肝癌中骨形态发生蛋白7表达及其与生存相关性分析
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摘要
目的研究肝癌组织中骨形态发生蛋白7(BMP-7)表达及其与患者预后关系。方法免疫组织化学及实时逆转录聚合酶链反应(real-time RT-PCR)方法分析肝癌组织、癌旁组织及非癌肝脏组织中BMP-7表达,选取2010年1月—2013年12月江苏省连云港市第一人民医院85例肝癌患者的癌组织及20例癌旁组织标本,以肝血管瘤及肝脏外伤患者14例为良性对照。研究BMP-7与肝癌患者临床病理特征之间关系及其不同表达患者术后生存情况。结果 BMP-7在肝癌组织、癌旁组织及非癌肝脏组织中表达的阳性率比较,差异有统计学意义(P<0.05),BMP-7的表达与患者肿瘤大小、肿瘤组织分化程度相关,其阳性表达的肝癌患者预后较阴性表达者差。结论 BMP-7与肝癌的发生、发展相关,可以作为预测患者预后情况的重要指标。
Objective To study the expression of BMP-7 in hepatocellular carcinoma(HCC) and its relationship with clinical prognosis. Methods A total of 85 patients with HCC admitted into our hospital from January 2010 to December 2013 were enrolled in this study. Twerty cases of pura-carcinoma tissnes and 14 cases of hepatic hemangioma or hepatic injury tissues were also collectd. Expression of BMP-7 in HCC tissues(n = 85), adjacent tissues(n = 20), and non-cancerous liver tissues(n = 14) was analyzed by immunohistochemistry and RT-PCR. Relationship between BMP-7 and clinicopathological features was investigated. Results The positive expression rate of BMP-7 in hepatocellular carcinoma, adjacent tissues and non-cancerous liver tissue was significantly different. The expression of BMP-7 was closely correlated to the tumor size and differentiation degree of tumor tissue. Patients with positive expression of BMP-7 experienced poor outcome. Conclusion BMP-7 may play an important role in the carcinogenesis and progression of HCC, which may be an important prognostic indicator.
Objective To study the expression of BMP-7 in hepatocellular carcinoma(HCC) and its relationship with clinical prognosis. Methods A total of 85 patients with HCC admitted into our hospital from January 2010 to December 2013 were enrolled in this study. Twerty cases of pura-carcinoma tissnes and 14 cases of hepatic hemangioma or hepatic injury tissues were also collectd. Expression of BMP-7 in HCC tissues(n = 85), adjacent tissues(n = 20), and non-cancerous liver tissues(n = 14) was analyzed by immunohistochemistry and RT-PCR. Relationship between BMP-7 and clinicopathological features was investigated. Results The positive expression rate of BMP-7 in hepatocellular carcinoma, adjacent tissues and non-cancerous liver tissue was significantly different. The expression of BMP-7 was closely correlated to the tumor size and differentiation degree of tumor tissue. Patients with positive expression of BMP-7 experienced poor outcome. Conclusion BMP-7 may play an important role in the carcinogenesis and progression of HCC, which may be an important prognostic indicator.
引文
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[12]郑涛,杨甲梅.转化生长因子β信号通路在肝癌患者中的作用机制[J].中华肝胆外科杂志,2016,22(6):425-428.
[2]I YA MATO,TTAK AYA MA,Y MIDORI K AWA,et al.Postoperative outcomes of patients with hepatocellular carcinoma negative for all virus-related markers[J].Surgery Today,2013,43(8):865-870.
[3]YANG M,FANG Z,YAN Z,et al.Transarterial chemoembolisation(TACE)combined with endovascular implantation of an iodine-125seed strand for the treatment of hepatocellular carcinoma with portal vein tumor thrombosis versus TACE alone:a two-arm,randomised clinical trial[J].Journal of Cancer Research and Clinical Oncology,2014,140(2):211-219.
[4]ZHEIRY M M,El-HASAN S A,AHWANY E,et al.Serum markers of epithelial mesenchymal transition as predictors of HCV-induced liver fibrosis,cirrhosis and hepatocellular carcinoma[J].Electron Physician,2015,7(8):1626-1637.
[5]ULRIKE M,ANJA-KATRIN B.BMP activated Smad signaling strongly promotes migration and invasion of hepatocellular carcinoma cells[J].Exp Mol Pathol,2012,92(1):74-81.
[6]HAN D,YBANEZ M D,JOHNSON H S,et al.Dynamic adaptation of livermitochondria to chronic alcohol feeding in mice:biogenesis,remode-ling,and functional alterations[J].J Biol Chem,2012,287(50):42165-42179.
[7]REN C M,LI Y,CHEN Q Z,et al.Oridonin inhibits the proliferation of human colon cancer cells by upregulating BMP-7to activate p38 MAPK[J].Oncol Rep,2016,35(5):2691-2698.
[8]朱振红,巨卫平,李伟.BMP-7在胃癌组织中的表达及临床意义[J].徐州医学院学报,2014,34(12):843-846.
[9]MF AKDOGAN,A AZAK,B HUDDAM,et al.Influence of bone morphogenic protein 7(BMP-7)on the progression of chronic kidney disease[J].Journal of Nephrology&Urology Research,2014,2:1-8.
[10]ZHONG L,WANG X,WANG S,et al.The anti-fibrotic effect of bonemorphogenic protein-7(BMP-7)on liver fibrosis[J].Int JMed Sci,2013,10(4):441-450.
[11]SHU D Y,WOJCIECHOWSKI M C,LOVICU F J,et al.Bone morphogenetic protein-7 suppresses TGF-β2-induced epithelialmesenchymal transition in the lens:Implications for cataract prevention[J].Invest Ophthalmol Vis Sci,2017,58(2):781-796.
[12]郑涛,杨甲梅.转化生长因子β信号通路在肝癌患者中的作用机制[J].中华肝胆外科杂志,2016,22(6):425-428.