白菊‘精诚’离体快繁技术研究
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摘要
以日本独头白菊‘精诚’植株的顶芽、带腋芽茎段为外植体进行离体快繁研究。结果表明:以0.1%Hg Cl2为表面消毒剂,最适消毒时间为5 min,初代培养中,顶芽的培养效果比带腋芽茎段更好;以顶芽为外植体,初代培养的最适培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,其平均有效芽诱导数为5.87;在增殖培养中,以带腋芽茎段诱导丛生芽,其最适增殖培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA,增殖系数为6.92;以叶片切段诱导不定芽,其最适增殖培养基为:MS+1.0 mg/L 6-BA+0.3 mg/L NAA,增殖系数为3.59;以叶柄诱导不定芽,其最适增殖培养基为:MS+1.5 mg/L 6-BA+0.15 mg/L NAA,增殖系数为0.49;最适生根培养基为:1/2MS+0.35 mg/L IBA,生根率100%。本研究结果可以为日本独头白菊‘精诚’的工厂化生产提供技术支持,具有重要的实践价值。
In vitro rapid propagation of Japanese single-headed white chrysanthemum ‘Jingcheng' was made by using apical buds and stem segments with axillary buds as explants. In primary culture, explants were best sterilized with 0.1% mercuric chloride as surface disinfectant for 5 minutes, and the apical buds had better culture results than the axillary buds. The optimal medium for culture of the apical buds was MS+ 0.5 mg/L 6-BA and 0.1 mg/L NAA, which induced 5.87 effective shoots by average. In proliferation culture, the shoots from the primary culture were cut into stem segments with axillary buds and induced to generate clustered shoots on the optimal medium of MS+1.0 mg/L of 6-BA+0.3 mg/L of NAA,and its multiplication coefficient was 6.92. The optimal medium for inducing adventitious shoots from the leaf was MS+1.0 mg/L of 6-BA+0.3 mg/L of NAA, and the multiplication coefficient of 3.59. The optimal proliferation medium for inducing adventitious shoots from the petioles of the leaves was MS +1.5 mg/L 6-BA+0.15 mg/L NAA, and the multiplication coefficient was only 0.49. The optimum medium for root induction was 1/2 MS+0.35 mg/L IBA and the root inducing rate was upto 100%. These results will provide technical support for commercial production of elite seedlings of Japanese single-headed white chrysanthemum ‘Jingcheng', and has important practical value.
In vitro rapid propagation of Japanese single-headed white chrysanthemum ‘Jingcheng' was made by using apical buds and stem segments with axillary buds as explants. In primary culture, explants were best sterilized with 0.1% mercuric chloride as surface disinfectant for 5 minutes, and the apical buds had better culture results than the axillary buds. The optimal medium for culture of the apical buds was MS+ 0.5 mg/L 6-BA and 0.1 mg/L NAA, which induced 5.87 effective shoots by average. In proliferation culture, the shoots from the primary culture were cut into stem segments with axillary buds and induced to generate clustered shoots on the optimal medium of MS+1.0 mg/L of 6-BA+0.3 mg/L of NAA,and its multiplication coefficient was 6.92. The optimal medium for inducing adventitious shoots from the leaf was MS+1.0 mg/L of 6-BA+0.3 mg/L of NAA, and the multiplication coefficient of 3.59. The optimal proliferation medium for inducing adventitious shoots from the petioles of the leaves was MS +1.5 mg/L 6-BA+0.15 mg/L NAA, and the multiplication coefficient was only 0.49. The optimum medium for root induction was 1/2 MS+0.35 mg/L IBA and the root inducing rate was upto 100%. These results will provide technical support for commercial production of elite seedlings of Japanese single-headed white chrysanthemum ‘Jingcheng', and has important practical value.
引文
[1]Oberfield S E,Witchel S.“In vitro”multiplication of chrysanthemum morifolium ramat[J].Hormone Research in Paediatrics,2005,51(2):141-147.
[2]陈芝华.非洲菊组培苗湿润育苗技术与成本控制研究[D].南京:南京农业大学,2014.
[3]周婷.出口切花菊‘白扇’的引种调查与快繁技术的研究[D].福州:福建农林大学,2016.
[4]张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014(12):19-22.
[5]张月娇.菊花“神马”组织培养快繁技术研究[J].林业勘察设计.2012(1):139-142.
[6]邱美欢,曲军远,覃和业,等.“神马”菊组织培养技术研究[J].热带农业科学,2008,28(1):30-31.
[7]张燕,王志勇.菊花组织培养技术研究进展[J].廊坊师范学院学报(自然科学版),2015,15(4):80-85.
[8]王自布,莫国秀,罗会兰,等.菊花不同外植体组培快繁及其再生体系的研究[J].北方园艺,2015(18):106-109.
[9]袁成志,李波,杨蔚然.菊花组织培养技术研究[J].北方园艺,2010(16):154-156.
[10]肖志坚,纪艳,刘德江,等.菊花的组织培养技术[J].园艺与种苗,2012(3):21-23.
[11]孙婵娟.万寿菊的快速繁殖及试管开花研究[D].重庆:西南大学,2009.
[12]钟海丰,余锋锋,扬毅,等.菊花茎尖克隆体外繁殖研究[J].安徽农业科学,2011,39(29):17 811-17 813.
[13]Karim Mz A M A Z,Oberfield S E.Rapid multiplication of Chrysanthemum morifolium through in vitro culture[J].2003.
[14]张家瑛.贡菊离体快繁体系优化及种苗分级标准研究[D].广州:广州中医药大学,2016.
[15]陈兵先,黄宝灵,吕成群,等.植物组织培养试管苗玻璃化现象研究进展[J].林业科技开发,2011,25(1):1-5.
[16]蔡祖国,徐小彪,周会萍.植物组织培养中的玻璃化现象及其预防[J].生物技术通讯,2005,16(3):353-355.
[17]徐清燏.5个重要菊花品种高效不定芽再生体系的研究[D].北京:北京林业大学,2005.
[18]蒋细旺,刘国锋,包满珠.菊花9个品种叶片和茎段快速高效再生体系的建立[J].华中农业大学学报.2003(2):162-166.
[19]张艳利.金盏菊叶柄组织培养技术初探[J].现代农业.2016(2):95-96.
[20]陈雪鹃,吴珏,李雪珂,等.芙蓉菊组培快繁技术的研究[J].中南林业科技大学学报,2012,32(7):100-104,127,封3.
[21]李露华.贡菊种苗离体快繁与复壮技术的研究[D].广州:广州中医药大学,2015.
[22]王丽华.银胶菊组培技术及玻璃化防止措施研究[D].雅安:四川农业大学,2005.
[23]肖秀丽.出口专用型切花菊离体快繁及生产应用技术研究[D].泰安:山东农业大学,2005.
[24]Waseem K,Jilani M S,Khan M S.Rapid plant regeneration of chrysanthemum(Chrysanthemum morifolium I.)through shoot tip culture[J].African Journal of Biotechnology,2009,8(9):1 871-1 877.
[2]陈芝华.非洲菊组培苗湿润育苗技术与成本控制研究[D].南京:南京农业大学,2014.
[3]周婷.出口切花菊‘白扇’的引种调查与快繁技术的研究[D].福州:福建农林大学,2016.
[4]张天静,雷家军,孙文松.菊花品种神马的茎段离体快繁研究[J].黑龙江农业科学,2014(12):19-22.
[5]张月娇.菊花“神马”组织培养快繁技术研究[J].林业勘察设计.2012(1):139-142.
[6]邱美欢,曲军远,覃和业,等.“神马”菊组织培养技术研究[J].热带农业科学,2008,28(1):30-31.
[7]张燕,王志勇.菊花组织培养技术研究进展[J].廊坊师范学院学报(自然科学版),2015,15(4):80-85.
[8]王自布,莫国秀,罗会兰,等.菊花不同外植体组培快繁及其再生体系的研究[J].北方园艺,2015(18):106-109.
[9]袁成志,李波,杨蔚然.菊花组织培养技术研究[J].北方园艺,2010(16):154-156.
[10]肖志坚,纪艳,刘德江,等.菊花的组织培养技术[J].园艺与种苗,2012(3):21-23.
[11]孙婵娟.万寿菊的快速繁殖及试管开花研究[D].重庆:西南大学,2009.
[12]钟海丰,余锋锋,扬毅,等.菊花茎尖克隆体外繁殖研究[J].安徽农业科学,2011,39(29):17 811-17 813.
[13]Karim Mz A M A Z,Oberfield S E.Rapid multiplication of Chrysanthemum morifolium through in vitro culture[J].2003.
[14]张家瑛.贡菊离体快繁体系优化及种苗分级标准研究[D].广州:广州中医药大学,2016.
[15]陈兵先,黄宝灵,吕成群,等.植物组织培养试管苗玻璃化现象研究进展[J].林业科技开发,2011,25(1):1-5.
[16]蔡祖国,徐小彪,周会萍.植物组织培养中的玻璃化现象及其预防[J].生物技术通讯,2005,16(3):353-355.
[17]徐清燏.5个重要菊花品种高效不定芽再生体系的研究[D].北京:北京林业大学,2005.
[18]蒋细旺,刘国锋,包满珠.菊花9个品种叶片和茎段快速高效再生体系的建立[J].华中农业大学学报.2003(2):162-166.
[19]张艳利.金盏菊叶柄组织培养技术初探[J].现代农业.2016(2):95-96.
[20]陈雪鹃,吴珏,李雪珂,等.芙蓉菊组培快繁技术的研究[J].中南林业科技大学学报,2012,32(7):100-104,127,封3.
[21]李露华.贡菊种苗离体快繁与复壮技术的研究[D].广州:广州中医药大学,2015.
[22]王丽华.银胶菊组培技术及玻璃化防止措施研究[D].雅安:四川农业大学,2005.
[23]肖秀丽.出口专用型切花菊离体快繁及生产应用技术研究[D].泰安:山东农业大学,2005.
[24]Waseem K,Jilani M S,Khan M S.Rapid plant regeneration of chrysanthemum(Chrysanthemum morifolium I.)through shoot tip culture[J].African Journal of Biotechnology,2009,8(9):1 871-1 877.