丹瓜方对转基因糖尿病小鼠心脏损伤的作用
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摘要
探讨丹瓜方对转基因糖尿病小鼠心脏组织的影响。32只ApoE-/-小鼠数字随机等分为模型组、丹瓜方组、联合用药组(丹瓜方+吡格列酮)、吡格列酮组。喂以普通饲料,各组分别给药12周。小鼠处死,取心脏、匀浆,免疫组化测心脏血清反应因子(SRF)含量;解剖冠状动脉,光镜观察比较。结果:与模型组比较,联合用药组与丹瓜方组SRF阳性率降低有显著性差异(P<0.01);形态学观察,丹瓜方组与联合用药组冠状动脉内膜大部分无增厚。结果表明:丹瓜方对糖尿病小鼠心脏具有保护作用。
To observe the effect of Dan Gua-Fang on the heart of genetically induced diabetic mice.Thirty-two ApoE-/-diabetic mice were randomly divided into the model group,Dan-gua prescription group,the combined treatment group(Dan-gua prescription + pioglitazone) and pioglitazone group.Each group was given medicine respectively,and fed with normal diet for 12 weeks.The heart homogenates production was used to measure related indicators.The expression of SRF in heart homogenates was detected by immunohistochemical.The Coronary was observed under light microscopy.Compared with the model group,there were significant difference of SRF positive rate between combination group and Dan-gua prescription group(P< 0.01).The morphology of Dan-gua prescription group and the combination group was significantly improved:most of the tunica intima of the coronary artery cannot be found thickening.Compared with the control group,Pioglitazone group's effect is weaker,but better than that of the untreated group.Dan Gua-Fang has protective effect on the heart of diabetic mice.
To observe the effect of Dan Gua-Fang on the heart of genetically induced diabetic mice.Thirty-two ApoE-/-diabetic mice were randomly divided into the model group,Dan-gua prescription group,the combined treatment group(Dan-gua prescription + pioglitazone) and pioglitazone group.Each group was given medicine respectively,and fed with normal diet for 12 weeks.The heart homogenates production was used to measure related indicators.The expression of SRF in heart homogenates was detected by immunohistochemical.The Coronary was observed under light microscopy.Compared with the model group,there were significant difference of SRF positive rate between combination group and Dan-gua prescription group(P< 0.01).The morphology of Dan-gua prescription group and the combination group was significantly improved:most of the tunica intima of the coronary artery cannot be found thickening.Compared with the control group,Pioglitazone group's effect is weaker,but better than that of the untreated group.Dan Gua-Fang has protective effect on the heart of diabetic mice.
引文
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[2]衡先培,陈可冀,洪振丰,等.高糖培养ECV304内ROS含量变化及丹瓜方影响的研究[J].光明中医,2008,23(5):551-555.
[3]Heng Xianpei,Chen Keji,Hong Zhengfeng,et a1.Glucose endothelial cytotoxicity and protection by Dan Gua-Fang,a Chinese herb prescription in huVEC in hyperglycemia medium[J].J Diabetes Complications,2009,23(5):297-303.
[4]衡先培,褚克丹,林青,等.丹瓜方对血糖控制不良2型糖尿病患者TNF-α的干预研究[J].福建中医学院学报,2009,19(2):9-12,21.
[5]韩雅玲,于海波,闫承慧,等.血清反应因子参与RhoA诱导的人血管内皮细胞肌动蛋白骨架重构[J].生理学报,2005,57(3):295-302.
[6]吴晓霞,智光,吴加金,等.反式作用因子中血清反应因子变化与心力衰竭关系的研究[J].中华心血管病杂志,2001,29(1):51-53.
[7]王陆颖.同型半胱氨酸血症所致大鼠仔鼠先天性心脏病与SRF基因表达的相关性研究[D].长沙:中南大学附属湘雅二医院,2009:12,45.
[8]王雪.创新中药F-苷肽防治糖尿病药效学及机理的初步研究[J].解放军药学学报,2006,22(2):81-84.
[9]Ciccocioppo R,Angeletti S,Panocka I,et a1.Nociceptin/orphanin FQ and drugs of abuse[J].Peptides,2000,21(7):1071-1080.
[10]Jin W,Goldfine A B,Boes T,et al.Increased SRF transcriptional activity in human and mouse skeletal muscle is a signature of insulin resistance[J].J Clin Invest,2011,121(3):918-929.