摘要
从患病的半滑舌鳎(Cynoglossus semilaevis)溃疡体表处筛选到一株H1B6优势菌株,根据形态观察、16S rDNA检测、生理生化鉴定结果,H1B6菌株与溶藻弧菌最为接近,而在以16S rDNA序列构建的系统发育进化树模型中,H1B6菌株也与溶藻弧菌菌株聚为一支。Biolog微生物鉴定结果显示,H1B6菌株为溶藻弧菌的可能性为98.6%,确定H1B6菌株为溶藻弧菌。斑马鱼攻毒试验结果显示,H1B6菌株24 h的半数致死浓度(LD_(50))为4.58×10~6 CFU/mL。
A dominant strain of H1B6 was isolated from the ulcer surface of diseased Cynoglossus semilaevis. According to morphological observation, 16 S rDNA sequencing, physiological and biochemical identification results, H1B6 strain was suspected as Vibrio alginolyticus. According to the phylogenetic tree model constructed by 16 S rDNA sequence, H1B6 strain was clustered with Vibrio alginolyticus strains. Furthermore, H1B6 strain was finally identified as Vibrio alginolyticus by using Biolog microbial identification test,and the probability(PROB value) was 98.6%. H1B6 strain's acute toxicity test on zebrafish was performed and half lethal concentration(LD_(50)) after 24 hours was determined as 4.58×10~6 CFU/mL.
引文
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