陕西产黄精产地加工与炮制一体化工艺质量研究
|
摘要
目的:对不同炮制方法的黄精饮片质量相关指标进行分析,为今后黄精的加工炮制工作提出可供参考的根据。方法:采用硅胶G薄层板对不同炮制工艺的黄精饮片进行定性鉴别;对不同炮制工艺的黄精饮片浸出物、灰分、水分以及多糖含量进行测定;采用甲状腺素片诱导小鼠阴虚模型并评价不同黄精饮片滋阴效果。结果:黄精不同炮制品在薄层色谱系统中的行为特征和薯蓣皂苷元对照品的在色谱系统中的行为特征一致,检查项和含量测定结果均符合《中国药典》(2015年版)和《陕西省中药饮片标准》相关规定;酒蒸后黄精滋阴作用有所增强,且以先切的酒黄精饮片强于先蒸的酒黄精饮片。结论:不同炮制工艺的黄精饮片在质量上存在着一定的差异,为后续黄精产地加工与炮制一体化工艺研究提供借鉴。
Objective: To compare quality and nourishing yin effect of Polygonatum sibiricum pieces between three integrated production processed. Methods: The silica gel G thin plate was used to evaluation the qualitative identification of different processing technology. The extract, ash, moisture and polysaccharide content were used to evaluation the quantitative identification of different processing technology. The Yin deficiency model rats induced by thyroxine was used tablets was used to evaluation ourishing yin effect of different processing technology. Results: The behavior characteristics of different processed products of Polygonatum sibiricum in TLC and the behavior characteristics of diosgenin control were consistent in the chromatographic system. The test items and content determination results were in accordance with the 2015 edition of the Chinese Pharmacopoeia and the standard of Chinese herbal medicine in Shaanxi province. And th nourishing yin effect of first cut and steamed of polygonati rhizoma with wine was better than first steamed and cut. Conclusion: Different processing methods of Polygonatum sibiricum have certain difference in quality, and our results provide a reference for the study on p habitat processing and pieces processing of Polygonatum sibiricum from Shaanxi.
Objective: To compare quality and nourishing yin effect of Polygonatum sibiricum pieces between three integrated production processed. Methods: The silica gel G thin plate was used to evaluation the qualitative identification of different processing technology. The extract, ash, moisture and polysaccharide content were used to evaluation the quantitative identification of different processing technology. The Yin deficiency model rats induced by thyroxine was used tablets was used to evaluation ourishing yin effect of different processing technology. Results: The behavior characteristics of different processed products of Polygonatum sibiricum in TLC and the behavior characteristics of diosgenin control were consistent in the chromatographic system. The test items and content determination results were in accordance with the 2015 edition of the Chinese Pharmacopoeia and the standard of Chinese herbal medicine in Shaanxi province. And th nourishing yin effect of first cut and steamed of polygonati rhizoma with wine was better than first steamed and cut. Conclusion: Different processing methods of Polygonatum sibiricum have certain difference in quality, and our results provide a reference for the study on p habitat processing and pieces processing of Polygonatum sibiricum from Shaanxi.
引文
[1]国家药典委员会.中国药典(一部)[S].北京:中国医药科技出版社,2015:306-307.
[2]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,2004.
[3]杨华杰,龚千锋.黄精炮制研究的进展[J].中国实验方剂学杂志,2017,23(3):216-217.
[4]何俊婷,曹爱兰,赵新礼,等.不同基原与产地的黄精对比研究[J].中国药业,2017,26(19):23-26.
[5]张洁,马百平,杨云,等.黄精属植物甾体皂苷类成分及药理活性研究进展[J].中国药学杂志,2006,41(5):330-332.
[6]杨明河,于德泉.黄精多糖和低聚糖的研究[J].药学通报,1980,15(7):44-44.
[7]余红,张小平,邓明强,等.多花黄精挥发油GC-MS分析及其生物活性研究[J].中国实验方剂学杂志,2008,14(5):4-5.
[8]孙隆儒,王素贤,李铣.中药黄精中的新生物碱[J].中国药物化学杂志,1997,7(2):129.
[8]付婷婷,王国贤,陈婷婷,等.黄精多糖对糖尿病肾病大鼠肾脏的保护作用[J].中药药理与临床,2015,31(4):124-125.
[10]段华,王保奇,张跃文.黄精多糖对肝癌H22移植瘤小鼠的抑瘤作用及机制研究[J].中药新药与临床药理,2014,25(1):5-7.
[11]郑春艳,汪好芬,张庭廷.黄精多糖的抑菌和抗炎作用研究[J].安徽师范大学学报(自然科学版),2010,33(3):272-274.
[12]陈辉,冯珊珊,孙彦君,等.3种药用黄精的化学成分及药理活性研究进展[J].中草药,2015,46(15):2335-2336.
[13]刘建军,刘玲,夏浇,等.黄精及其炮制品的薄层鉴别研究[J].中国民族民间医药,2015,3(5):14-16.
[14]吴良发,宁火华,岳翃熠,等.正交设计研究黄精炮制中美拉德反应产物的抗氧化活性[J].中国药师,2015,18(6):916-919.
[15]祁岩龙,冯怀章,于洋,等.美拉德反应研究进展及在食品工业中的应用[J].食品工业,2018,39(3):248-252.
[16]贺玉伟,柴程芝,寇俊萍,等.甲状腺素诱导小鼠模型表观指征变化与阴虚火旺证的相关性研究[J].实验动物学,2013,30(2):1-7.
[17]吴柳花,吕圭源,李波,等.黄精对长期超负荷游泳致阴虚内热模型大鼠的作用研究[J].中国中药杂志,2014,39(10):1886-1891.
[18]雷珊珊,吕圭源,金泽武,等.铁皮石斛花提取物对甲亢型阴虚小鼠的影响[J].中国中药杂志,2015,40(9):1793-1798.
[19]付晓伶,方肇勤.阴虚证动物模型的造模方法及评析[J].上海中医药大学学报,2004,18(2):51-54.
[20]李威,杨宁辉,崔英,等.地黄不同炮制品对阴虚模型小鼠血浆cAMP、cGMP影响的实验研究[J].中国卫生检验杂志,2009,19(9):1982-1983.
[2]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,2004.
[3]杨华杰,龚千锋.黄精炮制研究的进展[J].中国实验方剂学杂志,2017,23(3):216-217.
[4]何俊婷,曹爱兰,赵新礼,等.不同基原与产地的黄精对比研究[J].中国药业,2017,26(19):23-26.
[5]张洁,马百平,杨云,等.黄精属植物甾体皂苷类成分及药理活性研究进展[J].中国药学杂志,2006,41(5):330-332.
[6]杨明河,于德泉.黄精多糖和低聚糖的研究[J].药学通报,1980,15(7):44-44.
[7]余红,张小平,邓明强,等.多花黄精挥发油GC-MS分析及其生物活性研究[J].中国实验方剂学杂志,2008,14(5):4-5.
[8]孙隆儒,王素贤,李铣.中药黄精中的新生物碱[J].中国药物化学杂志,1997,7(2):129.
[8]付婷婷,王国贤,陈婷婷,等.黄精多糖对糖尿病肾病大鼠肾脏的保护作用[J].中药药理与临床,2015,31(4):124-125.
[10]段华,王保奇,张跃文.黄精多糖对肝癌H22移植瘤小鼠的抑瘤作用及机制研究[J].中药新药与临床药理,2014,25(1):5-7.
[11]郑春艳,汪好芬,张庭廷.黄精多糖的抑菌和抗炎作用研究[J].安徽师范大学学报(自然科学版),2010,33(3):272-274.
[12]陈辉,冯珊珊,孙彦君,等.3种药用黄精的化学成分及药理活性研究进展[J].中草药,2015,46(15):2335-2336.
[13]刘建军,刘玲,夏浇,等.黄精及其炮制品的薄层鉴别研究[J].中国民族民间医药,2015,3(5):14-16.
[14]吴良发,宁火华,岳翃熠,等.正交设计研究黄精炮制中美拉德反应产物的抗氧化活性[J].中国药师,2015,18(6):916-919.
[15]祁岩龙,冯怀章,于洋,等.美拉德反应研究进展及在食品工业中的应用[J].食品工业,2018,39(3):248-252.
[16]贺玉伟,柴程芝,寇俊萍,等.甲状腺素诱导小鼠模型表观指征变化与阴虚火旺证的相关性研究[J].实验动物学,2013,30(2):1-7.
[17]吴柳花,吕圭源,李波,等.黄精对长期超负荷游泳致阴虚内热模型大鼠的作用研究[J].中国中药杂志,2014,39(10):1886-1891.
[18]雷珊珊,吕圭源,金泽武,等.铁皮石斛花提取物对甲亢型阴虚小鼠的影响[J].中国中药杂志,2015,40(9):1793-1798.
[19]付晓伶,方肇勤.阴虚证动物模型的造模方法及评析[J].上海中医药大学学报,2004,18(2):51-54.
[20]李威,杨宁辉,崔英,等.地黄不同炮制品对阴虚模型小鼠血浆cAMP、cGMP影响的实验研究[J].中国卫生检验杂志,2009,19(9):1982-1983.