两种极端嗜热古菌对黄铜矿的吸附和浸出行为（英文）
|
摘要
研究两株具有代表性的极端嗜热古菌A. brierleyi和S. metallicus及其混合物对黄铜矿的吸附和浸出行为。结果表明,S.metallicus的铜浸出率略高于A.brierleyi的,其中混合菌体系的铜浸出率最高。浸出过程中的群落结构分析表明,S. metallicus在菌群中属于优势菌群;A. brierleyi所占比例呈现上升趋势,而这一上升趋势与黄铜矿在混合体系浸出后期的铜浓度比在单菌体系增长更快有关。Langmuir参数分析得出两种古菌之间不存在竞争性吸附关系,而qPCR结果显示,在混合浸出的过程中,S. metallicus和A. brierleyi之间会产生促进性吸附。本研究进一步阐明混合极端嗜热菌在矿物表面上所发生的吸附行为。
The adsorption and leaching of chalcopyrite by two extreme thermophilic archaea (A. brierleyi and S. metallicus) and their mixture were studied. The results revealed that the chalcopyrite leaching rate of S. metallicus was slightly higher than that of A. brierleyi; the mixed system showed the highest rate. Community structure analysis during the leaching process showed that S. metallicus was maintained in a predominant state. However, the proportion of A. brierleyi in the community increased during leaching. Copper concentrations, which increased faster in the mixed system than in the single-organism systems during later stages, was related to the change of A. brierleyi in the community. Langmuir parameter analysis revealed no competitive adsorption between these two thermophilic archaea. Furthermore, qPCR (quantitative polymerase chain reaction) confirmed that adsorption was promoted between A. brierleyi and S. metallicus during mixed leaching. These findings can improve our understanding of the adsorption behaviors of mixed extreme microbial populations on mineral surfaces.
The adsorption and leaching of chalcopyrite by two extreme thermophilic archaea (A. brierleyi and S. metallicus) and their mixture were studied. The results revealed that the chalcopyrite leaching rate of S. metallicus was slightly higher than that of A. brierleyi; the mixed system showed the highest rate. Community structure analysis during the leaching process showed that S. metallicus was maintained in a predominant state. However, the proportion of A. brierleyi in the community increased during leaching. Copper concentrations, which increased faster in the mixed system than in the single-organism systems during later stages, was related to the change of A. brierleyi in the community. Langmuir parameter analysis revealed no competitive adsorption between these two thermophilic archaea. Furthermore, qPCR (quantitative polymerase chain reaction) confirmed that adsorption was promoted between A. brierleyi and S. metallicus during mixed leaching. These findings can improve our understanding of the adsorption behaviors of mixed extreme microbial populations on mineral surfaces.
引文
[1]LI Y,KAWASHIMA N,LI J,CHANDRA A,GERSON A.A review of the structure,and fundamental mechanisms and kinetics of the leaching of chalcopyrite[J].Advances in Colloid and Interface Science,2013,197:1-32.
[2]PATHAK A,MORRISON L,HEALY M G.Catalytic potential of selected metal ions for bioleaching,and potential techno-economic and environmental issues:A critical review[J].Bioresource Technology,2017,229:211-221.
[3]DONATI E R,CASTRO C,URBIETA M S.Thermophilic microorganisms in biomining[J].World Journal of Microbiology and Biotechnology,2016,32(11):1-8.
[4]CLARK M E,BATTY J D,VAN BUUREN C B,DEW D W,EAMON M A.Biotechnology in minerals processing:Technological breakthroughs creating value[J].Hydrometallurgy,2006,83(1-4):3-9.
[5]WATLING H R.The bioleaching of sulphide minerals with emphasis on copper sulphides-A review[J].Hydrometallurgy,2006,84(1-2):81-108.
[6]COUNTS J A,ZELDES B M,LEE L L,STRAUB C T,ADAMS MW W,KELLY R M.Physiological,metabolic and biotechnological features of extremely thermophilic microorganisms[J].Wiley Interdisciplinary Reviews:Systems Biology and Medicine,2017,9(3):1-23.
[7]RUIZ L M,VALENZUELA S,CASTRO M,GONZALEZ A,FREZZA M,SOULèRE L,ROHWERDER T,QUENEAU Y,DOUTHEAU A,SAND W,JEREZ C A,GUILIANI N.AHLcommunication is a widespread phenomenon in biomining bacteria and seems to be involved in mineral-adhesion efficiency[J].Hydrometallurgy,2008,94(1-4):133-137.
[8]BERG I A,KOCKELKORN D,RAMOS-VERA W H,SAY R F,ZARZYCKI J,HüGLER M,ALBER B,FUCHS G.Autotrophic carbon fixation in archaea[J].Nature Reviews:Microbiology,2010,8(6):447-460.
[9]LING Wei-bo,WANG Lei,LIU Hong-chang,NIE Zhen-yuan,YANG Yun,YANG Yi,MA Chen-yan,ZHENG Lei,ZHAO Yi-dong,XIA Jin-lan.The evidence of decisive effect of both surface microstructure and speciation of chalcopyrite on attachment behaviors of extreme thermoacidophile Sulfolobus metallicus[J].Minerals,2018,8(4):159-173.
[10]CáRDENAS J P,QUATRINI R,HOLMES D S.Genomic and metagenomic challenges and opportunities for bioleaching:Amini-review[J].Research in Microbiology,2016,167(7):529-538.
[11]KADNIKOV V V,IVASENKO D A,BELETSKY A V,MARDANOV A V,DANILOVA E V,PIMENOV N V,KARNACHUK O V,RAVIN N V.Effect of metal concentration on the microbial community in acid mine drainage of a polysulfide ore deposit[J].Microbiology,2016,85(6):745-751.
[12]ZHAO Hong-bo,WANG Jun,GAN Xiao-wen,ZHENG Xi-hua,TAO Lang,HU Ming-hao,LI Yi-ni,QIN Wen-qing,QIU Guan-zhou.Effects of pyrite and bornite on bioleaching of two different types of chalcopyrite in the presence of Leptospirillum ferriphilum[J].Bioresource Technology,2015,194:28-35.
[13]NI G,CHRISTEL S,ROMAN P,WONG Z L,BIJMANS M F M,DOPSON M.Electricity generation from an inorganic sulfur compound containing mining wastewater by acidophilic microorganisms[J].Research in Microbiology,2016,167(7):568-575.
[14]WHEATON G,COUNTS J,MUKHERJEE A,KRUH J,KELLY R.The confluence of heavy metal biooxidation and heavy metal resistance:Implications for bioleaching by extreme thermoacidophiles[J].Minerals,2015,5(3):397-451.
[15]GUMULYA Y,BOXALL N,KHALEQUE H,SANTALA V,CARLSON R P,KAKSONEN A H.In a quest for engineering acidophiles for biomining applications:Challenges and opportunities[J].Genes,2018,9(2):116-144.
[16]XIAO Yun-hua,XU Yong-Dong,DONG Wei-ling,LIANG Yi-li,FAN Fen-liang,ZHANG Xiao-xia,ZHANG Xian,NIU Jiao-jiao,MA Li-yuan,SHE Si-yuan.The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system[J].Applied Microbiology and Biotechnology,2015,99(23):10311-10322.
[17]JOHNSON D B.Biodiversity and ecology of acidophilic microorganisms[J].FEMS Microbiology Ecology,1998,27(4):307-317.
[18]JOHNSON D B.Biodiversity and interactions of acidophiles:Key to understanding and optimizing microbial processing of ores and concentrates[J].Transactions of Nonferrous Metals Society of China,2008,18(6):1367-1373.
[19]QIN Wen-qing,YANG Cong-ren,LAI Shao-shi,WANG Jun,LIUKai,ZHANG Bo.Bioleaching of chalcopyrite by moderately thermophilic microorganisms[J].Bioresource Technology,2013,129:200-208.
[20]FENG Shou-shuai,YANG Hai-lin,WANG Wu.Microbial community succession mechanism coupling with adaptive evolution of adsorption performance in chalcopyrite bioleaching[J].Bioresource Technology,2015,191:37-44.
[21]LIANG Yu-ting,ZHU Shan,WANG Jun,AI Chen-bing,QINWen-qing.Adsorption and leaching of chalcopyrite by Sulfolobus metallicus YN24 cultured in the distinct energy sources[J].International Journal of Minerals,Metallurgy,and Materials,2015,22(6):549-552.
[22]VILCáEZ J,YAMADA R,INOUE C.Effect of pH reduction and ferric ion addition on the leaching of chalcopyrite at thermophilic temperatures[J].Hydrometallurgy,2009,96(1-2):62-71.
[23]FRANZMANN P D,HADDAD C M,HAWKES R B,ROBERTSONW J,PLUMB J J.Effects of temperature on the rates of iron and sulfur oxidation by selected bioleaching Bacteria and archaea:Application of the Ratkowsky equation[J].Minerals Engineering,2005,18(13-14):1304-1314.
[24]VILCáEZ J,SUTO K,INOUE C.Bioleaching of chalcopyrite with thermophiles:Temperature-pH-ORP dependence[J].International Journal of Mineral Processing,2008,88(1-2):37-44.
[25]ZHU Wei,XIA Jin-lan,PENG An-an,NIE Zhen-yuan,QIUGuan-zhou.Characterization of apparent sulfur oxidation activity of thermophilic archaea in bioleaching of chalcopyrite[J].Transactions of Nonferrous Metals Society of China,2013,23(8):2383-2388.
[26]ZENG Wei-min,QIU Guan-zhou,ZHOU Hong-bo,PENG Juan-hua,CHEN Miao,TAN Su Nee,CHAO Wei-liang,LIU Xue-duan,ZHANG Yan-sheng.Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate[J].Bioresource Technology,2010,101(18):7068-7075.
[27]ZHU Wei,XIA Jin-lan,YANG Yi,NIE Zhen-yuan,PENG An-an,LIU Hong-chang,QIU Guan-zhou.Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite[J].Bioresource Technology,2013,133:405-413.
[28]SONG Jian,LIN Jian-qun,REN Yi-lin,LIN Jian-qiang.Competitive adsorption of binary mixture of Leptospirillum ferriphilum and Acidithiobacillus caldus onto pyrite[J].Biotechnology and Bioprocess Engineering,2010,15(6):923-930.
[2]PATHAK A,MORRISON L,HEALY M G.Catalytic potential of selected metal ions for bioleaching,and potential techno-economic and environmental issues:A critical review[J].Bioresource Technology,2017,229:211-221.
[3]DONATI E R,CASTRO C,URBIETA M S.Thermophilic microorganisms in biomining[J].World Journal of Microbiology and Biotechnology,2016,32(11):1-8.
[4]CLARK M E,BATTY J D,VAN BUUREN C B,DEW D W,EAMON M A.Biotechnology in minerals processing:Technological breakthroughs creating value[J].Hydrometallurgy,2006,83(1-4):3-9.
[5]WATLING H R.The bioleaching of sulphide minerals with emphasis on copper sulphides-A review[J].Hydrometallurgy,2006,84(1-2):81-108.
[6]COUNTS J A,ZELDES B M,LEE L L,STRAUB C T,ADAMS MW W,KELLY R M.Physiological,metabolic and biotechnological features of extremely thermophilic microorganisms[J].Wiley Interdisciplinary Reviews:Systems Biology and Medicine,2017,9(3):1-23.
[7]RUIZ L M,VALENZUELA S,CASTRO M,GONZALEZ A,FREZZA M,SOULèRE L,ROHWERDER T,QUENEAU Y,DOUTHEAU A,SAND W,JEREZ C A,GUILIANI N.AHLcommunication is a widespread phenomenon in biomining bacteria and seems to be involved in mineral-adhesion efficiency[J].Hydrometallurgy,2008,94(1-4):133-137.
[8]BERG I A,KOCKELKORN D,RAMOS-VERA W H,SAY R F,ZARZYCKI J,HüGLER M,ALBER B,FUCHS G.Autotrophic carbon fixation in archaea[J].Nature Reviews:Microbiology,2010,8(6):447-460.
[9]LING Wei-bo,WANG Lei,LIU Hong-chang,NIE Zhen-yuan,YANG Yun,YANG Yi,MA Chen-yan,ZHENG Lei,ZHAO Yi-dong,XIA Jin-lan.The evidence of decisive effect of both surface microstructure and speciation of chalcopyrite on attachment behaviors of extreme thermoacidophile Sulfolobus metallicus[J].Minerals,2018,8(4):159-173.
[10]CáRDENAS J P,QUATRINI R,HOLMES D S.Genomic and metagenomic challenges and opportunities for bioleaching:Amini-review[J].Research in Microbiology,2016,167(7):529-538.
[11]KADNIKOV V V,IVASENKO D A,BELETSKY A V,MARDANOV A V,DANILOVA E V,PIMENOV N V,KARNACHUK O V,RAVIN N V.Effect of metal concentration on the microbial community in acid mine drainage of a polysulfide ore deposit[J].Microbiology,2016,85(6):745-751.
[12]ZHAO Hong-bo,WANG Jun,GAN Xiao-wen,ZHENG Xi-hua,TAO Lang,HU Ming-hao,LI Yi-ni,QIN Wen-qing,QIU Guan-zhou.Effects of pyrite and bornite on bioleaching of two different types of chalcopyrite in the presence of Leptospirillum ferriphilum[J].Bioresource Technology,2015,194:28-35.
[13]NI G,CHRISTEL S,ROMAN P,WONG Z L,BIJMANS M F M,DOPSON M.Electricity generation from an inorganic sulfur compound containing mining wastewater by acidophilic microorganisms[J].Research in Microbiology,2016,167(7):568-575.
[14]WHEATON G,COUNTS J,MUKHERJEE A,KRUH J,KELLY R.The confluence of heavy metal biooxidation and heavy metal resistance:Implications for bioleaching by extreme thermoacidophiles[J].Minerals,2015,5(3):397-451.
[15]GUMULYA Y,BOXALL N,KHALEQUE H,SANTALA V,CARLSON R P,KAKSONEN A H.In a quest for engineering acidophiles for biomining applications:Challenges and opportunities[J].Genes,2018,9(2):116-144.
[16]XIAO Yun-hua,XU Yong-Dong,DONG Wei-ling,LIANG Yi-li,FAN Fen-liang,ZHANG Xiao-xia,ZHANG Xian,NIU Jiao-jiao,MA Li-yuan,SHE Si-yuan.The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system[J].Applied Microbiology and Biotechnology,2015,99(23):10311-10322.
[17]JOHNSON D B.Biodiversity and ecology of acidophilic microorganisms[J].FEMS Microbiology Ecology,1998,27(4):307-317.
[18]JOHNSON D B.Biodiversity and interactions of acidophiles:Key to understanding and optimizing microbial processing of ores and concentrates[J].Transactions of Nonferrous Metals Society of China,2008,18(6):1367-1373.
[19]QIN Wen-qing,YANG Cong-ren,LAI Shao-shi,WANG Jun,LIUKai,ZHANG Bo.Bioleaching of chalcopyrite by moderately thermophilic microorganisms[J].Bioresource Technology,2013,129:200-208.
[20]FENG Shou-shuai,YANG Hai-lin,WANG Wu.Microbial community succession mechanism coupling with adaptive evolution of adsorption performance in chalcopyrite bioleaching[J].Bioresource Technology,2015,191:37-44.
[21]LIANG Yu-ting,ZHU Shan,WANG Jun,AI Chen-bing,QINWen-qing.Adsorption and leaching of chalcopyrite by Sulfolobus metallicus YN24 cultured in the distinct energy sources[J].International Journal of Minerals,Metallurgy,and Materials,2015,22(6):549-552.
[22]VILCáEZ J,YAMADA R,INOUE C.Effect of pH reduction and ferric ion addition on the leaching of chalcopyrite at thermophilic temperatures[J].Hydrometallurgy,2009,96(1-2):62-71.
[23]FRANZMANN P D,HADDAD C M,HAWKES R B,ROBERTSONW J,PLUMB J J.Effects of temperature on the rates of iron and sulfur oxidation by selected bioleaching Bacteria and archaea:Application of the Ratkowsky equation[J].Minerals Engineering,2005,18(13-14):1304-1314.
[24]VILCáEZ J,SUTO K,INOUE C.Bioleaching of chalcopyrite with thermophiles:Temperature-pH-ORP dependence[J].International Journal of Mineral Processing,2008,88(1-2):37-44.
[25]ZHU Wei,XIA Jin-lan,PENG An-an,NIE Zhen-yuan,QIUGuan-zhou.Characterization of apparent sulfur oxidation activity of thermophilic archaea in bioleaching of chalcopyrite[J].Transactions of Nonferrous Metals Society of China,2013,23(8):2383-2388.
[26]ZENG Wei-min,QIU Guan-zhou,ZHOU Hong-bo,PENG Juan-hua,CHEN Miao,TAN Su Nee,CHAO Wei-liang,LIU Xue-duan,ZHANG Yan-sheng.Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate[J].Bioresource Technology,2010,101(18):7068-7075.
[27]ZHU Wei,XIA Jin-lan,YANG Yi,NIE Zhen-yuan,PENG An-an,LIU Hong-chang,QIU Guan-zhou.Thermophilic archaeal community succession and function change associated with the leaching rate in bioleaching of chalcopyrite[J].Bioresource Technology,2013,133:405-413.
[28]SONG Jian,LIN Jian-qun,REN Yi-lin,LIN Jian-qiang.Competitive adsorption of binary mixture of Leptospirillum ferriphilum and Acidithiobacillus caldus onto pyrite[J].Biotechnology and Bioprocess Engineering,2010,15(6):923-930.