深海鲣鱼鱼油对D-半乳糖致衰老模型小鼠抗衰老的作用
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摘要
以D-半乳糖致衰老模型小鼠为研究对象,评价深海鲣鱼鱼油对衰老模型小鼠抗衰老的作用。以健康育龄ICR小鼠为实验对象,随机分成6组,每组8只,即空白对照组,鲣鱼鱼油制剂低、中、高剂量组(20、50、100 mg/mL),D-半乳糖模型组和阳性对照组。采用皮下注射方式,空白对照组小鼠每只每天注射质量分数0.9%的生理盐水,其余实验组注射质量分数为4%的D-半乳糖(1 000 mg/kg m_b),同时,鲣鱼鱼油制剂组分别每天灌胃10 mL/kg m_b不同质量浓度(20、50、100 mg/mL)的鱼油制剂,阳性组灌胃市售鱼油(100 mg/kg m_b),空白对照组和模型组灌胃等体积生理盐水,持续6周。随后检测各组小鼠肝、脾指数,肝脏组织和血清的谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、过氧化氢酶(catalase,CAT)活力及丙二醛(malondialdehyde,MDA)水平等指标,制备肝脏切片,观察小鼠肝脏组织的病理变化情况。结果表明:与模型组相比,鲣鱼鱼油高剂量组和空白对照组的肝、脾指数均呈显著下降趋势(P<0.05);模型组小鼠血清和肝组织GSH-Px、T-SOD、CAT活力较空白对照组显著降低,MDA水平显著升高(P<0.05),鲣鱼鱼油各剂量组均能提高血清和肝组织中GSH-Px、T-SOD、CAT活力并降低MDA水平;通过切片观察,深海鲣鱼鱼油可缓解衰老过程中肝脏的损伤,并且高剂量组的效果最为明显。
The objective of this research was to evaluate the anti-aging effect of skipjack tuna oil(STO) on a mouse model of D-galactose-induced aging. Healthy ICR mice of reproductive age were randomly divided into 6 groups(n = 8): blank control, low-,middle-and high-dose STO, aging model and positive control groups. Mice in the control group were subjected to subcutaneous injection of 0.9% saline every day, while mice in other experimental groups were subjected to subcutaneous injection of 4%D-galactose(1 000 mg/kg m_b) and administrated with different concentrations of STO(20, 50 and 100 mg/m L) at a dose of10 mL/kg m_b by gavage. Mice in the positive control group were administrated with commercial fish oil(100 mg/kg m_b)and those in the blank control and aging model groups were by gavage administrated with an equal volume of normal saline for 6 weeks. The activity of glutathione peroxidase(GSH-Px), total superoxide dismutase(T-SOD) and catalase(CAT), and the content of malondialdehyde(MDA) in the liver and serum of mice were determined. The pathological changes of liver tissues were observed. Results indicated that the liver and spleen indexes in the high-dose and blank control groups were significantly lower than in the model group(P < 0.05). The serum and hepatic activities of GSH-Px,T-SOD and CAT in the model group were signi?cantly decreased relative to the blank control group(P < 0.05) while the opposite was found for MDA content. STO at all doses increased the levels of GSH-Px, T-SOD and CAT in serum and liver tissue and reduced MDA level. Pathological observation showed that STO could alleviate liver damage during aging,especially at high dose.
The objective of this research was to evaluate the anti-aging effect of skipjack tuna oil(STO) on a mouse model of D-galactose-induced aging. Healthy ICR mice of reproductive age were randomly divided into 6 groups(n = 8): blank control, low-,middle-and high-dose STO, aging model and positive control groups. Mice in the control group were subjected to subcutaneous injection of 0.9% saline every day, while mice in other experimental groups were subjected to subcutaneous injection of 4%D-galactose(1 000 mg/kg m_b) and administrated with different concentrations of STO(20, 50 and 100 mg/m L) at a dose of10 mL/kg m_b by gavage. Mice in the positive control group were administrated with commercial fish oil(100 mg/kg m_b)and those in the blank control and aging model groups were by gavage administrated with an equal volume of normal saline for 6 weeks. The activity of glutathione peroxidase(GSH-Px), total superoxide dismutase(T-SOD) and catalase(CAT), and the content of malondialdehyde(MDA) in the liver and serum of mice were determined. The pathological changes of liver tissues were observed. Results indicated that the liver and spleen indexes in the high-dose and blank control groups were significantly lower than in the model group(P < 0.05). The serum and hepatic activities of GSH-Px,T-SOD and CAT in the model group were signi?cantly decreased relative to the blank control group(P < 0.05) while the opposite was found for MDA content. STO at all doses increased the levels of GSH-Px, T-SOD and CAT in serum and liver tissue and reduced MDA level. Pathological observation showed that STO could alleviate liver damage during aging,especially at high dose.
引文
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[15]卢春雪,杨绍杰,陶荟竹,等.衰老机制研究进展[J].中国老年学杂志, 2018, 38(1): 248-250. DOI:10.3969/j.issn.1005-9202.2018.01.101.
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[17]丁秋英.金福菇多糖抗衰老作用研究[D].合肥:安徽大学, 2016:55-59.
[18]李妍,王静,李麒龙,等.E PA与D H A最新研究进展[J].农产品加工(学刊), 2013, 8(2): 6-13. DOI:10.3969/j.issn.1671-9646(X).2013.02.002.
[19] GARRARD O N. Omega 3 fatty acids and cardiovascular disease[J]. Journal of the American College of Cardiology, 2011, 58(20): 2047-2067. DOI:10.1016/j.jacc.2011.06.063.
[20] HOOPER L, THOMPSON R L, HARRISON R A, et al. Omega 3 fatty acids for prevention and treatment of cardiovascular disease[J]. Cochrane Database of Systematic Reviews, 2004, 4(4): 1-171. DOI:10.1002/14651858.CD003177.
[21]石雨,田媛,李磊,等.E PA、 D H A的生理功能及提取方法的研究进展[J].黑龙江科学, 2014, 5(10): 24-25. DOI:10.3969/j.issn.1674-8646.2014.10.007.
[22]王霞,黄健,李妍妍,等.金枪鱼鱼油对D-半乳糖致衰老小鼠学习记忆的作用[J].中国食品学报, 2012, 12(10): 24-28.
[23]高健美,牛爽,李海波.金丝桃苷对D-半乳糖衰老模型小鼠的抗衰老作用[J].中药药理与临床, 2017, 33(1): 57-59. DOI:10.13412/j.cnki.zyyl.2017.01.016.
[24]陈燏.活性肽-N对D-半乳糖致衰老小鼠作用效果研究[D].南宁:广西大学, 2017:62-66.
[25]陈宁园.火麻仁提取液对D-半乳糖致衰老大鼠空间学习和记忆的干预作用及其机制研究[D].南宁:广西医科大学, 2017:26-30.
[26]王敏君.肝细胞的衰老特性及衰老逆转的研究[D].上海:第二军医大学, 2014:16-22.
[27]李庆林,肖能坎,张琳.小鼠创伤愈合新生皮肤组织切片的制备[J].中外医疗, 2008, 28(10): 45-46. DOI:10.3969/j.issn.1674-0742.2008.10.035.
[28]张罕思.大豆皂苷对D-半乳糖所致衰老小鼠肝脏抗氧化作用的影响[D].延吉:延边大学, 2016:18-22.
[29]赵静.南极磷虾磷脂提取纯化及抗衰老实验研究[D].青岛:中国海洋大学, 2012:46-48.
[30]段文明.玉郎伞多糖抗衰老作用及机制研究[D].南宁:广西医科大学, 2014:23-28.
[31]张罕思.大豆皂苷对D-半乳糖所致衰老小鼠肝脏抗氧化作用的影响[D].延吉:延边大学, 2016:28-32.
[32]朱文娟.体外培养BMSCs联合补肾生髓法延缓D-半乳糖模型大鼠衰老的研究[D].长沙:湖南中医药大学, 2016:32-36.
[2]陈洋洋,陈新军.中西太平洋鲣鱼渔业研究进展[J].广东海洋大学学报, 2017, 37(5): 34-43.
[3]朱曙光,倪震宇.中西太平洋金枪鱼渔业现状及中国发展对策[J].中国渔业经济, 2017, 35(4): 27-33. DOI:10.3969/j.issn.1009-590X.2017.04.005.
[4]全晶晶,蔡江佳,郑平安,等.鲣鱼肌肉品质改良研究[J].中国食品学报, 2013, 13(7): 122-128. DOI:10.16429/j.1009-7848.2013.07.024.
[5]王婷.鲣鱼软罐头食品的加工技术研究[D].舟山:浙江海洋大学,2016:86-90.
[6]VIEIRA H C, BORDALO, MORGADO F, et al. Mercury content in the white and dark muscle of Skipjack tuna (Katsuwonus pelamis) along the canning process: implications to the consumers[J]. Journal of Food Composition & Analysis, 2017, 56(56): 67-72. DOI:10.1016/j.jfca.2016.11.011.
[7]高颐雄.舟山地区海水鱼类消费对健康影响的风险-获益评估研究[D].北京:中国疾病预防控制中心, 2013:65-68.
[8]李海波,吕倩倩,白冬,等.深海鲣鱼(Katsuwonus pelamis)眼窝油中和的提取及其抗抑郁活性研究[J].海洋与湖沼, 2016, 47(6): 1214-1220.
[9] NIKOLIC N, JéR?ME M, FONTENEAU A, et al. Identification of skipjack tuna juveniles based on DNA control region sequences and potential spawning area around reunion island[J]. Environmental Biology of Fishes, 2016, 99(2/3): 171-178. DOI:10.1007/s10641-015-0464-7.
[10]周敏,陶宁萍,王锡昌.黄鳍金枪鱼眼窝油中DHA含量测定方法的优化[J].湖南农业科学, 2010, 46(6): 93-95. DOI:10.3969/j.issn.1006-060X.2010.11.031.
[11]丛敬,吴景东,苏秋香.黄芪延缓小鼠皮肤衰老的实验研究[J].中国美容医学杂志, 2010, 19(2): 209-211. DOI:10.3969/j.issn.1008-6455.2010.02.028.
[12]刘景峰,王淑娟.现代医学衰老机理研究[J].辽宁中医药学院学报,2002, 4(2): 106-107. DOI:10.3969/j.issn.1673-842X.2002.02.014.
[13] GOTO M, IWAKI-EGAWA S, WATANABE Y. Ageing in Werner syndrome[J]. Bioscience Trends, 2012, 6(1): 33-37. DOI:10.5582/bst.2012.v6.1.33.
[14]周玉枝,闫明亮,高丽,等.衰老动物模型的研究及其在抗衰老药物活性筛选中的应用[J].中草药, 2017, 48(6): 1061-1071. DOI:10.7501/j.issn.0253-2670.2017.06.002.
[15]卢春雪,杨绍杰,陶荟竹,等.衰老机制研究进展[J].中国老年学杂志, 2018, 38(1): 248-250. DOI:10.3969/j.issn.1005-9202.2018.01.101.
[16]CRAWFORDM A.Theroleofessentialfattyacidsinneural development: implications for perinatal nutrition[J]. American Journal of Clinical Nutrition, 1993, 57(Suppl 5): 703-709. DOI:10.1093/ajcn/57.5.703S.
[17]丁秋英.金福菇多糖抗衰老作用研究[D].合肥:安徽大学, 2016:55-59.
[18]李妍,王静,李麒龙,等.E PA与D H A最新研究进展[J].农产品加工(学刊), 2013, 8(2): 6-13. DOI:10.3969/j.issn.1671-9646(X).2013.02.002.
[19] GARRARD O N. Omega 3 fatty acids and cardiovascular disease[J]. Journal of the American College of Cardiology, 2011, 58(20): 2047-2067. DOI:10.1016/j.jacc.2011.06.063.
[20] HOOPER L, THOMPSON R L, HARRISON R A, et al. Omega 3 fatty acids for prevention and treatment of cardiovascular disease[J]. Cochrane Database of Systematic Reviews, 2004, 4(4): 1-171. DOI:10.1002/14651858.CD003177.
[21]石雨,田媛,李磊,等.E PA、 D H A的生理功能及提取方法的研究进展[J].黑龙江科学, 2014, 5(10): 24-25. DOI:10.3969/j.issn.1674-8646.2014.10.007.
[22]王霞,黄健,李妍妍,等.金枪鱼鱼油对D-半乳糖致衰老小鼠学习记忆的作用[J].中国食品学报, 2012, 12(10): 24-28.
[23]高健美,牛爽,李海波.金丝桃苷对D-半乳糖衰老模型小鼠的抗衰老作用[J].中药药理与临床, 2017, 33(1): 57-59. DOI:10.13412/j.cnki.zyyl.2017.01.016.
[24]陈燏.活性肽-N对D-半乳糖致衰老小鼠作用效果研究[D].南宁:广西大学, 2017:62-66.
[25]陈宁园.火麻仁提取液对D-半乳糖致衰老大鼠空间学习和记忆的干预作用及其机制研究[D].南宁:广西医科大学, 2017:26-30.
[26]王敏君.肝细胞的衰老特性及衰老逆转的研究[D].上海:第二军医大学, 2014:16-22.
[27]李庆林,肖能坎,张琳.小鼠创伤愈合新生皮肤组织切片的制备[J].中外医疗, 2008, 28(10): 45-46. DOI:10.3969/j.issn.1674-0742.2008.10.035.
[28]张罕思.大豆皂苷对D-半乳糖所致衰老小鼠肝脏抗氧化作用的影响[D].延吉:延边大学, 2016:18-22.
[29]赵静.南极磷虾磷脂提取纯化及抗衰老实验研究[D].青岛:中国海洋大学, 2012:46-48.
[30]段文明.玉郎伞多糖抗衰老作用及机制研究[D].南宁:广西医科大学, 2014:23-28.
[31]张罕思.大豆皂苷对D-半乳糖所致衰老小鼠肝脏抗氧化作用的影响[D].延吉:延边大学, 2016:28-32.
[32]朱文娟.体外培养BMSCs联合补肾生髓法延缓D-半乳糖模型大鼠衰老的研究[D].长沙:湖南中医药大学, 2016:32-36.