Ca(OH)_2固态温和预处理玉米秸秆的条件优化及对产气的影响
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摘要
为了提高玉米秸秆的比表面积、增加溶出物中还原糖的含量,使其更容易被微生物降解利用,基于Ca(OH)_2固态温和预处理的条件,利用中心组合实验设计方法对处理条件进行优化;在优化条件的基础上,选择NaOH与Ca(OH)_2进行组合,考察组合碱配比对秸秆预处理效果及产气的影响。结果表明,最佳Ca(OH)_2处理条件为:60℃、Ca(OH)_2投加量10%、处理48h,溶出物中还原糖含量最大,为234.81mg·L-1;最佳组合碱处理条件为:60℃、碱投加量8%[NaOH与Ca(OH)_2的质量比为75∶25]、处理48h,溶出物中还原糖含量为244.17 mg·L-1、累积产气量为3 701mL、单位产气量为289.25mL·(g VS)-1、纤维素和半纤维素的降解率分别为33.69%和3.21%。
In order to increase the specific surface area of corn stover and the content of reducing sugar in leachate so that the corn stover is more easily utilized by microorganisms,we optimized the treatment conditions by a central combination experimental design method on the basis of Ca(OH)_2 solid-state mild pretreatment conditions.Combinated with NaOH and Ca(OH)_2,we investigated the influences of the mass ratio of combined alkali on pretreatment effect of corn stover and gas production.The results showed that the content of reducing sugar in leachate reached the highest of 234.81 mg·L-1 when the alkali addition was 10% and the treatment time was 48 hat 60℃.The optimum pretreatment conditions of combined alkali were as follows:60℃,the alkali addition of 8%(the mass ratio of NaOH and Ca(OH)_2 was 75∶25),and the treatment time of 48 h.Under above conditions,the content of reducing sugar in leachate was 244.17 mg·L-1,the cumulative biogas production was 3 701 mL,the unit biogas production was 289.25 mL·(g VS)-1,and the degradation rates of cellulose and hemicellulose were 33.69% and 3.21%,respectively.
In order to increase the specific surface area of corn stover and the content of reducing sugar in leachate so that the corn stover is more easily utilized by microorganisms,we optimized the treatment conditions by a central combination experimental design method on the basis of Ca(OH)_2 solid-state mild pretreatment conditions.Combinated with NaOH and Ca(OH)_2,we investigated the influences of the mass ratio of combined alkali on pretreatment effect of corn stover and gas production.The results showed that the content of reducing sugar in leachate reached the highest of 234.81 mg·L-1 when the alkali addition was 10% and the treatment time was 48 hat 60℃.The optimum pretreatment conditions of combined alkali were as follows:60℃,the alkali addition of 8%(the mass ratio of NaOH and Ca(OH)_2 was 75∶25),and the treatment time of 48 h.Under above conditions,the content of reducing sugar in leachate was 244.17 mg·L-1,the cumulative biogas production was 3 701 mL,the unit biogas production was 289.25 mL·(g VS)-1,and the degradation rates of cellulose and hemicellulose were 33.69% and 3.21%,respectively.
引文
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[23]杨茜.可渗透固定化硫酸盐还原菌反应墙的碳源缓释规律与可利用性研究[D].合肥:安徽工程大学,2014.YANG Q.Permeable immobilized sulfate-reducing bacteria reactive barrier of carbon source slow-release laws and availability of research[D].Hefei:Anhui Polytechnic University,2014.
[24]李建,刘庆玉,郎咸明,等.响应面法优化沼液预处理玉米秸秆条件的研究[J].可再生能源,2016,34(2):292-297.LI J,LIU Q Y,LANG X M,et al.Process parameters optimization of maize straw pretreated by biogas slurry using response surface methodology[J].Renewable Energy Resources,2016,34(2):292-297.
[25]李芳.混合原料厌氧发酵工艺的响应面优化研究[D].杨凌:西北农林科技大学,2013.LI F.The optimization of fermentation process of manure and straw by response surface methodology[D].Yangling:Northwest A&F University,2013.
[2]杨茜,鞠美庭,李维尊.秸秆厌氧消化产甲烷的研究进展[J].农业工程学报,2016,32(14):232-242.YANG Q,JU M T,LI W Z.Review of methane production from straws anaerobic digestion[J].Transactions of the Chinese Society of Agricultural Engineering,2016,31(14):232-242.
[3]ZHENG Y,ZHAO J,XU F Q,et al.Pretreatment of lignocellulosic biomass for enhanced biogas production[J].Progress in Energy and Combustion Science,2014,42:35-53.
[4]FAYYAZ A S,QAISAR M,NAIM R,et al.Co-digestion,pretreatment and digester design for enhanced methanogenesis[J].Renewable and Sustainable Energy Reviews,2015,42:627-642.
[5]LI J H,ZHANG R H,MUHAMMAD A H S,et al.Enhancing methane production of corn stover through a novel way:sequent pretreatment of potassium hydroxide and steam explosion[J].Bioresource Technology,2015,181:345-350.
[6]YE C,JAY J C,KURT S C.Inhibition of anaerobic digestion process:a review[J].Bioresource Technology,2008,99:4044-4064.
[7]黑里贝特·因萨姆,英格丽德·弗兰克-怀特,玛塔·戈韦尔纳,编.鞠美庭,王平,黄访,等译.微生物的作用——从废物到资源[M].北京:化学工业出版社,2012.INSAM H,FRANK-WHITTLEI Y,COBERNA M,Edition.JU M T,WANG P,HUANG F,Translation.Microbes at Work—From Wastes to Resources[M].Beijing:Chemistry Industry Press,2012.
[8]宋籽霖.秸秆沼气厌氧发酵的预处理工艺优化及经济实用性分析[D].杨凌:西北农林科技大学,2013.SONG Z L.Optimization of straw pretreatment for anaerobic fermentation and analysis of economic practicability[D].Yangling:Northwest A&F University,2013.
[9]王建,赵玲,田萌萌,等.组合碱预处理对玉米秸秆厌氧消化的影响[J].太阳能学报,2014,35(12):2577-2581.WANG J,ZHAO L,TIAN M M,et al.Influence of combined alkali pretreatment on anaerobic digestion of corn stalk[J].Acta Energiae Solaris Sinica,2014,35(12):2577-2581.
[10]石贤礼,何宁,邓旭.碱-组合工艺预处理剩余污泥研究进展[J].生物技术,2012,22(6):89-93.SHI X L,HE N,DENG X.Progress on pretreatment of excess sludge by alkali-combined processes[J].Biotechnology,2012,22(6):89-93.
[11]LI L,CHEN C,ZHANG R H,et al.Pretreatment of corn stover for methane production with the combination of potassium hydroxide and calcium hydroxide[J].Energy&Fuels,2015,29:5841-5846.
[12]PECES M,ASTALS S,MATA-ALVAREZ J.Effect of moisture on pretreatment efficiency for anaerobic digestion of lignocellulose substrates[J].Waste Management,2015,46:189-196.
[13]ZHENG M X,LI X J,LI L Q,et al.Enhancing anaerobic biogasification of corn stover through wet state NaOH pretreatment[J].Bioresource Technology,2009,100:5140-5145.
[14]GIROLAMO G D,GRIGATTI M,BERTIN L,et al.Enhanced substrate degradation and methane yield with maleic acid pretreatments in biomass crops and residues[J].Biomass and Bioenergy,2016,85:306-312.
[15]PASSOS F,GARCA J,FERRER I.Impact of low temperature pretreatment on the anaerobic digestion of microalgal biomass[J].Bioresource Technology,2013,138(2):79-86.
[16]YUAN H R,LI R P,ZHANG Y T,et al.Anaerobic digestion of ammonia-pretreated corn stover[J].Biosystems Engineering,2015,129:142-148.
[17]YOU Z,WEI T Y,CHENG J J.Improving anaerobic codigestion of corn stover using sodium hydroxide pretreatment[J].Energy&Fuels,2014,28:549-554.
[18]MANSIKKAMAKI P,LAHTINEN M,RISSANEN K.Structural changes of cellulose crystallites induced by mercerisation in different solvent systems;determined by powder X-ray diffraction method[J].Cellulose,2005,12:233-242.
[19]孙付保,王亮,谭玲,等.木质纤维素糖平台基质组成结构的分析表征技术研究进展[J].化工进展,2014,33(4):883-895.SUN F B,WANG L,TAN L,et al.Analytic technology of characterizing the substrate architecture in the lignocellulose-to-sugar platform[J].Chemical Industry and Engineering Progress,2014,33(4):883-895.
[20]ZHU Y M,LEE Y Y,BLANDER R T.Optimization of diluteacid pretreatment of corn stover using a high-solids percolation reactor[J].Applied Biochemisty&Biotechnology,2005,121-124:1045-1054.
[21]APHA,AWWA&WEF.Standard Methods for the Examination of Water and Wastewater,19th edition.American Public Health Association/America Water Works Association/Water Environment Federation[M].Washington DC,USA,1995.
[22]杨秋林.农业废弃物固体碱预处理过程中木素的结构表征及其脱除机理研究[D].广州:华南理工大学,2013.YANG Q L.The structural characterization of lignin in process of pretreatment of agricultural residues by solid alkali and study on delignification mechanism[D].Guangzhou:South China University of Technology,2013.
[23]杨茜.可渗透固定化硫酸盐还原菌反应墙的碳源缓释规律与可利用性研究[D].合肥:安徽工程大学,2014.YANG Q.Permeable immobilized sulfate-reducing bacteria reactive barrier of carbon source slow-release laws and availability of research[D].Hefei:Anhui Polytechnic University,2014.
[24]李建,刘庆玉,郎咸明,等.响应面法优化沼液预处理玉米秸秆条件的研究[J].可再生能源,2016,34(2):292-297.LI J,LIU Q Y,LANG X M,et al.Process parameters optimization of maize straw pretreated by biogas slurry using response surface methodology[J].Renewable Energy Resources,2016,34(2):292-297.
[25]李芳.混合原料厌氧发酵工艺的响应面优化研究[D].杨凌:西北农林科技大学,2013.LI F.The optimization of fermentation process of manure and straw by response surface methodology[D].Yangling:Northwest A&F University,2013.