“中脘”“足三里”不同刺激参数组合对正常大鼠胃内压的影响
|
摘要
目的:观察"中脘""足三里"不同刺激参数组合对正常大鼠胃内压的影响,探索"中脘""足三里"的配伍运用规律。方法:按照进针顺序("中脘"先针/"足三里"先针)和电针刺激强度组合("中脘"1mA和"足三里"1mA、"中脘"1mA和"足三里"2mA、"中脘"1mA和"足三里"4mA)将SD大鼠随机分为6组,每组15只。在动物胃窦放置球囊,连接压力感受器和多导电生理记录系统并记录胃内压,应用2×3×4析因分析方法分析0～30s、30～60s、60～90s、90～120s4个时间段各组电针干预对胃内压的变化倍率值影响,观察电针"中脘""足三里"不同刺激参数组合在不同时间段对大鼠胃内压的影响。结果:在0～30s时间段内,"中脘"对胃内压的效应以抑制为主(P<0.05),"足三里"穴显示出兴奋的效应趋势(P>0.05)。与0～30s的效应相比,各组电针在30～120s的效应量降低(P<0.01)。在"中脘"1mA和"足三里"1mA刺激下,进针顺序可影响0～30s的效应,对30～120s的效应没有影响;在"中脘"1mA和"足三里"2mA刺激下,进针顺序可影响0～30s和90～120s的效应;在"中脘"1mA和"足三里"4mA刺激下,进针顺序可影响0～120s的效应;"中脘"先针的情况下,"足三里"刺激强度加大到4mA可明显拮抗"中脘"的效应。结论:"中脘"和"足三里"配伍应用可产生拮抗效应,这一效应受进针顺序、电针刺激强度和针刺时间的影响。
Objective To observe the effect of electroacupuncture(EA)of"Zhongwan"(CV12)and"Zusanli"(ST36)in different combinations of stimulating parameters on intragastric pressure(IGP)in normal rats so as to explore their best combinations for promoting gastrointestinal mobility.Methods A total of 90 male SD rats were randomly divided into 6 EA groups:CV12-1 mA+ST36-1 mA,CV12-1 mA+ST36-2 mA,CV12-1 mA+ST36-4 mA,ST36-1 mA+CV12-1 mA,ST36-2 mA+CV12-1 mA,and ST36-4 mA+CV12-1 mA which the first acupoint was stimulated first,followed by the second in each group(n=15 rats/group).Before(1 min),and 0—30 s,30—60 s,60—90 s,and 90—120 sduring EA stimulation of the left ST36 or CV12 first or later,the IGP was measured via an inserted intragastric balloon,a connected pressure transducer and an amplifier.Changes of the IGP were analyzed using 2×3×4 factorial design.Results 1)During 0—30 s,EA-CV12 showed an obvious inhibitory effect on IGP(P<0.05)while EA-ST36 showed a mild exciting effect(P>0.05).2)Compared with the IGP level of 0—30 s,the IGP levels of 30—120 swere significantly decreased in all the groups(P<0.01).3)In the CV12-1 mA/ST 36-1 mA groups,only the IGP level of 0—30 swas affected by the EA-stimulating order(P<0.05).In the CV12-1 mA/ST36-2 mA groups,both the IGP levels during 0—30 sand 90—120 swere obviously affected by EA-stimulating sequence.In the CV12-1 mA/ST36-4 mA groups,the IGP level during 0—120 swas affected by EA-stimulating order.4)Only in the condition of EA-CV12 stimulating first and EAST36 second and at 4 mA,the reduction effect of IGP of EA-CV12 was antagonized.There are marked interaction effects between the EA strength and acupoint EA stimulating sequence,and between the time course and acupoint EA stimulating sequence(P<0.01),but no significant interaction effects were found between the time course and stimulating strength,and among the EA stimulating strength,time course and acupoint EA stimulating sequence(P>0.05).Conclusion Simultaneous EA stimulation of ST36 and CV12 has an antagonistic effect on IGP in normal rats,which is affected by the stimulating sequence,stimulating strength and time course.
Objective To observe the effect of electroacupuncture(EA)of"Zhongwan"(CV12)and"Zusanli"(ST36)in different combinations of stimulating parameters on intragastric pressure(IGP)in normal rats so as to explore their best combinations for promoting gastrointestinal mobility.Methods A total of 90 male SD rats were randomly divided into 6 EA groups:CV12-1 mA+ST36-1 mA,CV12-1 mA+ST36-2 mA,CV12-1 mA+ST36-4 mA,ST36-1 mA+CV12-1 mA,ST36-2 mA+CV12-1 mA,and ST36-4 mA+CV12-1 mA which the first acupoint was stimulated first,followed by the second in each group(n=15 rats/group).Before(1 min),and 0—30 s,30—60 s,60—90 s,and 90—120 sduring EA stimulation of the left ST36 or CV12 first or later,the IGP was measured via an inserted intragastric balloon,a connected pressure transducer and an amplifier.Changes of the IGP were analyzed using 2×3×4 factorial design.Results 1)During 0—30 s,EA-CV12 showed an obvious inhibitory effect on IGP(P<0.05)while EA-ST36 showed a mild exciting effect(P>0.05).2)Compared with the IGP level of 0—30 s,the IGP levels of 30—120 swere significantly decreased in all the groups(P<0.01).3)In the CV12-1 mA/ST 36-1 mA groups,only the IGP level of 0—30 swas affected by the EA-stimulating order(P<0.05).In the CV12-1 mA/ST36-2 mA groups,both the IGP levels during 0—30 sand 90—120 swere obviously affected by EA-stimulating sequence.In the CV12-1 mA/ST36-4 mA groups,the IGP level during 0—120 swas affected by EA-stimulating order.4)Only in the condition of EA-CV12 stimulating first and EAST36 second and at 4 mA,the reduction effect of IGP of EA-CV12 was antagonized.There are marked interaction effects between the EA strength and acupoint EA stimulating sequence,and between the time course and acupoint EA stimulating sequence(P<0.01),but no significant interaction effects were found between the time course and stimulating strength,and among the EA stimulating strength,time course and acupoint EA stimulating sequence(P>0.05).Conclusion Simultaneous EA stimulation of ST36 and CV12 has an antagonistic effect on IGP in normal rats,which is affected by the stimulating sequence,stimulating strength and time course.
引文
[1]韩国伟.选用足三里、中脘、内关为“胃病基本方”文献简述[J].针灸临床杂志,2003,19(6):1-2.
[2]徐小茹,刘畅,韩明娟,等.针灸治疗胃溃疡取穴及配伍规律分析[J].中华针灸电子杂志,2014,3(6):32-36
[3]王灵.电针足三里、中脘对急性胃黏膜损伤大鼠NO、GAS、EGF的影响[J].针灸临床杂志,2010,26(7):59-61.
[4]彭楚湘,王灵,周国平,等.足三里配不同穴改善急性胃黏膜损伤作用的对比研究[J].中国针灸,2007,27(1):44-47.
[5]冀来喜,闫丽萍,王海军,等.电针保护大鼠急性胃黏膜损伤基本腧穴配伍“胃病方”的筛选[J].针刺研究,2008,33(5):296-300.
[6]钟峰,曾芳,郑晖,等.腧穴配伍拮抗作用的研究现状[J].中国针灸,2011,31(12):1093-1096.
[7]杨馥铭,郭义.试论腧穴配伍中的相须、相使与相恶[J].中国针灸,2015,35(10):1021-1024.
[8]刘文全,王健,郝志友.针刺对功能性消化不良胃肠动力影响的临床研究[J].中国针灸,2001,21(5):267-269.
[9]赵红,张轶.针刺治疗糖尿病性胃轻瘫34例临床观察[J].中国针灸,2004,24(3):167-168.
[10]刘坤.孤束核在针刺调节大鼠血压及胃肠感觉和运动中的作用[D].北京:中国中医科学院,2012.
[11]王述菊.针刺不同穴位对胃运动异常大鼠双向调节效应研究[D].武汉:湖北中医学院,2008.
[12]曾晓霞,马铁明.论针刺取穴顺序[J].吉林中医药,2013,33(9):939-941.
[13]洪俪凤,李学武.论“针序”[J].北京中医药大学学报(中医临床版),2003,10(1):52-53.
[14]马良宵,朱江.留针时间及下针顺序与疗效相关性的研究概述[J].中国针灸,2005,25(9):667-669.
[15]金侣位,朱江.灵龟八法“开”“闭”时辰取穴与“主”“客”顺序取穴针刺对晚孕大鼠子宫收缩活动的影响[J].针刺研究,2008,33(5):316-320.
[16]李丽,王祖红,易荣,等.灵龟八法择时取穴治疗腹泻型肠易激综合征临床观察[J].上海针灸杂志,2017,36(10):1181-1185.
[17]陈滢如,马良宵,朱江.传统针刺助产处方(合谷-三阴交穴)优化研究的回顾与展望[J].成都中医药大学学报,2009,32(3):4-7.
[18]马良宵,朱江,张露芬.“合谷-三阴交”穴不同的电针和留针时间组合对大鼠怀孕后期子宫收缩的影响[J].针刺研究,2006,31(4):223-227.
[19]徐鸿燕,杨芳,朱江,等.电针合谷、三阴交对药物流产近期副反应的影响[J].中国针灸,2007,27(2):103-105.
[20]王文升,金宏柱,王耀帅,等.不同电针强度与药物剂量刺激效应量的相对关系——针药效应差异性的实验研究[J].针灸临床杂志,2012,28(5):61-65.
[21]辛娟娟,宿杨帅,杨兆坤,等.不同强度电针、热灸样刺激对香草酸瞬时受体亚型1基因敲除小鼠痛阈的影响[J].针刺研究,2012,37(6):431-439.
[22]周杰芳,靳瑞.不同电针强度对高血压实证病人血压的影响[J].针刺研究,2004,29(4):286-288.
[23] SU Y S,HE W,WANG C,et al.“Intensity-response”effects of electroacupuncture on gastric motility and its underlying peripheral neural mechanism[J].Evid Based Complement Alternat Med,2013,2013:535742.
[2]徐小茹,刘畅,韩明娟,等.针灸治疗胃溃疡取穴及配伍规律分析[J].中华针灸电子杂志,2014,3(6):32-36
[3]王灵.电针足三里、中脘对急性胃黏膜损伤大鼠NO、GAS、EGF的影响[J].针灸临床杂志,2010,26(7):59-61.
[4]彭楚湘,王灵,周国平,等.足三里配不同穴改善急性胃黏膜损伤作用的对比研究[J].中国针灸,2007,27(1):44-47.
[5]冀来喜,闫丽萍,王海军,等.电针保护大鼠急性胃黏膜损伤基本腧穴配伍“胃病方”的筛选[J].针刺研究,2008,33(5):296-300.
[6]钟峰,曾芳,郑晖,等.腧穴配伍拮抗作用的研究现状[J].中国针灸,2011,31(12):1093-1096.
[7]杨馥铭,郭义.试论腧穴配伍中的相须、相使与相恶[J].中国针灸,2015,35(10):1021-1024.
[8]刘文全,王健,郝志友.针刺对功能性消化不良胃肠动力影响的临床研究[J].中国针灸,2001,21(5):267-269.
[9]赵红,张轶.针刺治疗糖尿病性胃轻瘫34例临床观察[J].中国针灸,2004,24(3):167-168.
[10]刘坤.孤束核在针刺调节大鼠血压及胃肠感觉和运动中的作用[D].北京:中国中医科学院,2012.
[11]王述菊.针刺不同穴位对胃运动异常大鼠双向调节效应研究[D].武汉:湖北中医学院,2008.
[12]曾晓霞,马铁明.论针刺取穴顺序[J].吉林中医药,2013,33(9):939-941.
[13]洪俪凤,李学武.论“针序”[J].北京中医药大学学报(中医临床版),2003,10(1):52-53.
[14]马良宵,朱江.留针时间及下针顺序与疗效相关性的研究概述[J].中国针灸,2005,25(9):667-669.
[15]金侣位,朱江.灵龟八法“开”“闭”时辰取穴与“主”“客”顺序取穴针刺对晚孕大鼠子宫收缩活动的影响[J].针刺研究,2008,33(5):316-320.
[16]李丽,王祖红,易荣,等.灵龟八法择时取穴治疗腹泻型肠易激综合征临床观察[J].上海针灸杂志,2017,36(10):1181-1185.
[17]陈滢如,马良宵,朱江.传统针刺助产处方(合谷-三阴交穴)优化研究的回顾与展望[J].成都中医药大学学报,2009,32(3):4-7.
[18]马良宵,朱江,张露芬.“合谷-三阴交”穴不同的电针和留针时间组合对大鼠怀孕后期子宫收缩的影响[J].针刺研究,2006,31(4):223-227.
[19]徐鸿燕,杨芳,朱江,等.电针合谷、三阴交对药物流产近期副反应的影响[J].中国针灸,2007,27(2):103-105.
[20]王文升,金宏柱,王耀帅,等.不同电针强度与药物剂量刺激效应量的相对关系——针药效应差异性的实验研究[J].针灸临床杂志,2012,28(5):61-65.
[21]辛娟娟,宿杨帅,杨兆坤,等.不同强度电针、热灸样刺激对香草酸瞬时受体亚型1基因敲除小鼠痛阈的影响[J].针刺研究,2012,37(6):431-439.
[22]周杰芳,靳瑞.不同电针强度对高血压实证病人血压的影响[J].针刺研究,2004,29(4):286-288.
[23] SU Y S,HE W,WANG C,et al.“Intensity-response”effects of electroacupuncture on gastric motility and its underlying peripheral neural mechanism[J].Evid Based Complement Alternat Med,2013,2013:535742.