乳腺癌细胞中缺氧诱导因子-1α与Survivin表达的相关性及其临床价值探讨
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摘要
目的探讨乳腺癌细胞中缺氧诱导因子-1α(HIF-1α)与生存素(Survivin)表达的相关性及其临床价值。方法选取2017年5月～2018年8月我院及深圳市人民医院收治的乳腺导管内增生病变及乳腺癌患者130例,对所有患者的HIF-1α、Survivin含量进行检测。分析HIF-1α、Survivin在乳腺导管内增生病变及乳腺癌不同组织中的表达情况,HIF-1α、Survivin在乳腺导管内增生病变及乳腺癌中的表达情况与临床病理之间的关系、HIF-1α与Survivin表达的相关性。结果 HIF-1α、Survivin在乳腺浸润性癌中的阳性表达率高于其他组织(P<0.05);HIF-1α、Survivin在乳腺导管内增生病变及乳腺癌中的表达情况与患者的年龄、肿瘤大小无明显关系(P>0.05),肿瘤发生远处转移、淋巴结转移、组织学分级高其表达阳性率较高(P<0.05);在乳腺导管内增生病变及乳腺癌中,HIF-1α与Survivin的表达呈正相关(P<0.05)。结论乳腺导管内增生病变及乳腺癌细胞中HIF-1α与Survivin表达呈正相关,HIF-1α、Survivin在乳腺导管内增生病变及乳腺癌中的高表达可能与乳腺导管内增生病变及乳腺癌发生远处转移密切相关,可以通过HIF-1α、Survivin在乳腺导管内增生病变及乳腺癌中的表达情况来判断患者的病情进展,进而有利于为临床治疗提供一定的依据,有较高的临床价值。
Objective To study the clinical value and correlation between expression of survivin and hypoxiainducible factor-1α in breast cancer cell. Methods 130 cases of intraductal hyperplasia of breast and breast cancer patients in our hospital and Shenzhen People's Hospital from May 2017 to Aug 2018 were selected to test the content of HIF-1α and survivin. Analyzed the expression of HIF-1α and survivin in different tissues, the relation between expression of HIF-1α and survivin in breast cancer cells and clinical pathology, and the correlation of HIF-1α and survivin expression. Results Positive expression rate of HIF-1α and survivin in invasive breast cancer cells was higher than in other tissues(P<0.05); the expression of HIF-1α and survivin in breast cancer was irrelative with the age and tumor size of patients(P>0.05). Breast cancer with distant metastasis, lymph node metastasis, histological grading has high positive expression rate of HIF-1α and survivin(P<0.05). HIF-1α was positively correlated with survivin expression in breast cancer(P<0.05). Conclusion High expression of HIF-1α and survivin in breast cancer was possible to be closely correlated with distant metastasis of breast cancer. During the study of correlation, HIF-1α in breast cancer cells is positively correlated with survivin expression, which can be used to assess the severity of breast cancer. That is helpful in clinical therapy and has high clinical value.
Objective To study the clinical value and correlation between expression of survivin and hypoxiainducible factor-1α in breast cancer cell. Methods 130 cases of intraductal hyperplasia of breast and breast cancer patients in our hospital and Shenzhen People's Hospital from May 2017 to Aug 2018 were selected to test the content of HIF-1α and survivin. Analyzed the expression of HIF-1α and survivin in different tissues, the relation between expression of HIF-1α and survivin in breast cancer cells and clinical pathology, and the correlation of HIF-1α and survivin expression. Results Positive expression rate of HIF-1α and survivin in invasive breast cancer cells was higher than in other tissues(P<0.05); the expression of HIF-1α and survivin in breast cancer was irrelative with the age and tumor size of patients(P>0.05). Breast cancer with distant metastasis, lymph node metastasis, histological grading has high positive expression rate of HIF-1α and survivin(P<0.05). HIF-1α was positively correlated with survivin expression in breast cancer(P<0.05). Conclusion High expression of HIF-1α and survivin in breast cancer was possible to be closely correlated with distant metastasis of breast cancer. During the study of correlation, HIF-1α in breast cancer cells is positively correlated with survivin expression, which can be used to assess the severity of breast cancer. That is helpful in clinical therapy and has high clinical value.
引文
1任丽,柳雅慧,夏文超,等.乳腺癌细胞缺氧微环境中癌胚抗原的表达及其对预后的判断意义[J].中华检验医学杂志,2016,39(9):710-714
2李娜,贺国洋,李永真,等.沉默缺氧诱导因子-2α对乳腺癌细胞化疗敏感性的影响[J].临床与实验病理学杂志,2017,56(5):33-36
3 Liang G,Liu Z,Tan L,et al.HIF1α-associated circDENND4CPromotes Proliferation of Breast Cancer Cells in Hypoxic Environment[J].Anticancer Research,2017,37(8):4337-4343
4牛司华,朱庆莉,姜玉新,等.乳腺癌超声光散射断层成像与缺氧诱导因子-1α的相关性[J].中国医学科学院学报,2016,38(3):341-345
5李伯和,袁磊.地高辛对缺氧诱导的乳腺癌细胞上皮间质转化和侵袭的影响[J].中国病理生理杂志,2016,32(5):852-856
6王强修.乳腺导管内增生性病变诊断与治疗[M].北京:人民军医出版社,2009:76
7 Li M,Xiao D,Zhang J,et al.Expression of LPA2 is associated with poor prognosis in human breast cancer and regulates HIF-1αexpression and breast cancer cell growth[J].Oncology Reports,2016,36(6):3479-3487
8曹珍,高玉雪,徐金媛,等.远华蟾毒精对小鼠乳腺癌细胞侵袭的抑制作用及机制[J].山东医药,2017,16(37):24-26
9李鸿丽.三七皂苷Ft1抑制乳腺癌细胞增殖、迁移及促进凋亡的机制研究[J].药学学报,2016,51(7):1091-1097
10 Hristova M,Rocha-Ferreira E,Fontana X,et al.Inhibition of Signal Transducer and Activator of Transcription 3(STAT3)reduces neonatal hypoxic-ischaemic brain damage[J].Journal of Neurochemistry,2016,136(5):981-994
11侯培锋,陈强.α-酮戊二酸二甲酯抑制乳腺癌细胞增殖的机制研究[J].中国药理学通报,2016,32(5):738-739
12刘兆华,卢思英,张振涛.Survivin和Smac在乳腺癌中的表达及临床意义[J].重庆医学,2017,36(14):100-101
13 Zhao Q,Tan BB,Li Y,et al.Enhancement of Drug Sensitivity by Knockdown of HIF-1αin Gastric Carcinoma Cells[J].Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,2016,23(3):129-136
14张钊华.Survivin蛋白表达水平与乳腺癌患者预后生存期关联的Meta分析[J].中国老年学杂志,2016,36(4):864-867
15黄雅娟,杜峰,魏玲丽.miR-335通过靶向生存素对乳腺癌细胞增殖的调控作用[J].重庆医学,2016,45(27):3753-3756
16孙宇,邹燕鹏,邓素华,等.不同分子分型乳腺癌原发灶内CD4+、CD8+T淋巴细胞的表达差异研究[J].中国临床研究,2016,29(2):153-156
17 Dabral S,Tian X,Kojonazarov B,et al.Notch1 signalling regulates endothelial proliferation and apoptosis in pulmonary arterial hypertension[J].European Respiratory Journal,2016,48(4):1137-1149
18贺艳.Survivin及P-糖蛋白在乳腺癌组织中的表达及意义[J].现代肿瘤医学,2017,25(5):724-728
2李娜,贺国洋,李永真,等.沉默缺氧诱导因子-2α对乳腺癌细胞化疗敏感性的影响[J].临床与实验病理学杂志,2017,56(5):33-36
3 Liang G,Liu Z,Tan L,et al.HIF1α-associated circDENND4CPromotes Proliferation of Breast Cancer Cells in Hypoxic Environment[J].Anticancer Research,2017,37(8):4337-4343
4牛司华,朱庆莉,姜玉新,等.乳腺癌超声光散射断层成像与缺氧诱导因子-1α的相关性[J].中国医学科学院学报,2016,38(3):341-345
5李伯和,袁磊.地高辛对缺氧诱导的乳腺癌细胞上皮间质转化和侵袭的影响[J].中国病理生理杂志,2016,32(5):852-856
6王强修.乳腺导管内增生性病变诊断与治疗[M].北京:人民军医出版社,2009:76
7 Li M,Xiao D,Zhang J,et al.Expression of LPA2 is associated with poor prognosis in human breast cancer and regulates HIF-1αexpression and breast cancer cell growth[J].Oncology Reports,2016,36(6):3479-3487
8曹珍,高玉雪,徐金媛,等.远华蟾毒精对小鼠乳腺癌细胞侵袭的抑制作用及机制[J].山东医药,2017,16(37):24-26
9李鸿丽.三七皂苷Ft1抑制乳腺癌细胞增殖、迁移及促进凋亡的机制研究[J].药学学报,2016,51(7):1091-1097
10 Hristova M,Rocha-Ferreira E,Fontana X,et al.Inhibition of Signal Transducer and Activator of Transcription 3(STAT3)reduces neonatal hypoxic-ischaemic brain damage[J].Journal of Neurochemistry,2016,136(5):981-994
11侯培锋,陈强.α-酮戊二酸二甲酯抑制乳腺癌细胞增殖的机制研究[J].中国药理学通报,2016,32(5):738-739
12刘兆华,卢思英,张振涛.Survivin和Smac在乳腺癌中的表达及临床意义[J].重庆医学,2017,36(14):100-101
13 Zhao Q,Tan BB,Li Y,et al.Enhancement of Drug Sensitivity by Knockdown of HIF-1αin Gastric Carcinoma Cells[J].Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,2016,23(3):129-136
14张钊华.Survivin蛋白表达水平与乳腺癌患者预后生存期关联的Meta分析[J].中国老年学杂志,2016,36(4):864-867
15黄雅娟,杜峰,魏玲丽.miR-335通过靶向生存素对乳腺癌细胞增殖的调控作用[J].重庆医学,2016,45(27):3753-3756
16孙宇,邹燕鹏,邓素华,等.不同分子分型乳腺癌原发灶内CD4+、CD8+T淋巴细胞的表达差异研究[J].中国临床研究,2016,29(2):153-156
17 Dabral S,Tian X,Kojonazarov B,et al.Notch1 signalling regulates endothelial proliferation and apoptosis in pulmonary arterial hypertension[J].European Respiratory Journal,2016,48(4):1137-1149
18贺艳.Survivin及P-糖蛋白在乳腺癌组织中的表达及意义[J].现代肿瘤医学,2017,25(5):724-728