电针不同腧穴对急慢性结肠炎大鼠模型血清IL-6含量的影响
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摘要
目的:观察电针不同腧穴对急慢性结肠炎大鼠模型血清IL-6含量的影响。方法:急性实验取60只清洁级大鼠,随机分为空白组、模型组、大肠俞组、天枢组、足三里组和上巨虚组,每组10只,雌雄各半,选用6%冰乙酸溶液灌肠制备急性结肠炎大鼠模型,空白组灌肠同体积生理盐水。第3天治疗后,麻醉后腹主动脉采血,ELISA法检测血清IL-6含量。慢性实验大鼠分组及检测指标同急性实验,用免疫法加局部刺激制备慢性结肠炎大鼠模型。结果:急性结肠炎实验中,与空白组相比,模型组血清IL-6含量升高;与模型组比较,各腧穴组血清IL-6含量均降低,差异有统计学意义。慢性结肠炎实验中,与空白组相比,模型组血清IL-6含量升高;电针各腧穴组血清IL-6含量均较模型组降低,差异有统计学意义;治疗10 d后,天枢组IL-6含量下降较其他组明显。结论:电针各腧穴对急性与慢性结肠炎的治疗作用可能与下调血清IL-6含量有关,其中电针天枢组作用更显著。
Objective:To observe the effects of electric acupuncture at different acupoints on the content of serum IL-6 in rats with acute or chronic colitis. Methods:Acute experiment:60 clean rats were random Ly divided into blank group,model group,Dachangshu group,Tianshu group,Zusanli group and Shangjuxu group,and there were 5 males and 5 females in each group. Models were prepared by 6% ice acetic acid enema.3 days after treatment,blood was extracted from the abdominal aorta after anesthesia,Serum IL-6 was detected by ELISA. Chronic experiment:The rat models of chronic colitis were prepared by immunological method and local stimulation. The method of experiment was same as acute experiment. Results:Acute colitis experiment,compared with the blank group,serum IL-6 content was increased in model group.The serum IL-6 levels in acupoints groups were lower than that of model group. Chronic colitis experiment:compared with the blank group,content of serum IL-6 was increased in model;the content of serum IL-6 in acupoint groups was lower than that of model group;10 days after treatment,the content of serum IL-6 of Tianshu group was significantly lower than that of other groups. Conclusion:The method of electric acupuncture at different acupoints was effective to acute and chronic colitis.Its mechanism may be related to reduction of the serum IL-6 content,and the effect of the Tianshu group was more significant.
Objective:To observe the effects of electric acupuncture at different acupoints on the content of serum IL-6 in rats with acute or chronic colitis. Methods:Acute experiment:60 clean rats were random Ly divided into blank group,model group,Dachangshu group,Tianshu group,Zusanli group and Shangjuxu group,and there were 5 males and 5 females in each group. Models were prepared by 6% ice acetic acid enema.3 days after treatment,blood was extracted from the abdominal aorta after anesthesia,Serum IL-6 was detected by ELISA. Chronic experiment:The rat models of chronic colitis were prepared by immunological method and local stimulation. The method of experiment was same as acute experiment. Results:Acute colitis experiment,compared with the blank group,serum IL-6 content was increased in model group.The serum IL-6 levels in acupoints groups were lower than that of model group. Chronic colitis experiment:compared with the blank group,content of serum IL-6 was increased in model;the content of serum IL-6 in acupoint groups was lower than that of model group;10 days after treatment,the content of serum IL-6 of Tianshu group was significantly lower than that of other groups. Conclusion:The method of electric acupuncture at different acupoints was effective to acute and chronic colitis.Its mechanism may be related to reduction of the serum IL-6 content,and the effect of the Tianshu group was more significant.
引文
[1]祁超,闫丽萍,燕平,等.电针腧穴“肠病方”对UC模型大鼠IL-6及NF-κBp65的影响[J].山西中医学院学报,2013,14(4):2-4.
[2]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:425-430.
[4]徐叔云.药理实验方法学[M].北京:人民卫生出版社,1982:891.
[4]陈治水,张志清,聂志伟,等.溃疡性结肠炎动物模型与健脾灵复健作用的研究[J].中西医结合杂志,1990,10(8):488-490.
[5]刘万里,沈洪,单兆伟,等.肠安胶囊修复溃疡性结肠炎大鼠结肠损伤研究[J].天津中医学院学报,2003,22(4):7-9.
[6]陈楚淘,严杰,田浩梅,等.电针天枢配大肠俞、天枢配上巨虚对溃疡性结肠炎模型大鼠血清NO、MDA影响的比较研究[J].中医药导报,2008,14(1):1-3.
[7]葛维.艾灸天枢和足三里对UC大鼠结肠组织中MMP-1和TIMP-1表达的影响[D].沈阳:辽宁中医药大学,2014.
[8]双源,闫丽萍,燕平,等.电针腧穴“肠病方”对肠黏膜损伤大鼠MPO和IL-10表达影响[J].山西中医学院学报,2013,14(3):4-6.
[2]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:425-430.
[4]徐叔云.药理实验方法学[M].北京:人民卫生出版社,1982:891.
[4]陈治水,张志清,聂志伟,等.溃疡性结肠炎动物模型与健脾灵复健作用的研究[J].中西医结合杂志,1990,10(8):488-490.
[5]刘万里,沈洪,单兆伟,等.肠安胶囊修复溃疡性结肠炎大鼠结肠损伤研究[J].天津中医学院学报,2003,22(4):7-9.
[6]陈楚淘,严杰,田浩梅,等.电针天枢配大肠俞、天枢配上巨虚对溃疡性结肠炎模型大鼠血清NO、MDA影响的比较研究[J].中医药导报,2008,14(1):1-3.
[7]葛维.艾灸天枢和足三里对UC大鼠结肠组织中MMP-1和TIMP-1表达的影响[D].沈阳:辽宁中医药大学,2014.
[8]双源,闫丽萍,燕平,等.电针腧穴“肠病方”对肠黏膜损伤大鼠MPO和IL-10表达影响[J].山西中医学院学报,2013,14(3):4-6.