NaCl预处理诱导大豆幼苗的抗旱性
|
摘要
为探明盐预处理对大豆幼苗抗旱性的影响,用0.6%NaCl预处理大豆幼苗后,再用100 g/L的PEG-6000模拟干旱胁迫大豆幼苗,研究了NaCl预处理对耐盐品种‘早熟毛豆’和盐敏感品种‘1号毛豆’抗旱性的影响。研究结果表明,PEG胁迫抑制了大豆幼苗的生长和根毛发育,降低了保护酶活性,提高了活性氧的积累,其中,耐盐品种‘早熟毛豆’根毛长度、直径、主根长、株高和保护酶活性等的降低幅度均高于盐敏感品种‘1号毛豆’,活性氧增加幅度低于盐敏感品种‘1号毛豆’。NaCl预处理后两品种根系生长抑制情况均得到了缓解,保护酶活性得到了提高,活性氧积累量降低。
In order to explore the effect of salt pretreatment on drought resistance of soybean seedlings, the influence of NaCl pretreatment on drought resistance of salt-tolerant soybean seedling ‘early-maturing soybean'and salt-sensitive seedling ‘No.1 soybean'were studied by pretreating soybean seedlings with 0.6% NaCl, and then simulating the drought environment by 100 g/L of PEG-6000. The results showed that the growth and root hair development of soybean seedlings were inhibited by PEG stress, and the activity of protective enzymes was reduced while the accumulation of reactive oxygen was increased. Moreover, the reduction of root hair length,diameter, main root length, plant height and protective enzyme activities of the salt-tolerant variety‘earlymaturing soybean'were higher than those of salt-sensitive variety‘No.1 soybean', and its increase of reactive oxygen was lower compared with salt-sensitive variety ‘No.1 soybean'. The inhibition of root growth of two varieties were eased, the protective enzyme activity was improved, and the amount of accumulation of active oxygen was reduced after NaCl pretreatment.
In order to explore the effect of salt pretreatment on drought resistance of soybean seedlings, the influence of NaCl pretreatment on drought resistance of salt-tolerant soybean seedling ‘early-maturing soybean'and salt-sensitive seedling ‘No.1 soybean'were studied by pretreating soybean seedlings with 0.6% NaCl, and then simulating the drought environment by 100 g/L of PEG-6000. The results showed that the growth and root hair development of soybean seedlings were inhibited by PEG stress, and the activity of protective enzymes was reduced while the accumulation of reactive oxygen was increased. Moreover, the reduction of root hair length,diameter, main root length, plant height and protective enzyme activities of the salt-tolerant variety‘earlymaturing soybean'were higher than those of salt-sensitive variety‘No.1 soybean', and its increase of reactive oxygen was lower compared with salt-sensitive variety ‘No.1 soybean'. The inhibition of root growth of two varieties were eased, the protective enzyme activity was improved, and the amount of accumulation of active oxygen was reduced after NaCl pretreatment.
引文
Li Z.,Wang X.J.,Peng D.D.,and Peng Y.,2013,Effects of Na+on antioxidant defense and organic osmotic adjustment substances in white clover under water stress,Caoye Xuebao(Acta Prataculturae Sinica),23(5):175-183(李州,王晓娟,彭丹丹,彭燕,2013,Na+对水分胁迫下白三叶抗氧化防御和有机渗透调节物质的影响,草业学报,23(5):175-183)
Li H.S.,ed.,2000,Plant physiological and biochemical experimental principles and techniques,Higher Education Publishing,Beijing,China,pp.164-169,258-260(李合生,编著,2000,植物生理生化实验原理和技术,高等教育出版,中国,北京,pp.164-169,258-260)
Liu J.,Lv B.,and Xu L.L.,2000,Improvement of determination methods of hydrogen peroxide in plant leaves,Shengwu Huaxue Yu Shengwu Wuli Jinzhan(Progress in Biochemistry and Biophysics),27(5):548-551(刘俊,吕波,徐朗莱,2000,植物叶片中过氧化氢含量测定方法的改进,生物化学与生物物理进展,27(5):548-551)
Ma Q.,Lou J.Q.,and Wang S.M.,2010,Effects of Na+on photosynthetic characteristics of Zygophyllum xanthonylom seedlings under osmotic stress,Caoye Xuebao(Acta Prataculturae Sinica),19(3):198-203(马清,楼洁琼,王锁民,2010,Na+对渗透胁迫下霸王幼苗光合特性的影响,草业学报,19(3):198-203)
Ren F.,2012,Effects of pretreatment on antioxidant enzymes and endogenous hormone of rice under water stress,Thesis for M.S.,Shenyang Normal University,Supervisor:Li X.M.,pp.1-40(任菲,2012,预处理对水分胁迫下水稻幼苗抗氧化酶和内源激素的影响,硕士学位论文,沈阳师范大学,导师:李雪梅,pp.1-40)
Wang Z.J.,Wang J.,Jiao X.Y.,Chen Y.S.,Xu Z.D.,Ou Z.L.,Wu X.,and He Z.A.,2016,Effects of salt stress and exogenous calcium on seed germination of Rhus chinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(3):706-714(王占军,王静,焦小雨,陈延松,徐忠东,欧祖兰,吴席,何子昂,2016,盐胁迫及外源钙处理对盐肤木种子萌发的影响,基因组学与应用生物学,35(3):706-714)
Wang A.G.,and Luo G.H.,1990,Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants,Zhiwu Shenglixue Tongxun(Plant Physiology Communication),(6):55-57(王爱国,罗广华,1990,植物的超氧物自由基与羟胺反应的定量关系,植物生理学通讯,(6):55-57)
Wu H.J.,2007,The reaction mechanism of cross-adaptation to drought and chilling stresses in wheat seedlings,cold cross adaptation,Thesis for M.S.,Northwest A&F University,Supervisor:Hu J.J.,pp.1-34(武杭菊,2007,小麦幼苗对干旱,低温逆境交叉适应的反应机制,硕士学位论文,西北农林科技大学,导师:胡景江,pp.1-34)
Xu F.F.,and Ye L.M.,2015,Studies on the adaptive mechanism of rice seedlings under the cross stress of drought and high temperature,Zajiao Shuidao(Hybrid Rice),30(5):70-73(徐芬芬,叶利民,2015,水稻幼苗对干旱和高温交叉逆境的适应机制研究,杂交水稻,30(5):70-73)
Zhou R.L.,Zhao Y.H.,Zhao H.L.,and Zhao X.Y.,2012,Ecological and physiological analysis of effect of different types of sodium salt treatment on winter wheat resistance to drought,Zhongguo Shamo(China Desert),32(3):784-792(周瑞莲,赵彦宏,赵哈林,赵学勇,2012,钠盐对冬小麦抗旱性增效作用调控机理的生理生态学分析,中国沙漠,32(3):784-792)
Li H.S.,ed.,2000,Plant physiological and biochemical experimental principles and techniques,Higher Education Publishing,Beijing,China,pp.164-169,258-260(李合生,编著,2000,植物生理生化实验原理和技术,高等教育出版,中国,北京,pp.164-169,258-260)
Liu J.,Lv B.,and Xu L.L.,2000,Improvement of determination methods of hydrogen peroxide in plant leaves,Shengwu Huaxue Yu Shengwu Wuli Jinzhan(Progress in Biochemistry and Biophysics),27(5):548-551(刘俊,吕波,徐朗莱,2000,植物叶片中过氧化氢含量测定方法的改进,生物化学与生物物理进展,27(5):548-551)
Ma Q.,Lou J.Q.,and Wang S.M.,2010,Effects of Na+on photosynthetic characteristics of Zygophyllum xanthonylom seedlings under osmotic stress,Caoye Xuebao(Acta Prataculturae Sinica),19(3):198-203(马清,楼洁琼,王锁民,2010,Na+对渗透胁迫下霸王幼苗光合特性的影响,草业学报,19(3):198-203)
Ren F.,2012,Effects of pretreatment on antioxidant enzymes and endogenous hormone of rice under water stress,Thesis for M.S.,Shenyang Normal University,Supervisor:Li X.M.,pp.1-40(任菲,2012,预处理对水分胁迫下水稻幼苗抗氧化酶和内源激素的影响,硕士学位论文,沈阳师范大学,导师:李雪梅,pp.1-40)
Wang Z.J.,Wang J.,Jiao X.Y.,Chen Y.S.,Xu Z.D.,Ou Z.L.,Wu X.,and He Z.A.,2016,Effects of salt stress and exogenous calcium on seed germination of Rhus chinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(3):706-714(王占军,王静,焦小雨,陈延松,徐忠东,欧祖兰,吴席,何子昂,2016,盐胁迫及外源钙处理对盐肤木种子萌发的影响,基因组学与应用生物学,35(3):706-714)
Wang A.G.,and Luo G.H.,1990,Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants,Zhiwu Shenglixue Tongxun(Plant Physiology Communication),(6):55-57(王爱国,罗广华,1990,植物的超氧物自由基与羟胺反应的定量关系,植物生理学通讯,(6):55-57)
Wu H.J.,2007,The reaction mechanism of cross-adaptation to drought and chilling stresses in wheat seedlings,cold cross adaptation,Thesis for M.S.,Northwest A&F University,Supervisor:Hu J.J.,pp.1-34(武杭菊,2007,小麦幼苗对干旱,低温逆境交叉适应的反应机制,硕士学位论文,西北农林科技大学,导师:胡景江,pp.1-34)
Xu F.F.,and Ye L.M.,2015,Studies on the adaptive mechanism of rice seedlings under the cross stress of drought and high temperature,Zajiao Shuidao(Hybrid Rice),30(5):70-73(徐芬芬,叶利民,2015,水稻幼苗对干旱和高温交叉逆境的适应机制研究,杂交水稻,30(5):70-73)
Zhou R.L.,Zhao Y.H.,Zhao H.L.,and Zhao X.Y.,2012,Ecological and physiological analysis of effect of different types of sodium salt treatment on winter wheat resistance to drought,Zhongguo Shamo(China Desert),32(3):784-792(周瑞莲,赵彦宏,赵哈林,赵学勇,2012,钠盐对冬小麦抗旱性增效作用调控机理的生理生态学分析,中国沙漠,32(3):784-792)