~(60)Co-γ射线辐射对NaCl胁迫下胡杨种子萌发的影响
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摘要
为探讨不同辐射剂量对胡杨种子萌发的影响,筛选有助于提高胡杨种子耐盐性的辐射剂量,以新疆甘家湖采集的胡杨种子为材料,进行~(60)Co-γ射线辐射处理,辐射剂量分别为0(CK)、50、100、150、200Gy,研究辐射处理对不同NaCl浓度胁迫(0、25、50、150、250、300 mmol·L~(-1))下胡杨种子各发芽指标值和累计发芽率的影响。结果表明,在150 Gy辐射处理下,不同NaCl浓度胁迫下的平均发芽率、平均发芽势及平均相对发芽率高于其他处理,且相对盐害率降低;不同~(60)Co-γ辐射处理下的胡杨种子相对发芽率与NaCl浓度间存在极显著负相关关系;~(60)Co-γ射线辐射对NaCl胁迫下种子萌发的耐盐临界浓度为119～181 mmol·L~(-1);150 Gy为有助于提高胡杨种子耐盐性能的最适辐射剂量。本研究结果为胡杨种子的辐射育种技术的应用提供了一定的理论依据和技术支撑。
In order to investigate the influences of different irradiation dosages on the germination of Populus euphratica seeds,and select the optimal radiation dosage for improving the salt resistance of Populus euphratica.P.euphratica seeds collected from Ganjia Lake in Xinjiang were used as material to study the germination indexes and rates at different doses with 0,50,100,150 and 200 Gy under different concentration of NaCl stress.The results indicated that the germination rate,germination potentiality and relative germination rate at 150 Gy radiation treatment were significantly higher than those with other treatments,and the relative salt damage rate was decreased.The germination rate at different doses showed significantly negative correlation with the salt concentration,the critical concentration of NaCl stress was 119-181 mmol·L~(-1)for seeds germination with~(60)Co-γray irradiation,150 Gy was the optimal dose for improving the tolerance to the NaCl stress.The result provides a reference for the application of irradiation breeding in P.euphratica.
In order to investigate the influences of different irradiation dosages on the germination of Populus euphratica seeds,and select the optimal radiation dosage for improving the salt resistance of Populus euphratica.P.euphratica seeds collected from Ganjia Lake in Xinjiang were used as material to study the germination indexes and rates at different doses with 0,50,100,150 and 200 Gy under different concentration of NaCl stress.The results indicated that the germination rate,germination potentiality and relative germination rate at 150 Gy radiation treatment were significantly higher than those with other treatments,and the relative salt damage rate was decreased.The germination rate at different doses showed significantly negative correlation with the salt concentration,the critical concentration of NaCl stress was 119-181 mmol·L~(-1)for seeds germination with~(60)Co-γray irradiation,150 Gy was the optimal dose for improving the tolerance to the NaCl stress.The result provides a reference for the application of irradiation breeding in P.euphratica.
引文
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[19]Rosmala A,Khumaida N,Sukma D.Alteration of leaf anatomy of handeuleum(Graptophyllum pictum,L.Griff)due to gamma irradiation[J].Hayati Journal of Biosciences,2016,23(3):138-142
[20]耿兴敏,王良桂,李娜,杨秀莲.60Co-γ辐射对桂花种子萌发及幼苗生长的影响[J].核农学报,2016,30(2):216-223
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[23]高睿,马淼,赵红艳.60Co-γ辐射对光果甘草种子耐盐性影响的研究[J].农学学报,2016,6(7):77-82
[24]邵金彩,刘玉霞,杨佳鑫,徐兴龙,张启翔,李庆卫.盐胁迫对蜡梅种子萌发及幼苗生长的影响[J].浙江农业学报,2017,29(7):1139-1143
[25]Song J,Feng G,Tian C,Zhang F.Strategies for adaptation of Suaeda physophora,Haloxylon ammodend ron and Haloxylon persicum to a saline enviroment during seed gernination stage[J].Annals of Botany,2005,96(3):399-405
[26]赵东晓,董亚茹,杜建勋,杜建勋,顾寅钰,陈传杰,孙景诗.60Co-γ射线辐射对Na Cl胁迫下海滨锦葵种子萌发的影响[J].山西农业科学,2017,45(10):1602-1605
[27]韩亚楠,吴琼,高睿,马淼,赵红艳.60Co-γ辐照对Na2SO4胁迫下乌拉尔甘草种子发芽特性的影响[J].草业科学,2015,32(3):421-426
[28]刘秀清,章铁.60Co-γ射线低剂量辐照对生菜种子萌发、幼苗生长及酶活性的影响[J].核农学报,2012,26(6):868-872
[29]Beyaz R,Kahramanogullari C T,Yildiz C,Darcin E S,Yildiz M.The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss.under in vitro conditions[J].Journal of Environmental Radioactivity,2016,162-163:129-133
[2]Hao J Q,Yue N,Zheng C X.Selecting the optimum conditions for two-dimensional difference gel electrophoresis of proteins expressed in Populus euphratica[J].Genetics&Molecular Research,2016,15(2):22
[3]刘倩雯,李景文.额济纳绿洲胡杨种子萌发及幼苗生长影响因子[D].北京:北京林业大学,2011
[4]Zhang J,Feng J,Lu J,Yang Y,Zhang X,Wan D,Liu J.Transcriptome differences between two sister desert poplar species under salt stress[J].BMC Genomics,2014,15(1):337
[5]王世绩.全球胡杨林的现状及保护和恢复对策[J].世界林业研究,1996(6):37-44
[6]Ling H,Zhang P,Xu H,Zhao X.How to Regenerate and protect desert riparian Populus euphratica forest in arid areas[J].Scientific Reports,2015,5(154):81-89
[7]Chen J,Zhang J,Hu J,Xiong W,Du C,Lu M.Integrated regulatory network reveals the early salt tolerance mechanism of Populus euphratica[J].Scientific Reports,2017,7(1):6769
[8]韩路,王家强,王海珍,于振荣.塔里木河上游胡杨种群结构与动态[J].生态学报,2014,34(16):21
[9]伍维模,李志军,罗青红,韩路.土壤水分胁迫对胡杨、灰叶胡杨光合作用-光响应特性的影响[J].林业科学,2007,43(5):30-35
[10]买尔燕古丽·阿不都热合曼,艾里西尔·库尔班,玉米提·哈力克,段含明.胡杨雌、雄树物候特征差异比较研究[J].干旱区资源与环境,2012,26(10):97-102
[11]伊丽米努尔,木巴热克·阿尤普,雪合拉提·伊明诺夫,卡依拉·阿布都艾尼.不同种源胡杨(Populus euphratica)种子萌发对盐胁迫的响应以及与生态因子的关系[J].西北林学院学报,2017,32(4):13-21
[12]刘艳萍,李利民,周小明,周登攀,张丽.超干处理对胡杨种子抗逆生理生化指标的影响[J].西北植物学报,2017,37(7):1357-1363
[13]陈子元.从辐射育种的发展来展望航天中的前景[J].核农学报,2002,16(5):261-263
[14]孙丽娜.60Co-γ射线辐照百合诱变育种的研究[D].南京:南京林业大学,2009
[15]阎君,于力,褚云霞,许爽,朱为民.60Co-γ射线对芹菜种子的辐射效应[J].中国农学通报,2011,27(28):205-210
[16]贾彩凤,李艾莲.我国药用植物辐射诱变育种的研究进展[J].中草药,2007,38(4):633-636
[17]李波,李祥莉,赵宇佳,马赫.射线对无芒雀麦种子的辐照效应[J].云南农业大学学报(自然科学版),2017,32(5):756-761
[18]王旭军,吴际友,程勇,廖德志.辐射育种及其在林木育种中的应用前景[J].湖南林业科技,2007,34(2):13-15
[19]Rosmala A,Khumaida N,Sukma D.Alteration of leaf anatomy of handeuleum(Graptophyllum pictum,L.Griff)due to gamma irradiation[J].Hayati Journal of Biosciences,2016,23(3):138-142
[20]耿兴敏,王良桂,李娜,杨秀莲.60Co-γ辐射对桂花种子萌发及幼苗生长的影响[J].核农学报,2016,30(2):216-223
[21]张肖,王旭,焦培培,李志军.胡杨(Populus euphratica)种子萌发及胚生长对盐旱胁迫的响应[J].中国沙漠,2016,36(6):1597-1605
[22]伊丽米努尔,艾力江·麦麦提,卓热木·塔西,Bernd C.Na Cl胁迫下不同种源胡杨种子萌发特性[J].西北林学院学报,2015,30(6):88-94
[23]高睿,马淼,赵红艳.60Co-γ辐射对光果甘草种子耐盐性影响的研究[J].农学学报,2016,6(7):77-82
[24]邵金彩,刘玉霞,杨佳鑫,徐兴龙,张启翔,李庆卫.盐胁迫对蜡梅种子萌发及幼苗生长的影响[J].浙江农业学报,2017,29(7):1139-1143
[25]Song J,Feng G,Tian C,Zhang F.Strategies for adaptation of Suaeda physophora,Haloxylon ammodend ron and Haloxylon persicum to a saline enviroment during seed gernination stage[J].Annals of Botany,2005,96(3):399-405
[26]赵东晓,董亚茹,杜建勋,杜建勋,顾寅钰,陈传杰,孙景诗.60Co-γ射线辐射对Na Cl胁迫下海滨锦葵种子萌发的影响[J].山西农业科学,2017,45(10):1602-1605
[27]韩亚楠,吴琼,高睿,马淼,赵红艳.60Co-γ辐照对Na2SO4胁迫下乌拉尔甘草种子发芽特性的影响[J].草业科学,2015,32(3):421-426
[28]刘秀清,章铁.60Co-γ射线低剂量辐照对生菜种子萌发、幼苗生长及酶活性的影响[J].核农学报,2012,26(6):868-872
[29]Beyaz R,Kahramanogullari C T,Yildiz C,Darcin E S,Yildiz M.The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss.under in vitro conditions[J].Journal of Environmental Radioactivity,2016,162-163:129-133