不同侧根密度对水稻生长发育及主要农艺性状的影响
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摘要
RM109是用化学诱变剂(NaN_3)处理粳稻品种大力(Oryza sativa L. cv. Oochikara)得到的具有2,4-D抗性的无侧根突变体,本研究以RM109与侧根正常的籼型常规水稻‘密阳23’杂交后代F_4群体为研究对象,根据侧根数目从无到有,从少到多把F_4群体分成7个区组LR1～LR7,分别研究了不同侧根密度对水稻主要农艺性状的影响以及相关性,同时分析了不同侧根密度水稻植株株高分蘖数的动态变化过程。研究结果表明,水稻不同侧根密度之间,冠根的根粗没有显著差异;水稻不同侧根密度之间,根长有略微的差异,LR4与LR3存在显著差异;水稻不同侧根密度之间,株高与分蘖数存在显著差异,说明水稻侧根的存在对株高和分蘖的生长发育起着重要的作用;水稻不同侧根密度众多农艺性状的相关性分析表明,侧根密度与株高、单株有效分蘖数、单株生物产量之间存在极显著的正相关关系,与稻米的外观品质、粘度值无相关性。
The no-lateral-root mutant RM109 was obtained from Oryza sativa L. cv. Oochikara treated by NaN_3,which was resistant to 2,4-D. In this study, the cross F_4 populations of the mutant RM109 and "Milyang23" with normal lateral roots were used as the research objects, which were divided into 7 groups LR1～LR7 according to the number of lateral roots. The effects of different lateral root densities on growth, development and main agronomic characters of rice were analyzed, and the dynamic variation of high tiller number of rice plants with different lateral root densities was investigated as well. The results showed that the width of crown roots had no significant difference between different lateral root densities. The root length of different lateral root densities had slight difference, and the differences between LR3 and LR4 were significant. The plant height and tiller number had significant difference, indicating that the lateral root took important part in plant height and tiller development.The correlation analysis of the agronomic traits of different lateral root densities indicated that the lateral root density had an extremely significant positive correlation with plant height, tiller number per plant and biomass per plant, but had no relation with appearance quality and starch viscosity of rice.
The no-lateral-root mutant RM109 was obtained from Oryza sativa L. cv. Oochikara treated by NaN_3,which was resistant to 2,4-D. In this study, the cross F_4 populations of the mutant RM109 and "Milyang23" with normal lateral roots were used as the research objects, which were divided into 7 groups LR1～LR7 according to the number of lateral roots. The effects of different lateral root densities on growth, development and main agronomic characters of rice were analyzed, and the dynamic variation of high tiller number of rice plants with different lateral root densities was investigated as well. The results showed that the width of crown roots had no significant difference between different lateral root densities. The root length of different lateral root densities had slight difference, and the differences between LR3 and LR4 were significant. The plant height and tiller number had significant difference, indicating that the lateral root took important part in plant height and tiller development.The correlation analysis of the agronomic traits of different lateral root densities indicated that the lateral root density had an extremely significant positive correlation with plant height, tiller number per plant and biomass per plant, but had no relation with appearance quality and starch viscosity of rice.
引文
Cai K.Z.,Luo S.M.,and Duan S.S.,2003,The relationship between spatial distribution of rice root system and yield,Huanan Nongye Daxue Xuebao(Journal of South China Agricultural University),24(3):1-4(蔡昆争,骆世明,段舜山,2003,水稻根系的空间分布及其与产量的关系,华南农业大学学报,24(3):1-4)
Cai Z.Z.,Chen X.Q.,Tang W.Q.,Li W.Q.,Wu W.R.,and Duan Y.L.,2016,Morphological characteristics,genetic analysis and gene location of two short panicle mutants of rice,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1183-1188(蔡正正,陈学群,唐唯其,李文强,吴为人,段远霖,2016,两种水稻短穗突变体的形态特征,遗传分析和基因定位,基因组学与应用生物学,35(5):1183-1188)
Chen W.F.,Xu Z.J.,Zhang W.Z.,Zhang L.B.,and Yang S.R.,2001,Creation of new plant type and breeding rice for super high yield,Zuowu Xuebao(Acta Agronomica Sinica),27(5):665-672(陈温福,徐正进,张文忠,张龙步,杨守仁,2001,水稻新株型创造与超高产育种,作物学报,27(5):665-672)
Chhun T.,Taketa S.,Tsurumi S.,and Ichiii M.,2003,The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Lrt1 of rice(Oryza sativa L.),Plant Growth Regul.,39(2):161-170
Hao Z.B.,Cang J.,Xu Z.,Gao J.G.,and Ichii M.,2001,Agronomic characteristics from the RM109 lateral root mutant of rice,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),32(1):75-79(郝再彬,苍晶,徐仲,高继国,一井真比古,2001,水稻侧根突变体RM109的农艺性状,东北农业大学学报,32(1):75-79)
Kitomi Y.,Inahashi H.,Takehisa H.,Sato Y.,and Inukai Y.,2012,Os IAA13-mediated auxin signaling is involved in lateral root initiation in rice,Plant Sci.,190(3):116-122
Liu D.T.,Jing Y.P.,Chen J.J.,Yu X.R.,and Wang Z.,2014,Rice lateral root development and its impact factors,Zuowu Xuebao(Acta Agronomica Sinica),40(8):1403-1411(刘大同,荆彦平,陈晶晶,余徐润,王忠,2014,水稻的侧根发育及其影响因素,作物学报,40(8):1403-1411)
Liu D.T.,Jing Y.P.,Li D.L.,Yu X.R.,and Wang Z.,2013,Research advances in plant lateral root development,Zhiwu Shengli Xuebao(Plant Physiology Journal),49(11):1127-1137(刘大同,荆彦平,李栋梁,余徐润,王忠,2013,植物侧根发育的研究进展,植物生理学报,49(11):1127-1137)
Liu T.J.,Qi C.H.,and Tang J.J.,2002,Studies on relationship between the character of root and yield formation in rice,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),35(11):1416-1419(刘桃菊,戚昌瀚,唐建军,2002,水稻根系建成与产量及其构成关系的研究,中国农业科学,35(11):1416-1419)
Pan X.H.,Wang Y.R.,and Fu J.R.,1996,Advance in the study on the growth-physiology in rice of root system(Oryaza sativa),Zhiwuxue Tongbao(Chinese Bulletin of Botany),13(2):13-20(潘晓华,王永锐,傅家瑞,1996,水稻根系生长生理的研究进展,植物学通报,3(2):13-20)
Wang H.,Taketa S.,Miyao A.,Hirochika H.,and Ichii M.,2006,Isolation of a novel lateral-rootless mutant in rice(Oryza stative L.)with reduced sensitivity to auxin,Plant Sci.,170(1):70-77
Xing G.F.,Feng W.J.,Niu X.L.,Zhang C.L.,Ma J.H.,and Guo P.Y.,2015,Physiological mechanisms in phytohormone regulation of plant lateral root development,Zhiwu Shengli Xuebao(Plant Physiology Journal),51(12):2101-2108(邢国芳,冯万军,牛旭龙,张春来,马金虎,郭平毅,2015,植物激素调控侧根发育的生理机制,植物生理学报,51(12):2101-2108)
Yang R.F.,Bai J.J.,Fang J.,Zeng W.,Li G.X.,and Piao Z.Z.,2017,Comparative analysis on main quality characters and starch chain length distribution between insect-resistant transgenic rice Xiushui 134-Bt and its non-transgenic parent(Oryza sativa L.),Shanghai Nongye Xuebao(Acta Agriculturae Shanghai),33(1):31-34(杨瑞芳,白建江,方军,曾威,李刚燮,朴钟泽,2017,转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较,上海农业学报,33(1):31-34)
Yu L.F.,Hao Z.B.,and Cang J.,2005,Observation on microstructures and root hair morphology of root mutant RM109 of rice(Oryza sativa cv.Oochikara),Zhiwu Shenglixue Tongxun(China Academic Journal Electronic),41(3):328-330(于龙凤,郝再彬,苍晶,2005,水稻无侧根突变体RM109的显微结构及其根毛形态观察,植物生理学通讯,41(3):328-330)
Zhang H.,Xue Y.G.,Wang Z.Q.,Yang J.C.,and Zhang J.H.,2009,Morphological and physiological traits of roots and their relationships with shoot growth in super rice,Field Crops Res.,113(1):31-40
Zheng W.J.,Chen H.M.,Ye J.,Zhu S.H.,and Ding W.N.,2016,Genetic analysis and gene mapping of a short root mutant Osksr5 in rice(Oryza sativa L.),Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),36(12):2405-2410(郑文娟,陈红梅,叶金,朱世华,丁沃娜,2016,水稻短根突变体Osksr5的遗传分析和基因定位,西北植物学报,36(12):2405-2410)
Zhu Z.X.,Liu Y.,Liu S.J.,Mao C.Z.,Wu Y.R.,and Wu P.,2012,A gain-of-function mutation in OsIAA11 affects lateral root development in rice,Mol.Plant,5(1):154-161
Cai Z.Z.,Chen X.Q.,Tang W.Q.,Li W.Q.,Wu W.R.,and Duan Y.L.,2016,Morphological characteristics,genetic analysis and gene location of two short panicle mutants of rice,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1183-1188(蔡正正,陈学群,唐唯其,李文强,吴为人,段远霖,2016,两种水稻短穗突变体的形态特征,遗传分析和基因定位,基因组学与应用生物学,35(5):1183-1188)
Chen W.F.,Xu Z.J.,Zhang W.Z.,Zhang L.B.,and Yang S.R.,2001,Creation of new plant type and breeding rice for super high yield,Zuowu Xuebao(Acta Agronomica Sinica),27(5):665-672(陈温福,徐正进,张文忠,张龙步,杨守仁,2001,水稻新株型创造与超高产育种,作物学报,27(5):665-672)
Chhun T.,Taketa S.,Tsurumi S.,and Ichiii M.,2003,The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Lrt1 of rice(Oryza sativa L.),Plant Growth Regul.,39(2):161-170
Hao Z.B.,Cang J.,Xu Z.,Gao J.G.,and Ichii M.,2001,Agronomic characteristics from the RM109 lateral root mutant of rice,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),32(1):75-79(郝再彬,苍晶,徐仲,高继国,一井真比古,2001,水稻侧根突变体RM109的农艺性状,东北农业大学学报,32(1):75-79)
Kitomi Y.,Inahashi H.,Takehisa H.,Sato Y.,and Inukai Y.,2012,Os IAA13-mediated auxin signaling is involved in lateral root initiation in rice,Plant Sci.,190(3):116-122
Liu D.T.,Jing Y.P.,Chen J.J.,Yu X.R.,and Wang Z.,2014,Rice lateral root development and its impact factors,Zuowu Xuebao(Acta Agronomica Sinica),40(8):1403-1411(刘大同,荆彦平,陈晶晶,余徐润,王忠,2014,水稻的侧根发育及其影响因素,作物学报,40(8):1403-1411)
Liu D.T.,Jing Y.P.,Li D.L.,Yu X.R.,and Wang Z.,2013,Research advances in plant lateral root development,Zhiwu Shengli Xuebao(Plant Physiology Journal),49(11):1127-1137(刘大同,荆彦平,李栋梁,余徐润,王忠,2013,植物侧根发育的研究进展,植物生理学报,49(11):1127-1137)
Liu T.J.,Qi C.H.,and Tang J.J.,2002,Studies on relationship between the character of root and yield formation in rice,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),35(11):1416-1419(刘桃菊,戚昌瀚,唐建军,2002,水稻根系建成与产量及其构成关系的研究,中国农业科学,35(11):1416-1419)
Pan X.H.,Wang Y.R.,and Fu J.R.,1996,Advance in the study on the growth-physiology in rice of root system(Oryaza sativa),Zhiwuxue Tongbao(Chinese Bulletin of Botany),13(2):13-20(潘晓华,王永锐,傅家瑞,1996,水稻根系生长生理的研究进展,植物学通报,3(2):13-20)
Wang H.,Taketa S.,Miyao A.,Hirochika H.,and Ichii M.,2006,Isolation of a novel lateral-rootless mutant in rice(Oryza stative L.)with reduced sensitivity to auxin,Plant Sci.,170(1):70-77
Xing G.F.,Feng W.J.,Niu X.L.,Zhang C.L.,Ma J.H.,and Guo P.Y.,2015,Physiological mechanisms in phytohormone regulation of plant lateral root development,Zhiwu Shengli Xuebao(Plant Physiology Journal),51(12):2101-2108(邢国芳,冯万军,牛旭龙,张春来,马金虎,郭平毅,2015,植物激素调控侧根发育的生理机制,植物生理学报,51(12):2101-2108)
Yang R.F.,Bai J.J.,Fang J.,Zeng W.,Li G.X.,and Piao Z.Z.,2017,Comparative analysis on main quality characters and starch chain length distribution between insect-resistant transgenic rice Xiushui 134-Bt and its non-transgenic parent(Oryza sativa L.),Shanghai Nongye Xuebao(Acta Agriculturae Shanghai),33(1):31-34(杨瑞芳,白建江,方军,曾威,李刚燮,朴钟泽,2017,转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较,上海农业学报,33(1):31-34)
Yu L.F.,Hao Z.B.,and Cang J.,2005,Observation on microstructures and root hair morphology of root mutant RM109 of rice(Oryza sativa cv.Oochikara),Zhiwu Shenglixue Tongxun(China Academic Journal Electronic),41(3):328-330(于龙凤,郝再彬,苍晶,2005,水稻无侧根突变体RM109的显微结构及其根毛形态观察,植物生理学通讯,41(3):328-330)
Zhang H.,Xue Y.G.,Wang Z.Q.,Yang J.C.,and Zhang J.H.,2009,Morphological and physiological traits of roots and their relationships with shoot growth in super rice,Field Crops Res.,113(1):31-40
Zheng W.J.,Chen H.M.,Ye J.,Zhu S.H.,and Ding W.N.,2016,Genetic analysis and gene mapping of a short root mutant Osksr5 in rice(Oryza sativa L.),Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),36(12):2405-2410(郑文娟,陈红梅,叶金,朱世华,丁沃娜,2016,水稻短根突变体Osksr5的遗传分析和基因定位,西北植物学报,36(12):2405-2410)
Zhu Z.X.,Liu Y.,Liu S.J.,Mao C.Z.,Wu Y.R.,and Wu P.,2012,A gain-of-function mutation in OsIAA11 affects lateral root development in rice,Mol.Plant,5(1):154-161