番茄红素抑制香烟烟雾提取物刺激巨噬细胞所致的炎症反应
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摘要
【目的】探讨番茄红素对香烟烟雾提取物(CSE)所诱导的人单核细胞株(THP-1)巨噬细胞炎症因子生成的影响及其作用的分子机制。【方法】用100μg/L佛波酯(PMA)诱导THP-1细胞48 h,使其分化为巨噬细胞后,分为空白组、CSE组和CSE+不同浓度番茄红素组(0.5、1和2μmol/L)。采用ELISA法检测THP-1巨噬细胞培养基中白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量,荧光探针DCFH-DA测定细胞内活性氧(ROS)生成水平,Western-blotting检测核p65的蛋白表达量。【结果】CSE诱导THP-1巨噬细胞后,IL-6和TNF-α合成释放水平升高,番茄红素可降低CSE诱导引起IL-6和TNF-α生成水平升高。CSE刺激后细胞内ROS生成及核p65蛋白表达增加,核因子-κB(NF-κB)活化,番茄红素可拮抗以上作用,阻止NF-κB活化。【结论】番茄红素能够有效抑制CSE诱导THP-1巨噬细胞炎症反应,该作用主要通过阻断CSE诱导细胞内ROS生成进而抑制NF-κB依赖的炎症因子IL-6和TNF-α分泌发挥的,这可能是番茄红素抑制吸烟导致的炎症反应的作用机制之一。
【Objective】To investigate the effects of lycopene on the production of proinflammatory cytokines in macro-phages of human monocyte line(THP-1)induced by cigarette smoke extract(CSE)and its possible molecular mecha-nisms.【Methods】100 μg/L phorbol ester(PMA)was used to induce THP-1 cells for 48 hours,after the differentiation ofTHP-1 cells,which became macrophage,and cells were divided into control group,CSE group and CSE combined withdifferent concentration(0.5,1,2 μmol/L)of lycopene. The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were assayed by ELISA. The level of reactive oxygen species(ROS)was detected by the methods of DCFH-DA.The level of nuclear factor-κB(NF-κB)P65 protein was analyzed by Western blotting.【Results】The levels of IL-6 andTNF-α were increased in the THP-1 macrophages after stimulated by CSE,lycopene could reduce the elevated levels ofIL-6 and TNF-α. The production of ROS and the expression of nuclear P65 significantly increased after stimulated by CSEand NF-κB was activated. Lycopene could reverse the above changes and prevent the activation of NF-κB.【Conclusion】Lycopene could inhibit CSE-induced inflammatory response in THP-1 macrophages through reducing CSE-stimulatedROS production and then inhibiting the NF-κB activation and the related proinflammatory cytokines production,which isone of the mechanisms responsible for preventing smoking induced-inflammation by lycopene.
【Objective】To investigate the effects of lycopene on the production of proinflammatory cytokines in macro-phages of human monocyte line(THP-1)induced by cigarette smoke extract(CSE)and its possible molecular mecha-nisms.【Methods】100 μg/L phorbol ester(PMA)was used to induce THP-1 cells for 48 hours,after the differentiation ofTHP-1 cells,which became macrophage,and cells were divided into control group,CSE group and CSE combined withdifferent concentration(0.5,1,2 μmol/L)of lycopene. The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were assayed by ELISA. The level of reactive oxygen species(ROS)was detected by the methods of DCFH-DA.The level of nuclear factor-κB(NF-κB)P65 protein was analyzed by Western blotting.【Results】The levels of IL-6 andTNF-α were increased in the THP-1 macrophages after stimulated by CSE,lycopene could reduce the elevated levels ofIL-6 and TNF-α. The production of ROS and the expression of nuclear P65 significantly increased after stimulated by CSEand NF-κB was activated. Lycopene could reverse the above changes and prevent the activation of NF-κB.【Conclusion】Lycopene could inhibit CSE-induced inflammatory response in THP-1 macrophages through reducing CSE-stimulatedROS production and then inhibiting the NF-κB activation and the related proinflammatory cytokines production,which isone of the mechanisms responsible for preventing smoking induced-inflammation by lycopene.
引文
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[13]Zhang Q,Lenardo MJ,Baltimore D. 30 Years of NF-κB:a blossoming of relevance to human pathobiology[J]. Cell,2017,168(1-2):37-57.
[14]Nishikawa M. Cigarette smoke-induced acute airway impairment[J]. Nihon Ko Ga Za,2000,38(5):347-353.
[15]Niemann B,Rohrbach S,Miller MR et al. Oxidative stress and cardiovascular risk:obesity,diabetes,smoking,and pollution:Part 3 of a 3-Part Series[J]. J Am Coll Cardiol,2017,70(2):230-251.
[16]Forrester SJ,Kikuchi DS,Hernandes MS,et al.Reactive oxygen species in metabolic and inflammatory signaling[J]. Circ Res,2018,122(6):877-902.
[17]Rao AV,Shen H. Effect of low dose lycopene intake on lycopene bioavailability and oxidative stress[J]. Nutr Res,2002,22(10):1125-1131.
[2]Zou J,Feng D. Lycopene reduces cholesterol absorption through the downregulation of NiemannPick C1-like 1 in Caco-2 cells[J]. Mol Nutr Food Res,2015,59(11):2225-2230.
[3]Costa-Rodrigues J,Pinho O,Monteiro PRR. Can lycopene be considered an effective protection against cardiovascular disease[J]? Food Chem,2018,15(245):1148-1153.
[4]Sesso HD,Liu S,Gaziano JM,et al. Dietary lycopene,tomato-based food products and cardiovascular disease in women[J]. J Nutr,2003,133(7):2336-2341.
[5]Zou J,Feng D,Ling W H,et al. Lycopene suppresses proinflammatory response in lipopolysaccharide-stimulated macrophages by inhibiting ROS-induced trafficking of TLR4 to lipid raft-like domains[J]. J Nutr Biochem,2013,24(6):1117-1122.
[6]冯丹,凌文华.番茄红素对脂多糖诱导巨噬细胞炎症反应的作用及其分子机制[J].中山大学学报(医学科学版),2011,32(4):421-425.Feng D,Ling WH. Effects of lycopene on lipopolysaccharide-induced inflammatory response in macrophages and its possible molecular mechanism[J].J Sun Yat-sen Univ(Med Sci),2011,32(4):421-425.
[7]Vayssier-Taussat M,Camilli T,Aron Y,et al. Effects of tobacco smoke and benzo[a]pyrene on human endothelial cell and monocyte stress responses[J]. Am J Physiol Heart Circ Physiol,2001,280(3):H1293-H1300.
[8]Feng D,Liu T,Wang H,et al. Mediation role of C-reactive protein on the association between smoking quantity and type 2 diabetes in current Chinese smokers[J]. J Diabetes Res,2014,18(23):171538-171541.
[9]Feng D,Liu T,Su DF,et al. The association between smoking quantity and hypertension mediated by inflammation in Chinese current smokers[J]. J Hypertens,2013,31(9):1798-1805.
[10]孙咏梅,戴树桂,袭著革.香烟烟雾成分分析及其对DNA生物氧化能力研究[J].环境与健康杂志,2001,18(4):203-207.Sun YM,Dai SG,Xi ZG. Analysis of components in tobacco smoke and study of its biological oxidative ability on DNA[J]. J Environ Health,2001,18(4):203-207.
[11]程攀,何庆,张晓宇,等.载气吹扫尼古丁长期吸入对大鼠心血管功能?氧化应激和炎症水平影响[J].安徽医科大学学报,2018,53(6):834-839.Cheng P,He Q,Zhang XY,et al. Effects of longterm inhalation of nicotine by gas-purge on cardiovascular function,oxidative stress and inflammation in rats[J]. Acta Uni Med Anhui,2018,53(6):834-839.
[12]Chiba T,Inoko H,Kimura M,et al. Role of nuclear IκBs in inflammation regulation[J]. Biomol Concepts,2013,4(2):187-196.
[13]Zhang Q,Lenardo MJ,Baltimore D. 30 Years of NF-κB:a blossoming of relevance to human pathobiology[J]. Cell,2017,168(1-2):37-57.
[14]Nishikawa M. Cigarette smoke-induced acute airway impairment[J]. Nihon Ko Ga Za,2000,38(5):347-353.
[15]Niemann B,Rohrbach S,Miller MR et al. Oxidative stress and cardiovascular risk:obesity,diabetes,smoking,and pollution:Part 3 of a 3-Part Series[J]. J Am Coll Cardiol,2017,70(2):230-251.
[16]Forrester SJ,Kikuchi DS,Hernandes MS,et al.Reactive oxygen species in metabolic and inflammatory signaling[J]. Circ Res,2018,122(6):877-902.
[17]Rao AV,Shen H. Effect of low dose lycopene intake on lycopene bioavailability and oxidative stress[J]. Nutr Res,2002,22(10):1125-1131.