酶解法催化鹅油制备甘油二酯
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摘要
为提高鹅油的附加值,开发天然食品添加剂,本试验以肥肝鹅腹脂精炼后的鹅油为脂肪酸供体制备甘油二酯。以鹅油混合脂肪酸、甘油为原料,甘油二酯的生成率为指标,底物比(脂肪酸∶甘油)、加酶量、反应时间及反应温度为因素,采用响应面法对鹅油甘油二酯的制备工艺进行了优化。结果表明:反应温度为46℃,反应时间为9.2 h,底物比为1.2,加酶量为3.03%时,甘油二酯的生成率为92.57%。
The experiment has been conducted to improve the added value of goose oil and develop natural food additives. The goose oil produced by foie gras goose has been taken as donator of fatty acid to prepare diglyceride.Goose oil,fatty acids and glycerol were mixed and utilized as raw materials. Generation rate of diglyceride was indicator,substrate ratio( fatty acid∶ glycerol),amount of enzyme,reaction time and temperature were factors. The preparation process of goose oil diglyceride was optimized by surface methodology. The results showed that the best condition was the reaction temperature 46 ℃,reaction time 9. 2 h,substrate ratio 1. 12 and enzyme dosage 3. 03%. The generation rate of goose oil diglyceride finally reached to 92. 57%.
The experiment has been conducted to improve the added value of goose oil and develop natural food additives. The goose oil produced by foie gras goose has been taken as donator of fatty acid to prepare diglyceride.Goose oil,fatty acids and glycerol were mixed and utilized as raw materials. Generation rate of diglyceride was indicator,substrate ratio( fatty acid∶ glycerol),amount of enzyme,reaction time and temperature were factors. The preparation process of goose oil diglyceride was optimized by surface methodology. The results showed that the best condition was the reaction temperature 46 ℃,reaction time 9. 2 h,substrate ratio 1. 12 and enzyme dosage 3. 03%. The generation rate of goose oil diglyceride finally reached to 92. 57%.
引文
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[12]李相,刘云,杨江科,等.基于响应面设计脂肪酶Novo435催化合成甘油二酯的工艺优化[J].生物加工过程,2009,7(5):13-18
[13]徐扬,王卫飞,陈华勇,等.偏甘油酯脂肪酶Lipase G50催化酯化法制备甘油二酯[J].中国油脂,2012,37(2):46-50
[14]任运宏,孙博,刘晶,等.超临界体系酶法催化甘油解制备甘油二酯的研究[J].食品工业科技,2011,32(10):337-340
[15]邱寿宽,毕艳,杨天奎,等.Lipozyme RM IM酶促豆油甘油解制备甘二酯的研究[J].郑州工程学院学报,2004,25(1):33-36
[16]钟南京,董玲燕,李琳,等.酶法酯化脂肪酸与甘油合成1,3-甘油二酯[J].河南工业大学学报,2010,31(2):59-61
[17]郑平玉,王卫飞,王永华,等.高纯度甘油二酯的酶法合成及性质研究[J].中国油脂,2013,38(3):43-46
[18]刘小如,张超,胡蒋宁,等.酶催化茶油合成甘油二酯工艺及脂肪酸变化[J].食品科学,2013,34(2):36-41
[19]Phuong L V,Rae K P,Yun J L.Two-step production of oil enriched in conjugated linoleic acids and diacylglycerol[J].Journal of American Oil Chemists Society,2007,84:123-128.
[2]FDA.GRAS Notice No.GRN 000056[EB/OL].http://www.Fda.gov/Food/Food Ingredients Packaging/Generally Recognized as Safe GRAS/GRASListings/ucm153749.htm,2000
[3]Tsuneo Y,Sung T K,Katsuyoshi K,et al.High Yield diacylglycerol formation by solid-phase enzymatic glycerolysis of hydrogenated beef tallow[J].Journal of American Oil Chemists'Society,1994,71(3):339-342
[4]Morita O,Soni M G.Safety assessment of diacylglycerol oil as an edible oil:a review of the published literature[J].Food and Chemical Toxicology,2009,47:9-21
[5]Hiroyuki T,Tomonori N,Hiroyuki W.Energy value and digestibility of dietary oil containing mainly 1,3-diacylglycerol are similar to those of triacylglycerol[J].Lipids,2001,36(8):379-382
[6]Meng X H,Zou D Y,Shi Z P,et al.Dietary diacylglycerol prevents high-fatdiet-induced lipid accumulation in pat liverand abdominal adipose tissue[J].Lipids,2004,39(1):37-41
[7]刘艳丰,黄惠华.酶催化亚麻籽油甘油解制备富含α-亚麻酸的甘油二酯的研究[J].食品工业科技,2012,33(7):216-219
[8]Roberta L.K,Alexsandra V,Manuela B,et al.Improvement of mono and diacylglycerol production via enzymatic glycerolysis in tert-butanol system[J].European Journal of Lipid Science and Technology,2010,112(8):921-927
[9]李鹤,刘云,徐莉,等.甘油二酯的酶法合成工艺研究[J].应用化工,2011,40(1):8-12
[10]李磊,黄建花,金青哲,等.酶法催化制备富含甘油二酯米糠油的研究[J].中国油脂,2011,36(7):36-40
[11]陈晓慧,王雪,刘晶,等.超临界体系酶催化制备甘油二酯及其纯化[J].食品科学,2012,33(2):43-47
[12]李相,刘云,杨江科,等.基于响应面设计脂肪酶Novo435催化合成甘油二酯的工艺优化[J].生物加工过程,2009,7(5):13-18
[13]徐扬,王卫飞,陈华勇,等.偏甘油酯脂肪酶Lipase G50催化酯化法制备甘油二酯[J].中国油脂,2012,37(2):46-50
[14]任运宏,孙博,刘晶,等.超临界体系酶法催化甘油解制备甘油二酯的研究[J].食品工业科技,2011,32(10):337-340
[15]邱寿宽,毕艳,杨天奎,等.Lipozyme RM IM酶促豆油甘油解制备甘二酯的研究[J].郑州工程学院学报,2004,25(1):33-36
[16]钟南京,董玲燕,李琳,等.酶法酯化脂肪酸与甘油合成1,3-甘油二酯[J].河南工业大学学报,2010,31(2):59-61
[17]郑平玉,王卫飞,王永华,等.高纯度甘油二酯的酶法合成及性质研究[J].中国油脂,2013,38(3):43-46
[18]刘小如,张超,胡蒋宁,等.酶催化茶油合成甘油二酯工艺及脂肪酸变化[J].食品科学,2013,34(2):36-41
[19]Phuong L V,Rae K P,Yun J L.Two-step production of oil enriched in conjugated linoleic acids and diacylglycerol[J].Journal of American Oil Chemists Society,2007,84:123-128.