涵,程锦堂,等.大黄蒽醌类化学成分和药理作用研究进展[J].中国实验方剂学杂志,2018,24(13):227.
[3]李锋,王胜春,王新,等.大黄泻下效应的药理学新解释[J].中国中药杂志,2008,24(4):481.
[4] Meng Jie,Ni Junjun,Wu Zhou,et al. The critical role of IL-10in the antineuroinflammatory and antioxidative effects of Rheum tanguticum on activated microglia[J]. Acta Neuropharm,2018(2):1.
[5]容悦莹,冯素香,吴纯伟,等.大黄化学成分的药动学及其代谢的研究进展[J].中国实验方剂学杂志,2016,22(10):199.
[6]王伽伯,张学儒,肖小河,等.基于化学分析的大黄药材商品规格划分的科学合理性研究[J].中国中药杂志,2010,35(4):470.
[7]芦雅丽,赵建邦,宋平顺.不同产地大黄药材中没食子酸和儿茶素的比较[J].中成药,2011,33(5):832.
[8]孙佩,李敏,杨小多,等. HPLC法测定大黄药材和饮片中番泻苷A和番泻苷B的含量[J].成都中医药大学学报,2008,31(3):51.
[9]刘照德,林海明.对应分析法的改进与应用[J].数理统计与管理,2018,37(2):243.
[10]葛建华,刘训红,许虎,等.基于化学分析的不同品种大黄区分研究[J].中国中药杂志,2015,40(12):2309.
[11]虞文妹,武煦峰,徐方铭.基于药效学的青海唐古特大黄的野生和栽培品比较研究[J].时珍国医国药,2012,23(12):3210.