茯砖茶中茶多酚的纯化工艺研究
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摘要
本文选用茯砖茶为原料,通过超声波辅助水浴浸提法得到茶多酚提取液,所得提取液分别用离子沉淀法和大孔树脂吸附法纯化﹒离子沉淀法纯化茶多酚的最佳条件:AlCl_3为沉淀剂,m_(沉淀剂)︰m_(茶多酚)=1︰0.04,NaHCO_3为pH调节剂,调pH=6.0,0.5mol/L盐酸溶解沉淀,茶多酚粗得率为89.99%﹒大孔树脂吸附法纯化茶多酚的最佳条件:吸附时间为40 min,m_(大孔树脂)︰m_(茶多酚)=5︰3,洗脱时间为40 min,茶多酚粗得率为30.98%﹒
In this paper the tea polyphenols were extracted from Fuzhuan tea by ultrasonic-assisted water bath extraction. The extracted liquid was purified by ion precipitation and macroporous resin adsorptionrespectively. The optimum condition for purification of the tea polyphenols by ion precipitation method wasthat the precipitant is AlCl_3, the mass ratio of precipitant to tea polyphenols is 1︰0.04, the pH regulator isNaHCO_3, the optimum pH of the environment is 6, the concentration of the dissolved hydrochloric acid is 0.5mol/L, and the yield of the extracted tea polyphenols is 89.99%. The best condition for the purification of teapolyphenols by macroporous resin adsorption method was that the adsorption time is 40 min, the mass ratio of macroporous resin to tea polyphenols is 5︰3, the elution of 40 min, the yield of tea polyphenols is 30.98%.
In this paper the tea polyphenols were extracted from Fuzhuan tea by ultrasonic-assisted water bath extraction. The extracted liquid was purified by ion precipitation and macroporous resin adsorptionrespectively. The optimum condition for purification of the tea polyphenols by ion precipitation method wasthat the precipitant is AlCl_3, the mass ratio of precipitant to tea polyphenols is 1︰0.04, the pH regulator isNaHCO_3, the optimum pH of the environment is 6, the concentration of the dissolved hydrochloric acid is 0.5mol/L, and the yield of the extracted tea polyphenols is 89.99%. The best condition for the purification of teapolyphenols by macroporous resin adsorption method was that the adsorption time is 40 min, the mass ratio of macroporous resin to tea polyphenols is 5︰3, the elution of 40 min, the yield of tea polyphenols is 30.98%.
引文
[1]李睿,帅建军.茶多酚的分离提取及其性质研究[J].江苏教育学院学报:自然科学版, 2008, 25(4):26-29.
[2]居华.茶多酚生产应用与开发前景[J].化工中间体,2003(15):26-29.
[3]潘卫仓.茶多酚的功效及提取工艺研究进展[J].甘肃科技,2011, 27(15):57-58.
[4]杨立杰.乌龙茶中茶多酚、茶多糖的提取及其纯化和性质研究[D].厦门:集美大学, 2007.
[5]郑明珠,迟玉杰.天然抗氧化剂-茶多酚[J].食品研究与开发,2003, 24(6):108-111.
[6]刘家红.茶叶有效成分提取分离技术研究进展[J].南方农业,2010, 4(5):99-101.
[7]李南薇,詹金广,陈少航.茶多酚分离提取和应用研究进展[J].天津农业科学, 2010, 16(4):8-10, 14.
[8]黄丽凤,刘友平,黎代余.茶多酚提取纯化工艺研究进展[J].中国食品添加剂, 2010(1):69-72.
[9]王斌,江和源,张建勇,等.酶工程技术在茶叶深加工中的应用及展望[J].茶叶科学, 2010, 30(增1):521-526.
[10]阮玉凤,黄祉健,朱晓莹,等.植物多酚提取方法的研究进展[J].广东化工, 2013, 40(21):89-90.
[11]宋莎莎,李媛,刘石泉,等.茯砖茶中茶多酚的提取工艺研究[J].湖南城市学院学报:自然科学版, 2017, 26(5):70-73.
[2]居华.茶多酚生产应用与开发前景[J].化工中间体,2003(15):26-29.
[3]潘卫仓.茶多酚的功效及提取工艺研究进展[J].甘肃科技,2011, 27(15):57-58.
[4]杨立杰.乌龙茶中茶多酚、茶多糖的提取及其纯化和性质研究[D].厦门:集美大学, 2007.
[5]郑明珠,迟玉杰.天然抗氧化剂-茶多酚[J].食品研究与开发,2003, 24(6):108-111.
[6]刘家红.茶叶有效成分提取分离技术研究进展[J].南方农业,2010, 4(5):99-101.
[7]李南薇,詹金广,陈少航.茶多酚分离提取和应用研究进展[J].天津农业科学, 2010, 16(4):8-10, 14.
[8]黄丽凤,刘友平,黎代余.茶多酚提取纯化工艺研究进展[J].中国食品添加剂, 2010(1):69-72.
[9]王斌,江和源,张建勇,等.酶工程技术在茶叶深加工中的应用及展望[J].茶叶科学, 2010, 30(增1):521-526.
[10]阮玉凤,黄祉健,朱晓莹,等.植物多酚提取方法的研究进展[J].广东化工, 2013, 40(21):89-90.
[11]宋莎莎,李媛,刘石泉,等.茯砖茶中茶多酚的提取工艺研究[J].湖南城市学院学报:自然科学版, 2017, 26(5):70-73.