摘要
目的观察加减葛花解酲汤对小鼠急性乙醇中毒的干预作用。方法将40只昆明种雄性小鼠随机分为正常组,模型组,加减葛花解酲汤低、中、高剂量组,每组8只。采用白酒灌胃复制小鼠急性乙醇中毒模型。给药后处死小鼠,取全血测定乙醇含量,取肝脏组织匀浆后分别检测超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量。结果随着加减葛花解酲汤浓度的升高,急性乙醇中毒小鼠的醉酒耐受时间逐渐延长,醒酒时间逐渐缩短。与模型组比较,加减葛花解酲汤低、中、高剂量组小鼠全血乙醇浓度明显降低(P<0.05),加减葛花解酲汤中、高剂量组小鼠肝组织中SOD水平显著升高(P<0.05),高剂量组MDA水平显著降低(P<0.05)。结论加减葛花解酲汤能明显降低血液乙醇浓度和肝组织中MDA水平,升高SOD水平,具备较好的解酒功效,其解酒的机制可能与保护肝细胞的作用有关。
Objective To investigate the therapeutic effect of Modified Gehua Jiecheng Decoction on mice with acute alcoholism.Methods A total of 40 male Kunming mice were randomly divided into normal group,model group,and low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups,with 8 mice in each group.A mouse model of acute alcoholism was established by the administration of liquor by gavage;after drug treatment,the mice were sacrificed,and whole blood was collected to measure the contentof alcohol;liver tissue homogenate was used to measure the activity of superoxide dismutase(SOD)and malondialdehyde(MDA).Results With the increase in the concentration of Modified Gehua Jiecheng Decoction,there was a gradual increase in drunkenness tolerance time and a gradual reduction in sobering time in mice with acute alcoholism.Compared with the model group,the low-,middle-,and high-dose Modified Gehua Jiecheng Decoction groups had a significant reduction in the concentration of alcohol in blood(P<0.05);the middle-and high-dose Modified Gehua Jiecheng Decoction groups had a significant increase in the level of SOD in liver tissue(P<0.05),and the high-dose Modified Gehua Jiecheng Decoction group had a significant reduction in the level of MDA in liver tissue(P<0.05).Conclusion Modified Gehua Jiecheng Decoction can significantly reduce the concentration of alcohol in blood and the content of MDA in liver tissue and increase the activity of SOD.Therefore,it has a good antialcoholic effect,possibly by protecting hepatocytes.
引文
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