~(60)Co-γ辐照对不同胡麻品种种子萌发及幼苗生长的影响
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摘要
为探讨辐照对胡麻(Linum usitatissimum)种子的诱变作用,以不同剂量的60Co-γ射线对9个胡麻品种的种子进行辐照处理,观察其对胡麻种子萌发及出苗状况的影响。结果表明,辐照处理对胡麻种子萌发状况的影响存在显著的品种间差异。除了陇亚9号和陇亚14号外,其他7个品种种子发芽率均与辐照剂量呈负相关关系,随着辐照剂量的增加,7个品种胡麻种子的发芽率和相对出苗率明显降低。9个品种胡麻种子的半致死剂量大小顺序为陇亚14号>陇亚13号>定亚17号>天亚10号>陇亚9号>张亚3号>陇亚11号>陇亚10号>陇亚12号,分别为6 668、6 521、6 462、6 417、6 399、5 869、5 786、5 716和5 681 Gy。低剂量(1 000 Gy以下)的辐照能提高陇亚9号和陇亚14号2个品种种子的萌发率及出苗率;高剂量(12 000 Gy以上)辐照后种子虽能萌发,但出苗率受到明显的抑制,出苗率均为0。除张亚3号和陇亚13号外,其他7个品种根长和株高在低剂量辐照时增加,高剂量辐照时减小。本研究结果可为胡麻辐照育种提供依据,促进胡麻育种进程。
To investigate the effects of irradiation on Linum usitatissimum seeds,nine varieties of L.usitatissimum were irradiated with different doses of 60 Co-γ radiation,and the effects of radiation on seed germination and seedling growth were examined.The results indicated that the effects of radiation on seed germination varied among the different varieties.With the exception of Longya 9 and Longya 10,the seed germination rate of the other seven varieties was negatively correlated with radiation dose,and the germination rate and relative emergence rate decreased with increasing dose.Semi-lethal doses for the L.usitatissimum varieties Longya 14,Longya 13,Dingya 17,Tianya 10,Longya 9,Zhangya 3,Longya 11,Longya10,and Longya 12 were 6 668,6 521,6 462,6 417,6 399,5 869,5 786,5 716,and 5 681 Gy,respectively.Lower doses(lower than 1 000 Gy) of radiation increased the germination rate of Longya 9 and Longya 14.At high doses(higher than 12 000 Gy),the rate of germination and seedling growth were markedly inhibited.Although seeds were able to germinate,seedling growth rate was significantly inhibited and the rate was 0.With the exception of Zhangya 3 and Longya 13,the plant height and root length of the other seven varieties increased under low-dose irradiation,and decreased at high dose.The results of this study will provide a theoretical basis for mutation breeding and acceleration of the breeding process of L.usitatissimum.
To investigate the effects of irradiation on Linum usitatissimum seeds,nine varieties of L.usitatissimum were irradiated with different doses of 60 Co-γ radiation,and the effects of radiation on seed germination and seedling growth were examined.The results indicated that the effects of radiation on seed germination varied among the different varieties.With the exception of Longya 9 and Longya 10,the seed germination rate of the other seven varieties was negatively correlated with radiation dose,and the germination rate and relative emergence rate decreased with increasing dose.Semi-lethal doses for the L.usitatissimum varieties Longya 14,Longya 13,Dingya 17,Tianya 10,Longya 9,Zhangya 3,Longya 11,Longya10,and Longya 12 were 6 668,6 521,6 462,6 417,6 399,5 869,5 786,5 716,and 5 681 Gy,respectively.Lower doses(lower than 1 000 Gy) of radiation increased the germination rate of Longya 9 and Longya 14.At high doses(higher than 12 000 Gy),the rate of germination and seedling growth were markedly inhibited.Although seeds were able to germinate,seedling growth rate was significantly inhibited and the rate was 0.With the exception of Zhangya 3 and Longya 13,the plant height and root length of the other seven varieties increased under low-dose irradiation,and decreased at high dose.The results of this study will provide a theoretical basis for mutation breeding and acceleration of the breeding process of L.usitatissimum.
引文
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