抗氧化剂对家畜精液冷冻保存效果的影响研究进展
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摘要
家畜精液保存在畜牧生产实践中具有十分重要的意义,随着家畜人工授精技术的广泛应用,精液冷冻保存技术研究也更加深入。精液在冷冻保存的过程中,精子细胞会产生过量的活性氧,活性氧与精子细胞膜上的不饱和脂肪酸易发生氧化反应,破坏精子结构和功能的完整性,导致精液品质下降。抗氧化剂具有清除活性氧自由基的作用,可消除精液冷冻过程中所产生的过量活性氧,保护精子细胞免受氧化应激损伤,进而提高精液品质。本文主要对精子冷冻氧化性损伤机制及常用抗氧化剂进行综述,并对抗氧化剂的应用前景进行展望,以期为更进一步提高家畜精液冷冻保存品质,发挥良种优势提供理论依据与参考。
The preservation of livestock semen is of great significance in the practice of livestock production.With the extensive application of livestock artificial insemination,the research on cryopreservation of semen has become more in-depth.During the cryopreservation of semen,spermatozoa could produce excessive amounts of reactive oxygen species,causing oxidation reaction between sperm and reactive oxygen species,resulting in damage to the sperm structure and function,and eventually resulting in decreased semen quality.Antioxidants have the function of scavenging reactive oxygen species and free radicals,and can eliminate excess active oxygen generated,so that the spermatozoa are protected from oxidative stress,and improve the quality of semen.This review mainly summarizes the mechanism of oxidative damage in animal spermatozoa and commonly used antioxidants,and prospects for the application of antioxidants,hoping improve the cryopreservation quality of livestock semen and provide theoretical basis of superiority of thoroughbred livestock.
The preservation of livestock semen is of great significance in the practice of livestock production.With the extensive application of livestock artificial insemination,the research on cryopreservation of semen has become more in-depth.During the cryopreservation of semen,spermatozoa could produce excessive amounts of reactive oxygen species,causing oxidation reaction between sperm and reactive oxygen species,resulting in damage to the sperm structure and function,and eventually resulting in decreased semen quality.Antioxidants have the function of scavenging reactive oxygen species and free radicals,and can eliminate excess active oxygen generated,so that the spermatozoa are protected from oxidative stress,and improve the quality of semen.This review mainly summarizes the mechanism of oxidative damage in animal spermatozoa and commonly used antioxidants,and prospects for the application of antioxidants,hoping improve the cryopreservation quality of livestock semen and provide theoretical basis of superiority of thoroughbred livestock.
引文
[1]魏宽翠.奶牛体内胚胎采集与胚胎移植的技术研究[D].邯郸:河北工程大学,2013.
[2]Zribi N,Chakroun N F,Eleuch H,et al.Effects of cryopreservation on human spermdeoxyribonucleic acid integrity[J].Fertil Steril,2010,93:159-166.
[3]Zigo M,Dorosh A,Pohlova A,et al.Panel of monoclonal antibodies to spermsurface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors[J].Cell Tissue Res,2015,359(3):895-908.
[4]Abouelezz F M,Castano C,Toledano-Diaz A,et al.Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen/thawed chicken sperm variables[J].Reprod Domest Anim,2015,50(1):135-141.
[5]Gadea J,Molla M,Selles E,et al.Reduced glutathione content in human sperm is decreased after cryopreservation:effect of the addition of reduced glutathione to the freezing and thawing extenders[J].Cryobiology,2011,62:40-46.
[6]刘超,任有蛇,张春香,等.山羊精清中抗氧化酶活性与精子活力的相关性分析[J].中国草食动物,2010,30(3):20-23.
[7]Li Z L,Lin Q L,Liu R J,et al.Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation[J].Journal of Andrology,2010,31(5):437-444.
[8]王贞.几种抗氧化剂组合对猪精子冷冻保存效果的影响[D].雅安:四川农业大学,2010.
[9]权富生,黄欣,马会明,等.添加VB12,VE,VC对无角道塞特绵羊精液冷冻效果的影响[J].西北农业学报,2010,19(11):7-13.
[10]黄俻华,谢艺.精液稀释液中添加维生素C和E对陆川猪精液冷冻效果的影响[J].畜牧与兽医,2015,47(12):45-47.
[11]Hu J H,Zhao X L,Tian W Q,et al.Effects of vitamin E supplementation in the extender on frozenthawed bovine semen preservation[J].Animal,2011,5(1):104-107.
[12]赵宪林,昝林森,胡建宏,等.维生素E对秦川牛冷冻精液的抗氧化保护作用[J].畜牧兽医学报,2010,41(1):117-122.
[13]康孟阳.四种抗氧化剂在关中驴精液冷冻保存中的应用效果研究[D].杨凌:西北农林科技大学,2016.
[14]吴大平.不同抗氧化剂对地中海水牛精液冷冻保存效果的影响研究[D].南宁:广西大学,2017.
[15]Zhao X L,Li Y K,Cao S J,et al.Protective effects of ascorbic acid and vitamin E on antioxidant enzyme activity of freeze-thawed semen of Qinchuan bulls[J].Genetics and Molecular Research,2015,14(1):2572-2581.
[16]Serpil S K,Mustafa N B,Purhan B T,et al.The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation[J].Cryobiology,2009,58:134-138.
[17]范楚宁.半胱氨酸对獭兔精液冷冻保存效果的影响[D].杨凌:西北农林科技大学,2015.
[18]Khalili B M.The Effects of different concentrations of glycine and cysteine on the freezability of moghani ram spermatozoa[J].Asian-Aust,2010,23(3):318-325.
[19]Cheah I K,Halliwell B.Ergothioneine antioxidant potential physiological function and role in disease[J].Biochimicaet Biophysica Acta(BBA)-Molecular Basis of Disease,2012,1822(5):784-793.
[20]李轶群,周念波.麦角硫因的生物学功能及其应用[J].食品工程,2010,3(9):26-28.
[21]李亚欢.杏鲍菇中麦角硫因的提取纯化和抗氧化活性研究[D].广州:华南农业大学,2016.
[22]Coyan K,Bucak M N,Basp?nar N,et al.Ergothioneine attenuates the DNA damage of post-thawed Merino ram sperm[J].Small Ruminant Research,2012,1016:165-167.
[23]姜艳霞,纪朋艳,王莹,等.海带多糖对人肝癌bel-7402细胞存活和凋亡的影响[J].上海中医药杂志,2015,49(3):85-87.
[24]李西林,南艺雷.红景天多糖的化学药理研究进展[J].中国中医药信息杂志,2006,3(13):109-110.
[25]何涛,李军.海带多糖对猪精液冷冻效果影响的研究[J].畜牧与饲料科学,2013,34(1):23-25.
[26]张婷.四种糖类对猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2008.
[27]Hu J H,Sun X Z,Li Q W,et al.The effect of Laminaria japonic polysaccharide on sperm characteristics an biochemical parameters in cryopreserved boar sperm[J].Anim Reprod Sci,2013,139(1):95-100.
[28]陈西英.海带多糖对绵羊精液冷冻效果的作用[J].黑龙江畜牧兽医,2011,16(14):85-86.
[29]曹华琳.红景天和益母草多糖对牛猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2014.
[30]连星,张红俊,杜烨青,等.猪精液常温保存抗氧化剂的研究进展[J].畜牧兽医杂志,2017,36(4)41-43.
[31]Zeitoun M M,Al-Damegh M A.Effect of nonenzymatic antioxidants on sperm motility and survival relative to free radicals and antioxidant enzymes of chilled-stored ram semen[J].Open J Anim Sci,2014,5:50.
[32]段洪云,张翔,孙玉玲,等.马冷冻精液氧自由基的产生和清除系统[J].中国畜牧兽医,2013,40(2):133-136.
[33]Ma H M,Quan F S,Chen D M,et al.Protective function of alpha-lipoic acid on sperm motility and mitochondrial function during goat sperm-mediated gene transfer[J].Small Ruminant Research,2011,99(2-3):191-198.
[34]占今舜,张彬.浅谈褪黑素在动物生产中的应用[J].饲料博览,2011,(07):41-43.
[35]Amini P H,Tahmasbi A M,Naserian A A.The influence of vitamin E on semen characteristics of ghezel rams in during cooling and frozen process[J].Eur J Zool Res,.2013,2(5):94-99.
[36]杨宝卫,李美珍,黄明光,等.褪黑素与盐酸多巴胺在猪精液冷冻保存中的应用效果[J].贵州农业科学,2012,(08):154-155.
[37]Amidi F,Pazhohan A,Nzshtaei M S,et al.The role antioxidants in sperm freezing[J].Cell Tissue Bank,2016,1007(17):745-756.
[38]李博玲,马恒东,王贞,等.褪黑素和谷胱甘肽对猪精液冷冻保存效果的影响[J].四川畜牧兽医,2011,(7):26-28.
[2]Zribi N,Chakroun N F,Eleuch H,et al.Effects of cryopreservation on human spermdeoxyribonucleic acid integrity[J].Fertil Steril,2010,93:159-166.
[3]Zigo M,Dorosh A,Pohlova A,et al.Panel of monoclonal antibodies to spermsurface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors[J].Cell Tissue Res,2015,359(3):895-908.
[4]Abouelezz F M,Castano C,Toledano-Diaz A,et al.Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen/thawed chicken sperm variables[J].Reprod Domest Anim,2015,50(1):135-141.
[5]Gadea J,Molla M,Selles E,et al.Reduced glutathione content in human sperm is decreased after cryopreservation:effect of the addition of reduced glutathione to the freezing and thawing extenders[J].Cryobiology,2011,62:40-46.
[6]刘超,任有蛇,张春香,等.山羊精清中抗氧化酶活性与精子活力的相关性分析[J].中国草食动物,2010,30(3):20-23.
[7]Li Z L,Lin Q L,Liu R J,et al.Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation[J].Journal of Andrology,2010,31(5):437-444.
[8]王贞.几种抗氧化剂组合对猪精子冷冻保存效果的影响[D].雅安:四川农业大学,2010.
[9]权富生,黄欣,马会明,等.添加VB12,VE,VC对无角道塞特绵羊精液冷冻效果的影响[J].西北农业学报,2010,19(11):7-13.
[10]黄俻华,谢艺.精液稀释液中添加维生素C和E对陆川猪精液冷冻效果的影响[J].畜牧与兽医,2015,47(12):45-47.
[11]Hu J H,Zhao X L,Tian W Q,et al.Effects of vitamin E supplementation in the extender on frozenthawed bovine semen preservation[J].Animal,2011,5(1):104-107.
[12]赵宪林,昝林森,胡建宏,等.维生素E对秦川牛冷冻精液的抗氧化保护作用[J].畜牧兽医学报,2010,41(1):117-122.
[13]康孟阳.四种抗氧化剂在关中驴精液冷冻保存中的应用效果研究[D].杨凌:西北农林科技大学,2016.
[14]吴大平.不同抗氧化剂对地中海水牛精液冷冻保存效果的影响研究[D].南宁:广西大学,2017.
[15]Zhao X L,Li Y K,Cao S J,et al.Protective effects of ascorbic acid and vitamin E on antioxidant enzyme activity of freeze-thawed semen of Qinchuan bulls[J].Genetics and Molecular Research,2015,14(1):2572-2581.
[16]Serpil S K,Mustafa N B,Purhan B T,et al.The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation[J].Cryobiology,2009,58:134-138.
[17]范楚宁.半胱氨酸对獭兔精液冷冻保存效果的影响[D].杨凌:西北农林科技大学,2015.
[18]Khalili B M.The Effects of different concentrations of glycine and cysteine on the freezability of moghani ram spermatozoa[J].Asian-Aust,2010,23(3):318-325.
[19]Cheah I K,Halliwell B.Ergothioneine antioxidant potential physiological function and role in disease[J].Biochimicaet Biophysica Acta(BBA)-Molecular Basis of Disease,2012,1822(5):784-793.
[20]李轶群,周念波.麦角硫因的生物学功能及其应用[J].食品工程,2010,3(9):26-28.
[21]李亚欢.杏鲍菇中麦角硫因的提取纯化和抗氧化活性研究[D].广州:华南农业大学,2016.
[22]Coyan K,Bucak M N,Basp?nar N,et al.Ergothioneine attenuates the DNA damage of post-thawed Merino ram sperm[J].Small Ruminant Research,2012,1016:165-167.
[23]姜艳霞,纪朋艳,王莹,等.海带多糖对人肝癌bel-7402细胞存活和凋亡的影响[J].上海中医药杂志,2015,49(3):85-87.
[24]李西林,南艺雷.红景天多糖的化学药理研究进展[J].中国中医药信息杂志,2006,3(13):109-110.
[25]何涛,李军.海带多糖对猪精液冷冻效果影响的研究[J].畜牧与饲料科学,2013,34(1):23-25.
[26]张婷.四种糖类对猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2008.
[27]Hu J H,Sun X Z,Li Q W,et al.The effect of Laminaria japonic polysaccharide on sperm characteristics an biochemical parameters in cryopreserved boar sperm[J].Anim Reprod Sci,2013,139(1):95-100.
[28]陈西英.海带多糖对绵羊精液冷冻效果的作用[J].黑龙江畜牧兽医,2011,16(14):85-86.
[29]曹华琳.红景天和益母草多糖对牛猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学,2014.
[30]连星,张红俊,杜烨青,等.猪精液常温保存抗氧化剂的研究进展[J].畜牧兽医杂志,2017,36(4)41-43.
[31]Zeitoun M M,Al-Damegh M A.Effect of nonenzymatic antioxidants on sperm motility and survival relative to free radicals and antioxidant enzymes of chilled-stored ram semen[J].Open J Anim Sci,2014,5:50.
[32]段洪云,张翔,孙玉玲,等.马冷冻精液氧自由基的产生和清除系统[J].中国畜牧兽医,2013,40(2):133-136.
[33]Ma H M,Quan F S,Chen D M,et al.Protective function of alpha-lipoic acid on sperm motility and mitochondrial function during goat sperm-mediated gene transfer[J].Small Ruminant Research,2011,99(2-3):191-198.
[34]占今舜,张彬.浅谈褪黑素在动物生产中的应用[J].饲料博览,2011,(07):41-43.
[35]Amini P H,Tahmasbi A M,Naserian A A.The influence of vitamin E on semen characteristics of ghezel rams in during cooling and frozen process[J].Eur J Zool Res,.2013,2(5):94-99.
[36]杨宝卫,李美珍,黄明光,等.褪黑素与盐酸多巴胺在猪精液冷冻保存中的应用效果[J].贵州农业科学,2012,(08):154-155.
[37]Amidi F,Pazhohan A,Nzshtaei M S,et al.The role antioxidants in sperm freezing[J].Cell Tissue Bank,2016,1007(17):745-756.
[38]李博玲,马恒东,王贞,等.褪黑素和谷胱甘肽对猪精液冷冻保存效果的影响[J].四川畜牧兽医,2011,(7):26-28.