补肾药膳对PMOP模型大鼠PINP指标及骨组织形态的影响
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摘要
目的:观察补肾药膳对PMOP模型大鼠PINP指标及骨组织形态的影响。方法:SPF级雌性SD大鼠随机分为正常组、假手术组、模型空白组、高剂组、低剂量组、骨疏康组,每组10只。除正常组、假手术组外,其余大鼠进行卵巢摘除,适应性饲养15 d后,分组给药12周,ELISA法检测血清中骨代谢标志物PINP浓度表达情况,HE染色制成切片用医学图像分析系统软件测骨皮质厚度、骨小梁数量及平均间隔宽度。结果:模型空白组大鼠血清PINP浓度低于正常组(P<0.01);高剂量组、低剂量组、骨疏康组血清PINP浓度显著高于模型空白组(P<0.01),骨疏康组血清PINP浓度高于高剂量组、低剂量组,但差异无统计学意义(P>0.05);骨组织形态上,模型空白组显著差于正常组、假手术组(P<0.01),低剂量组、骨疏康组大鼠骨组织形态结构优于模型空白组(P<0.05)。结论:补肾药膳方能够有效地提升PMOP模型大鼠骨形成标志物水平,改善骨组织形态结构,促进骨形成,降低骨转化率,减少骨吸收,增加骨量,缓解骨质疏松。
Objective: To observe the effect of kidney-invigorating diet on PINP index and bone morphological changes in PMOP model rats. Methods: SPF female SD rats were randomly divided into normal group, sham operation group, model blank group, kidney-invigorating diet high dose group, low dose group and Gushukang(骨疏康) group, with 10 rats in each group. Except normal group and sham operation group, other rats were ovariectomized. After 15 days of adaptive feeding, the rats were divided into groups for 12 weeks. ELISA method was used to detect the expression of PINP in serum. HE staining was used to make medical images of slices though the system software to analyze the cortical bone thickness, trabecular number and average interval width.Results: Compared with the normal group, the level of PINP in model blank group was lower(P<0.01). Compared with model blank group, the serum PINP concentration in high dose group, low dose group and Gushukang group was significantly higher than that in model group(P<0.01). Although the Gushukang group was higher than the high-dose group and the low-dose group, there was no significant difference(P>0.05). The bone morphology of model blank group was significantly worse than that of normal group and sham operation group(P <0.01). The bone morphology of low dose group and Gushukang group was better than that of model blank group(P<0.05). Conclusion: Kidney-invigorating diet can effectively improve the level of bone formation markers,improve the morphological structure of bone tissue, promote bone formation, reduce bone turnover rate, reduce bone resorption, increase bone mass and relieve osteoporosis.
Objective: To observe the effect of kidney-invigorating diet on PINP index and bone morphological changes in PMOP model rats. Methods: SPF female SD rats were randomly divided into normal group, sham operation group, model blank group, kidney-invigorating diet high dose group, low dose group and Gushukang(骨疏康) group, with 10 rats in each group. Except normal group and sham operation group, other rats were ovariectomized. After 15 days of adaptive feeding, the rats were divided into groups for 12 weeks. ELISA method was used to detect the expression of PINP in serum. HE staining was used to make medical images of slices though the system software to analyze the cortical bone thickness, trabecular number and average interval width.Results: Compared with the normal group, the level of PINP in model blank group was lower(P<0.01). Compared with model blank group, the serum PINP concentration in high dose group, low dose group and Gushukang group was significantly higher than that in model group(P<0.01). Although the Gushukang group was higher than the high-dose group and the low-dose group, there was no significant difference(P>0.05). The bone morphology of model blank group was significantly worse than that of normal group and sham operation group(P <0.01). The bone morphology of low dose group and Gushukang group was better than that of model blank group(P<0.05). Conclusion: Kidney-invigorating diet can effectively improve the level of bone formation markers,improve the morphological structure of bone tissue, promote bone formation, reduce bone turnover rate, reduce bone resorption, increase bone mass and relieve osteoporosis.
引文
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[2]薛鹏,李玉坤.2017年版《原发性骨质疏松症诊疗指南》解读[J].河北医科大学学报,2018,39(1):1-6.
[3]郑洪新,刘伶,林庶茹,等.参茸补血丸对去卵巢大鼠骨质疏松防治作用的实验研究[J].中国老年学杂志,2006,3(3):385-388.
[4]许东晨.中医食疗对老年骨质疏松症患者临床症状和骨密度影响研究[J].中医临床研究,2014,6(33);101-102.
[5]林静,梁百慧,方森,等.食疗对肾虚型骨质疏松症作用的研究进展[J].护理研究,2017,31(13):1561-1563.
[6]郭文清.骨疏颗粒对骨质疏松大鼠骨代谢指标及炎症因子的影响[D].昆明:云南中医学院,2016.
[7] Hu W W,Zhang Z,He J W,et al. Establishing reference intervals for bone turnover markers in the healthy shanghai population and the relationship with bone mineral density in postmenopausal women[J]. Int J Endocrinol,2013:513925
[8] Gao C,Qiao J,Li SS,et al.The levels of bone turnover markers 25(OH)D and PTH and their relationship with bone mineral density in postmenopausal women in a sub-urban district in China[J]. Osteoporos Int,2017,12(28):211-218.
[9] KOIVUA LA M K,RISTELI L,RISTELI J. Measurement of aminoterminal propeptide of typeⅠvprocollagen(PINP)in serum[J].Clin Biochem,2012,45(12):920-927.
[10]刘康,史晓林.骨形态计量学在骨质疏松研究领域的研究进展[J].中国骨质疏松杂志,2007,13(6):440-442.
[11]江婷,方朝晖.中医药治疗骨质疏松症研究现状及进展[J].中医药临床志,2014,26(5):543-545.