奥拉西坦对血管性痴呆大鼠认知功能及脑组织N-myc表达的影响
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摘要
目的探讨奥拉西坦对血管性痴呆大鼠认知功能及脑组织N-myc表达的影响。方法将Wistar雄性大鼠分为假手术组10只、模型组10只和奥拉西坦治疗组10只,假手术组不进行模型建立操作,奥拉西坦治疗组腹腔注射10μg/kg奥拉西坦,模型组腹腔注射等量生理盐水,观察与检测大鼠认知功能及脑组织N-myc表达情况。结果模型组与奥拉西坦治疗组均成功建立了血管性痴呆大鼠模型,水迷宫实验显示奥拉西坦治疗组第3天、第5天逃避潜伏期明显低于模型组,差异有统计学意义(P<0.05)。奥拉西坦治疗组与模型组、假手术组的避暗实验潜伏期、错误次数比较差异均有统计学意义(P<0.05)。奥拉西坦治疗组大脑海马组织N-myc表达的吸光度明显高于模型组与假手术组,差异均有统计学意义(P<0.05)。结论奥拉西坦通过上调N-myc蛋白表达,可能对血管性痴呆大鼠海马神经元保护起着至关重要的作用,可作为治疗血管性痴呆的一个新途径。
Objective To investigate the effects of oxiracetam on brain tissue N-myc expression and nerve regeneration in the vascular dementia rats.Methods Male wistar rats were divided into sham operation group,model group and oxiracetam treatment group with 10 rats in each groups.The sham operation group was not performed model build operations,and the oxiracetam treatment group received oxiracetam by intraperitoneal injection of 10 μg/kg,the model group received intraperitoneal injection of saline.The rat brain N-myc expression was detected and the nerve regeneration organization was observed.Results Vascular dementia rat model were all successfully established in model group and the oxiracetam treatment group,the water maze test showed that the escape latency of the oxiracetam treatment group on day 3 and day 5 was obviously lower than that of the model group,and the result was statistically significant(P<0.05).There were statistically significant difference between oxiracetam treatment group and sham operation group,model group with respect to latency and error frequency(P<0.05).The absorbance of N-myc expression in hippocampus brain in the oxiracetam treatment group was significantly higher than that of model group and sham operation group(P<0.05).Conclusion Oxiracetam may play a crucial role in protection of hippocampal neurons in rats with vascular dementia through upregulation of N-myc protein expression,and may be a new way for the treatment of vascular dementia.
Objective To investigate the effects of oxiracetam on brain tissue N-myc expression and nerve regeneration in the vascular dementia rats.Methods Male wistar rats were divided into sham operation group,model group and oxiracetam treatment group with 10 rats in each groups.The sham operation group was not performed model build operations,and the oxiracetam treatment group received oxiracetam by intraperitoneal injection of 10 μg/kg,the model group received intraperitoneal injection of saline.The rat brain N-myc expression was detected and the nerve regeneration organization was observed.Results Vascular dementia rat model were all successfully established in model group and the oxiracetam treatment group,the water maze test showed that the escape latency of the oxiracetam treatment group on day 3 and day 5 was obviously lower than that of the model group,and the result was statistically significant(P<0.05).There were statistically significant difference between oxiracetam treatment group and sham operation group,model group with respect to latency and error frequency(P<0.05).The absorbance of N-myc expression in hippocampus brain in the oxiracetam treatment group was significantly higher than that of model group and sham operation group(P<0.05).Conclusion Oxiracetam may play a crucial role in protection of hippocampal neurons in rats with vascular dementia through upregulation of N-myc protein expression,and may be a new way for the treatment of vascular dementia.
引文
[1] 杨宏艳,韩云峰.奥拉西坦对血管性痴呆大鼠学习记忆的影响[J].中国老年学杂志,2016,36(16):3923-3924.
[2] 刘艳阳,刘佩军,雷德宝,等.奥拉西坦治疗脑梗死患者认知功能障碍的疗效观察[J].卒中与神经疾病,2016,23(3):204-206.
[3] ZHANG Q,YANG W,ZHANG Q,et al.Enantioselective HPLC determination of oxiracetam enantiomers and application to a pharmacokinetic study in beagle dogs[J].J Chromatogr B Analyt Technol Biomed Life Sci,2015,1(993/994):9-13.
[4] 张艳梅,李攀,朱润秀.GCs对慢性低灌注大鼠海马脑组织IL-2及KRT6A蛋白表达影响的实验研究[J].中风与神经疾病杂志,2016,33(10):909-911.
[5] WANG W,JI H,LI T,et al.Pharmacokinetic comparisons of S-oxiracetam and R-oxiracetam in beagle dogs[J].Acta Pharm,2016,66(2):279-287.
[6] 虞道锐,王涛,姬立平,等.高良姜提取物对糖尿病脑病大鼠认知功能障碍及海马病理改变的影响[J].海南医学院学报,2016,22(17):1929-1932.
[7] WANG C,LI F,GUAN Y,et al.Bone marrow stromal cells combined with oxiracetam influences the expression of B-cell lymphoma 2 in rats with ischemic stroke[J].J Stroke Cerebrovasc Dis,2014,23(10):2591-2597.
[8] WANG D,WU S.Development and validation of a RP-HPLC method for the simultaneous determination of aceglutamide and oxiracetam in an injection formulation[J].J Chromatogr Sci,2015,53(5):767-770.
[9] 李高申,郭梅珍,赵唯贤.逍遥散对阿尔茨海默病模型大鼠海马mRNA表达的影响[J].中国老年学杂志,2015,4(15):4199-4201.
[10] CHEN F,PENG J,LIU S,et al.The fluorescence and resonance rayleigh scattering spectra study on the interactions of palladium (II)-Nootropic chelate with Congo red and their analytical applications[J].Spectrochim Acta A Mol Biomol Spectrosc,2017,15(177):41-48.
[11] YAO X L,YAO Z H,LI L,et al.Oxiracetam can improve cognitive impairment after chronic cerebral hypoperfusion in rats[J].Psychiatry Res,2016,30(246):284-292.
[12] 张洋,白莉,顾镜月,等.奥拉西坦对新生大鼠缺氧缺血性脑损伤后AQP-4表达的影响[J].黑龙江医药科学,2015,38(3):80-81.
[13] 赵唯贤,李高申,王保伟,等.逍遥散对阿尔茨海默病大鼠海马CA3区PP-2 A、GSK-3β表达的影响[J].中国免疫学杂志,2014(5):623-626.
[14] BIAN H,HU Q,LIANG X,et al.Hyperbaric oxygen preconditioning attenuates hemorrhagic transformation through increasing PPARγ in hyperglycemic MCAO rats[J].Exp Neurol,2015,265(2):22-29.
[15] GUPTA R K,PRASAD S.Age-dependent alterations in the interactions of NF-κB and N-myc with GLT-1/EAAT2 promoter in the pericontusional cortex of mice subjected to traumatic brain injury[J].Mol Neurobiol,2016,53(5):3377-3388.
[16] 夏翠萍,陈进,沈丽华.奥拉西坦对脑梗死大鼠细胞凋亡及神经再生的影响[J].南通大学学报(医学版),2014,12(3):180-182.
[17] WANG F,ZHONG H,LI X,et al.Electroacupuncture attenuates reference memory impairment associated with astrocytic NDRG2 suppression in APP/PS1 transgenic mice[J].Mol Neurobiol,2014,50(2):305-313.
[18] SAHIN P,MCCAIG C,JEEVAHAN J,et al.The cell survival kinase SGK1 and its targets FOXO3a and NDRG1 in aged human brain[J].Neuropathol Appl Neurobiol,2013,39(6):623-633.
[19] 赵玉娇,马风杰,李芳,等.奥拉西坦对大鼠脑缺血再灌注损伤Bcl-2及 Bax表达的影响[J].辽宁医学院学报,2014,13(2):1-2;26.
[20] CHEN J,ZHOU S N,ZHANG Y M,et al.Glycosides of cistanche improve learning and memory in the rat model of vascular dementia[J].Eur Rev Med Pharmacol Sci,2015,19(7):1234-1240.
[21] ZHANG Q,YANG W,YANG Y,et al.Comparative pharmacokinetic studies of racemic oxiracetam and its pure enantiomers after oral administration in rats by a stereoselective HPLC method[J].J Pharm Biomed Anal,2015,8(111):153-158.
[2] 刘艳阳,刘佩军,雷德宝,等.奥拉西坦治疗脑梗死患者认知功能障碍的疗效观察[J].卒中与神经疾病,2016,23(3):204-206.
[3] ZHANG Q,YANG W,ZHANG Q,et al.Enantioselective HPLC determination of oxiracetam enantiomers and application to a pharmacokinetic study in beagle dogs[J].J Chromatogr B Analyt Technol Biomed Life Sci,2015,1(993/994):9-13.
[4] 张艳梅,李攀,朱润秀.GCs对慢性低灌注大鼠海马脑组织IL-2及KRT6A蛋白表达影响的实验研究[J].中风与神经疾病杂志,2016,33(10):909-911.
[5] WANG W,JI H,LI T,et al.Pharmacokinetic comparisons of S-oxiracetam and R-oxiracetam in beagle dogs[J].Acta Pharm,2016,66(2):279-287.
[6] 虞道锐,王涛,姬立平,等.高良姜提取物对糖尿病脑病大鼠认知功能障碍及海马病理改变的影响[J].海南医学院学报,2016,22(17):1929-1932.
[7] WANG C,LI F,GUAN Y,et al.Bone marrow stromal cells combined with oxiracetam influences the expression of B-cell lymphoma 2 in rats with ischemic stroke[J].J Stroke Cerebrovasc Dis,2014,23(10):2591-2597.
[8] WANG D,WU S.Development and validation of a RP-HPLC method for the simultaneous determination of aceglutamide and oxiracetam in an injection formulation[J].J Chromatogr Sci,2015,53(5):767-770.
[9] 李高申,郭梅珍,赵唯贤.逍遥散对阿尔茨海默病模型大鼠海马mRNA表达的影响[J].中国老年学杂志,2015,4(15):4199-4201.
[10] CHEN F,PENG J,LIU S,et al.The fluorescence and resonance rayleigh scattering spectra study on the interactions of palladium (II)-Nootropic chelate with Congo red and their analytical applications[J].Spectrochim Acta A Mol Biomol Spectrosc,2017,15(177):41-48.
[11] YAO X L,YAO Z H,LI L,et al.Oxiracetam can improve cognitive impairment after chronic cerebral hypoperfusion in rats[J].Psychiatry Res,2016,30(246):284-292.
[12] 张洋,白莉,顾镜月,等.奥拉西坦对新生大鼠缺氧缺血性脑损伤后AQP-4表达的影响[J].黑龙江医药科学,2015,38(3):80-81.
[13] 赵唯贤,李高申,王保伟,等.逍遥散对阿尔茨海默病大鼠海马CA3区PP-2 A、GSK-3β表达的影响[J].中国免疫学杂志,2014(5):623-626.
[14] BIAN H,HU Q,LIANG X,et al.Hyperbaric oxygen preconditioning attenuates hemorrhagic transformation through increasing PPARγ in hyperglycemic MCAO rats[J].Exp Neurol,2015,265(2):22-29.
[15] GUPTA R K,PRASAD S.Age-dependent alterations in the interactions of NF-κB and N-myc with GLT-1/EAAT2 promoter in the pericontusional cortex of mice subjected to traumatic brain injury[J].Mol Neurobiol,2016,53(5):3377-3388.
[16] 夏翠萍,陈进,沈丽华.奥拉西坦对脑梗死大鼠细胞凋亡及神经再生的影响[J].南通大学学报(医学版),2014,12(3):180-182.
[17] WANG F,ZHONG H,LI X,et al.Electroacupuncture attenuates reference memory impairment associated with astrocytic NDRG2 suppression in APP/PS1 transgenic mice[J].Mol Neurobiol,2014,50(2):305-313.
[18] SAHIN P,MCCAIG C,JEEVAHAN J,et al.The cell survival kinase SGK1 and its targets FOXO3a and NDRG1 in aged human brain[J].Neuropathol Appl Neurobiol,2013,39(6):623-633.
[19] 赵玉娇,马风杰,李芳,等.奥拉西坦对大鼠脑缺血再灌注损伤Bcl-2及 Bax表达的影响[J].辽宁医学院学报,2014,13(2):1-2;26.
[20] CHEN J,ZHOU S N,ZHANG Y M,et al.Glycosides of cistanche improve learning and memory in the rat model of vascular dementia[J].Eur Rev Med Pharmacol Sci,2015,19(7):1234-1240.
[21] ZHANG Q,YANG W,YANG Y,et al.Comparative pharmacokinetic studies of racemic oxiracetam and its pure enantiomers after oral administration in rats by a stereoselective HPLC method[J].J Pharm Biomed Anal,2015,8(111):153-158.