鸡滑液囊支原体感染的诊断与防控
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摘要
旨在为鸡滑液囊支原体感染的检测、诊断和防控提供参考。通过查阅与鸡滑液囊支原体(MS)感染的检测、诊断和防控相关的文献资料发现:血清学检测方法操作简单、诊断快速,常作为MS感染的初步诊断方法,但其存在常出现非特异性交叉反应而影响诊断准确性的问题;分离培养MS病原的难度大、耗时长,不能快速做出诊断;分子生物学诊断方法快速、灵敏,有明显的优势,但技术条件要求较高。最终确诊需要多种诊断方法联合使用,可采用ELISA与PCR法检测,并结合临床症状和病理变化来判断鸡场MS的感染情况。通过采取净化种鸡群、加强饲养管理及生物安全管理、接种疫苗等措施可有效防控MS感染。
This review aimed to provide references for the detection, diagnosis, prevention and control of infections caused by Mycoplasma synoviae(MS) in Chicken. The previously published literatures demonstrated that the serological detection methods were commonly used as the preliminary diagnosis means for MS infection in chicken due to their simple and fast operation process. However, the non-specific cross reaction always affects the diagnostic accuracy. The traditional isolation and in vitro culture of MS had difficulty and always took a long time and thus fast diagnosis could not be made. The molecular biological diagnostic techniques had obvious advantages in detection speed and sensitivity, but the requirements on technical conditions were relatively higher. The confirmative diagnosis of MS infection in chicken should be made by the combined use of the above methods, for instance, the combined use of ELISA, PCR assay, clinical symptoms observation and pathological changes inspection. Purifying breeding chicken flocks, enhancing feeding and management and biological safety management, and vaccinating are proved to be effective in control of MS infection in chicken farms.
This review aimed to provide references for the detection, diagnosis, prevention and control of infections caused by Mycoplasma synoviae(MS) in Chicken. The previously published literatures demonstrated that the serological detection methods were commonly used as the preliminary diagnosis means for MS infection in chicken due to their simple and fast operation process. However, the non-specific cross reaction always affects the diagnostic accuracy. The traditional isolation and in vitro culture of MS had difficulty and always took a long time and thus fast diagnosis could not be made. The molecular biological diagnostic techniques had obvious advantages in detection speed and sensitivity, but the requirements on technical conditions were relatively higher. The confirmative diagnosis of MS infection in chicken should be made by the combined use of the above methods, for instance, the combined use of ELISA, PCR assay, clinical symptoms observation and pathological changes inspection. Purifying breeding chicken flocks, enhancing feeding and management and biological safety management, and vaccinating are proved to be effective in control of MS infection in chicken farms.
引文
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[10]刘轶秋,薛青红,张媛,等.恩诺沙星及其他抗菌药对鸡滑液囊支原体的体外联合抑菌试验[J].中国家禽,2013,35(22):13-16.
[11]郭龙宗,李玉燕,崔治中.哈伯德曾祖代鸡支原体净化状态的维持和检测[J].中国家禽,2018,40(5):65-69.
[2]雷元元,郭磊,陈信,等.鸡滑液囊支原体病研究进展[J].农业科学研究,2017,38(1):57-60.
[3]丁美娟,张小飞,尹秀凤,等.鸡滑液囊支原体的分离和鉴定研究[J].中国家禽,2011,33(24):27-29.
[4]孙洪志.蛋鸡滑液囊支原体病的临床特点、鉴别和防控措施[J].现代畜牧科技,2017(1):92.
[5]侯宏艳,惠文巧,张丹俊,等.绵羊肺炎支原体的培养和PCR鉴定[J].畜牧与饲料科学,2016,37(11):97-98.
[6]黎敏,管凤霞,刘玲,等.应用多重套式PCR检测鸡毒支原体和鸡滑液囊支原体[J].广东畜牧兽医科技,2010,35(4):34-36.
[7]张磊.鸡毒支原体JN株的分离鉴定及PCR检测方法的建立[D].泰安:山东农业大学,2010.
[8]刘婷.鸡滑液囊支原体JS1株的分离鉴定及禽支原体、大肠杆菌、沙门菌多重PCR检测方法的建立[D].泰安:山东农业大学,2016.
[9]李燕,郭龙宗,石海春.鸡滑液囊支原体病的诊断和防治措施[J].家禽科学,2017(11):27-29.
[10]刘轶秋,薛青红,张媛,等.恩诺沙星及其他抗菌药对鸡滑液囊支原体的体外联合抑菌试验[J].中国家禽,2013,35(22):13-16.
[11]郭龙宗,李玉燕,崔治中.哈伯德曾祖代鸡支原体净化状态的维持和检测[J].中国家禽,2018,40(5):65-69.