罕见B(A)02亚型的血清学特性及基因表达分析
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摘要
目的留取临床血清学表现为Aw B的血标本进行血型血清学、基因序列检测,探讨其基因表达机制,为临床安全输血提供理论基础。方法采用常规试管凝集法检测ABO正反定型,采用PCR-SSP法进行其基因分型并测序。结果 3例样本常规血清学表现红细胞与抗-A w～2+凝集,与抗-B 4+凝集,与抗-A1不凝集,反定型含有抗-A抗体; PCR-SSP法检测标本基因型均为B(A)型,测序发现均在700位核苷酸发生C> G的单碱基突变。结论经血清学和基因测序证实,3例为B(A)表现型,并携带有B(A) 02等位基因。
Objective Aw B blood sample was collected for serological detection and gene sequencing,and its gene expression mechanism was explored,so as to provide theoretical basis for safe blood transfusion. Methods ABO phenotypes were detected by conventional tube agglutination test with positive and negative stereotypes. ABO genotypes were detected by PCR-SSP and sequencing. Results The routine serological results of 3 samples showed agglutination of erythrocytes with anti-A w-2 +,agglutination with anti-B 4 +,non-agglutination with anti-A1 and anti-A antibody in reverse. PCR-SSP was used to detect the genotype. All of them were B( A) type. The single nucleotide mutation occurred at 700 C > G by sequencing.Conclusion Serological and genetic sequencing confirmed that all three cases were B( A) phenotype carrying B( A) 02 allele.
Objective Aw B blood sample was collected for serological detection and gene sequencing,and its gene expression mechanism was explored,so as to provide theoretical basis for safe blood transfusion. Methods ABO phenotypes were detected by conventional tube agglutination test with positive and negative stereotypes. ABO genotypes were detected by PCR-SSP and sequencing. Results The routine serological results of 3 samples showed agglutination of erythrocytes with anti-A w-2 +,agglutination with anti-B 4 +,non-agglutination with anti-A1 and anti-A antibody in reverse. PCR-SSP was used to detect the genotype. All of them were B( A) type. The single nucleotide mutation occurred at 700 C > G by sequencing.Conclusion Serological and genetic sequencing confirmed that all three cases were B( A) phenotype carrying B( A) 02 allele.
引文
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[12]陶翠华,涂茹.罕见B(A)亚型的血清学特点及分子机制的探究[J].临床血液学杂志,2015,28(4):286-288.
[13]李丽春,章旭,李剑平.罕见B(A)血型的鉴定及临床安全输血的研究[J].中国输血杂志,2016,29(7):689-690.
[14]方乐,肖建宇,吴敏慧,等. ABO亚型B(A)04的鉴定及分子机制研究[J].临床输血与检验杂志,2016,18(5):424-427.
[15] Chen Q,Li JH,Xiao JY,et al. Molecular geneticanalysis and structure model of a rare B(A)02 subgroup of the ABO blood group system[J]. Transfus Apher Sci,2014,51(2):203-208.
[16]林甲进,施顺秋,朱碎永,等.血型系统中罕见B(A)亚型的鉴定及血清学特性分析[J].中国卫生检验杂志,2016,26(8):1149-1150.
[17] Guo ZH,Xiang D,Zhu ZY,et al. Serologic and molecular characterizationof the B(A)blood group in the Chinese population[J].Immunohematology,2007,23(2):69-74.
[2]王晓华,刘衍春,李兴华,等.《中国输血杂志》2006-2015年红细胞血型参比文献回顾性分析[J].中国输血杂志,2017,30(4):408-410.
[3]韩蕊,刘裔军,张明娟,等. A亚型的血型鉴定与安全输血[J].中国卫生检验杂志[J],2015,25(14):2446-2448.
[4]黄颖,林甲进,朱碎永,等. A2亚型的血清学和分子生物学的对比分析[J].中国卫生检验杂志,2017,27(4):518-520.
[5]朱于莉,金肖君,胡彬,等.青岛及周边地区A3、B3亚型的分子机制研究[J].医学分子生物学杂志,2017,14(2):73-74.
[6]林甲进,朱碎永,施顺秋,等. A2B亚型血型血清学特征及采血前后主要血液指标的变化[J].中国卫生检验杂志,2013,23(12):2623-2624.
[7]邹洁,陈泽惠,沈钢.一例ABO亚型B(A)04个体及其家系成员的分子遗传学分析[J].中华医学遗传学杂志,2017,34(2):251-254.
[8]蔡雪娇,洪俊英,谢作听. ABO血型正反定型不一致的血清学和分子生物学研究[J].中国卫生检验杂志,2017,27(12):1717-1718
[9]章尤权,蔡晓红,王清泰,等. cis AB01等位基因的鉴定及分子机制研究[J].中华检验医学杂志,2016,39(10):786-787.
[10] Yamamoto F,Mc Neill PD,Yamamoto M,et al. Molecular genetic analysis of the ABO blood group system3. A(X)and B(A)alleles[J]. Vox Sang,1993,64(3):171-174.
[11] Seltsam A,Hallensleben M,Kollman A,et al. Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles[J]. Trans Fusion,2003,43(4):428-439.
[12]陶翠华,涂茹.罕见B(A)亚型的血清学特点及分子机制的探究[J].临床血液学杂志,2015,28(4):286-288.
[13]李丽春,章旭,李剑平.罕见B(A)血型的鉴定及临床安全输血的研究[J].中国输血杂志,2016,29(7):689-690.
[14]方乐,肖建宇,吴敏慧,等. ABO亚型B(A)04的鉴定及分子机制研究[J].临床输血与检验杂志,2016,18(5):424-427.
[15] Chen Q,Li JH,Xiao JY,et al. Molecular geneticanalysis and structure model of a rare B(A)02 subgroup of the ABO blood group system[J]. Transfus Apher Sci,2014,51(2):203-208.
[16]林甲进,施顺秋,朱碎永,等.血型系统中罕见B(A)亚型的鉴定及血清学特性分析[J].中国卫生检验杂志,2016,26(8):1149-1150.
[17] Guo ZH,Xiang D,Zhu ZY,et al. Serologic and molecular characterizationof the B(A)blood group in the Chinese population[J].Immunohematology,2007,23(2):69-74.