毛竹辐射诱变实生优株生长性状的综合评价
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摘要
由于传统的毛竹育种难以获得创新种质,本课题运用60Coγ射线处理毛竹实生种子。2007—2010年在肥西县苗圃开展了苗期试验,并筛选出16个苗期优株;2011—2017年在广德县开展造林试验,以不辐射的竹苗为对照,并对16个优株的6个生长性状指标连续6年进行差异分析、相关性分析;结合主成分分析和隶属函数法对不同优株的优劣性、稳定性进行综合评价。结果表明:6个生长性状指标在不同优株、不同时期下进行双因素方差分析均达到极显著水平;主成分分析将6个单项指标集约于3个主成分,累积贡献率达94.9%;通过隶属函数值D评价优株的生长质量,优CK-1、优10gy-17生长性状最优,结果与造林试验中生长表型相近。本研究为毛竹辐射优株的选择提供1种可行的方法,同时为进一步筛选毛竹辐射诱变出优异基因类型奠定基础。
Because it is difficult to obtain innovative germplasm in traditional bamboo breeding, this topic innovatively uses 60 Co gamma ray to treat seedling seeds of bamboo. From 2007 to 2010 years, the seedling stage test was carried out in Feixi county, and 16 superior seedlings were screened out. Then the afforestation experiment was carried out from 2011 to 2017. The 6 growth traits of 16 superior seedlings were analyzed for 6 consecutive years by using unirradiated bamboo seedlings as the control. Difference analysis, correlation analysis, principal component analysis and subordinate function values analysis were used to evaluate the quality and stability of different superior plants. The results show that The differences of 6 growth indexes were significantly different each other in different periods, and different superior plants with two-factor ANOVA, eight single indexes were grouped into two principal components by principal component analysis, and the cumulative contribution rate was 94.9%; The growth quality of superior plants was evaluated by subordinate function value D; The growth characteristics of CK-1 and superior 10 gy-17 were the best, and these were similar to the growth phenotypes in afforestation experiments. This study provides a feasible method for the selection of excellent radiative plants of P.pubescens, and lays a foundation for further screening of excellent gene types induced by radiation from P. pubescens.
Because it is difficult to obtain innovative germplasm in traditional bamboo breeding, this topic innovatively uses 60 Co gamma ray to treat seedling seeds of bamboo. From 2007 to 2010 years, the seedling stage test was carried out in Feixi county, and 16 superior seedlings were screened out. Then the afforestation experiment was carried out from 2011 to 2017. The 6 growth traits of 16 superior seedlings were analyzed for 6 consecutive years by using unirradiated bamboo seedlings as the control. Difference analysis, correlation analysis, principal component analysis and subordinate function values analysis were used to evaluate the quality and stability of different superior plants. The results show that The differences of 6 growth indexes were significantly different each other in different periods, and different superior plants with two-factor ANOVA, eight single indexes were grouped into two principal components by principal component analysis, and the cumulative contribution rate was 94.9%; The growth quality of superior plants was evaluated by subordinate function value D; The growth characteristics of CK-1 and superior 10 gy-17 were the best, and these were similar to the growth phenotypes in afforestation experiments. This study provides a feasible method for the selection of excellent radiative plants of P.pubescens, and lays a foundation for further screening of excellent gene types induced by radiation from P. pubescens.
引文
[1]陈光才,马乃训.竹子遗传育种研究进展[J].林业科学研究,2005, 18(6):749-754.
[2]刘凤,曹帮华,蔡春菊,等.毛竹种子萌发过程中生理生化特性变化的研究[J].种子, 2009, 28(2):12-14.
[3]谢庆华,邢溪燕,谭汝学.毛竹种子组培技术初步研究[J].林业科技通讯, 2000(12):18-20.
[4]秦忠林.毛竹种子育苗造林技术[J].农技服务, 2009, 26(9):98-99, 125.
[5]徐冠仁.植物诱变育种学[M].北京:中国农业出版社,1996:1-3.
[6]刘璐璐,柴明良,徐晓薇,等.γ射线辐照对春兰根状茎生长及抗氧化酶活性的影响[J].核农学报, 2008, 22(1):23-27.
[7]孙丽娜.60Coγ射线辐照百合诱变育种研究[D].南京:南京林业大学, 2009.
[8]刘丽强,刘军丽,张杰,等. 60Coγ辐射对观赏海棠组培苗的诱变效应[J].中国农业科学, 2010, 43(20):4255-4264.
[9]高健.60Coγ射线辐照中国水仙的诱变效应和机理研究[D].北京:北京林业科学研究院, 2000.
[10]潘宏.兰花辐射诱变与组织培养技术初步研究[D].福州:福建农林大学, 2008.
[11]彭绿春,黄丽萍,余朝秀,等.四种兰花辐射诱变育种研究初报[J].云南农业大学学报, 2007, 22(3):333-336.
[12]王旭东,吴际友,程勇,等.辐射育种及其在林木育种中的应用前景[J].湖南林业科技, 2007, 34(2):13-15.
[13]蔡新玲,吴中能,于一苏,等.毛竹辐射诱变育苗的研究初报[J].安徽农业科学, 2008, 36(17):7244-7246.
[14]徐永杰,邓先珍,代新平,等.基于主成分分析和聚类分析的油桐优良家系抗性综合评价[J].中南林业科技大学学报,2012, 32(7):24-27.
[15]许桂芳,张朝阳,向佐湘.利用隶属函数法对4种珍珠菜属植物的抗寒性综合评价[J].西北林学院学报,2009,24(3):24-26.
[16]王军,周美学,许如根,等.大麦耐湿性鉴定指标和评价方法研究[J].中国农业科学, 2007, 40(10):2145-2152.
[17]周根土.安徽主要用材树种造林与经营[M].北京:中国林业出版社, 2011.
[18]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
[19]李长江,温晓霞,孙渭,等.陕南主栽烟草品种化学成分综合评价与分析[J].西北农林科技大学学报(自然科学版),2013,41(7):67-74.
[20]曾小玲,方淑桂,陈文辉,等.不同基因型菜心耐湿性综合评价[J].热带作物学报,2010,31(4):572-576.
[21]孟凡珍,张振贤,于贤昌.不同生态型结球大白菜抗寒性的评价[J].中国农业大学学报,2004,9(4):35-39.
[22]曹志华,吴中能,王云,等.不同竹种耐湿性生理指标综合评价及其预测[J].江苏农业科学, 2015, 43(4):203-205.
[23]黄滔,唐红,刘玮,等.长沙地区3种优良观赏竹发笋及幼竹高生长规律[J].经济林研究, 2016, 34(2):114-119.
[24]孟勇,艾文胜,杨明,等.上阔下竹复合经营模式对毛竹生长的影响[J].经济林研究, 2016, 34(3):135-141.
[25]蔡新玲,刘俊龙,苗婷婷,等.毛竹辐射诱变实生苗期选择研究[J].江西农业学报2015, 27(6):12-16.
[2]刘凤,曹帮华,蔡春菊,等.毛竹种子萌发过程中生理生化特性变化的研究[J].种子, 2009, 28(2):12-14.
[3]谢庆华,邢溪燕,谭汝学.毛竹种子组培技术初步研究[J].林业科技通讯, 2000(12):18-20.
[4]秦忠林.毛竹种子育苗造林技术[J].农技服务, 2009, 26(9):98-99, 125.
[5]徐冠仁.植物诱变育种学[M].北京:中国农业出版社,1996:1-3.
[6]刘璐璐,柴明良,徐晓薇,等.γ射线辐照对春兰根状茎生长及抗氧化酶活性的影响[J].核农学报, 2008, 22(1):23-27.
[7]孙丽娜.60Coγ射线辐照百合诱变育种研究[D].南京:南京林业大学, 2009.
[8]刘丽强,刘军丽,张杰,等. 60Coγ辐射对观赏海棠组培苗的诱变效应[J].中国农业科学, 2010, 43(20):4255-4264.
[9]高健.60Coγ射线辐照中国水仙的诱变效应和机理研究[D].北京:北京林业科学研究院, 2000.
[10]潘宏.兰花辐射诱变与组织培养技术初步研究[D].福州:福建农林大学, 2008.
[11]彭绿春,黄丽萍,余朝秀,等.四种兰花辐射诱变育种研究初报[J].云南农业大学学报, 2007, 22(3):333-336.
[12]王旭东,吴际友,程勇,等.辐射育种及其在林木育种中的应用前景[J].湖南林业科技, 2007, 34(2):13-15.
[13]蔡新玲,吴中能,于一苏,等.毛竹辐射诱变育苗的研究初报[J].安徽农业科学, 2008, 36(17):7244-7246.
[14]徐永杰,邓先珍,代新平,等.基于主成分分析和聚类分析的油桐优良家系抗性综合评价[J].中南林业科技大学学报,2012, 32(7):24-27.
[15]许桂芳,张朝阳,向佐湘.利用隶属函数法对4种珍珠菜属植物的抗寒性综合评价[J].西北林学院学报,2009,24(3):24-26.
[16]王军,周美学,许如根,等.大麦耐湿性鉴定指标和评价方法研究[J].中国农业科学, 2007, 40(10):2145-2152.
[17]周根土.安徽主要用材树种造林与经营[M].北京:中国林业出版社, 2011.
[18]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
[19]李长江,温晓霞,孙渭,等.陕南主栽烟草品种化学成分综合评价与分析[J].西北农林科技大学学报(自然科学版),2013,41(7):67-74.
[20]曾小玲,方淑桂,陈文辉,等.不同基因型菜心耐湿性综合评价[J].热带作物学报,2010,31(4):572-576.
[21]孟凡珍,张振贤,于贤昌.不同生态型结球大白菜抗寒性的评价[J].中国农业大学学报,2004,9(4):35-39.
[22]曹志华,吴中能,王云,等.不同竹种耐湿性生理指标综合评价及其预测[J].江苏农业科学, 2015, 43(4):203-205.
[23]黄滔,唐红,刘玮,等.长沙地区3种优良观赏竹发笋及幼竹高生长规律[J].经济林研究, 2016, 34(2):114-119.
[24]孟勇,艾文胜,杨明,等.上阔下竹复合经营模式对毛竹生长的影响[J].经济林研究, 2016, 34(3):135-141.
[25]蔡新玲,刘俊龙,苗婷婷,等.毛竹辐射诱变实生苗期选择研究[J].江西农业学报2015, 27(6):12-16.