加减葛花解酲汤对小鼠急性乙醇中毒的干预作用
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摘要
目的观察加减葛花解酲汤对小鼠急性乙醇中毒的干预作用。方法将40只昆明种雄性小鼠随机分为正常组,模型组,加减葛花解酲汤低、中、高剂量组,每组8只。采用白酒灌胃复制小鼠急性乙醇中毒模型。给药后处死小鼠,取全血测定乙醇含量,取肝脏组织匀浆后分别检测超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量。结果随着加减葛花解酲汤浓度的升高,急性乙醇中毒小鼠的醉酒耐受时间逐渐延长,醒酒时间逐渐缩短。与模型组比较,加减葛花解酲汤低、中、高剂量组小鼠全血乙醇浓度明显降低(P<0.05),加减葛花解酲汤中、高剂量组小鼠肝组织中SOD水平显著升高(P<0.05),高剂量组MDA水平显著降低(P<0.05)。结论加减葛花解酲汤能明显降低血液乙醇浓度和肝组织中MDA水平,升高SOD水平,具备较好的解酒功效,其解酒的机制可能与保护肝细胞的作用有关。
Objective To investigate the therapeutic effect of Modified Gehua Jiecheng Decoction on mice with acute alcoholism.Methods A total of 40 male Kunming mice were randomly divided into normal group,model group,and low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups,with 8 mice in each group.A mouse model of acute alcoholism was established by the administration of liquor by gavage;after drug treatment,the mice were sacrificed,and whole blood was collected to measure the contentof alcohol;liver tissue homogenate was used to measure the activity of superoxide dismutase(SOD)and malondialdehyde(MDA).Results With the increase in the concentration of Modified Gehua Jiecheng Decoction,there was a gradual increase in drunkenness tolerance time and a gradual reduction in sobering time in mice with acute alcoholism.Compared with the model group,the low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups had a significant reduction in the concentration of alcohol in blood(P<0.05);the middle-and high-dose Modified Gehua Jiecheng Decoction groups had a significant increase in the level of SOD in liver tissue(P<0.05),and the high-dose Modified Gehua Jiecheng Decoction group had a significant reduction in the level of MDA in liver tissue(P<0.05).Conclusion Modified Gehua Jiecheng Decoction can significantly reduce the concentration of alcohol in blood and the content of MDA in liver tissue and increase the activity of SOD.Therefore,it has a good antialcoholic effect,possibly by protecting hepatocytes.
Objective To investigate the therapeutic effect of Modified Gehua Jiecheng Decoction on mice with acute alcoholism.Methods A total of 40 male Kunming mice were randomly divided into normal group,model group,and low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups,with 8 mice in each group.A mouse model of acute alcoholism was established by the administration of liquor by gavage;after drug treatment,the mice were sacrificed,and whole blood was collected to measure the contentof alcohol;liver tissue homogenate was used to measure the activity of superoxide dismutase(SOD)and malondialdehyde(MDA).Results With the increase in the concentration of Modified Gehua Jiecheng Decoction,there was a gradual increase in drunkenness tolerance time and a gradual reduction in sobering time in mice with acute alcoholism.Compared with the model group,the low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups had a significant reduction in the concentration of alcohol in blood(P<0.05);the middle-and high-dose Modified Gehua Jiecheng Decoction groups had a significant increase in the level of SOD in liver tissue(P<0.05),and the high-dose Modified Gehua Jiecheng Decoction group had a significant reduction in the level of MDA in liver tissue(P<0.05).Conclusion Modified Gehua Jiecheng Decoction can significantly reduce the concentration of alcohol in blood and the content of MDA in liver tissue and increase the activity of SOD.Therefore,it has a good antialcoholic effect,possibly by protecting hepatocytes.
引文
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[2] 储全根,张登山,汪克明,等.李氏解酒方解酒作用的实验研究[J].安徽中医学院学报,2007,26(5):27-29.
[3] 汤子春,邹坤,汪鋆植,等.开口箭与筒鞘蛇菰提取物醒酒作用的实验研究[J].时珍国医国药,2006,17(11):2163-2165.
[4] MASALKAR P D,ABHANG S A.Oxidative stress and antioxidant status in patients with alcoholic liver disease[J].Clin Chim Acta,2005,355(1/2) :61-65.
[5] MEIER P,SEITZ H K.Age,alcohol metabolism and liver disease[J].Curr Opin Clin Nutr Metab Care,2008,11(1):21-26.
[6] 王心如.毒理学基础[M].北京:人民卫生出版社,2004:65-67.
[7] LV W P,WANG C,LI L,et al.Progress in studies of products of antiinebriation[J].Strait Pharm J,2009,21(6):811.
[8] 高学清,汪何雅,钱和,等.葛根和葛花对急性酒精中毒小鼠的解酒作用[J].食品与生物技术学报,2012,36(6):621-627.
[9] HAN Y O,HAN M J,PARK S H,et al.Protective effects of kakkalide from flos puerariae on ethanol-induced lethality and hepatic injury are dependent on its biotransformation by human intestinal mircoflora[J].Journal of Phamacological Sciences,2003,93(3):331-336.
[10] 王艳林,韩钰,钱京萍,等.枳椇子抗脂质过氧化作用的实验研究[J].中草药,1994,25(6):306-307.
[11] YOSHIKAWA K,TUMURAS S,YAMADA K,et al.Antisweet natural products:Ⅶ:Hodulosides Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ from the leaves of Hovenia dulcis Thunb[J].Chemical and Pharmaceutical Bulletin,2008,40(9):2287-2291.
[12] 韩钰,王艳林,樊玉谷,等.枳椇子对实验性肝损伤的保护作用[J].现代应用药学,1997,14(2):6-8.
[13] 柳海艳,钟赣生,李怡文,等.醇提和水提葛花枳椇子及其配伍对酒精性肝损伤大鼠肝脏抗氧化功能的影响[J].中华中医药杂志,2012,27(4):1181-1184.
[14] 毛中伏,谈硕彦,闫昌誉,等.解酒中药复方“肝易复”对小鼠急性酒精性肝损伤的保护作用[J].中药新药与临床药理,2016,27(5):660-665.