基于无机元素谱的玉竹药材质量特征研究
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摘要
目的:建立不同产地玉竹药材无机元素谱,并结合微量元素组学对不同品质药材样本中26种无机元素的含量进行比较分析。方法:样品经微波消解后,采用ICP-MS法测定无机元素的量,绘制出无机元素谱,并用SPSS 16. 0,MVSP软件对数据进行主成分分析。结果:元素Hg,Al,Cr的量较高,表明有害金属元素的量应引起关注;不同产地玉竹药材中各元素呈相似的分布态势,表明所建立的无机元素谱具有一定的特征性,可作为鉴定玉竹药材的参考依据;通过主成分分析(PCA),比较不同样品中无机元素含量的差异,可将不同采收期及不同加工方法的玉竹药材区分开来,并筛选出Fe,V,Al,Cd,As,Na,Hg是玉竹药材的特征无机元素。结论:通过建立不同产地玉竹药材无机元素谱,为鉴定玉竹品种提供参考依据;同时分析各元素的含量,结合统计学方法,比较不同采收期及加工方法的玉竹药材中无机元素含量的差异,为其质量控制、品质评价及安全性研究提供依据。
Objective: To explore the character of inorganic elements in Polygonatum odoratum and compare the contents of 26 inorganic elements in Polygonatum odoratum between different origin by trace elements omics. Methods: The sample solutions were analyzed by ICP-MS after microwave digestion and established the fingerprint of inorganic elements. The data of principal components and cluster were analyzed with the SPSS 16. 0,MVSP software. Results: The contents of Hg,Al and Cr were abundant. So the content of heavy metals and harmful elements should be caused for concern. The inorganic elements in Polygonatum odoratum from different areas displayed similar element spectrum. The characteristics of inorganic elements fingerprint could be used in resources survey of Polygonatum odoratum. Eight main factors were selected by principal component analysis(PCA). The PCA results showed that Fe,V,Al,Cd,As,Na and Hg were the characteristic elements of Polygonatum odoratum,which could be the most important factors affecting of Polygonatum odoratum between different origin. Conclusion:The fingerprint of inorganic elements provides the evidence for the resources survey in Polygonatum odoratum. The content of inorganic elements in Polygonatum odoratum shows characteristic element spectrum distribution and significant differences among samples based on the multivariate statistical analysis. This experiment provides the evidence for the quality control and safety evaluation of Polygonatum odoratum.
Objective: To explore the character of inorganic elements in Polygonatum odoratum and compare the contents of 26 inorganic elements in Polygonatum odoratum between different origin by trace elements omics. Methods: The sample solutions were analyzed by ICP-MS after microwave digestion and established the fingerprint of inorganic elements. The data of principal components and cluster were analyzed with the SPSS 16. 0,MVSP software. Results: The contents of Hg,Al and Cr were abundant. So the content of heavy metals and harmful elements should be caused for concern. The inorganic elements in Polygonatum odoratum from different areas displayed similar element spectrum. The characteristics of inorganic elements fingerprint could be used in resources survey of Polygonatum odoratum. Eight main factors were selected by principal component analysis(PCA). The PCA results showed that Fe,V,Al,Cd,As,Na and Hg were the characteristic elements of Polygonatum odoratum,which could be the most important factors affecting of Polygonatum odoratum between different origin. Conclusion:The fingerprint of inorganic elements provides the evidence for the resources survey in Polygonatum odoratum. The content of inorganic elements in Polygonatum odoratum shows characteristic element spectrum distribution and significant differences among samples based on the multivariate statistical analysis. This experiment provides the evidence for the quality control and safety evaluation of Polygonatum odoratum.
引文
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[2]晏春耕,曹瑞芳.玉竹的研究进展与开发利用[J].中国现代中药,2007,9(4):33-37.
[3]才巨明,孙涛,张文军,等.我国玉竹的栽培研究进展[J].人参研究,2012,4(4):55-57.
[4]李妙然,秦灵灵,魏颖,等.玉竹化学成分与药理作用研究进展[J].中华中医药学刊,2015,33(8):1939-1943.
[5]肖凤艳,奚广生. 2年生栽培关玉竹的多糖与矿质元素含量测定[J].贵州农业科学,2011,39(1):61-63.
[6]赵杨迪,潘远智,刘碧英.玉竹对土壤Cd Pb的吸收和耐性研究[J].农业环境科学学报,2010,29(11):2087-2093.
[7]张忠诚,徐祗云,张素洁.镁与人体健康[J].微量元素与健康研究,2006,23(4):67-69.
[8]孙晓莉,刘灿,董顺福,等.芦荟茶及其茶水中常量和微量元素的测定分析[J].微量元素与健康研究,2003,20(3):33-35.
[9]刘金香.生命元素镁保护心脏的功能及其机理[J].微量元素与健康研究,2001,18(4):75-78.
[10]龙文韬,李婷婷,劳远司.分光光度法测定瓜果蔬菜中铁的含量[J].微量元素与健康研究,2004,21(5):34-36.
[11]刘伟明,冷红霞,刘巨涛,等.各种脑活素中微量元素的比较[J].第四军医大学学报,2001,22(7):587-589.
[12]董顺福,韩丽琴,朱志国,等.治疗心血管疾病中成药物中无机元素模糊聚类分析[J].中成药,2003,25(4):316-318.
[13]李金英,石磊,鲁盛会,等.电感耦合等离子体质谱(ICPMS)及其联用技术研究进[J].中国无机分析化学,2012,2(2):1-5.
[14]聂西度,梁逸曾,符靓,等.电感耦合等离子体质谱法测定甜味剂中重金属元素[J].光谱学与光谱分析,2012,32(10):2838-2841.
[15]聂黎行,刘燕,王钢力,等. ICP-MS直接测定中药参麦注射液中26种元素[J].中国药学杂志,2011,46(11):866-868.
[16]赵一懿,郭洪祝,傅欣彤,等.基于ICP-MS法分析银杏叶系列品种中25种无机元素[J].中草药,2017,48(10):1991-1997.
[17]国家药典委员会.中华人民共和国药典[S]. 2015年版.一部.北京:中国医药科技出版社,2015:205-207.
[18]马临科,方翠芬,祝明.中国药典2015年版中药中有害物残留检测的质量控制新动向[J].中国现代应用药学,2016,33(5):608-611.