茶多酚高通量检测技术研究进展
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摘要
茶多酚为茶叶主要活性成分,实现其组分的高通量快速检测,对茶叶加工品质控制和产品质量评价具有重要指导意义。对茶多酚高通量快速检测技术及其相关应用进行了归纳和总结,详细阐述了高效液相色谱法及其影响因素(色谱柱、检测器、样品前处理、流动相、洗脱方式),亦对毛细管电泳法、近红外光谱法、核磁共振法等检测方法进行简述,并分析各方法的优势及存在问题。研究认为,茶多酚高通量检测方法仍普遍存在程序繁琐、设备成本高昂、有机溶剂耗量大、检测灵敏度低等问题,在此基础提出液质联用技术的产业化发展,同时经济环保的实时在线监测以及复杂基质中茶多酚组分的快速定性定量是茶多酚高通量检测技术未来重点发展方向。
Tea polyphenols are the main active ingredients of tea. The high throughput and rapid test of tea polyphenols has guiding significance for tea processing quality control and tea quality evaluation. So, the study reviewed the high throughput test technique and its application to tea polyphenols, described the high performance liquid chromatography and its influencing factors(such as column, detector, sample pretreatment,mobile phase and elution method), and briefed other detection methods including capillary electrophoresis,near infrared spectroscopy and nuclear magnetic resonance. Additionally, the study analyzed the advantages and existing problems of these methods. The study indicated that the high throughput test of tea polyphenols still had some problems such as complex procedures, high cost, high organic solvent consumption, low sensitivity and so on. Based on the analysis, the study proposes the industrialization development of liquidmass technology, and points out that economical and environment-friendly real-time online monitoring, and rapid qualitative and quantitative analysis of tea polyphenols in complex substrates are the key development direction of the high throughput test of tea polyphenols.
Tea polyphenols are the main active ingredients of tea. The high throughput and rapid test of tea polyphenols has guiding significance for tea processing quality control and tea quality evaluation. So, the study reviewed the high throughput test technique and its application to tea polyphenols, described the high performance liquid chromatography and its influencing factors(such as column, detector, sample pretreatment,mobile phase and elution method), and briefed other detection methods including capillary electrophoresis,near infrared spectroscopy and nuclear magnetic resonance. Additionally, the study analyzed the advantages and existing problems of these methods. The study indicated that the high throughput test of tea polyphenols still had some problems such as complex procedures, high cost, high organic solvent consumption, low sensitivity and so on. Based on the analysis, the study proposes the industrialization development of liquidmass technology, and points out that economical and environment-friendly real-time online monitoring, and rapid qualitative and quantitative analysis of tea polyphenols in complex substrates are the key development direction of the high throughput test of tea polyphenols.
引文
[1] Kanwar J, Taskeen M, Mohammad I, et al. Recent advances on tea polyphenols[J]. Frontiers in Bioscience,2012,4(4):111-131.
[2] Camouse M M, Hanneman K K, Conrad E P, et al. Protective effects of tea polyphenols and caffeine[J]. Expert Review of Antiinfective Therapy,2014,5(6):1061-1068.
[3] Afzal M, Safer A M, Menon M. Green tea polyphenols and their potential role in health and disease[J]. Inflammopharmacology,2015,23(4):151-161.
[4] Yang C S, Lambert J D, Sang S. Antioxidative and anticarcinogenic activities of tea polyphenols[J]. Archives of Toxicology,2009,83(1):11-21.
[5] Yung L M, Leung F P, Wong W T, et al. Tea polyphenols benefit vascular function[J]. Inflammopharmacology,2008,16(5):230-234.
[6] Viapiana A, Wesolowski M. The Phenolic Contents and Antioxidant Activities of Infusions of Sambucus nigra[J]. Plant Foods Hum Nutr,2017,72(1):82-87.
[7] Huo C, Wan S B, Lam W H, et al. The challenge of developing green tea polyphenols as therapeutic agents[J].Inflammopharmacology,2008,16(5):248-252.
[8] Liang J, Yan H, Puligundla P, et al. Applications of chitosan nanoparticles to enhance absorption and bioavailability of tea polyphenols:A review[J]. Food Hydrocolloids,2017,69:286-292.
[9]祁洁,徐颖磊,梁文怡,等.EGCG纳米载体制备技术及其对EGCG活性影响的研究进展[J].茶叶科学,2017,37(2):119-129.
[10]朱丽萍,颜世敢.动物病原高通量检测技术[J].动物医学进展,2011,32(10):103-106.
[11] Lambert M, Meudec E, Verbaere A, et al. A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rose Wines[J]. Molecules,2015,20(5):7890-7914.
[12] Zhang S, Huang M T, Jiao Y J, et al. Rapid and High Throughput Quantitative Analysis of Catechin Polyphenols in Tea Beverages by Ultra-High Performance Liquid Chromatography with Photodiode ArrayDetection(UPLC-PDA)[J].Food Science,2013,34(22):170-173.
[13] Perisvicente J, Ramblaalegre M, Durgavanshi A, et al. Xanthine derivatives quantification in serum by capillary zone electrophoresis[J]. Journal of Chromatographic Science,2014,52(9):1121-1126.
[14] Guillarme D, Casetta C, Bicchi C, et al. High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors[J].Journal of Chromatography A,2010,1217(44):6882-6890
[15] Yang P P, Zhou H Y, Cong X D, et al. Rapid determination of geniposide content during gardenia countercurrent extract process through near infrared spectroscopy[J]. Nanjing Univ Tradit Chin Med,2014,30(1):72-75.
[16] Hoefler A C, Coggon P. Reversed-phase high-performance liquid chromatography of tea constituents[J]. Journal of Chromatography A,1976,129(12):460-463.
[17] Dalluge J J, Nelson B C, Thomas J B, et al. Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography[J]. Journal of Chromatography A,1998,793(2):265-274.
[18] Nanjegowda S H, Papanna M G, Achar R R, et al. A gradient based facile HPLC method for simultaneous estimation of antioxidants extracted from tea powder[J]. Journal of Food Science and Technology,2016,53(5):2253-2259.
[19]王丽丽,陈键,宋振硕,等.茶叶中没食子酸、儿茶素类和生物碱的HPLC检测方法研究[J].福建农业学报,2014,29(10):987-994.
[20] Dalluge J J, Nelson B C, Thomas J B, et al. Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography[J]. Journal of Chromatography A,1998,793(2):265-274.
[21] Ortega N, Romero M P, MaciàA, et al. Comparative study of UPLC–MS/MS and HPLC–MS/MS to determine procyanidins and alkaloids in cocoa samples[J]. Journal of Food Composition&Analysis,2010,23(3):298-305.
[22]郭颖,陈琦,黄峻榕,等.超高效液相色谱法测定茶叶中没食子酸、咖啡碱和儿茶素含量[J].食品科技,2015,40(11):296-300.
[23] Qi P, Zeng T, Wen Z, et al. Interference-free simultaneous determination of Sudan dyes in chili foods using solid phase extraction coupled with HPLC-DAD[J]. Food Chemistry,2011,125(4):1462-1467.
[24] Liu H, Tong H. Determination of Ten Components in Congou Black Tea by HPLC[J]. Food Science,2016,37(8):97-101.
[25] Wang Y, Yang X, Li K, et al. Simultaneous determination of theanine, gallic acid, purine alkaloids, catechins, and theaflavins in black tea using HPLC[J]. International Journal of Food Science&Technology,2010,45(6):1263-1269.
[26] Fu T, Liang J, Han G, et al. Simultaneous determination of the major active components of tea polyphenols in rat plasma by a simple and specific HPLC assay[J]. Journal of Chromatography B Analytical Technologies in the Biomedical&Life Sciences,2008,875(2):363-367.
[27]凌云.多组分茶儿茶素HPLC分析方法的建立及人体吸收与利用研究[D].北京:中国疾病预防控制中心,2005.
[28] Samanidou V, Tsagiannidis A, Sarakatsianos I. Simultaneous determination of polyphenols and major purine alkaloids in Greek Sideritis species, herbal extracts, green tea, black tea and coffee by high-performance liquid chromatography-diode array detection[J].Journal of Separation Science,2012,35(4):608-615.
[29]李程程,梁丽娜,于光前.高效液相色谱法测定砖茶中儿茶素和生物碱含量的研究[J].中华地方病学杂志,2013,32(4):453-457.
[30]鹿洋.茶多酚的提取纯化及其性质研究[D].天津:天津大学,2013.
[31]黄丽凤,刘友平,黎代余.茶多酚提取纯化工艺研究进展[J].中国食品添加剂,2010,11(1):69-72.
[32] Choung M G, Lee M S. Optimal extraction conditions for simultaneous determination of catechins and caffeine in green tea leaves[J]. Food Science and Biotechnology,2011,20(2):327-333.
[33]张帅.茶叶中儿茶素的提取研究[J].安徽农业科学,2007,35(30):39.
[34] Yin-Hua L I, Juan L I, Gong X, et al. Simultaneous Determination of Eight Catechins, Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography[J]. Food Science,2011,32(18):214-217.
[35]张国民,廖予琦,王庆忠.HPLC内标标准曲线法测定茶叶中4种儿茶素和咖啡因[J].昆明学院学报,2010,32(3):47-50.
[36]郭颖,黄峻榕,陈琦,等.茶叶中儿茶素类测定方法的优化[J].食品科学,2016,37(6):137-141.
[37]康海宁,陈波,韩超,等.HPLC法测定茶叶水提液中五种儿茶素和咖啡碱及其用于茶叶分类的研究[J].分析测试学报,2007,26(2):211-215.
[38]胡燕.不同产地黑茶的化学指纹图谱研究[D].雅安:四川农业大学,2014.
[39]刘丽霞.茶叶中6种主要儿茶素的高效液相色谱方法建立及应用[D].南京:南京理工大学,2013.
[40] Zhang Q, Wei-Ling X U, Cui-Qin L I. Simultaneous determination of some selected catechins and caffeine in tea by performance liquid chromatography[J]. Science&Technology of Food Industry,2015,36(4):53-56.
[41] Qiang H, Lv Y P, Ling Z, et al. Simultaneous determination of caffeine and catechins in tea extracts by HPLC[J]. Journal of Liquid Chromatography&Related Technologies,2010,33(4):491-498.
[42]吕丹,莫丽,张丽萍,等.浙江产15种茶叶中5种儿茶素的含量测定[J].时珍国医国药,2014,25(7):1567-1569.
[43]邵自星,谢媛媛,罗国安,等.同时测定乌龙茶中咖啡因和5种儿茶素类成分的方法研究[J].中国药学杂志,2014,49(24):2201-2205.
[44] Hu B, Wang L, Zhou B, et al. Efficient procedure for isolating methylated catechins from green tea and effective simultaneous analysis of ten catechins, three purine alkaloids and gallic acid in tea by high-performance liquid chromatography with diode array detection[J]. Journal of Chromatography A,2009,1216(15):3223-3231.
[45]夏文娟.毛细管电泳分析茶黄素类和茶多酚的方法与应用研究[D].山东:山东农业大学,2006.
[46] Watanabe T, Nishiyama R, Yamamoto A, et al. Simultaneous Analysis of Individual Catechins, Caffeine, and Ascorbic Acid in Commercial Canned Green and Black Teas by Micellar Electrokinetic Chromatography[J]. Analytical Sciences,1998,14(2):435-438.
[47] Aucamp J P, Hara Y, Apostolides Z. Simultaneous analysis of tea catechins, caffeine, gallic acid, theanine and ascorbic acid by micellar electrokinetic capillary chromatography[J]. Journal of Chromatography A,2000,876(1-2):235-242.
[48] Chen Q, Zhao J, Huang X, et al. Simultaneous determination of total polyphenols and caffeine contents of green tea by near-infrared reflectance spectroscopy[J]. Microchemical Journal,2006,83(1):42-47.
[49] Lee M S, Hwang Y S, Lee J, et al. The characterization of caffeine and nine individual catechins in the leaves of green tea(Camellia sinensis L.)by near-infrared reflectance spectroscopy[J]. Food Chemistry,2014,158(11):351-357.
[50] Li X, Luo L, He Y, et al. Determination of dry matter content of tea by near and middle infrared spectroscopy coupled with waveletbased data mining algorithms[J]. Computers&Electronics in Agriculture,2013,98(98):46-53.
[51] Pan W, Zhao J, Chen Q, et al. Simultaneous and Rapid Measurement of Main Compositions in Black Tea Infusion Using a Developed Spectroscopy System Combined with Multivariate Calibration[J]. Food Analytical Methods,2015,8(3):749-757.
[52]张烨,刘小鹏,张友杰.水溶性维生素核磁共振定量分析的研究[J].波谱学杂志,2011,28(3):357-365.
[53]陈波,张巍,康海宁,等.茶叶的1HNMR指纹图谱研究[J].波谱学杂志,2006,23(2):169-180.
[54]方萍,沈佳佳,鲁越青.5类茶叶总组分的核磁氢谱指纹图谱研究[J].绍兴文理学院学报,2012,32(7):61-66.
[55] Xiao W B, Yang J, Hua L I. Application of Two-dimensional Liquid Chromatography in Bioanalysis of Drugs and Toxicants[J].Chinese Journal of Analytical Chemistry,2014,42(12):1851-1858.
[56] Kula M, G?ód D, Krauzebaranowska M. Two-dimensional liquid chromatography(LC)of phenolic compounds from the shoots of Rubus idaeus ‘Glen Ample’ cultivar variety[J]. Journal of Pharmaceutical&Biomedical Analysis,2016,121:99-106.
[2] Camouse M M, Hanneman K K, Conrad E P, et al. Protective effects of tea polyphenols and caffeine[J]. Expert Review of Antiinfective Therapy,2014,5(6):1061-1068.
[3] Afzal M, Safer A M, Menon M. Green tea polyphenols and their potential role in health and disease[J]. Inflammopharmacology,2015,23(4):151-161.
[4] Yang C S, Lambert J D, Sang S. Antioxidative and anticarcinogenic activities of tea polyphenols[J]. Archives of Toxicology,2009,83(1):11-21.
[5] Yung L M, Leung F P, Wong W T, et al. Tea polyphenols benefit vascular function[J]. Inflammopharmacology,2008,16(5):230-234.
[6] Viapiana A, Wesolowski M. The Phenolic Contents and Antioxidant Activities of Infusions of Sambucus nigra[J]. Plant Foods Hum Nutr,2017,72(1):82-87.
[7] Huo C, Wan S B, Lam W H, et al. The challenge of developing green tea polyphenols as therapeutic agents[J].Inflammopharmacology,2008,16(5):248-252.
[8] Liang J, Yan H, Puligundla P, et al. Applications of chitosan nanoparticles to enhance absorption and bioavailability of tea polyphenols:A review[J]. Food Hydrocolloids,2017,69:286-292.
[9]祁洁,徐颖磊,梁文怡,等.EGCG纳米载体制备技术及其对EGCG活性影响的研究进展[J].茶叶科学,2017,37(2):119-129.
[10]朱丽萍,颜世敢.动物病原高通量检测技术[J].动物医学进展,2011,32(10):103-106.
[11] Lambert M, Meudec E, Verbaere A, et al. A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rose Wines[J]. Molecules,2015,20(5):7890-7914.
[12] Zhang S, Huang M T, Jiao Y J, et al. Rapid and High Throughput Quantitative Analysis of Catechin Polyphenols in Tea Beverages by Ultra-High Performance Liquid Chromatography with Photodiode ArrayDetection(UPLC-PDA)[J].Food Science,2013,34(22):170-173.
[13] Perisvicente J, Ramblaalegre M, Durgavanshi A, et al. Xanthine derivatives quantification in serum by capillary zone electrophoresis[J]. Journal of Chromatographic Science,2014,52(9):1121-1126.
[14] Guillarme D, Casetta C, Bicchi C, et al. High throughput qualitative analysis of polyphenols in tea samples by ultra-high pressure liquid chromatography coupled to UV and mass spectrometry detectors[J].Journal of Chromatography A,2010,1217(44):6882-6890
[15] Yang P P, Zhou H Y, Cong X D, et al. Rapid determination of geniposide content during gardenia countercurrent extract process through near infrared spectroscopy[J]. Nanjing Univ Tradit Chin Med,2014,30(1):72-75.
[16] Hoefler A C, Coggon P. Reversed-phase high-performance liquid chromatography of tea constituents[J]. Journal of Chromatography A,1976,129(12):460-463.
[17] Dalluge J J, Nelson B C, Thomas J B, et al. Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography[J]. Journal of Chromatography A,1998,793(2):265-274.
[18] Nanjegowda S H, Papanna M G, Achar R R, et al. A gradient based facile HPLC method for simultaneous estimation of antioxidants extracted from tea powder[J]. Journal of Food Science and Technology,2016,53(5):2253-2259.
[19]王丽丽,陈键,宋振硕,等.茶叶中没食子酸、儿茶素类和生物碱的HPLC检测方法研究[J].福建农业学报,2014,29(10):987-994.
[20] Dalluge J J, Nelson B C, Thomas J B, et al. Selection of column and gradient elution system for the separation of catechins in green tea using high-performance liquid chromatography[J]. Journal of Chromatography A,1998,793(2):265-274.
[21] Ortega N, Romero M P, MaciàA, et al. Comparative study of UPLC–MS/MS and HPLC–MS/MS to determine procyanidins and alkaloids in cocoa samples[J]. Journal of Food Composition&Analysis,2010,23(3):298-305.
[22]郭颖,陈琦,黄峻榕,等.超高效液相色谱法测定茶叶中没食子酸、咖啡碱和儿茶素含量[J].食品科技,2015,40(11):296-300.
[23] Qi P, Zeng T, Wen Z, et al. Interference-free simultaneous determination of Sudan dyes in chili foods using solid phase extraction coupled with HPLC-DAD[J]. Food Chemistry,2011,125(4):1462-1467.
[24] Liu H, Tong H. Determination of Ten Components in Congou Black Tea by HPLC[J]. Food Science,2016,37(8):97-101.
[25] Wang Y, Yang X, Li K, et al. Simultaneous determination of theanine, gallic acid, purine alkaloids, catechins, and theaflavins in black tea using HPLC[J]. International Journal of Food Science&Technology,2010,45(6):1263-1269.
[26] Fu T, Liang J, Han G, et al. Simultaneous determination of the major active components of tea polyphenols in rat plasma by a simple and specific HPLC assay[J]. Journal of Chromatography B Analytical Technologies in the Biomedical&Life Sciences,2008,875(2):363-367.
[27]凌云.多组分茶儿茶素HPLC分析方法的建立及人体吸收与利用研究[D].北京:中国疾病预防控制中心,2005.
[28] Samanidou V, Tsagiannidis A, Sarakatsianos I. Simultaneous determination of polyphenols and major purine alkaloids in Greek Sideritis species, herbal extracts, green tea, black tea and coffee by high-performance liquid chromatography-diode array detection[J].Journal of Separation Science,2012,35(4):608-615.
[29]李程程,梁丽娜,于光前.高效液相色谱法测定砖茶中儿茶素和生物碱含量的研究[J].中华地方病学杂志,2013,32(4):453-457.
[30]鹿洋.茶多酚的提取纯化及其性质研究[D].天津:天津大学,2013.
[31]黄丽凤,刘友平,黎代余.茶多酚提取纯化工艺研究进展[J].中国食品添加剂,2010,11(1):69-72.
[32] Choung M G, Lee M S. Optimal extraction conditions for simultaneous determination of catechins and caffeine in green tea leaves[J]. Food Science and Biotechnology,2011,20(2):327-333.
[33]张帅.茶叶中儿茶素的提取研究[J].安徽农业科学,2007,35(30):39.
[34] Yin-Hua L I, Juan L I, Gong X, et al. Simultaneous Determination of Eight Catechins, Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography[J]. Food Science,2011,32(18):214-217.
[35]张国民,廖予琦,王庆忠.HPLC内标标准曲线法测定茶叶中4种儿茶素和咖啡因[J].昆明学院学报,2010,32(3):47-50.
[36]郭颖,黄峻榕,陈琦,等.茶叶中儿茶素类测定方法的优化[J].食品科学,2016,37(6):137-141.
[37]康海宁,陈波,韩超,等.HPLC法测定茶叶水提液中五种儿茶素和咖啡碱及其用于茶叶分类的研究[J].分析测试学报,2007,26(2):211-215.
[38]胡燕.不同产地黑茶的化学指纹图谱研究[D].雅安:四川农业大学,2014.
[39]刘丽霞.茶叶中6种主要儿茶素的高效液相色谱方法建立及应用[D].南京:南京理工大学,2013.
[40] Zhang Q, Wei-Ling X U, Cui-Qin L I. Simultaneous determination of some selected catechins and caffeine in tea by performance liquid chromatography[J]. Science&Technology of Food Industry,2015,36(4):53-56.
[41] Qiang H, Lv Y P, Ling Z, et al. Simultaneous determination of caffeine and catechins in tea extracts by HPLC[J]. Journal of Liquid Chromatography&Related Technologies,2010,33(4):491-498.
[42]吕丹,莫丽,张丽萍,等.浙江产15种茶叶中5种儿茶素的含量测定[J].时珍国医国药,2014,25(7):1567-1569.
[43]邵自星,谢媛媛,罗国安,等.同时测定乌龙茶中咖啡因和5种儿茶素类成分的方法研究[J].中国药学杂志,2014,49(24):2201-2205.
[44] Hu B, Wang L, Zhou B, et al. Efficient procedure for isolating methylated catechins from green tea and effective simultaneous analysis of ten catechins, three purine alkaloids and gallic acid in tea by high-performance liquid chromatography with diode array detection[J]. Journal of Chromatography A,2009,1216(15):3223-3231.
[45]夏文娟.毛细管电泳分析茶黄素类和茶多酚的方法与应用研究[D].山东:山东农业大学,2006.
[46] Watanabe T, Nishiyama R, Yamamoto A, et al. Simultaneous Analysis of Individual Catechins, Caffeine, and Ascorbic Acid in Commercial Canned Green and Black Teas by Micellar Electrokinetic Chromatography[J]. Analytical Sciences,1998,14(2):435-438.
[47] Aucamp J P, Hara Y, Apostolides Z. Simultaneous analysis of tea catechins, caffeine, gallic acid, theanine and ascorbic acid by micellar electrokinetic capillary chromatography[J]. Journal of Chromatography A,2000,876(1-2):235-242.
[48] Chen Q, Zhao J, Huang X, et al. Simultaneous determination of total polyphenols and caffeine contents of green tea by near-infrared reflectance spectroscopy[J]. Microchemical Journal,2006,83(1):42-47.
[49] Lee M S, Hwang Y S, Lee J, et al. The characterization of caffeine and nine individual catechins in the leaves of green tea(Camellia sinensis L.)by near-infrared reflectance spectroscopy[J]. Food Chemistry,2014,158(11):351-357.
[50] Li X, Luo L, He Y, et al. Determination of dry matter content of tea by near and middle infrared spectroscopy coupled with waveletbased data mining algorithms[J]. Computers&Electronics in Agriculture,2013,98(98):46-53.
[51] Pan W, Zhao J, Chen Q, et al. Simultaneous and Rapid Measurement of Main Compositions in Black Tea Infusion Using a Developed Spectroscopy System Combined with Multivariate Calibration[J]. Food Analytical Methods,2015,8(3):749-757.
[52]张烨,刘小鹏,张友杰.水溶性维生素核磁共振定量分析的研究[J].波谱学杂志,2011,28(3):357-365.
[53]陈波,张巍,康海宁,等.茶叶的1HNMR指纹图谱研究[J].波谱学杂志,2006,23(2):169-180.
[54]方萍,沈佳佳,鲁越青.5类茶叶总组分的核磁氢谱指纹图谱研究[J].绍兴文理学院学报,2012,32(7):61-66.
[55] Xiao W B, Yang J, Hua L I. Application of Two-dimensional Liquid Chromatography in Bioanalysis of Drugs and Toxicants[J].Chinese Journal of Analytical Chemistry,2014,42(12):1851-1858.
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