利用双酶法定向合成右旋糖酐现状研究
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摘要
右旋糖酐被广泛应用于医学、化工、食品等行业,是备受关注的糖类之一。其传统工艺是经微生物发酵所得,分子量排布较广,尤其中小分子量含量少,杂质较多,粘度大,后期处理过程繁琐。酶法合成多糖被认为是安全且有效的合成途径,但由于单酶合成所得的右旋糖酐分子量不易于控制,故文章以制备右旋糖酐为出发点,综述了双酶法定向合成右旋糖酐及其应用研究,为右旋糖酐的生产提供了新的理论支持。
Dextran is widely used in medicine,chemical engineering,food and other industries,and it is one of the most concerned sugar products.Dextran produced by traditional process methods with microbial fermentation has a wide distribution in the molecular weight,low content of medium and small molecular weight,and more impurities,high viscosity,complex late processing.Enzymatic synthesis of polysaccharides is found to be a safe and effective synthesis method.However,the molecular weight of dextran obtained by single enzyme synthesis is not easy to control,so that take preparation of dextran as the starting point,review the synthesis and application research of dextran using double enzymatic method,and provide theoretical support for the production of dextran.
Dextran is widely used in medicine,chemical engineering,food and other industries,and it is one of the most concerned sugar products.Dextran produced by traditional process methods with microbial fermentation has a wide distribution in the molecular weight,low content of medium and small molecular weight,and more impurities,high viscosity,complex late processing.Enzymatic synthesis of polysaccharides is found to be a safe and effective synthesis method.However,the molecular weight of dextran obtained by single enzyme synthesis is not easy to control,so that take preparation of dextran as the starting point,review the synthesis and application research of dextran using double enzymatic method,and provide theoretical support for the production of dextran.
引文
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[6]王雅洁,张洪斌,胡雪芹,等.重组大肠杆菌右旋糖酐蔗糖酶的纯化及其性质[J].微生物学报,2008,48(9):1266-1269.
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[9]姚日生,董明辉,朱慧霞.酶法合成右旋糖酐相对分子质量的控制[J].精细化工,2007,24(10):964-967.
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[14]Kim Y M,Seo M Y,Kang H K,et al.Construction of a fusion enzyme of dextransucrase and dextranase:application for one-step synthesis of isomalto-oligosaccharides[J].Enzyme and Microbial Technology,2009,44(3):159-164.
[15]Oelcer Z,Tanriseven A.Co-immobilization of dextransucrase and dextranase in alginate[J].Process Biochemistry(Amsterdam,Netherlands),2010,45(10):1645-1651.
[16]Tam S C,Blumenstein J,Wong J T.Soluble dextran-hemoglobin complex as a potential blood substitute[J].Proceedings of the National Academy of Sciences,1976,73(6):2128-2131.
[17]国家药典委员会.中华人民共和国药典(2010版,二部)[M].北京:中国医药科技出版社,2010:111-117.
[18]Krol A,Dewhirst M W,Yuan F.Effects of cell damage and glycosaminoglycan degradation on available extravascular space of different dextrans in a rat fibrosarcoma[J].International Journal of Hyperthermia,2003,19(2):154-164.
[19]Tomme S R,Nostrum C F,Smedt S C,et al.Degradation behavior of dextran hydrogels composed of positively and negatively charged microspheres[J].Biomaterials,2006,27(22):4141-4148.
[20]宋少云,廖威.葡聚糖的研究进展[J].中山大学学报(自然科学版),2005,44(s2):229-232.
[2]Robyt J F,Eklund S H.Relative,quantitative effects of acceptors in the reation of Leuconostoc mesenteroides B-512F dextransucrase[J].Carbohydrate Research,1983,121:279-286.
[3]Luzio G A,Mayer R M.The hydrolysis of sucrose by dextransucrase[J].Carbohydrate Research,1983,111(2):311-318.
[4]Larsson A M,Andersson R,Stahlberg J,et al.Dextranase from Penicillium minioluteum:reaction course,crystal structure,and product complex[J].Structure,2003,11(9):1111-1121.
[5]张洪斌,姚日生,朱慧霞,等.发酵法生产右旋糖酐的工艺研究[J].合肥工业大学学报(自然科学版),2004,27(7):783-787.
[6]王雅洁,张洪斌,胡雪芹,等.重组大肠杆菌右旋糖酐蔗糖酶的纯化及其性质[J].微生物学报,2008,48(9):1266-1269.
[7]Berensmeier S,Ergezinger M,Bohnet M,et al.Design of immobilised dextransucrase for fluidised bed application[J].Journal of Biotechnology,2004,114(3):255-267.
[8]Mohan R T J,Goyal A.Purification,optimization of assay,and stability studies of dextransucrase isolated from Weissella cibaria JAG8[J].Preparative Biochemistry and Biotechnology,2013,43(4):329-341.
[9]姚日生,董明辉,朱慧霞.酶法合成右旋糖酐相对分子质量的控制[J].精细化工,2007,24(10):964-967.
[10]Goulas A K,Fisher D A,Grimble G K,et al.Synthesis of isomaltooligosaccharides and oligodextrans by the combined use of dextransucrase and dextranase[J].Enzyme and Microbial Technology,2004,35(4):327-338.
[11]Kubik C,Sikora B,Bielecki S.Immobilization of dextransucrase and its use with soluble dextranase for glucooligosaccharides synthesis[J].Enzyme and Microbial Technology,2004,34(6):555-560.
[12]甘微苇.右旋糖酐蔗糖酶与右旋糖酐酶协同催化制备低聚右旋糖酐与低聚糖的研究[D].合肥:合肥工业大学,2014.
[13]Erhardt F A,Kugler J,Chakravarthula R R,et al.Co-immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto-oligosaccharides:preparation,characterization and modeling[J].Biotechnology and Bioengineering,2008,100(4):673-683.
[14]Kim Y M,Seo M Y,Kang H K,et al.Construction of a fusion enzyme of dextransucrase and dextranase:application for one-step synthesis of isomalto-oligosaccharides[J].Enzyme and Microbial Technology,2009,44(3):159-164.
[15]Oelcer Z,Tanriseven A.Co-immobilization of dextransucrase and dextranase in alginate[J].Process Biochemistry(Amsterdam,Netherlands),2010,45(10):1645-1651.
[16]Tam S C,Blumenstein J,Wong J T.Soluble dextran-hemoglobin complex as a potential blood substitute[J].Proceedings of the National Academy of Sciences,1976,73(6):2128-2131.
[17]国家药典委员会.中华人民共和国药典(2010版,二部)[M].北京:中国医药科技出版社,2010:111-117.
[18]Krol A,Dewhirst M W,Yuan F.Effects of cell damage and glycosaminoglycan degradation on available extravascular space of different dextrans in a rat fibrosarcoma[J].International Journal of Hyperthermia,2003,19(2):154-164.
[19]Tomme S R,Nostrum C F,Smedt S C,et al.Degradation behavior of dextran hydrogels composed of positively and negatively charged microspheres[J].Biomaterials,2006,27(22):4141-4148.
[20]宋少云,廖威.葡聚糖的研究进展[J].中山大学学报(自然科学版),2005,44(s2):229-232.