慢性疲劳综合征大鼠模型相关指标观测
|
摘要
目的采用拥挤等复合应激制作慢性疲劳综合征(CFS)大鼠模型,并观测相关指标。方法综合运用不定期的拥挤、噪音和昼夜颠倒等不同的应激因子长期温和刺激大鼠,造模持续64 d。观察大鼠精神状态、皮毛色泽,每周测体质量,每月进行半定量评分和摄食量测定,采血检测血常规、免疫球蛋白G(Ig G)及血乳酸的含量,试验期末强迫游泳测定不动时间。结果与正常对照组比较,造模大鼠自主活动减少、精神萎靡、皮肤松弛、毛发变黄、耳尾颜色淡红少泽,半定量评分值升高;体质量、摄食量下降;白细胞、红细胞、血红蛋白、红细胞比容等下降;强迫游泳后不动时间、血乳酸和Ig G上升。结论采用拥挤、噪音和睡眠紊乱成功构建CFS大鼠模型,半定量评分、大鼠体质量、摄食量、白细胞、红细胞、血红蛋白、红细胞比容、强迫游泳后不动时间、血乳酸和Ig G可以作为参考指标。
Objective To make a rat model of chronic fatigue syndrome(CFS) by overcrowding and other stresses, and to test the related indicators. Methods The rats were stimulated by different stress factors, such as irregular crowding, noise and diurnal reversal for 64 days. The mental status, coat and skin color were observed. Weight and food intake were measured every week. Blood routine, immunoglobulin G(Ig G) and blood lactate were measured regularly. Results After modeling, rat independent activity decreased. Mental fatigue, skin loosed, body hair withered, and semi-quantitative score value increased. Body weight and food intake decreased. White blood cell, red blood cell, hemoglobin and erythrocyte specific volume decreased. Inactive time after forced swimming, blood lactic acid and Ig G increased. Conclusion The rat models of CFS were successfully constructed by overcrowding, noise and sleep disorders. Semi-quantitative scores, body weight, food intake, white blood cell, red blood cell,hemoglobin, erythrocyte specific volume, inactive time after forced swimming, blood lactic acid and Ig G can be used as reference indicators.
Objective To make a rat model of chronic fatigue syndrome(CFS) by overcrowding and other stresses, and to test the related indicators. Methods The rats were stimulated by different stress factors, such as irregular crowding, noise and diurnal reversal for 64 days. The mental status, coat and skin color were observed. Weight and food intake were measured every week. Blood routine, immunoglobulin G(Ig G) and blood lactate were measured regularly. Results After modeling, rat independent activity decreased. Mental fatigue, skin loosed, body hair withered, and semi-quantitative score value increased. Body weight and food intake decreased. White blood cell, red blood cell, hemoglobin and erythrocyte specific volume decreased. Inactive time after forced swimming, blood lactic acid and Ig G increased. Conclusion The rat models of CFS were successfully constructed by overcrowding, noise and sleep disorders. Semi-quantitative scores, body weight, food intake, white blood cell, red blood cell,hemoglobin, erythrocyte specific volume, inactive time after forced swimming, blood lactic acid and Ig G can be used as reference indicators.
引文
[1]曹东萍,谭福彬,刘文成,等.亚健康问题研究现状及进展[J].中华疾病控制杂志,2009,13(1):91-93.
[2]Alfredo AF,Alvaro PM,Maravillas IM,et al.Chronic fatigue syndrome:aetiology,diagnosis and treatmen[J].BMC Psychiatry,2009,9(Suppl 1):S1.
[3]饶子亮,王诺,郑佳琳,等.慢性疲劳综合症大鼠模型的建立[J].实验动物科学,2014,31(4):24-27.
[4]张北月,石晋丽,郑志全,等.DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用[J].中国实验动物学报,2017,25(1):85-89.
[5]唐迪,张德安.医护人员慢性疲劳综合征前瞻记忆的研究[J].中国疗养医学,2018,27(2):131-132.
[6]林红,汪小清,胡轩,等.珠海市医务人员慢性疲劳状况与心理健康的相关性分析[J].工业卫生与职业病,2017,43(2):140-148.
[7]Johnston S,Brenu EW,Staines D,et al.The prevalence of chronic fatigue syndrome/myalgic encephalomyelitis:a metaanalysis[J].Clin Epidemiol,2013,5:105-110.
[8]陈奇,严恺,景灵君,等.公务员慢性疲劳综合征现况及影响因素分析[J].中国公共卫生,2016,32(5):664-666.
[9]魏红梅,胡松,王燕,等.青岛市黄岛区高校教师慢性疲劳综合征现状调查及影响因素分析[J].青岛大学医学院学报第,2016,52(2):164-168.
[10]刘月园,吴磊,袁也丰,等.南昌市东湖区中小学教师慢性疲劳综合征患病现状及影响因素分析[J].中华疾病控制杂志,2014,18(7):643-645.
[11]赵红勤.大学生慢性疲劳综合征的体适能特征及运动对策[J].聊城大学学报:自然科学版,2015,28(4):25-28.
[12]Morris G,Berk M,Puri BK.A comparison of neuroimaging abnormalities in multiple sclerosis,major depression and chronic fatigue syndrome(myalgic ncephalomyelitis):is there a common cause[J].Mol Neurobiol,2018,55(4):3592-3609.
[13]李壮,刘洋,葛辛,等.基于数据挖掘的慢性疲劳综合征伴焦虑和抑郁状态的相关因素分析[J].中华全科医学,2018,16(2):204-206.
[14]王会杰.冠心病患者伴焦虑抑郁的原因分析及中西医护理干预措施[J].医学信息,2015,28(38):225.
[15]姜甜,贾友宏,李一石.高血压伴发焦虑和抑郁的研究进展[J].中国分子心脏病学杂志,2015,15(4):1424-1427.
[16]张丽,张振贤,黄瑶.不同慢性应激综合模式下慢性疲劳动物实验研究概况[J].中国中西医结合杂志,2016,36(8):1015-1020.
[17]Dong P,Zhao J,Li N,et al.Sevoflurane exaggerates cognitive decline in a rat model of chronic intermittent hypoxia by aggravating microglia-mediated neuroinflammation via downregulation of PPAR-γin the hippocampus[J].Behav Brain Res,2018,347:325-331.
[18]Cao Y,Li Q.The variation of the 5-hydroxytryptamine system between chronic unpredictable mild stress rats and chronic fatigue syndrome rats induced by forced treadmill running[J].Neuroreport,2017,28(11):630-637.
[19]Liu Z,Wu Y,Liu T,et al.Serotonin regulation in a rat model of exercise-induced chronic fatigue[J].Neuroscience,2017,349:27-34.
[20]Ishida J,Saitoh M,Doehner W,et al.Animal models of cachexia and sarcopenia in chronic illness:cardiac function,body composition changes and therapeutic results[J].Int J Cardiol,2017,238:12-18.
[21]古春青,王著敏,赵铎.红景天对复合应激因素致慢性疲劳大鼠体重及一般行为学的影响[J].中国处方药,2016,14(4):29-30.
[22]Farhan M,Ikram H,Kanwal S.Unpredictable chronic mild stress induced behavioral deficits:A comparative study in male and female rats[J].Pharm Sci,2014,27(4):879-884.
[23]余曦明,陈思源,余望贻.中医慢性疲劳综合症动物模型的建立与评价[J].基础医学,2014,4(2):22-24.
[2]Alfredo AF,Alvaro PM,Maravillas IM,et al.Chronic fatigue syndrome:aetiology,diagnosis and treatmen[J].BMC Psychiatry,2009,9(Suppl 1):S1.
[3]饶子亮,王诺,郑佳琳,等.慢性疲劳综合症大鼠模型的建立[J].实验动物科学,2014,31(4):24-27.
[4]张北月,石晋丽,郑志全,等.DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用[J].中国实验动物学报,2017,25(1):85-89.
[5]唐迪,张德安.医护人员慢性疲劳综合征前瞻记忆的研究[J].中国疗养医学,2018,27(2):131-132.
[6]林红,汪小清,胡轩,等.珠海市医务人员慢性疲劳状况与心理健康的相关性分析[J].工业卫生与职业病,2017,43(2):140-148.
[7]Johnston S,Brenu EW,Staines D,et al.The prevalence of chronic fatigue syndrome/myalgic encephalomyelitis:a metaanalysis[J].Clin Epidemiol,2013,5:105-110.
[8]陈奇,严恺,景灵君,等.公务员慢性疲劳综合征现况及影响因素分析[J].中国公共卫生,2016,32(5):664-666.
[9]魏红梅,胡松,王燕,等.青岛市黄岛区高校教师慢性疲劳综合征现状调查及影响因素分析[J].青岛大学医学院学报第,2016,52(2):164-168.
[10]刘月园,吴磊,袁也丰,等.南昌市东湖区中小学教师慢性疲劳综合征患病现状及影响因素分析[J].中华疾病控制杂志,2014,18(7):643-645.
[11]赵红勤.大学生慢性疲劳综合征的体适能特征及运动对策[J].聊城大学学报:自然科学版,2015,28(4):25-28.
[12]Morris G,Berk M,Puri BK.A comparison of neuroimaging abnormalities in multiple sclerosis,major depression and chronic fatigue syndrome(myalgic ncephalomyelitis):is there a common cause[J].Mol Neurobiol,2018,55(4):3592-3609.
[13]李壮,刘洋,葛辛,等.基于数据挖掘的慢性疲劳综合征伴焦虑和抑郁状态的相关因素分析[J].中华全科医学,2018,16(2):204-206.
[14]王会杰.冠心病患者伴焦虑抑郁的原因分析及中西医护理干预措施[J].医学信息,2015,28(38):225.
[15]姜甜,贾友宏,李一石.高血压伴发焦虑和抑郁的研究进展[J].中国分子心脏病学杂志,2015,15(4):1424-1427.
[16]张丽,张振贤,黄瑶.不同慢性应激综合模式下慢性疲劳动物实验研究概况[J].中国中西医结合杂志,2016,36(8):1015-1020.
[17]Dong P,Zhao J,Li N,et al.Sevoflurane exaggerates cognitive decline in a rat model of chronic intermittent hypoxia by aggravating microglia-mediated neuroinflammation via downregulation of PPAR-γin the hippocampus[J].Behav Brain Res,2018,347:325-331.
[18]Cao Y,Li Q.The variation of the 5-hydroxytryptamine system between chronic unpredictable mild stress rats and chronic fatigue syndrome rats induced by forced treadmill running[J].Neuroreport,2017,28(11):630-637.
[19]Liu Z,Wu Y,Liu T,et al.Serotonin regulation in a rat model of exercise-induced chronic fatigue[J].Neuroscience,2017,349:27-34.
[20]Ishida J,Saitoh M,Doehner W,et al.Animal models of cachexia and sarcopenia in chronic illness:cardiac function,body composition changes and therapeutic results[J].Int J Cardiol,2017,238:12-18.
[21]古春青,王著敏,赵铎.红景天对复合应激因素致慢性疲劳大鼠体重及一般行为学的影响[J].中国处方药,2016,14(4):29-30.
[22]Farhan M,Ikram H,Kanwal S.Unpredictable chronic mild stress induced behavioral deficits:A comparative study in male and female rats[J].Pharm Sci,2014,27(4):879-884.
[23]余曦明,陈思源,余望贻.中医慢性疲劳综合症动物模型的建立与评价[J].基础医学,2014,4(2):22-24.