雅安藏茶对~(60)Coγ辐射损伤小鼠的防护作用
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摘要
目的探究雅安藏茶对~(60)Coγ射线辐射损伤小鼠的防护作用。方法实验选取48只雄性SPF级小鼠,随机分为:正常对照、辐射对照、阳性对照,以及雅安藏茶低、中、高剂量组共6组。每组连续灌胃对应受试样品15 d,其中灌胃D6,除正常组外,其余组给予剂量为5Gy的~(60)Coγ射线一次性全身照射。灌胃结束后,眼睑采血、解剖,测定外周血细胞、血液抗氧化酶指标(SOD、CAT)、骨髓DNA含量、胸脾指数及小鼠肝脏组织总抗氧化指标(T-SOD、T-AOC)、TP、MDA等相关指标。结果与辐射对照相比,雅安藏茶中剂量组对辐射损伤小鼠的外周血细胞含量明显提高,其中,淋巴细胞(LYM)、白细胞(WBC)、血小板(PLT)含量分别极显著增加了44%、30%、36%(P<0.01),红细胞(RBC)、血红蛋白(HGB)含量分别显著增加了7%、6%(P<0.05),血液超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性分别极显著升高了67%、13%(P<0.01),肝脏总超氧化物歧化酶(T-SOD)、总抗氧化能力(T-AOC)活性分别极显著增高71%、17%(P<0.01),肝脏丙二醛(MDA)含量极显著下降了27%(P<0.01),小鼠体质量显著升高(P<0.05),股骨骨髓DNA含量极显著增高了26%(P<0.01),缓解脏器免疫器官的损伤。结论雅安藏茶对~(60)Coγ射线辐射损伤小鼠具有较强的防护作用,且具有一定的剂量效应。
Objective To explore the protective role of Ya'an Tibetan tea in mice irradiated by ~(60)Coγ ray.Methods Forty eight male SPF mice were randomly divided into 6 groups:normal control group,radiation control group,positive control group,Ya ‘an Tibetan tea low,medium and high dose groups.The mice were given the corresponding treatments for 15 days,and intragastric feeding for 6 days.Except the normal group,other groups were given whole body ~(60)Coγ ray irradiation at a dose of 5 Gy.Peripheral blood cells count and antioxidant indices(SOD,CAT),DNA contents in bone marrow,spleen index and antioxidant indexes(liver tissue T-SOD,T-AOC),TP,MDA and other related indicators were determined.Results Compared with the radiation control,cells in the peripheral blood were significantly higher in Ya'an Tibetan tea groups in radiation injury mice.The lymphocytes(LYM),white blood cells(WBC),platelet(PLT) content were significantly increased by 44%,30%,36%(P<0.01).Red blood cell(RBC),hemoglobin(HGB) content were increased by 7% and 6%(P<0.05).Blood superoxide dismutase(SOD),catalase(CAT) activities were significantly increased by 67% and 13%(P<0.01).Liver superoxide dismutase(T-SOD).total antioxidant capacity(T-AOC) were significantly increased by 71%,17%(P<0.01).Malondialdehyde(MDA) content was significantly decreased by 27%(P<0.01).Body weight gain was significantly increased(P<0.05).Bone marrow DNA content was significantly increased by 26%(P<0.01).Conclusion Ya ‘an Tibetan tea has protective effects on radiation injury in mice.
Objective To explore the protective role of Ya'an Tibetan tea in mice irradiated by ~(60)Coγ ray.Methods Forty eight male SPF mice were randomly divided into 6 groups:normal control group,radiation control group,positive control group,Ya ‘an Tibetan tea low,medium and high dose groups.The mice were given the corresponding treatments for 15 days,and intragastric feeding for 6 days.Except the normal group,other groups were given whole body ~(60)Coγ ray irradiation at a dose of 5 Gy.Peripheral blood cells count and antioxidant indices(SOD,CAT),DNA contents in bone marrow,spleen index and antioxidant indexes(liver tissue T-SOD,T-AOC),TP,MDA and other related indicators were determined.Results Compared with the radiation control,cells in the peripheral blood were significantly higher in Ya'an Tibetan tea groups in radiation injury mice.The lymphocytes(LYM),white blood cells(WBC),platelet(PLT) content were significantly increased by 44%,30%,36%(P<0.01).Red blood cell(RBC),hemoglobin(HGB) content were increased by 7% and 6%(P<0.05).Blood superoxide dismutase(SOD),catalase(CAT) activities were significantly increased by 67% and 13%(P<0.01).Liver superoxide dismutase(T-SOD).total antioxidant capacity(T-AOC) were significantly increased by 71%,17%(P<0.01).Malondialdehyde(MDA) content was significantly decreased by 27%(P<0.01).Body weight gain was significantly increased(P<0.05).Bone marrow DNA content was significantly increased by 26%(P<0.01).Conclusion Ya ‘an Tibetan tea has protective effects on radiation injury in mice.
引文
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[2]高月,马增春.辐射损伤防治药物发展历史与展望[J].辐射防护通讯,2009,29:30-35.
[3]刘河.低剂量电离辐射对人体的影响研究现状[J].中国城乡企业卫生,2012,27:107-108.
[4]姜爱丽,何煜波,胡文忠,等.雅安藏茶的工艺特点与传承发展[J].食品工业科技,2012,33:391-394.
[5]李解,陈雪皎,郭承义,等.雅安藏茶和低聚木糖复配物润肠通便作用[J].食品科学,2015,36:220-224.
[6]边金霖,郭金龙,李品武,等.雅安藏茶对脂肪酶的抑制作用[J].食品科学,2015,36:23-28.
[7]黄敏钧,陈卓,阮静雯,等.普洱茶抗辐射作用初探[J].广东茶业,2010(4):30-33.
[8]张广惠,吴红英,周则卫,等.湖南黑茶对小鼠辐射损伤的保护作用[J].天然产物研究与开发,2016,28:775-780.
[9]陈宗道,周才琼,童华容.茶叶化学工程[M].重庆:西南师范大学出版社,1999:35.
[10]段雅彬,姚星辰,王财,等.黑果枸杞对X射线辐射小鼠的保护作用研究[J].天然产物研究与开发,2015,27:148-152.
[11]周羽,谢振飞,耿艳艳,等.姜辣素对60Coγ辐照损伤小鼠抗氧化功能和造血系统的防护作用[J].核技术,2011,34:658-662.
[12]黄洲.不同剂量姜辣素对60Coγ辐射损伤小鼠抗氧化功能和造血系统的防护作用[D].四川农业大学,2010.
[13]田琼,杨岚,张发科.血小板的四因子对小鼠急性放射损伤的防护作用与机理[J].中华放射医学与防护杂志,2000,20:416-418.
[14]欧阳五庆.动物生理学[M].北京:科学出版社,2006:34.
[15]Liu L,Li Y,Fang E,et al.Microwave-assisted graft copolymerization ofε-caprolactone onto chitosan via the phthaloyl protection method[J].Carbohydr Poly,2005,60:351-356.
[16]谭德新,王艳丽,徐国财,等.复合交联剂型高吸水树脂的超声制备与性能研究[J].涂料工业,2009,39:41-44.
[17]刘廷国,陈艳,李斌,等.冻融循环对甲壳素凝聚态结构和脱乙酰反应的影响[J].农业机械学报,2009,40:140-145.
[18]刘茹,马森.茶多酚和茶多糖清除羟基自由基的效果[J].安徽农业科学,2012,40:1463-1464.
[19]周向军,高义霞,袁毅君,等.乌龙茶茶褐素提取工艺的优化及抗氧化研究[J].中国实验方剂学杂志,2011,17:36-40.