Nrf2对香烟烟雾提取物诱导的人支气管上皮细胞中IKKs表达的影响
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摘要
目的:探讨香烟烟雾提取物(cigarette smoke extract,CSE)刺激后人支气管上皮细胞中核因子E2相关因子2(nuclear factor E2-related factor 2,Nrf2)的表达与IκB激酶家族(I kappa B kinases,IKKs)的关系。方法:培养人支气管上皮细胞,分为正常对照(control)组、CSE组、15-脱氧-前列腺素J2(15-deoxy-prostaglandin J2,15dPGJ2)预处理后CSE刺激(15d-PGJ2+CSE)组和Nrf2 siRNA转染后CSE刺激(Nrf2 siRNA+CSE)组。首先通过CSE刺激人支气管上皮细胞,再通过15d-PGJ2预处理和Nrf2 siRNA转染的方法来增加和降低Nrf2的水平,并用RT-PCR法及Western blot法观察各组细胞Nrf2与IKKs的表达。结果:支气管上皮细胞在CSE的刺激下,Nrf2和IKKα/β的mRNA表达水平均明显升高,蛋白表达水平亦明显增高(P<0.05)。在支气管上皮细胞中,15d-PGJ2预处理可增加Nrf2的表达,而Nrf2 siRNA可成功抑制Nrf2的表达。Nrf2表达增加伴有IKKα/β表达明显降低;而Nrf2表达被抑制后,IKKα/β表达明显增加(P<0.05)。这些变化在转录水平和翻译水平是一致的。结论:Nrf2能抑制CSE诱导的人支气管上皮细胞中IKKs的表达。
AIM: To explore the expression of nuclear factor E2-related factor 2( Nrf2) in human bronchial epithelial cells after the stimulation of cigarette smoke extract( CSE) and the relationship with IκB kinases( IKKs). METHODS: Cultured bronchial epithelial cells were divided into normal control group,CSE group,pretreatment with 15-deoxyprostaglandin J2( 15 d-PGJ2) and stimulation with CSE( 15 d-PGJ2+ CSE) group,and pretreatment with Nrf2 siRNA and stimulation with CSE( Nrf2 siRNA + CSE) group. CSE was used to stimulate human bronchial epithelial cells,and then15 d-PGJ2 pretreatment and Nrf2 siRNA were used to increase and decrease Nrf2 level,respectively. The expression of Nrf2 and IKKs at mRNA and protein levels was determined by RT-PCR and Western blot. RESULTS: CSE stimulation significantly increased the expression of Nrf2 and IKKs in human bronchial epithelial cells( P < 0. 05). Compared with CSE intervention alone,15 d-PGJ2 pretreatment and CSE stimulation significantly increased Nrf2 expression. Nrf2 siRNA successfully inhibited the expression of Nrf2. Compared with CSE intervention alone,intervention with Nrf2 siRNA and CSE stimulation significantly reduced Nrf2 expression and significantly increased the IKKα/β expression( P < 0. 05). The changes at transcriptional level were consistent with those at translational level. CONCLUSION: Nrf2 inhibits the expression of IKKs in normal human bronchial epithelia cells induced by CSE.
AIM: To explore the expression of nuclear factor E2-related factor 2( Nrf2) in human bronchial epithelial cells after the stimulation of cigarette smoke extract( CSE) and the relationship with IκB kinases( IKKs). METHODS: Cultured bronchial epithelial cells were divided into normal control group,CSE group,pretreatment with 15-deoxyprostaglandin J2( 15 d-PGJ2) and stimulation with CSE( 15 d-PGJ2+ CSE) group,and pretreatment with Nrf2 siRNA and stimulation with CSE( Nrf2 siRNA + CSE) group. CSE was used to stimulate human bronchial epithelial cells,and then15 d-PGJ2 pretreatment and Nrf2 siRNA were used to increase and decrease Nrf2 level,respectively. The expression of Nrf2 and IKKs at mRNA and protein levels was determined by RT-PCR and Western blot. RESULTS: CSE stimulation significantly increased the expression of Nrf2 and IKKs in human bronchial epithelial cells( P < 0. 05). Compared with CSE intervention alone,15 d-PGJ2 pretreatment and CSE stimulation significantly increased Nrf2 expression. Nrf2 siRNA successfully inhibited the expression of Nrf2. Compared with CSE intervention alone,intervention with Nrf2 siRNA and CSE stimulation significantly reduced Nrf2 expression and significantly increased the IKKα/β expression( P < 0. 05). The changes at transcriptional level were consistent with those at translational level. CONCLUSION: Nrf2 inhibits the expression of IKKs in normal human bronchial epithelia cells induced by CSE.
引文
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[10]Ciucci A,Gianferretti P,Piva R,et al.Induction of apoptosis in estrogen receptor-negative breast cancer cells by natural and synthetic cyclopentenones:role of the IκB kinase/nuclear factor-κB pathway[J].Mol Pharmacol,2006,70(5):1812-1821.
[2]李秀英,罗百灵,陈红梅.核因子相关因子-2在慢性阻塞性肺疾病大鼠模型中的作用及其与I-κB激酶的关系[J].中华结核和呼吸杂志,2009,32(12):935-939.
[3]Li XY,Luo BL,Wang LJ,et al.15-Deoxy-prostaglandin J2 anti-inflammation in a rat model of chronic obstructive pulmonary disease and human bronchial epithelial cells via Nrf2 activation[J].Genetics Mol Res,2015,14(4):14037-14042.
[4]江刚,张卫东.香烟烟雾提取物通过蛋白激酶C-核因子相关因子2调节大鼠气道上皮细胞血红素加氧酶1表达[J].中国病理生理杂志,2014,30(8):1483-1488.
[5]Hoffmann A,Levchenko A,Scott ML,et al.The IκBNF-κB signaling module:temporal control and selective gene activation[J].Science,2002,298(5596):1241-1245.
[6]Ziegelbauei K,Gantner F,Lukacs NW,et al.A selective novel low-molecular-weight inhibitor of IκB kinase-β(IKK-β)prevents pulmonary inflammation and shows broad anti-flammatory activity[J].Br J Pharmacol,2005,145(2):178-192.
[7]Kim JH,Choi YK,Lee KS,et al.Functional dissection of Nrf2-dependent phase II genes in vascular inflammation and endotoxic injury using Keap1 siRNA[J].Free Radic Biol Med,2012,53(3):629-640.
[8]Ltoh K,Mochizki M,Lshii Y,et al.Transcription factor Nrf2 regulates inflammation by mediating the effect of 15-deoxy-Δ12,14-prostaglandin J2[J].Mol Cell Biol,2004,24(1):36-45.
[9]Mochizuki M,Ishii Y,Itoh K,et al.Role of 15-deoxy-Δ12,14prostaglandin J2and Nrf2 pathways in protection against acute lung injury[J].Am J Respir Crit Care Med,2005,171(11):1260-1266.
[10]Ciucci A,Gianferretti P,Piva R,et al.Induction of apoptosis in estrogen receptor-negative breast cancer cells by natural and synthetic cyclopentenones:role of the IκB kinase/nuclear factor-κB pathway[J].Mol Pharmacol,2006,70(5):1812-1821.