摘要
目的探讨巨噬细胞移动抑制因子(MIF)基因多态性与子宫内膜异位症遗传易感性的关系。方法选取卵巢型子宫内膜异位症行腹腔镜手术的患者150例作为病例组,同期健康体检者或因其他原因进行腹腔镜手术的患者150例作为对照组,采用聚合酶链式反应-限制性片段长度多态性分析技术通过外周静脉血检测MIF-rs4822446 A/G、rs4822443 G/A、rs2012133 G/C基因多态性,并分析基因多态性与子宫内膜异位症的关系。结果两组受试者MIF-rs4822446 A/G位点的等位基因和基因型频率比较差异均无统计学意义(均P>0.05)。两组受试者MIF-rs4822443 G/A位点的等位基因和基因频率比较差异均有统计学意义(均P<0.05),其中携带A等位基因的妇女患子宫内膜异位症的风险较携带G等位基因的妇女高(OR=1.145,95%CI:1.053~1.246,P<0.05)。两组受试者MIF-rs2012133 G/C位点的等位基因和基因频率比较差异均有统计学意义(均P<0.05),其中携带C等位基因的妇女患子宫内膜异位症的风险较携带G等位基因的妇女高(OR=1.208,95%CI:1.038~1.407,P<0.05)。结论 MIF-rs4822443 G/A、rs2012133 G/C位点多态性与与子宫内膜异位症遗传易感性有关。
Objective To investigate the relationship between the polymorphisms of macrophage migration inhibitory factor(MIF)gene and genetic susceptibility of endometriosis(EMS). Methods 150 patients with pathologically diagnosed EMS(EMS group) and 150 healthy women or non-EMS female patients(control group) were recruited in the study. The genotypes of MIF-rs4822446 A/G, rs4822443 G/A,rs2012133 G/C were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis, and the relationship between gene polymorphisms and EMS was analyzed. Results There was significant difference in allele distributions of MIF-rs4822443 G/A site between two groups(both P<0.05); compared with the G allele, the A allele significantly increased the risk of developing EMS with an odds ratio of 1.145(95%CI: 1.053-1.246, P<0.05). There was also significant difference in allele distributions of the MIF-rs2012133 G/C site between two groups(both P<0.05); compared with the G allele, the C allele significantly increased the risk of developing EMS with an odds ratio of 1.208(95%CI: 1.038-1.407, P<0.05). However, there was no significant difference in allele distribution of MIF-rs4822446 A/G site between two groups(P >0.05). Conclusion The results suggest that MIF-rs4822443 G/A, rs2012133 G/C polymorphisms are associated with the susceptibility to EMS.
引文
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