红芪复方对CCl_4致小鼠肝损伤防治作用的筛方研究
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摘要
目的筛选防治肝损伤效果最佳的红芪复方。方法皮下注射四氯化碳(CCl_4)致小鼠肝损伤,分别给予小鼠4个红芪复方(原药材20 g·kg~(-1),每日1次)灌胃,7周后测定小鼠血清中的谷丙转氨酶(ALT)和谷草转氨酶(AST);测定肝组织匀浆中的超氧化物歧化酶(SOD)和丙二醛(MDA);采用苏木素-伊红(HE)染色观察肝组织病理变化。结果 CCl_4所致小鼠肝损伤效果明显;4个红芪复方对CCl_4致小鼠肝损伤药效显著,但药效活性存在差异,其中红芪复方三的效果最佳。结论红芪复方三具有防治小鼠肝损伤的作用,具有进一步研究的价值。
Objective To screen the prescription of compound Chinese medicine of Radix Hedysari used for the prevention and therapeutics on carbon tetrachloride induced liver injury in mice.Methods Liver injury was induced by subcutaneous injection of carbon tetrachloride(CCl_4)-peanut oil in mice.At the same time,4 prescriptions of compound traditional Chinese medicine of Radix Hedysari(20 g·kg~(-1),once each day) were given intragastrically.After 7 weeks,serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST),liver superoxide dismutase(SOD) and malondialdehyde(MDA) were detected.Histological examination was performed using hematoxylin-eosin staining and light microscopy.Results The effect of CCl_4 on liver injury in mice was obvious,the effect of the 4 prescriptions of compound traditional Chinese medicine of Radix Hedysari on liver injury induced by CCl_4 was significant,but there were differences in their pharmacodynamic activities,among which the prescription 3 was the best.Conclusion The prescription 3 of compound traditional Chinese medicine of Radix Hedysari has the function of preventing and treating liver injury in mice and has the value of further study.
Objective To screen the prescription of compound Chinese medicine of Radix Hedysari used for the prevention and therapeutics on carbon tetrachloride induced liver injury in mice.Methods Liver injury was induced by subcutaneous injection of carbon tetrachloride(CCl_4)-peanut oil in mice.At the same time,4 prescriptions of compound traditional Chinese medicine of Radix Hedysari(20 g·kg~(-1),once each day) were given intragastrically.After 7 weeks,serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST),liver superoxide dismutase(SOD) and malondialdehyde(MDA) were detected.Histological examination was performed using hematoxylin-eosin staining and light microscopy.Results The effect of CCl_4 on liver injury in mice was obvious,the effect of the 4 prescriptions of compound traditional Chinese medicine of Radix Hedysari on liver injury induced by CCl_4 was significant,but there were differences in their pharmacodynamic activities,among which the prescription 3 was the best.Conclusion The prescription 3 of compound traditional Chinese medicine of Radix Hedysari has the function of preventing and treating liver injury in mice and has the value of further study.
引文
[1] 何建成,王自立,耿铁海.舒肝消积丸治疗慢性乙型肝炎的临床研究[J].中成药,1996,18(5):24-26,53-54.
[2] 孙启祥,吴爱光,郑云霞.舒肝消积丸对小鼠免疫功能的影响[J].甘肃中医学院学报,1989,(4):49-51.
[3] 孙慧莉,张宪云.舒肝消积丸对肝损伤保护作用的药理研究[J].中成药,1990,12(8):26-27.
[4] 黄晓菡,曾文铤,朱科伦,等.丹芪活肝胶囊对D-氨基半乳糖致大鼠肝损伤的保护作用[J].广州医药,2009,40(5):44-47.
[5] 黄小强,张雪,熊丽樱,等.泽泻多糖对四氯化碳致小鼠急性肝损伤的保护作用[J].时珍国医国药,2017,28(6):1300-1302.
[6] 李伦,钟伟超,梁伟海,等.栀子大黄汤对四氯化碳致小鼠急性肝损伤的保护作用[J].中国实验方剂学杂志,2016,22(12):108-112.
[7] 吴雅茗,唐灵芝,翁乐斌,等.红茶菌对四氯化碳致急性肝损伤小鼠的保护作用[J].西北药学杂志,2017,32(2):175-180.
[8] 王瑶,钟希文,张文霞.鳄嘴花对CCl4诱导的小鼠急性肝损伤的保护作用[J].时珍国医国药,2017,28(1):98-99.
[9] 冯芹,夏文凯,王现珍,等.连翘苷元对四氯化碳大鼠急性肝损伤的保护作用[J].中国药理学通报,2015,31(3):426-430.
[10] 杨宏昕,白音夫,常亮,等.红花清肝十三味对CCl4诱导大鼠肝损伤纤维化的作用[J].西北药学杂志,2018,33(3):349-352.
[11] 黄思茂,曹后康,高雅,等.金花茶多糖对四氯化碳致小鼠急性肝损伤的保护作用及其机制的研究[J].中药药理与临床,2016,32(6):117-120.
[12] 王跃峰,张可锋,周雨晴,等.拳卷地钱总黄酮对四氯化碳致急性肝损伤大鼠的保护作用及其作用机制[J].时珍国医国药,2017,28(2):277-279.
[13] 刘丽,李军,訾慧,等.保肝颗粒对小鼠急性化学性肝损伤的保护作用[J].中国实验方剂学杂志,2015,21(20):123-126.
[14] 杨婷婷,王伟,李国全,等.水飞蓟宾对小鼠急性肝损伤的保护作用[J].中国实验方剂学杂志,2016,22(10):102-106.
[15] 魏春华,邵坚,罗光明.栀子生品及炮制品对CCl4致大鼠急性肝损伤保护作用的比较[J].中国实验方剂学杂志,2016,22(17):7-10.
[16] 钟萍,李萍,张昕蕾.当归多糖对小鼠化学性肝损伤抗氧化酶及一氧化氮含量的影响[J].现代预防医学,2011,38(22):4725-4727.
[17] 李丽,莫遗盛,王冬梅,等.复方护肝口服液对小鼠急性化学性肝损伤的影响[J].时珍国医国药,2008,19(10):2363-2364.
[18] 国家药典委员会.中国药典:2015年:一部[S].北京:中国医药科技出版社,2015:152.
[2] 孙启祥,吴爱光,郑云霞.舒肝消积丸对小鼠免疫功能的影响[J].甘肃中医学院学报,1989,(4):49-51.
[3] 孙慧莉,张宪云.舒肝消积丸对肝损伤保护作用的药理研究[J].中成药,1990,12(8):26-27.
[4] 黄晓菡,曾文铤,朱科伦,等.丹芪活肝胶囊对D-氨基半乳糖致大鼠肝损伤的保护作用[J].广州医药,2009,40(5):44-47.
[5] 黄小强,张雪,熊丽樱,等.泽泻多糖对四氯化碳致小鼠急性肝损伤的保护作用[J].时珍国医国药,2017,28(6):1300-1302.
[6] 李伦,钟伟超,梁伟海,等.栀子大黄汤对四氯化碳致小鼠急性肝损伤的保护作用[J].中国实验方剂学杂志,2016,22(12):108-112.
[7] 吴雅茗,唐灵芝,翁乐斌,等.红茶菌对四氯化碳致急性肝损伤小鼠的保护作用[J].西北药学杂志,2017,32(2):175-180.
[8] 王瑶,钟希文,张文霞.鳄嘴花对CCl4诱导的小鼠急性肝损伤的保护作用[J].时珍国医国药,2017,28(1):98-99.
[9] 冯芹,夏文凯,王现珍,等.连翘苷元对四氯化碳大鼠急性肝损伤的保护作用[J].中国药理学通报,2015,31(3):426-430.
[10] 杨宏昕,白音夫,常亮,等.红花清肝十三味对CCl4诱导大鼠肝损伤纤维化的作用[J].西北药学杂志,2018,33(3):349-352.
[11] 黄思茂,曹后康,高雅,等.金花茶多糖对四氯化碳致小鼠急性肝损伤的保护作用及其机制的研究[J].中药药理与临床,2016,32(6):117-120.
[12] 王跃峰,张可锋,周雨晴,等.拳卷地钱总黄酮对四氯化碳致急性肝损伤大鼠的保护作用及其作用机制[J].时珍国医国药,2017,28(2):277-279.
[13] 刘丽,李军,訾慧,等.保肝颗粒对小鼠急性化学性肝损伤的保护作用[J].中国实验方剂学杂志,2015,21(20):123-126.
[14] 杨婷婷,王伟,李国全,等.水飞蓟宾对小鼠急性肝损伤的保护作用[J].中国实验方剂学杂志,2016,22(10):102-106.
[15] 魏春华,邵坚,罗光明.栀子生品及炮制品对CCl4致大鼠急性肝损伤保护作用的比较[J].中国实验方剂学杂志,2016,22(17):7-10.
[16] 钟萍,李萍,张昕蕾.当归多糖对小鼠化学性肝损伤抗氧化酶及一氧化氮含量的影响[J].现代预防医学,2011,38(22):4725-4727.
[17] 李丽,莫遗盛,王冬梅,等.复方护肝口服液对小鼠急性化学性肝损伤的影响[J].时珍国医国药,2008,19(10):2363-2364.
[18] 国家药典委员会.中国药典:2015年:一部[S].北京:中国医药科技出版社,2015:152.