Xpert MTB/RIF技术对结核分枝杆菌的诊断价值
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摘要
目的对Xpert MTB/RIF技术检测结核分枝杆菌(MTB)的结果进行分析并评估其价值。方法采集2017年10月～2018年10月本院就诊疑为结核分枝杆菌感染的患者共580例,利用Xpert MTB/RIF技术对其痰液(或体液)标本进行DNA检测,同时将标本进行固体培养及涂片抗酸染色并对检测结果进行比较。结果 Xpert MTB/RIF技术检测MTB的阳性率为18.97%(110/580),和固体培养法的阳性率18.62%(108/580)相比差异无统计学意义(P>0.05);和抗酸染色法的阳性率15.00%(87/580)相比则更为敏感,差异有统计学意义(P<0.05)。结论 Xpert MTB/RIF检测技术和固体培养法不相上下,且由于抗酸染色法,可以用于MTB的快速筛选。
Objective To analyze and evaluate the results of Xpert MTB/RIF detection of Mycobacterium tuberculosis(MTB).Methods A total of 580 patients with suspected Mycobacterium tuberculosis infection were enrolled in our hospital from October 2017 to October 2018. The sputum(or body fluid) specimens were tested for DNA by Xpert MTB/RIF technique. Solid culture and smear acid-fast staining and comparison of test results. Results The positive rate of MTB detected by Xpert MTB/RIF technique was 18.97%(110/580), and the positive rate of solid culture method was 18.62%(108/580),the difference was not statistically significant(P>0.05); The positive rate of staining was 15.00%(87/580), which was more sensitive,the difference was statistically significant(P<0.05). Conclusion Xpert MTB/RIF detection technology is comparable to solid culture method, and can be used for rapid screening of MTB due to acid-fast staining.
Objective To analyze and evaluate the results of Xpert MTB/RIF detection of Mycobacterium tuberculosis(MTB).Methods A total of 580 patients with suspected Mycobacterium tuberculosis infection were enrolled in our hospital from October 2017 to October 2018. The sputum(or body fluid) specimens were tested for DNA by Xpert MTB/RIF technique. Solid culture and smear acid-fast staining and comparison of test results. Results The positive rate of MTB detected by Xpert MTB/RIF technique was 18.97%(110/580), and the positive rate of solid culture method was 18.62%(108/580),the difference was not statistically significant(P>0.05); The positive rate of staining was 15.00%(87/580), which was more sensitive,the difference was statistically significant(P<0.05). Conclusion Xpert MTB/RIF detection technology is comparable to solid culture method, and can be used for rapid screening of MTB due to acid-fast staining.
引文
[1]赵雁林,姜广路.痰涂片镜检标准化操作及质量保证手册[M].北京:中国协和医科大学出版社,2009.
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[3]刘亚芹,杨振斌,冯冬霞,等.GeneXpert法检测结核分枝杆菌及其对利福平耐药性的研究[J].中华实验和临床感染病杂志(电子版),2015(4):524-527.
[4]李妍,张天华,鲜小平,等.Xpert Mtb/RIF检测技术诊断肺结核和肺外结核的价值[J].中国防痨杂志,2015,37(6):586-589.
[5]刘婷,温贵华,陈伊,等.XpertMTB/RIF在结核病诊断及利福平耐药中的应用[J].国际检验医学杂志,2016,37(10):1368-1370.
[6]周洪经,郭明日,冯爽,等.Xpert MTB/RIF在快速诊断肺结核及利福平耐药中的临床应用[J].国际检验医学杂志,2016,37(18):2568-2570.
[7]张爱梅,李锋,刘旭晖,等.结核分枝杆菌/利福平耐药实时荧光定量核酸扩增检测在肺外结核中的诊断价值[J].中华传染病杂志,2016,34(3):174-179.
[8]陈子芳,劳海黎,李秀华,等.全自动医用PCR分析系统在肺外结核诊断及利福平耐药检测中的应用价值[J].中华结核和呼吸杂志,2016,39(7):529-533.
[9]邹远妩,刘尚武,朱蕾,等.Xpert MTB/RIF检测结核分枝杆菌及其耐药性在结核病辅助诊断中的临床价值[J].临床肺科杂志,2015(3):568-570.
[10]马秀珍,卓玛.高海拔地区Xpert MTB/RIF检测结核分枝杆菌及其耐药性在结核病辅助诊断中的应用价值[J].广东微量元素科学,2016,23(9):34-36.
[2]赵雁林,王黎霞.分枝杆菌分离培养标准化操作程序及质量保证手册[M].北京:人民卫生出版社,2013.
[3]刘亚芹,杨振斌,冯冬霞,等.GeneXpert法检测结核分枝杆菌及其对利福平耐药性的研究[J].中华实验和临床感染病杂志(电子版),2015(4):524-527.
[4]李妍,张天华,鲜小平,等.Xpert Mtb/RIF检测技术诊断肺结核和肺外结核的价值[J].中国防痨杂志,2015,37(6):586-589.
[5]刘婷,温贵华,陈伊,等.XpertMTB/RIF在结核病诊断及利福平耐药中的应用[J].国际检验医学杂志,2016,37(10):1368-1370.
[6]周洪经,郭明日,冯爽,等.Xpert MTB/RIF在快速诊断肺结核及利福平耐药中的临床应用[J].国际检验医学杂志,2016,37(18):2568-2570.
[7]张爱梅,李锋,刘旭晖,等.结核分枝杆菌/利福平耐药实时荧光定量核酸扩增检测在肺外结核中的诊断价值[J].中华传染病杂志,2016,34(3):174-179.
[8]陈子芳,劳海黎,李秀华,等.全自动医用PCR分析系统在肺外结核诊断及利福平耐药检测中的应用价值[J].中华结核和呼吸杂志,2016,39(7):529-533.
[9]邹远妩,刘尚武,朱蕾,等.Xpert MTB/RIF检测结核分枝杆菌及其耐药性在结核病辅助诊断中的临床价值[J].临床肺科杂志,2015(3):568-570.
[10]马秀珍,卓玛.高海拔地区Xpert MTB/RIF检测结核分枝杆菌及其耐药性在结核病辅助诊断中的应用价值[J].广东微量元素科学,2016,23(9):34-36.