跑台运动对阿尔茨海默病模型小鼠学习记忆能力的影响
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摘要
目的探讨跑台运动对阿尔茨海默病模型小鼠学习记忆能力的影响及其机制。方法 32只小鼠随机分为正常组、模型组、运动1组(E1组)、运动2组(E2组)。每天腹腔注射120 mg/kg D-半乳糖和100 mg/kg亚硝酸钠造模,连续8周。E1、E2组每天以10 m/s分别跑100、200 m。第9周采用Morris水迷宫检测小鼠学习记忆能力,并取大脑海马测定总巯基、乙酰胆碱酯酶(Ach E)、谷胱甘肽过氧化物酶。结果与模型组相比,E2组小鼠空间学习记忆能力提高(P<0.05);正常组、E1、E2组海马组织中总巯基含量及正常组、E1组Ach E含量升高(P<0.01或0.05)。结论跑台运动能改善阿尔茨海默病模型小鼠学习记忆能力、海马中抗氧化及胆碱能活性。
Objective To study the effect and mechanism of treadmill exercise on learning and memory ability(LMA) in mice with Alzheimer's disease(AD). Methods Thirty-two mice were randomized to normal, model, E1 and E2 groups. AD model was established by daily intraperitoneal injection of 120 mg/kg D-galactose and 100 mg/kg sodium nitrite for 8 weeks. E1 and E2 groups were treated with daily treadmill exercise at a rate of 10 m/s for 100 and 200 meters, respectively. At week 9, LMA was determined by Morris water maze, and levels of hydrosulfuryl, acetylcholine esterase(AchE), and glutathione peroxidase were also detected in the cerebral hippocampus. Results Compared with model group, LMA was enhanced in E2 group(P<0.01); hydrosulfuryl content in normal, E1 and E2 groups, and Ach E level in normal and E1 groups were increased(P<0.01 or 0.05). Conclusion The treadmill exercise can improve the LMA and antioxidant and cholinergic activity in the hippocampus of AD mice.
Objective To study the effect and mechanism of treadmill exercise on learning and memory ability(LMA) in mice with Alzheimer's disease(AD). Methods Thirty-two mice were randomized to normal, model, E1 and E2 groups. AD model was established by daily intraperitoneal injection of 120 mg/kg D-galactose and 100 mg/kg sodium nitrite for 8 weeks. E1 and E2 groups were treated with daily treadmill exercise at a rate of 10 m/s for 100 and 200 meters, respectively. At week 9, LMA was determined by Morris water maze, and levels of hydrosulfuryl, acetylcholine esterase(AchE), and glutathione peroxidase were also detected in the cerebral hippocampus. Results Compared with model group, LMA was enhanced in E2 group(P<0.01); hydrosulfuryl content in normal, E1 and E2 groups, and Ach E level in normal and E1 groups were increased(P<0.01 or 0.05). Conclusion The treadmill exercise can improve the LMA and antioxidant and cholinergic activity in the hippocampus of AD mice.
引文
[1]Friedland R P,Fritsch T,Smyth K A,et al.Patients with Alzheimer’s disease have reduced activitives in midlife compared with healthy control-group members[J].Proc Natl Aead Sci USA,2001,98(6):3440-3445.
[2]高谦,吴宗耀,姚志彬,等.短期和长期训练对小鼠脑局灶缺血后大脑皮质胆碱能纤维密度的影响[J].中国临床康复,2006,10(34):61-63.
[3]高谦,吴宗耀,姚志彬,等.运动训练对小鼠脑局灶缺血后大脑皮质胆碱能纤维密度的作用[J].中国临床康复,2003,7(16):2274-2275.
[4]陈运才,姚志彬,顾耀铭,等.运动对小鼠海马结构和大脑皮质胆碱能纤维的影响[J].神经解剖学杂志,1994,10(1):11-16.
[5]Tanka K,Dequadrosjr A C,Santos R F,et al.Benefits of physical execise on executive function in older people with Parkinson’s disease[J].Brain Cognition,2009,69(2):435-441.
[6]van Praag H,Shubert T,Zhao C,et al.Exercise enhances learning and hippocampal neurogenesis in agedmice[J].Neurosci,2005,25(38):8680-8685.
[7]Uysal N,Tuqyan K,Kayatekin B,et al.The effect of regular aerobic exercise in adolescent period on hippocampal neuron density,apoptosis and spatial memory[J].Neurosci Letters,2005,383:241-245
[8]Shang Y Z,gong M Y,Zhou X X,et al.Improving effects of SSF on memory deficits and pathological changes of neural and immunological systems in senescent mice[J].Acta Pharmacol Sin,2001,22(12):1078-1083.
[9]刘涛,张荣超,黄悦.跑台运动对D-半乳糖致阿尔茨海默病模型大鼠干预的实验研究[J].陕西医学杂志,2014,43(2):149-151.
[10]党胜利,白石.中等负荷跑台训练对D-半乳糖致衰老大鼠基底前脑-皮质自由基代谢的影响[J].西安体育学院学报,2009,25(3):347-350.
[11]Hiramastsu M,Sasaki M,Kameyama T.Effects of dynorphin A(1-13)on carbon monoxide-induced delayed amnesia in mice studied in a step-down type passive avoidance task[J].Eur J Pharmacol,1995,282(1-3):185-191.
[12]赵燕燕,刘新霞,陈春生,等.运动训练对老年痴呆小鼠的改善作用[J].中国老年学杂志,2010,30(7):931-934.
[13]Kalesnykas G,Puolivali J,Sirvio J,et al.Cholinergic neurons in the basal forebrain of aged mice[J].Brain Res,2004,1002:148-156.
[14]何胜,玉莹,陈菊芳,等.D-半乳糖加亚硝酸钠建立痴呆症小鼠模型和中药治疗的效果[J].中国老年医学杂志,2014,34(1):422-424.
[15]官斌,李玉梅,马俊,等.不同工艺制品的补肾中药对痴呆小鼠乙酰胆碱酯酶活性和M-受体功能的影响[J].标记免疫分析与临床,1997,4(3):152-156.
[2]高谦,吴宗耀,姚志彬,等.短期和长期训练对小鼠脑局灶缺血后大脑皮质胆碱能纤维密度的影响[J].中国临床康复,2006,10(34):61-63.
[3]高谦,吴宗耀,姚志彬,等.运动训练对小鼠脑局灶缺血后大脑皮质胆碱能纤维密度的作用[J].中国临床康复,2003,7(16):2274-2275.
[4]陈运才,姚志彬,顾耀铭,等.运动对小鼠海马结构和大脑皮质胆碱能纤维的影响[J].神经解剖学杂志,1994,10(1):11-16.
[5]Tanka K,Dequadrosjr A C,Santos R F,et al.Benefits of physical execise on executive function in older people with Parkinson’s disease[J].Brain Cognition,2009,69(2):435-441.
[6]van Praag H,Shubert T,Zhao C,et al.Exercise enhances learning and hippocampal neurogenesis in agedmice[J].Neurosci,2005,25(38):8680-8685.
[7]Uysal N,Tuqyan K,Kayatekin B,et al.The effect of regular aerobic exercise in adolescent period on hippocampal neuron density,apoptosis and spatial memory[J].Neurosci Letters,2005,383:241-245
[8]Shang Y Z,gong M Y,Zhou X X,et al.Improving effects of SSF on memory deficits and pathological changes of neural and immunological systems in senescent mice[J].Acta Pharmacol Sin,2001,22(12):1078-1083.
[9]刘涛,张荣超,黄悦.跑台运动对D-半乳糖致阿尔茨海默病模型大鼠干预的实验研究[J].陕西医学杂志,2014,43(2):149-151.
[10]党胜利,白石.中等负荷跑台训练对D-半乳糖致衰老大鼠基底前脑-皮质自由基代谢的影响[J].西安体育学院学报,2009,25(3):347-350.
[11]Hiramastsu M,Sasaki M,Kameyama T.Effects of dynorphin A(1-13)on carbon monoxide-induced delayed amnesia in mice studied in a step-down type passive avoidance task[J].Eur J Pharmacol,1995,282(1-3):185-191.
[12]赵燕燕,刘新霞,陈春生,等.运动训练对老年痴呆小鼠的改善作用[J].中国老年学杂志,2010,30(7):931-934.
[13]Kalesnykas G,Puolivali J,Sirvio J,et al.Cholinergic neurons in the basal forebrain of aged mice[J].Brain Res,2004,1002:148-156.
[14]何胜,玉莹,陈菊芳,等.D-半乳糖加亚硝酸钠建立痴呆症小鼠模型和中药治疗的效果[J].中国老年医学杂志,2014,34(1):422-424.
[15]官斌,李玉梅,马俊,等.不同工艺制品的补肾中药对痴呆小鼠乙酰胆碱酯酶活性和M-受体功能的影响[J].标记免疫分析与临床,1997,4(3):152-156.