巨噬细胞移动抑制因子基因多态性与子宫内膜异位症的相关性研究
|
摘要
目的探讨巨噬细胞移动抑制因子(MIF)基因多态性与子宫内膜异位症遗传易感性的关系。方法选取卵巢型子宫内膜异位症行腹腔镜手术的患者150例作为病例组,同期健康体检者或因其他原因进行腹腔镜手术的患者150例作为对照组,采用聚合酶链式反应-限制性片段长度多态性分析技术通过外周静脉血检测MIF-rs4822446 A/G、rs4822443 G/A、rs2012133 G/C基因多态性,并分析基因多态性与子宫内膜异位症的关系。结果两组受试者MIF-rs4822446 A/G位点的等位基因和基因型频率比较差异均无统计学意义(均P>0.05)。两组受试者MIF-rs4822443 G/A位点的等位基因和基因频率比较差异均有统计学意义(均P<0.05),其中携带A等位基因的妇女患子宫内膜异位症的风险较携带G等位基因的妇女高(OR=1.145,95%CI:1.053～1.246,P<0.05)。两组受试者MIF-rs2012133 G/C位点的等位基因和基因频率比较差异均有统计学意义(均P<0.05),其中携带C等位基因的妇女患子宫内膜异位症的风险较携带G等位基因的妇女高(OR=1.208,95%CI:1.038～1.407,P<0.05)。结论 MIF-rs4822443 G/A、rs2012133 G/C位点多态性与与子宫内膜异位症遗传易感性有关。
Objective To investigate the relationship between the polymorphisms of macrophage migration inhibitory factor(MIF)gene and genetic susceptibility of endometriosis(EMS). Methods 150 patients with pathologically diagnosed EMS(EMS group) and 150 healthy women or non-EMS female patients(control group) were recruited in the study. The genotypes of MIF-rs4822446 A/G, rs4822443 G/A,rs2012133 G/C were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis, and the relationship between gene polymorphisms and EMS was analyzed. Results There was significant difference in allele distributions of MIF-rs4822443 G/A site between two groups(both P<0.05); compared with the G allele, the A allele significantly increased the risk of developing EMS with an odds ratio of 1.145(95%CI: 1.053-1.246, P<0.05). There was also significant difference in allele distributions of the MIF-rs2012133 G/C site between two groups(both P<0.05); compared with the G allele, the C allele significantly increased the risk of developing EMS with an odds ratio of 1.208(95%CI: 1.038-1.407, P<0.05). However, there was no significant difference in allele distribution of MIF-rs4822446 A/G site between two groups(P >0.05). Conclusion The results suggest that MIF-rs4822443 G/A, rs2012133 G/C polymorphisms are associated with the susceptibility to EMS.
Objective To investigate the relationship between the polymorphisms of macrophage migration inhibitory factor(MIF)gene and genetic susceptibility of endometriosis(EMS). Methods 150 patients with pathologically diagnosed EMS(EMS group) and 150 healthy women or non-EMS female patients(control group) were recruited in the study. The genotypes of MIF-rs4822446 A/G, rs4822443 G/A,rs2012133 G/C were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis, and the relationship between gene polymorphisms and EMS was analyzed. Results There was significant difference in allele distributions of MIF-rs4822443 G/A site between two groups(both P<0.05); compared with the G allele, the A allele significantly increased the risk of developing EMS with an odds ratio of 1.145(95%CI: 1.053-1.246, P<0.05). There was also significant difference in allele distributions of the MIF-rs2012133 G/C site between two groups(both P<0.05); compared with the G allele, the C allele significantly increased the risk of developing EMS with an odds ratio of 1.208(95%CI: 1.038-1.407, P<0.05). However, there was no significant difference in allele distribution of MIF-rs4822446 A/G site between two groups(P >0.05). Conclusion The results suggest that MIF-rs4822443 G/A, rs2012133 G/C polymorphisms are associated with the susceptibility to EMS.
引文
[1]王倩男,李沛霖,王建玲,等.子宫内膜异位症恶变的临床研究进展[J].浙江医学, 2017, 39(7):585-588. DOI:10.12056/j.issn.1006-2785.2017.39.7.2016-2034.
[2]俞丹华.巨噬细胞移动抑制因子、血管生成素-2、血管内皮生长因子在子宫内膜异位症中的意义[J].临床和实验医学杂志, 2015, 14(2):101-103. DOI:10.3969/j.issn.1671-4695.2015.02.008.
[3]Mahdian S, Aflatoonian R, Yazdi RS, et al. Macrophage migration inhibitory factor as a potential biomarker of endometriosis[J]. Fertility and Sterility, 2015, 103(1):153-159. DOI:10.1016/j.fertnstert.2014.09.031.
[4]Rakhila H, Girard K, Leboeuf M, et al. Macrophage migration inhibitory factor is involved in ectopic endometrial tissue growth and peritoneal-endometrial tissue interaction in vivo:a plausible link to endometriosis development[J]. PLo S One, 2014, 9(10):e110434. DOI:10.1371/journal.pone.0110434.
[5]Sampson JA. Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation[J].Am J Pathol, 1927, 3(2):93-110.
[6]Strathy JH, Molgaard CA, Coulam CB, et al. Endometriosis and infertility:a laparoscopic study of endometriosis among fertile and infertile women[J]. Fertil Steril, 1982, 38(6):667-672. DOI:10.1016/S0015-0282(16)46691-4.
[7]Steele RW, Dmowski WP, Marmer DJ. Immunologic aspects of human endometriosis[J]. Am J Reprod Immunol, 1984, 6(1):33-36.DOI:10.1111/j.1600-0897.1984.tb00106.x.
[8]Larson DF, Horak K. Macrophage migration inhibitory factor:controller of systemic inflammation[J]. Crit Care, 2006, 10(2):138.DOI:10.1186/cc4899.
[9]李建,胡玉红.血清MIF在子宫内膜异位症中临床应用价值[J].牡丹江医学院学报, 2016, 37(3):16-18. DOI:10.13799/j.cnki.mdjyxyxb.2016.03.006.
[10]王春平,秦成路,周芸,等. MIF基因启动子区-794CATT微卫星多态性与子宫内膜异位症相关性研究[J].中国妇幼保健, 2011,26(7):1086-1089.
[11]于雅,袁清连,王春平,等. MIF基因多态性与子宫内膜异位症的相关性[J].中国热带医学, 2013,13(5):625-628. DOI:10.13604/j.cnki.46-1064/r.2013.05.040.
[12]石瑾秋,张怡.巨噬细胞移动抑制因子-173位点单核苷酸多态性与子宫内膜异位症关系的研究[J].实用妇产科杂志, 2010, 26(9):668-671.
[2]俞丹华.巨噬细胞移动抑制因子、血管生成素-2、血管内皮生长因子在子宫内膜异位症中的意义[J].临床和实验医学杂志, 2015, 14(2):101-103. DOI:10.3969/j.issn.1671-4695.2015.02.008.
[3]Mahdian S, Aflatoonian R, Yazdi RS, et al. Macrophage migration inhibitory factor as a potential biomarker of endometriosis[J]. Fertility and Sterility, 2015, 103(1):153-159. DOI:10.1016/j.fertnstert.2014.09.031.
[4]Rakhila H, Girard K, Leboeuf M, et al. Macrophage migration inhibitory factor is involved in ectopic endometrial tissue growth and peritoneal-endometrial tissue interaction in vivo:a plausible link to endometriosis development[J]. PLo S One, 2014, 9(10):e110434. DOI:10.1371/journal.pone.0110434.
[5]Sampson JA. Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation[J].Am J Pathol, 1927, 3(2):93-110.
[6]Strathy JH, Molgaard CA, Coulam CB, et al. Endometriosis and infertility:a laparoscopic study of endometriosis among fertile and infertile women[J]. Fertil Steril, 1982, 38(6):667-672. DOI:10.1016/S0015-0282(16)46691-4.
[7]Steele RW, Dmowski WP, Marmer DJ. Immunologic aspects of human endometriosis[J]. Am J Reprod Immunol, 1984, 6(1):33-36.DOI:10.1111/j.1600-0897.1984.tb00106.x.
[8]Larson DF, Horak K. Macrophage migration inhibitory factor:controller of systemic inflammation[J]. Crit Care, 2006, 10(2):138.DOI:10.1186/cc4899.
[9]李建,胡玉红.血清MIF在子宫内膜异位症中临床应用价值[J].牡丹江医学院学报, 2016, 37(3):16-18. DOI:10.13799/j.cnki.mdjyxyxb.2016.03.006.
[10]王春平,秦成路,周芸,等. MIF基因启动子区-794CATT微卫星多态性与子宫内膜异位症相关性研究[J].中国妇幼保健, 2011,26(7):1086-1089.
[11]于雅,袁清连,王春平,等. MIF基因多态性与子宫内膜异位症的相关性[J].中国热带医学, 2013,13(5):625-628. DOI:10.13604/j.cnki.46-1064/r.2013.05.040.
[12]石瑾秋,张怡.巨噬细胞移动抑制因子-173位点单核苷酸多态性与子宫内膜异位症关系的研究[J].实用妇产科杂志, 2010, 26(9):668-671.