雅安藏茶茶多糖对~(60)Co-γ射线辐照损伤小鼠抗氧化和造血功能的防护作用
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摘要
为探究雅安藏茶茶多糖对~(60)Co-γ射线辐射损伤小鼠抗辐射作用的影响,从雅安藏茶中提取茶多糖,用不同浓度(50、100、200 mg·kg-1·d-1)的茶多糖连续15 d灌胃小鼠,在第6天采用5Gy的~(60)Co-γ射线一次性全身辐照各组小鼠,灌胃第16天测定各组小鼠外周血细胞、肝脏组织总抗氧化能力(T-AOC)、总超强氧化物歧化酶(T-SOD)活性和总蛋白(TP)含量等指标。结果表明,与单纯辐照组相比,雅安藏茶茶多糖能显著提高辐照损伤小鼠的外周血细胞数量,极显著提高肝脏组织T-AOC、T-SOD活性和股骨骨髓DNA含量,极显著降低肝脏MDA含量,缓解了免疫器官胸腺的萎缩;且随着茶多糖浓度的增加,其抗辐射作用效果增强。综上,雅安藏茶茶多糖对~(60)Co-γ射线辐照损伤小鼠抗氧化功能和造血功能具有较强的防护作用,且具有一定的剂量效应。本研究结果为进一步深入探究茶多糖防辐射作用机理提供了新参考,也为雅安藏茶辐照保健功效研究提供了一定的理论依据。
In order to investigate the effect of tea polysaccharide on the radiation resistance of~(60)Co-γ ray radiation injury mice,tea polysaccharide extracted from Ya' an Tibetan tea was used as material,Mice were treated with different concentrations of tea polysaccharide for 15 days. On day 6,5Gy~(60)Co-γ ray was used to irradiate mice in each group,The total antioxidant capacity( T-AOC),total superoxide dismutase( T-SOD) activity and total protein( TP) content in the peripheral blood cells and liver tissues of each group were measured on the 16 th day after intragastric administration.The results showed that compared with the radiation group alone,the tea polysaccharides in Ya' an can significantly increase the number of peripheral blood cells in radiation-injured mice,the liver tissue T-AOC,T-SOD activity was significantly increased,the liver MDA content was significantly reduced,the femoral bone marrow DNA content was significantly increased and relieves the immune organ thymus atrophy,And with the increase of tea polysaccharide concentration,its anti-radiation effect is enhanced. In conclusion,the tea polysaccharides in Ya' an had a strong protective effect on the anti-oxidative function and hematopoietic function of mice irradiated by~(60)Co-γ ray radiation,and had a certain dose effect. The results of this study provide a new reference for further study on the mechanism of polysaccharide radiation,and also provide a theoretical basis for the study of radiation health care in Ya'an Tibetan tea.
In order to investigate the effect of tea polysaccharide on the radiation resistance of~(60)Co-γ ray radiation injury mice,tea polysaccharide extracted from Ya' an Tibetan tea was used as material,Mice were treated with different concentrations of tea polysaccharide for 15 days. On day 6,5Gy~(60)Co-γ ray was used to irradiate mice in each group,The total antioxidant capacity( T-AOC),total superoxide dismutase( T-SOD) activity and total protein( TP) content in the peripheral blood cells and liver tissues of each group were measured on the 16 th day after intragastric administration.The results showed that compared with the radiation group alone,the tea polysaccharides in Ya' an can significantly increase the number of peripheral blood cells in radiation-injured mice,the liver tissue T-AOC,T-SOD activity was significantly increased,the liver MDA content was significantly reduced,the femoral bone marrow DNA content was significantly increased and relieves the immune organ thymus atrophy,And with the increase of tea polysaccharide concentration,its anti-radiation effect is enhanced. In conclusion,the tea polysaccharides in Ya' an had a strong protective effect on the anti-oxidative function and hematopoietic function of mice irradiated by~(60)Co-γ ray radiation,and had a certain dose effect. The results of this study provide a new reference for further study on the mechanism of polysaccharide radiation,and also provide a theoretical basis for the study of radiation health care in Ya'an Tibetan tea.
引文
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[18]叶晓青,佘建明,贾新平,梁丽建,刘晓青.60Co-γ射线辐照对蕨类植物绿色球状体成苗的影响[J].核农学报,2016,30(5):835-840
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[23]黄洲,曾宪垠.不同剂量姜辣素对辐射损伤小鼠抗氧化功能和造血系统的防护作用[D].成都:四川农业大学,2010
[24]杨帆,李兴玉.茶多糖对小鼠骨髓造血细胞及免疫细胞的影响[J].现代生物医学进展,2009(12):2210-2213
[25]Choi J H,Kim D W,Yun N,Choi J S,Isiam M,Kim Y S,Lee S M.Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice[J].Journal of Natural Products,2011,74(5):1055-1060
[26]Geng Y,Du X,Cao X,Chen Y,Zheng H.The therapeutic effects of Zingiber officinale extract on mice irradiated by 60Co-ray[J].Journal of Medicinal Plants Research,2012,6(13):2590-2600
[27]关晨霞,高希布,梁杰.针灸对亚急性衰老小鼠脑组织中一氧化氮、丙二醛、超氧化物歧化酶的影响[J].针刺研究,2001,26(2):111-113
[28]石玉涛.茶多糖抗氧化和降血糖作用研究[D].武汉:华中农业大学,2010
[29]马作峰,姜瑞雪,张六通,王平.复合刺激对亚健康大鼠模型耐疲劳能力及血清SOD、MDA的影响[J].云南中医中药杂志2011,32(8):54-55
[30]王光义,蒋乃昌,贺志光.头针对脑梗塞患者血浆ET-1、MDA、NO的影响[J].中国针灸,2001,21(4):241-242
[2]刘树铮.医学放射生物学修订版[M].北京:原子能出版社,1998:10-11
[3]宛晓春.茶叶生物化学[M].第三版.北京:中国农业出版社,2008:27
[4]Yu F,Sheng J,Xu J,An X,Hu Q.Antioxidant activities ofcrude tea polyphenols,polysaccharides and proteins ofselenium-enriched tea and regular green tea[J].European Food Research and Technology,2007,225(5/6):843
[5]Wang Y,Yang Z,Wei X.Antioxidant activitiespotential of tea polysaccharide fractions obtained by ultrafiltration[J].International Journal of Biological Macromolecules,2012,50(3):558-564
[6]Wang Y,Zhao Y,Andrae-Marobela K,Okatch H,Xiao J.Tea polysaccharides as food antioxidants:an old woman’s tale?[J].Food Chemistry,2013,138(2):1923-1927
[7]陈建国,王茵,梅松,来伟旗,付颖,胡欣.茶多糖降血糖、改善糖尿病症状作用的研究[J].营养学报,2003,25(3):253-255
[8]王莉英,俞茂华,陈蔚.茶多糖对NOD小鼠1型糖尿病的预防作用[J].中华内分泌代谢杂志,2006,22(5):476-479
[9]芮莉莉,萧建中,程义勇.茶多糖对2型糖尿病小鼠降糖作用研究[J].中日友好医院学报,2005,19(2):93-96
[10]陈海霞,谢笔钧.茶多糖对小鼠实验性糖尿病的防治作用[J].营养学报,2002,24(1):85-86
[11]芮莉莉,萧建中,程义勇.茶多糖对2型糖尿病小鼠降糖作用研究[J].中日友好医院学报,2005,19(2):93-96
[12]段丽梅,孙红梅.不同剂量茶多糖补充对运动大鼠心肌、血清自由基代谢的影响[J].山东体育学院学报,2011,27(8):52-56
[13]张广惠,吴红英,周则卫,张晓东,王浩,刘培勋,龙伟.湖南黑茶对小鼠辐射损伤的保护作用[J].天然产物研究与开发,2016,28(5):775-780
[14]罗玲,周斌星,郭威,柴洁,李杨.普洱茶茶多糖的提取工艺的响应面分析研究[J].中国农学通报,2012,28(30):263-266
[15]陈宗道,周才琼,童华容.茶叶化学工程[M].重庆:西南师范大学出版社,1999
[16]陈昌辉,郭金龙,汪艳霞,杜晓,赵涛,赵正来.雅安藏茶制作工艺的品质变化[J].林产化学与工业,2011,31(4):69-74
[17]郭军,吴小说,刘廷国,黄劲松,汪新.60Co-γ辐照对红烧鸡块货架期及其感官品质的影响[J].核农学报,2016,30(3):502-508
[18]叶晓青,佘建明,贾新平,梁丽建,刘晓青.60Co-γ射线辐照对蕨类植物绿色球状体成苗的影响[J].核农学报,2016,30(5):835-840
[19]段雅彬,姚星辰,王财,陈湘宏,张娟玲,李向阳.黑果枸杞对X射线辐射小鼠的保护作用研究[J].天然产物研究与开发,2015,27(1):148-152
[20]周羽,谢振飞,耿艳艳,曾宪垠.姜辣素对60Coγ辐照损伤小鼠抗氧化功能和造血系统的防护作用[J].核技术,2011,34(9):658-662
[21]田琼,杨岚,张发科.血小板的四因子对小鼠急性放射损伤的防护作用与机理[J].中华放射医学与防护杂志,2000,20(6):416-418
[22]洪德志,张作法,蒋学,时连根.桑枝多糖对正常小鼠免疫功能的影响[J].蚕业科学,2011,37(3):481-485
[23]黄洲,曾宪垠.不同剂量姜辣素对辐射损伤小鼠抗氧化功能和造血系统的防护作用[D].成都:四川农业大学,2010
[24]杨帆,李兴玉.茶多糖对小鼠骨髓造血细胞及免疫细胞的影响[J].现代生物医学进展,2009(12):2210-2213
[25]Choi J H,Kim D W,Yun N,Choi J S,Isiam M,Kim Y S,Lee S M.Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice[J].Journal of Natural Products,2011,74(5):1055-1060
[26]Geng Y,Du X,Cao X,Chen Y,Zheng H.The therapeutic effects of Zingiber officinale extract on mice irradiated by 60Co-ray[J].Journal of Medicinal Plants Research,2012,6(13):2590-2600
[27]关晨霞,高希布,梁杰.针灸对亚急性衰老小鼠脑组织中一氧化氮、丙二醛、超氧化物歧化酶的影响[J].针刺研究,2001,26(2):111-113
[28]石玉涛.茶多糖抗氧化和降血糖作用研究[D].武汉:华中农业大学,2010
[29]马作峰,姜瑞雪,张六通,王平.复合刺激对亚健康大鼠模型耐疲劳能力及血清SOD、MDA的影响[J].云南中医中药杂志2011,32(8):54-55
[30]王光义,蒋乃昌,贺志光.头针对脑梗塞患者血浆ET-1、MDA、NO的影响[J].中国针灸,2001,21(4):241-242